--- a/test-data/fastqc_data.txt	Fri May 10 14:23:53 2019 -0400
+++ b/test-data/fastqc_data.txt	Thu May 16 07:45:56 2019 -0400
@@ -1,803 +1,259 @@
-##FastQC	0.11.6
+##FastQC	0.11.8
 >>Basic Statistics	pass
 #Measure	Value
-Filename	1000gsample_fastq_bz2.bz2
+Filename	1000trimmed_fastq
 File type	Conventional base calls
 Encoding	Sanger / Illumina 1.9
-Total Sequences	5000
+Total Sequences	4905
 Sequences flagged as poor quality	0
-Sequence length	54
-%GC	43
->>END_MODULE
->>Per base sequence quality	warn
-#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
-1	28.9946	31.0	27.0	33.0	22.0	34.0
-2	28.486	30.0	26.0	32.0	21.0	33.0
-3	28.5272	30.0	26.0	32.0	21.0	34.0
-4	28.6252	30.0	26.0	32.0	22.0	33.0
-5	28.4804	30.0	26.0	32.0	21.0	33.0
-6	28.5128	30.0	26.0	32.0	22.0	33.0
-7	28.4102	30.0	26.0	32.0	21.0	33.0
-8	28.4674	30.0	26.0	32.0	22.0	33.0
-9	28.182	30.0	26.0	32.0	21.0	33.0
-10	28.0908	30.0	26.0	32.0	21.0	33.0
-11	28.1876	30.0	26.0	32.0	21.0	33.0
-12	28.1742	30.0	26.0	32.0	21.0	33.0
-13	27.923	30.0	25.0	32.0	20.0	33.0
-14	27.8984	29.0	25.0	32.0	21.0	33.0
-15	27.6112	29.0	25.0	32.0	20.0	33.0
-16	27.6638	29.0	25.0	32.0	20.0	33.0
-17	27.5734	29.0	25.0	32.0	20.0	33.0
-18	27.6348	29.0	25.0	32.0	20.0	33.0
-19	27.6526	29.0	25.0	32.0	20.0	33.0
-20	27.2716	29.0	24.0	32.0	19.0	33.0
-21	27.2642	29.0	25.0	31.0	19.0	33.0
-22	26.881	28.0	24.0	31.0	19.0	33.0
-23	26.8124	28.0	24.0	31.0	19.0	33.0
-24	26.8204	28.0	24.0	31.0	19.0	33.0
-25	26.6606	28.0	24.0	31.0	18.0	33.0
-26	26.3806	28.0	23.0	31.0	18.0	33.0
-27	25.947	28.0	23.0	31.0	17.0	33.0
-28	25.7446	27.0	22.0	31.0	16.0	33.0
-29	25.6856	27.0	22.0	31.0	16.0	33.0
-30	25.5228	27.0	22.0	31.0	16.0	33.0
-31	25.354	27.0	22.0	30.0	16.0	32.0
-32	25.1656	27.0	22.0	30.0	16.0	32.0
-33	25.0164	26.0	21.0	30.0	15.0	32.0
-34	24.75	26.0	21.0	30.0	15.0	32.0
-35	24.3926	26.0	21.0	30.0	15.0	32.0
-36	24.1122	25.0	20.0	30.0	14.0	32.0
-37	23.8612	25.0	20.0	29.0	13.0	32.0
-38	23.7544	25.0	20.0	29.0	13.0	32.0
-39	23.485	25.0	19.0	29.0	13.0	32.0
-40	23.1984	24.0	19.0	29.0	13.0	32.0
-41	23.0976	24.0	19.0	29.0	13.0	32.0
-42	22.9212	24.0	19.0	28.0	13.0	31.0
-43	22.5172	24.0	18.0	28.0	12.0	31.0
-44	22.3234	23.0	18.0	28.0	12.0	31.0
-45	22.1372	23.0	18.0	28.0	11.0	31.0
-46	22.2166	23.0	18.0	28.0	11.0	31.0
-47	22.075	23.0	18.0	28.0	11.0	31.0
-48	21.4592	22.0	17.0	27.0	11.0	31.0
-49	21.0954	22.0	16.0	27.0	10.0	30.0
-50	20.9356	22.0	16.0	27.0	10.0	30.0
-51	20.6152	21.0	15.0	27.0	9.0	30.0
-52	20.6588	21.0	16.0	27.0	9.0	30.0
-53	19.5852	20.0	14.0	25.0	9.0	29.0
-54	19.9424	21.0	14.0	26.0	7.0	30.0
+Sequence length	1-108
+%GC	40
 >>END_MODULE
->>Per tile sequence quality	pass
-#Tile	Base	Mean
-1	1	0.4542485249902164
-1	2	0.02493171505959424
-1	3	0.004493134032774293
-1	4	0.6119404948560927
-1	5	0.5283275008594686
-1	6	0.3741933003058371
-1	7	-0.2985554485298749
-1	8	0.6040181642739206
-1	9	0.43795745033596845
-1	10	0.05327706110314523
-1	11	0.8097461135057031
-1	12	0.9171964750225641
-1	13	1.0716973449965757
-1	14	0.562649938864773
-1	15	0.5112535943737946
-1	16	0.7098461441939676
-1	17	0.3989886413671577
-1	18	0.2351551176870892
-1	19	0.002716666680861124
-1	20	-0.5270840838615776
-1	21	0.08959510509382795
-1	22	-0.6322238602545553
-1	23	-0.8566085860715056
-1	24	-0.8167218970288026
-1	25	0.7728199138173544
-1	26	-0.26898877195552373
-1	27	-0.7734079986509634
-1	28	0.46313879073469266
-1	29	-1.0803714983510417
-1	30	-0.9107347156963463
-1	31	-1.1279249650605188
-1	32	0.03588141895047414
-1	33	-0.3276409211703317
-1	34	0.07833890179158587
-1	35	-0.8393131051954583
-1	36	-1.024889600311596
-1	37	-1.4177418858492992
-1	38	-1.912918888443187
-1	39	-1.880569234686881
-1	40	-0.9901560786471286
-1	41	-1.9499635516515283
