Mercurial > repos > devteam > fastqc
diff test-data/fastqc_customlimits.txt @ 10:a00a6402d09a draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 2bfbb5ae6b801e43355fdc3f964a5111fe3fe3a1
author | iuc |
---|---|
date | Wed, 08 Feb 2017 12:43:43 -0500 |
parents | 8fae48caaf06 |
children | 3e1cdf5406db |
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--- a/test-data/fastqc_customlimits.txt Wed Nov 02 16:12:51 2016 -0400 +++ b/test-data/fastqc_customlimits.txt Wed Feb 08 12:43:43 2017 -0500 @@ -1,84 +1,84 @@ -# For each of the modules you can choose to not run that -# module at all by setting the value below to 1 for the -# modules you want to remove. -duplication ignore 1 -kmer ignore 0 -n_content ignore 0 -overrepresented ignore 0 -quality_base ignore 0 -sequence ignore 0 -gc_sequence ignore 0 -quality_sequence ignore 1 -tile ignore 1 -sequence_length ignore 0 -adapter ignore 0 - -# For the duplication module the value is the percentage -# remaining after deduplication. Measured levels below -# these limits trigger the warning / error. -duplication warn 70 -duplication error 50 - -# For the kmer module the filter is on the -log10 binomial -# pvalue for the most significant Kmer, so 5 would be -# 10^-5 = p<0.00001 -kmer warn 2 -kmer error 5 - -# For the N module the filter is on the percentage of Ns -# at any position in the library -n_content warn 5 -n_content error 20 - -# For the overrepresented seqs the warn value sets the -# threshold for the overrepresented sequences to be reported -# at all as the proportion of the library which must be seen -# as a single sequence -overrepresented warn 0.001 -overrepresented error 1 - -# The per base quality filter uses two values, one for the value -# of the lower quartile, and the other for the value of the -# median quality. Failing either of these will trigger the alert -quality_base_lower warn 10 -quality_base_lower error 5 -quality_base_median warn 50 -quality_base_median error 20 - -# The per base sequence content module tests the maximum deviation -# between A and T or C and G -sequence warn 2 -sequence error 5 - -# The per sequence GC content tests the maximum deviation between -# the theoretical distribution and the real distribution -gc_sequence warn 15 -gc_sequence error 30 - -# The per sequence quality module tests the phred score which is -# most frequently observed -quality_sequence warn 27 -quality_sequence error 20 - -# The per tile module tests the maximum phred score loss between -# and individual tile and the average for that base across all tiles -tile warn 5 -tile error 10 - -# The sequence length module tests are binary, so the values here -# simply turn them on or off. The actual tests warn if you have -# sequences of different length, and error if you have sequences -# of zero length. - -sequence_length warn 1 -sequence_length error 1 - -# The adapter module's warnings and errors are based on the -# percentage of reads in the library which have been observed -# to contain an adapter associated Kmer at any point - -adapter warn 5 -adapter error 10 - - +# For each of the modules you can choose to not run that +# module at all by setting the value below to 1 for the +# modules you want to remove. +duplication ignore 1 +kmer ignore 0 +n_content ignore 0 +overrepresented ignore 0 +quality_base ignore 0 +sequence ignore 0 +gc_sequence ignore 0 +quality_sequence ignore 1 +tile ignore 1 +sequence_length ignore 0 +adapter ignore 0 + +# For the duplication module the value is the percentage +# remaining after deduplication. Measured levels below +# these limits trigger the warning / error. +duplication warn 70 +duplication error 50 + +# For the kmer module the filter is on the -log10 binomial +# pvalue for the most significant Kmer, so 5 would be +# 10^-5 = p<0.00001 +kmer warn 2 +kmer error 5 + +# For the N module the filter is on the percentage of Ns +# at any position in the library +n_content warn 5 +n_content error 20 + +# For the overrepresented seqs the warn value sets the +# threshold for the overrepresented sequences to be reported +# at all as the proportion of the library which must be seen +# as a single sequence +overrepresented warn 0.001 +overrepresented error 1 + +# The per base quality filter uses two values, one for the value +# of the lower quartile, and the other for the value of the +# median quality. Failing either of these will trigger the alert +quality_base_lower warn 10 +quality_base_lower error 5 +quality_base_median warn 50 +quality_base_median error 20 + +# The per base sequence content module tests the maximum deviation +# between A and T or C and G +sequence warn 2 +sequence error 5 + +# The per sequence GC content tests the maximum deviation between +# the theoretical distribution and the real distribution +gc_sequence warn 15 +gc_sequence error 30 + +# The per sequence quality module tests the phred score which is +# most frequently observed +quality_sequence warn 27 +quality_sequence error 20 + +# The per tile module tests the maximum phred score loss between +# and individual tile and the average for that base across all tiles +tile warn 5 +tile error 10 + +# The sequence length module tests are binary, so the values here +# simply turn them on or off. The actual tests warn if you have +# sequences of different length, and error if you have sequences +# of zero length. + +sequence_length warn 1 +sequence_length error 1 + +# The adapter module's warnings and errors are based on the +# percentage of reads in the library which have been observed +# to contain an adapter associated Kmer at any point + +adapter warn 5 +adapter error 10 + + \ No newline at end of file