-1	42	-2.3754765893384793
-1	43	-1.6682056704307335
-1	44	-2.2432919652101226
-1	45	-2.1214550638335794
-1	46	-1.6941624871292404
-1	47	-2.261740586778952
-1	48	-0.47246064535067234
-1	49	-1.345180096970381
-1	50	-1.3012642855865373
-1	51	-1.6748103331735038
-1	52	-1.4936238782530324
-1	53	-0.9900326328970834
-1	54	-0.9042724595665739
-2	1	0.27305194379363584
-2	2	-0.40803531790744074
-2	3	-1.054114924575284
-2	4	0.02463890755450393
-2	5	-1.0899875174555511
-2	6	-0.7630472369346997
-2	7	-1.2563088062832328
-2	8	0.09022085047660511
-2	9	-0.8821890698105506
-2	10	-1.0053309975049132
-2	11	-1.2879339841743942
-2	12	-2.149225991399902
-2	13	-1.2225639492647176
-2	14	-0.7472401710253394
-2	15	-0.9942409111207091
-2	16	-1.07061783627001
-2	17	-1.0825742401957257
-2	18	-1.0554431729112004
-2	19	-1.374084310120118
-2	20	-0.25260300938050406
-2	21	-0.7384879229891972
-2	22	-0.5973032253339206
-2	23	-1.926205655668575
-2	24	-1.8018256821325878
-2	25	-1.2574609164634758
-2	26	-2.588402691369442
-2	27	-1.909427534670499
-2	28	-2.213051685455781
-2	29	-3.343130961110507
-2	30	-3.447731052692685
-2	31	-3.261502498638052
-2	32	-2.596108813039759
-2	33	-2.173306366835778
-2	34	-2.968059144606464
-2	35	-3.110863776746129
-2	36	-3.6207381961601897
-2	37	-2.8287308968383122
-2	38	-2.3431997187240157
-2	39	-2.7157340698517167
-2	40	-2.8763587648498152
-2	41	-3.221758423446399
-2	42	-3.735916149778042
-2	43	-2.8279126301376927
-2	44	-2.034012355930514
-2	45	-3.745875088253605
-2	46	-3.8848828778496305
-2	47	-3.6639383889767565
-2	48	-3.0197133926034176
-2	49	-3.1119688637591487
-2	50	-2.7486391329613866
-2	51	-2.4279238862870542
-2	52	-3.3607789454081
-2	53	-4.239849482713934
-2	54	-3.3794861347802474
-3	1	0.4228103978999158
-3	2	1.0474512245356244
-3	3	0.4013716178677811
-3	4	-0.7648710993468057
-3	5	1.642931737202833
-3	6	0.42190790454218075
-3	7	0.5808202482371243
-3	8	0.22341615758495692
-3	9	0.06605109582092084
-3	10	1.203778733343949
-3	11	-0.3886931284117985
-3	12	0.31385199341721304
-3	13	0.7829570997345918
-3	14	-0.2772608749590866
-3	15	-0.2613216564623251
-3	16	-0.6475674566978924
-3	17	-1.0549689951991752
-3	18	-1.3066509023798005
-3	19	-0.22225546263909735
-3	20	-1.028310393783542
-3	21	-0.3112967566675984
-3	22	-0.2177311066313692
-3	23	1.415409251163723
-3	24	1.0256415366389788
-3	25	-0.5776817584234486
-3	26	0.41573809538003914
-3	27	1.098163907703551
-3	28	0.5095156023288965
-3	29	0.6313341872676865
-3	30	0.4032006243259474
-3	31	-0.21733410664357322
-3	32	-0.1723683023427256
-3	33	-0.8558460493754616
-3	34	0.4094425807213504
-3	35	1.0478663819840293
-3	36	2.193616531238014
-3	37	0.2458032646523698
-3	38	0.5829562509102146
-3	39	-0.23954359366124223
-3	40	-0.6762207386248349
-3	41	1.2420096924956283
-3	42	1.0798188398699935
-3	43	0.9132881980196572
-3	44	0.7368641105981268
-3	45	0.004297444527622929
-3	46	1.1033848930268384
-3	47	0.008939457814136631
-3	48	-1.3137092518166682
-3	49	-0.5681455373271262
-3	50	1.1098839864312993
-3	51	-0.835791381111072
-3	52	-0.5478044802597211
-3	53	-0.49036708105762017
-3	54	0.06495830966419547
-4	1	0.05082972157141441
-4	2	0.528472618600496
-4	3	-0.06998794044829992
-4	4	0.9770198599354565
-4	5	1.1004886730206387
-4	6	0.1575876837002177
-4	7	0.6801991302247039
-4	8	0.5346652949210515
-4	9	-0.2155224031438827
-4	10	-0.29104528321919787
-4	11	-0.7165054127458248
-4	12	-1.561924404098317
-4	13	-0.5241512508520181
-4	14	-0.19168461546978222
-4	15	-0.91487583175563
-4	16	0.42144565579348026
-4	17	0.7586956010741162
-4	18	-0.26179237926040955
-4	19	0.2449633089275025
-4	20	0.5093017525242587
-4	21	-0.3575355420368176
-4	22	-1.5020651300958257
-4	23	-0.19604692550984382
-4	24	-0.008174888481793374
-4	25	1.0917454327428722
-4	26	0.7131846102178585
-4	27	0.2493026240596592
-4	28	0.9774245050204087
-4	29	-0.45424207222161783
-4	30	-1.4953501003117324
-4	31	0.2146879775524262
-4	32	0.9277007107697663
-4	33	-0.26854446207387284
-4	34	-0.30139247793979607
-4	35	0.5240568581745073
-4	36	-0.5572461326681264
-4	37	0.3934913253839092
-4	38	0.4345780590537629
-4	39	1.0779167237990777
-4	40	0.3299904414993904
-4	41	0.39728919560121767
-4	42	-0.5454399593018522
-4	43	-2.6850554872805503
-4	44	0.7913844694663119
-4	45	0.8255534831749678
-4	46	0.5436885507218001
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-4	54	-0.23662899192310505
-5	1	0.6850177557594499
-5	2	0.48647017659805414
-5	3	0.7275700571096984
-5	4	0.38117126408686275
-5	5	1.0514044239363898
-5	6	1.066500992613527
-5	7	0.9475983976239704
-5	8	-0.05752029726454211
-5	9	1.0994959118744312
-5	10	0.6532770611031467
-5	11	0.17897688273647105
-5	12	0.1325810904071787
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-5	14	0.1318807080955402
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-5	18	0.9890012715332439
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-5	52	0.4756068909777369
-5	53	0.2868904440259925
-5	54	-1.0119647672588812
-6	1	0.9498196205613141
-6	2	-1.07903098890311
-6	3	0.2142833438229843
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-8	8	-0.3584252746401084
-8	9	-0.09507422387217446
-8	10	-1.100569092743008
-8	11	-0.5250955154535717
-8	12	0.009504167330256053
-8	13	1.1938692906979327
-8	14	1.000658988638527
-8	15	0.6402128703918954
-8	16	1.2944615288093537
-8	17	-0.04942764822560264
-8	18	1.183118918592065
-8	19	0.842535671205745
-8	20	0.47382089351398804
-8	21	-0.23755421617313743
-8	22	-0.6145768014310278
-8	23	-0.7231244231755767
-8	24	-0.6094820780242785
-8	25	-0.5968633441011981
-8	26	0.4984320425241151
-8	27	0.27591326831736396
-8	28	-0.3545082680888356
-8	29	0.7847868726896827
-8	30	1.3575910761588545
-8	31	2.432708519102377
-8	32	0.17886784428984015
-8	33	1.3402323819970903
-8	34	0.3045832456829878
-8	35	-0.4031140101728319
-8	36	0.3619882277427031
-8	37	0.4401766661868969
-8	38	1.317397853638262
-8	39	0.5447701318289546
-8	40	-0.6924185220860437
-8	41	1.582163145181049
-8	42	1.4489577998017893
-8	43	0.8919753250443812
-8	44	1.0173415189527724
-8	45	1.1654227642207182
-8	46	1.5194121735042465
-8	47	2.5545490059812295
-8	48	1.6777655989932185
-8	49	0.3380778215816562
-8	50	1.5263375243682127
-8	51	-0.8929098806848152
-8	52	1.233525443013935
-8	53	0.5262569598630975
-8	54	2.859075956723018
-9	1	-1.2602813895396956
-9	2	-0.5699400798122021
-9	3	-0.4160196864800447
-9	4	-0.2769483940327966
-9	5	-1.8391938666619012
-9	6	0.16393689004942402
-9	7	0.004008654034226566
-9	8	-0.611366451110694
-9	9	-0.7996493872708683
-9	10	-0.9450135371874531
-9	11	1.1088914126510012
-9	12	1.7428375006635903
-9	13	0.1964836697829
-9	14	-1.0424782662634335
-9	15	0.3676638507840515
-9	16	-1.349982915635092
-9	17	0.45710829948681564
-9	18	-0.5443320618000911
-9	19	-0.16138589742170595
-9	20	0.4045398477623543
-9	21	-0.03690062140189809
-9	22	0.6566650286343325
-9	23	1.2801435506806307
-9	24	1.0140473337404288
-9	25	1.0155549565523962
-9	26	-0.4963391993059503
-9	27	-1.144348169591133
-9	28	-0.29241676482086376
-9	29	0.018773800794257056
-9	30	0.5935387885771561
-9	31	-0.04245487959043359
-9	32	1.076907059976115
-9	33	1.2108206172912048
-9	34	0.16209958555226933
-9	35	-0.3299113957937472
-9	36	0.6554522800302856
-9	37	0.16174529363787826
-9	38	-0.29240606793036505
-9	39	-0.46176581588346366
-9	40	-0.17477146326251614
-9	41	-0.3995362012241799
-9	42	0.40059178672989404
-9	43	-0.7929919952170579
-9	44	-0.9578218797400382
-9	45	0.6699979276194128
-9	46	0.124640931674179
-9	47	0.2408235157851486
-9	48	-0.37844355133357865
-9	49	0.3419993902091072
-9	50	-0.8756232599455132
-9	51	0.44826658990341883
-9	52	0.34398295935380574
-9	53	0.5347536918892395
-9	54	-1.9350416903358045
-10	1	1.0952741660158587
-10	2	1.4967265868544644
-10	3	1.317313646853286
-10	4	-0.3547261718105723
-10	5	-1.4614160888841212
-10	6	0.29727022338276043
-10	7	0.570675320700893
-10	8	0.7553002155559696
-10	9	-0.11076049838197832
-10	10	0.9660975739236584
-10	11	1.3200025237621134
-10	12	0.5761708339969225
-10	13	1.1742614475606779
-10	14	0.49085506706990145
-10	15	0.6676638507840522
-10	16	0.5944615288093544
-10	17	-0.2984472560687408
-10	18	-0.07766539513342252
-10	19	0.871947435911629
-10	20	0.6934287366512422
-10	21	-0.4924561769574538
-10	22	0.13444280641211037
-10	23	1.3356991062361878
-10	24	0.6140473337404266
-10	25	0.1933327343301734
-10	26	1.2592163562496026
-10	27	2.2112073859644212
-10	28	1.7964721240680284
-10	29	0.7632182452387006
-10	30	1.604649899688269
-10	31	1.8797673426317907
-10	32	0.47690705997611715
-10	33	-0.15584604937545876
-10	34	1.8398773633300465
-10	35	2.981199715317363
-10	36	0.8110078355858406
-10	37	1.8617452936378776
-10	38	1.7742605987363014
-10	39	3.2937897396720928
-10	40	3.2918952034041524
-10	41	1.3115749098869323
-10	42	2.911702897841007
-10	43	2.9292302270051636
-10	44	1.964400342482186
-10	45	0.5477757053971892
-10	46	1.9135298205630704
-10	47	1.2741568491184836
-10	48	2.799334226444202
-10	49	3.441999390209105
-10	50	4.391043406721156
-10	51	3.7815999232367545
-10	52	1.3217607371315836
-10	53	3.0125314696670173
-10	54	2.2316249763308633
+>>Per base sequence quality	pass
+#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
+1	29.282772680937818	31.0	27.0	33.0	23.0	34.0
+2	28.832925336597306	30.0	27.0	32.0	22.0	33.0
+3	28.896953588223266	30.0	27.0	32.0	23.0	34.0
+4	29.006785934608267	30.0	27.0	32.0	23.0	34.0
+5	28.9377990430622	30.0	27.0	32.0	22.0	34.0
+6	29.002938706968934	30.0	27.0	32.0	23.0	34.0
+7	28.899830220713074	30.0	27.0	32.0	22.0	34.0
+8	28.900706184463942	30.0	27.0	32.0	22.0	33.0
+9	28.754602555772145	30.0	27.0	32.0	22.0	34.0
+10-11	28.822083022456294	30.0	27.0	32.0	22.5	33.0
+12-13	28.74898578710789	30.0	26.5	32.0	22.0	33.0
+14-15	28.53370750727077	30.0	26.0	32.0	22.0	33.5
+16-17	28.52661127583153	30.0	26.0	32.0	22.0	33.0
+18-19	28.68343192244741	30.0	26.0	32.0	22.5	33.0
+20-21	28.370130166336985	29.5	26.0	32.0	22.0	33.0
+22-23	28.185946266098476	29.0	26.0	32.0	22.0	33.0
+24-25	28.13009469533431	29.0	26.0	32.0	21.5	33.0
+26-27	28.075768267577	29.0	26.0	32.0	21.5	33.0
+28-29	27.828024188665616	29.0	25.0	32.0	21.0	33.0
+30-31	27.792826876513317	29.0	25.0	31.5	21.0	33.0
+32-33	27.490989073616355	28.0	25.0	31.0	21.0	33.0
+34-35	27.357199679261562	28.0	24.5	31.0	21.0	33.0
+36-37	27.062158129410527	28.0	24.0	31.0	20.5	33.0
+38-39	27.238022216783758	28.0	24.0	31.0	21.0	33.0
+40-41	27.028963434226593	28.0	24.0	31.0	20.5	33.0
+42-43	26.90862078496675	27.0	24.0	31.0	20.5	33.0
+44-45	26.632003369751054	27.0	24.0	30.0	20.0	32.0
+46-47	26.83042786373109	27.5	24.0	31.0	20.5	32.0
+48-49	26.431681832255798	27.0	23.5	30.0	20.0	32.0
+50-51	26.219000735104444	27.0	23.0	30.0	20.0	32.0
+52-53	25.925690423864786	26.5	22.5	29.5	19.5	31.5
+54-55	27.995241016389798	29.5	25.5	31.5	20.5	33.0
+56-57	30.410857897242785	31.5	28.5	33.0	25.5	34.0
+58-59	30.4083885209713	31.5	28.5	33.0	25.5	34.0
+60-61	30.444812362030905	31.5	29.0	33.0	26.0	34.0
+62-63	30.44039735099338	31.5	29.0	33.0	25.5	34.0
+64-65	30.441712697003883	31.5	29.0	33.0	25.5	34.0
+66-67	30.51141384388807	32.0	29.0	33.0	25.5	34.0
+68-69	30.05117820324006	31.0	28.0	33.0	24.5	34.0
+70-71	30.276141384388808	31.0	29.0	33.0	25.0	34.0
+72-73	30.385493372606774	31.5	29.0	33.0	25.5	34.0
+74-75	30.213549337260677	31.0	28.5	33.0	25.5	34.0
+76-77	29.678939617083948	31.0	28.0	33.0	24.5	34.0
+78-79	30.019145802650957	31.0	28.0	33.0	24.5	34.0
+80-81	29.664212076583212	31.0	27.5	33.0	24.0	34.0
+82-83	29.653534609720175	31.0	28.0	32.5	24.5	34.0
+84-85	29.425625920471283	30.5	27.0	32.0	24.0	34.0
+86-87	29.34057437407953	30.0	27.0	32.5	24.0	34.0
+88-89	28.785714285714285	30.0	27.0	32.0	22.5	33.5
+90-91	28.667525773195877	29.5	26.0	32.0	23.0	33.0
+92-93	28.565905743740796	29.0	26.0	32.0	23.0	33.0
+94-95	28.271723122238583	29.0	26.0	32.0	22.5	33.0
+96-97	27.94955817378498	29.0	25.5	31.0	22.0	33.0
+98-99	27.641132075471695	28.0	25.0	31.0	21.5	33.0
+100-101	27.614716981132077	28.0	25.0	31.0	21.5	33.0
+102-103	27.196226415094337	28.0	24.5	31.0	21.0	32.5
+104-105	26.870943396226416	27.5	24.0	31.0	20.0	32.5
+106-107	26.367593937711003	27.0	23.5	30.0	20.5	32.0
+108	27.5650623885918	28.0	24.0	31.0	22.0	33.0
 >>END_MODULE
 >>Per sequence quality scores	warn
 #Quality	Count
-8	4.0
-9	4.0
-10	2.0
-11	2.0
-12	4.0
-13	6.0
-14	4.0
-15	11.0
-16	20.0
-17	50.0
-18	98.0
-19	149.0
-20	210.0
-21	275.0
-22	367.0
-23	425.0
-24	518.0
-25	540.0
-26	539.0
-27	532.0
-28	468.0
-29	360.0
-30	234.0
-31	122.0
-32	51.0
-33	5.0
+20	7.0
+21	24.0
+22	47.0
+23	78.0
+24	226.0
+25	513.0
+26	830.0
+27	1017.0
+28	947.0
+29	645.0
+30	352.0
+31	157.0
+32	55.0
+33	6.0
+34	1.0
 >>END_MODULE
 >>Per base sequence content	pass
 #Base	G	A	T	C
-1	21.5	32.64	25.919999999999998	19.939999999999998
-2	24.04	31.72	26.540000000000003	17.7
-3	21.58	28.860000000000003	29.42	20.14
-4	21.58	28.28	28.9	21.240000000000002
-5	21.16	29.720000000000002	28.32	20.8
-6	22.38	29.24	25.72	22.66
-7	21.34	30.320000000000004	28.24	20.1
-8	21.58	29.060000000000002	27.939999999999998	21.42
-9	20.3	28.48	28.58	22.64
-10	21.2	30.84	26.56	21.4
-11	21.14	28.64	27.279999999999998	22.939999999999998
-12	21.32	28.88	28.999999999999996	20.8
-13	20.36	29.220000000000002	28.939999999999998	21.48
-14	20.02	29.2	29.220000000000002	21.560000000000002
-15	20.200000000000003	29.220000000000002	28.38	22.2
-16	20.34	29.38	29.299999999999997	20.979999999999997
-17	20.599999999999998	29.14	28.499999999999996	21.759999999999998
-18	20.599999999999998	27.92	29.4	22.08
-19	21.66	28.46	28.58	21.3
-20	20.0	29.24	28.96	21.8
-21	21.72	28.499999999999996	28.18	21.6
-22	20.52	30.880000000000003	27.3	21.3
-23	20.72	28.9	29.720000000000002	20.66
-24	21.12	28.62	27.58	22.68
-25	21.099999999999998	29.060000000000002	29.160000000000004	20.68
-26	22.18887555022009	28.391356542617046	28.131252501000397	21.288515406162464
-27	21.344268853770753	28.525705141028208	28.765753150630125	21.364272854570913
-28	19.34	30.54	27.46	22.66
-29	20.90418083616723	29.12582516503301	29.045809161832363	20.924184836967395
-30	21.099999999999998	29.4	27.439999999999998	22.06
-31	20.84	28.04	28.660000000000004	22.46
-32	19.847939175670266	28.371348539415763	28.7515006002401	23.02921168467387
-33	22.084416883376676	28.62572514502901	27.265453090618124	22.024404880976196
-34	21.304260852170433	29.285857171434287	27.265453090618124	22.144428885777156
-35	21.084216843368676	29.425885177035404	28.225645129025807	21.264252850570113
-36	21.404280856171233	28.665733146629325	28.805761152230446	21.124224844968996
-37	20.888355342136855	29.43177270908363	28.53141256502601	21.1484593837535
-38	21.864372874574915	29.02580516103221	28.065613122624526	21.044208841768352
-39	21.928771508603443	28.251300520208083	27.050820328131252	22.769107643057225
-40	21.32	28.499999999999996	27.82	22.36
-41	21.673003802281368	28.116870122073244	27.61656994196518	22.59355613368021
-42	22.29337602561537	28.036822093255953	27.71662997798679	21.953171903141886
-43	21.244248849769953	28.425685137027408	27.825565113022606	22.504500900180034
-44	21.404280856171233	28.985797159431886	27.545509101820365	22.064412882576516
-45	21.86	29.28	26.740000000000002	22.12
-46	21.26	27.54	27.74	23.46
-47	21.628651460584233	28.271308523409367	28.051220488195277	22.048819527811126
-48	21.279999999999998	28.439999999999998	26.400000000000002	23.880000000000003
-49	21.044208841768352	29.045809161832363	27.185437087417487	22.724544908981798
-50	21.82873149259704	28.15126050420168	26.810724289715886	23.209283713485394
-51	21.028411364545818	27.73109243697479	28.011204481792717	23.229291716686674
-52	21.824364872974595	30.146029205841167	25.94518903780756	22.084416883376676
-53	21.164232846569313	28.325665133026607	28.045609121824366	22.464492898579717
-54	21.53907976692787	27.024311834438418	26.90375728350412	24.5328511151296
+1	21.223241590214066	33.00713557594291	25.97349643221203	19.79612640163099
+2	23.76580987352101	31.415748674010608	27.315381476948186	17.503059975520195
+3	21.876916785933346	28.70578613780413	29.46227765283173	19.95501942343079
+4	21.55048324079786	28.295290972650626	28.850503804236066	21.303721982315444
+5	21.219055543998337	30.205949656750576	28.271271063033076	20.303723736218014
+6	22.124265323257767	28.946263643996637	26.364399664147776	22.565071368597817
+7	21.943972835314092	29.9660441426146	28.395585738539896	19.69439728353141
+8	21.378129681147016	28.825165846351382	27.8621870318853	21.934517440616304
+9	20.077972709551656	29.088152479965345	28.654970760233915	22.17890405024908
+10-11	21.243237275035884	29.468919068124105	27.680247322512976	21.607596334327038
+12-13	20.8276950834461	29.03698691926026	29.18358141632837	20.951736580965267
+14-15	20.24001846295869	28.975305792753286	29.471497807523654	21.313177936764365
+16-17	19.83872880351002	29.396418830783826	29.621724178821296	21.14312818688486
+18-19	20.845552297165202	27.871456500488755	29.973118279569892	21.309872922776147
+20-21	20.538172715894866	28.973717146433042	29.111389236545683	21.376720901126408
+22-23	20.88082901554404	30.129533678756477	29.300518134715027	19.689119170984455
+24-25	20.506974248927037	29.07725321888412	29.05042918454936	21.365343347639485
+26-27	20.86603139013453	28.054932735426007	30.339125560538115	20.739910313901344
+28-29	19.32600732600733	30.842490842490843	28.776556776556777	21.054945054945055
+30-31	20.638820638820636	29.19226044226044	29.49938574938575	20.669533169533167
+32-33	19.47989016314004	29.009853012437407	29.38136003876595	22.1288967856566
+34-35	19.6674020023757	29.713219073477003	30.205328355676226	20.41405056847107
+36-37	20.443482963764197	29.853975121687398	30.61114115738237	19.091400757166035
+38-39	20.992661259173424	28.041714947856317	30.088837388953266	20.876786404016993
+40-41	19.860197368421055	28.680098684210524	30.32483552631579	21.134868421052634
+42-43	20.01321003963012	27.873183619550858	30.823425803610743	21.29018053720828
+44-45	18.941371943982908	30.192262046047947	29.954901495371466	20.911464514597675
+46-47	20.1888718734048	28.126595201633485	31.470137825421133	20.21439509954058
+48-49	19.56159822419534	29.689234184239734	28.77358490566038	21.975582685904552
+50-51	20.50656087885261	28.2575526396094	30.759841318278912	20.47604516325908
+52-53	17.691256830601095	29.918032786885245	31.864754098360653	20.525956284153004
+54-55	17.214912280701753	32.30994152046784	29.53216374269006	20.942982456140353
+56-57	19.30856932695844	30.893710923133504	30.893710923133504	18.90400882677455
+58-59	20.014716703458426	29.72774098601913	31.42016188373804	18.8373804267844
+60-61	17.402501839587934	33.18616629874908	29.506990434142754	19.904341427520237
+62-63	18.543046357615893	29.72774098601913	32.2663723325975	19.462840323767477
+64-65	16.893632683106365	32.94074346705926	30.180345969819655	19.985277880014724
+66-67	18.519882179675996	28.902798232695137	32.80559646539028	19.771723122238587
+68-69	17.2680412371134	30.375552282768776	31.66421207658321	20.692194403534607
+70-71	17.673048600883654	27.798232695139912	35.34609720176731	19.182621502209134
+72-73	16.200294550810014	29.528718703976438	33.87334315169367	20.39764359351988
+74-75	18.372606774668633	28.019145802650957	34.5360824742268	19.072164948453608
+76-77	16.678939617083945	31.70103092783505	31.73784977908689	19.88217967599411
+78-79	18.77761413843888	28.387334315169365	32.62150220913107	20.213549337260677
+80-81	16.384388807069218	30.927835051546392	32.9160530191458	19.771723122238587
+82-83	17.083946980854197	30.927835051546392	32.54786450662739	19.44035346097202
+84-85	16.936671575846834	31.590574374079527	31.443298969072163	20.029455081001473
+86-87	17.488954344624446	27.908689248895435	34.20471281296024	20.39764359351988
+88-89	16.789396170839467	32.36377025036819	31.075110456553755	19.771723122238587
+90-91	19.587628865979383	29.749631811487482	33.5419734904271	17.120765832106038
+92-93	17.746686303387335	30.04418262150221	31.33284241531664	20.876288659793815
+94-95	18.924889543446245	28.460972017673047	33.39469808541973	19.219440353460975
+96-97	16.42120765832106	29.418262150220915	32.47422680412371	21.686303387334316
+98-99	18.158090976882924	29.34377330350485	33.1469052945563	19.351230425055927
+100-101	17.77358490566038	29.849056603773583	33.28301886792453	19.09433962264151
+102-103	18.339622641509436	29.509433962264154	30.90566037735849	21.245283018867926
+104-105	18.037735849056606	28.67924528301887	31.77358490566038	21.50943396226415
+106-107	17.2782874617737	29.357798165137616	33.52446483180428	19.839449541284402
+108	20.053475935828878	26.38146167557932	31.72905525846702	21.836007130124777
 >>END_MODULE
 >>Per sequence GC content	warn
 #GC Content	Count
-0	3.0
-1	5.0
-2	7.0
-3	7.0
-4	7.0
-5	5.0
-6	2.0
-7	1.0
-8	1.5
-9	2.0
-10	1.5
-11	1.0
-12	2.5
-13	4.0
-14	4.0
-15	4.0
-16	5.0
-17	6.0
-18	11.0
-19	19.5
-20	23.0
-21	37.5
-22	52.0
-23	61.0
-24	70.0
-25	79.0
-26	88.0
-27	117.5
-28	147.0
-29	159.5
-30	172.0
-31	203.0
-32	243.0
-33	252.0
-34	290.0
-35	328.0
-36	330.0
-37	332.0
-38	332.5
-39	333.0
-40	342.0
-41	351.0
-42	343.5
-43	336.0
-44	318.5
-45	279.5
-46	258.0
-47	254.5
-48	251.0
-49	234.5
-50	218.0
-51	209.5
-52	201.0
-53	198.5
-54	196.0
-55	177.0
-56	162.5
-57	167.0
-58	147.0
-59	127.0
-60	106.5
-61	86.0
-62	85.0
-63	84.0
-64	61.5
-65	39.0
-66	42.5
-67	46.0
-68	34.0
-69	17.0
-70	12.0
-71	14.0
-72	16.0
-73	12.5
-74	9.0
-75	6.5
-76	4.0
-77	4.5
-78	5.0
-79	5.0
-80	5.0
-81	3.0
-82	1.0
-83	1.0
-84	1.0
-85	1.0
-86	1.0
-87	1.0
-88	1.0
-89	1.0
-90	2.0
-91	3.0
-92	2.5
-93	2.0
-94	2.0
-95	3.0
-96	4.0
-97	5.0
-98	6.0
-99	3.5
-100	1.0
+0	15.0
+1	15.5
+2	16.5
+3	17.0
+4	18.0
+5	21.5
+6	26.5
+7	30.0
+8	33.5
+9	36.0
+10	41.0
+11	47.0
+12	47.5
+13	56.0
+14	65.5
+15	69.0
+16	72.5
+17	78.0
+18	86.5
+19	94.5
+20	105.5
+21	113.0
+22	123.0
+23	132.5
+24	149.5
+25	172.5
+26	200.0
+27	222.0
+28	247.0
+29	282.0
+30	310.5
+31	337.0
+32	370.0
+33	403.5
+34	438.0
+35	465.0
+36	481.5
+37	511.0
+38	523.5
+39	506.0
+40	491.0
+41	491.5
+42	482.0
+43	483.0
+44	487.5
+45	471.5
+46	471.0
+47	468.5
+48	473.5
+49	472.0
+50	437.5
+51	415.5
+52	407.5
+53	398.0
+54	381.0
+55	364.0
+56	349.0
+57	341.0
+58	330.5
+59	320.0
+60	320.5
+61	301.0
+62	275.0
+63	245.0
+64	207.0
+65	190.5
+66	182.0
+67	173.0
+68	167.0
+69	151.5
+70	131.5
+71	121.0
+72	117.5
+73	110.5
+74	104.0
+75	90.5
+76	75.0
+77	67.5
+78	62.5
+79	61.5
+80	59.5
+81	57.0
+82	54.5
+83	47.0
+84	39.5
+85	38.0
+86	36.5
+87	35.5
+88	28.0
+89	21.0
+90	19.5
+91	17.0
+92	15.5
+93	14.5
+94	13.5
+95	13.5
+96	15.0
+97	15.5
+98	17.0
+99	16.0
+100	13.5
 >>END_MODULE
 >>Per base N content	pass
 #Base	N-Count
@@ -810,69 +266,95 @@
 7	0.0
 8	0.0
 9	0.0
-10	0.0
-11	0.0
-12	0.0
-13	0.0
-14	0.0
-15	0.0
-16	0.0
-17	0.0
-18	0.0
-19	0.0
-20	0.0
-21	0.0
-22	0.0
-23	0.0
-24	0.0
-25	0.0
-26	0.04
-27	0.02
-28	0.0
-29	0.02
-30	0.0
-31	0.0
-32	0.04
-33	0.02
-34	0.02
-35	0.02
-36	0.02
-37	0.04
-38	0.02
-39	0.04
-40	0.0
-41	0.06
-42	0.06
-43	0.02
-44	0.02
-45	0.0
-46	0.0
-47	0.04
-48	0.0
-49	0.02
-50	0.04
-51	0.04
-52	0.02
-53	0.02
-54	0.45999999999999996
+10-11	0.0
+12-13	0.0
+14-15	0.0
+16-17	0.0
+18-19	0.0
+20-21	0.0
+22-23	0.0
+24-25	0.0
+26-27	0.0
+28-29	0.0
+30-31	0.0
+32-33	0.0
+34-35	0.0
+36-37	0.0
+38-39	0.0
+40-41	0.0
+42-43	0.0
+44-45	0.0
+46-47	0.0
+48-49	0.0
+50-51	0.0
+52-53	0.0
+54-55	0.0
+56-57	0.0
+58-59	0.0
+60-61	0.0
+62-63	0.0
+64-65	0.0
+66-67	0.0
+68-69	0.0
+70-71	0.0
+72-73	0.0
+74-75	0.0
+76-77	0.0
+78-79	0.0
+80-81	0.0
+82-83	0.0
+84-85	0.0
+86-87	0.0
+88-89	0.0
+90-91	0.0
+92-93	0.0
+94-95	0.0
+96-97	0.0
+98-99	0.0
+100-101	0.0
+102-103	0.0
+104-105	0.0
+106-107	0.0
+108	0.0
 >>END_MODULE
->>Sequence Length Distribution	pass
+>>Sequence Length Distribution	warn
 #Length	Count
-54	5000.0
+0-4	98.0
+5-9	250.0
+10-14	257.0
+15-19	279.0
+20-24	321.0
+25-29	396.0
+30-34	401.0
+35-39	433.0
+40-44	401.0
+45-49	385.0
+50-54	320.0
+55-59	5.0
+60-64	1.0
+65-69	0.0
+70-74	0.0
+75-79	0.0
+80-84	0.0
+85-89	0.0
+90-94	0.0
+95-99	33.0
+100-104	0.0
+105-109	1325.0
 >>END_MODULE
 >>Sequence Duplication Levels	pass
-#Total Deduplicated Percentage	99.52
+#Total Deduplicated Percentage	98.73598369011212
 #Duplication Level	Percentage of deduplicated	Percentage of total
-1	99.81913183279742	99.33999999999999
-2	0.08038585209003216	0.16
-3	0.04019292604501608	0.12
-4	0.0	0.0
-5	0.02009646302250804	0.1
+1	99.44249432170142	98.1855249745158
+2	0.47491224447656405	0.9378185524974516
+3	0.041296716911005574	0.12232415902140673
+4	0.020648358455502787	0.08154943934760449
+5	0.0	0.0
 6	0.0	0.0
-7	0.04019292604501608	0.27999999999999997
+7	0.0	0.0
 8	0.0	0.0
 9	0.0	0.0
->10	0.0	0.0
+>10	0.020648358455502787	0.672782874617737
 >50	0.0	0.0
 >100	0.0	0.0
 >500	0.0	0.0
@@ -882,52 +364,61 @@
 >>END_MODULE
 >>Overrepresented sequences	warn
 #Sequence	Count	Percentage	Possible Source
-CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (96% over 33bp)
-CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (100% over 35bp)
+ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT	33	0.672782874617737	No Hit
 >>END_MODULE
 >>Adapter Content	pass
 #Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
 1	0.0	0.0	0.0	0.0	0.0
-2	0.02	0.0	0.0	0.0	0.0
-3	0.02	0.0	0.0	0.0	0.0
-4	0.02	0.0	0.0	0.0	0.0
-5	0.02	0.0	0.0	0.0	0.0
-6	0.02	0.0	0.0	0.0	0.0
-7	0.02	0.0	0.0	0.0	0.0
-8	0.02	0.0	0.0	0.0	0.0
-9	0.02	0.0	0.0	0.0	0.0
-10	0.02	0.0	0.0	0.0	0.0
-11	0.02	0.0	0.0	0.0	0.0
-12	0.02	0.0	0.0	0.0	0.0
-13	0.02	0.0	0.0	0.0	0.0
-14	0.02	0.0	0.0	0.0	0.0
-15	0.02	0.0	0.0	0.0	0.0
-16	0.02	0.0	0.0	0.0	0.0
-17	0.02	0.0	0.0	0.0	0.0
-18	0.02	0.0	0.0	0.0	0.0
-19	0.06	0.0	0.0	0.0	0.0
-20	0.36	0.0	0.0	0.0	0.0
-21	0.36	0.0	0.0	0.0	0.0
-22	0.36	0.0	0.0	0.0	0.0
-23	0.36	0.0	0.0	0.0	0.0
-24	0.36	0.0	0.0	0.0	0.0
-25	0.4	0.0	0.0	0.0	0.0
-26	0.4	0.0	0.0	0.0	0.0
-27	0.42	0.0	0.0	0.0	0.0
-28	0.62	0.0	0.0	0.0	0.0
-29	0.66	0.0	0.0	0.0	0.0
-30	0.68	0.0	0.0	0.0	0.0
-31	0.68	0.0	0.0	0.0	0.0
-32	0.68	0.0	0.0	0.0	0.0
-33	0.68	0.0	0.0	0.0	0.0
-34	0.7	0.0	0.0	0.0	0.0
-35	0.76	0.0	0.0	0.0	0.0
-36	0.8	0.0	0.0	0.0	0.0
-37	0.88	0.0	0.0	0.0	0.0
-38	1.0	0.0	0.0	0.0	0.0
-39	1.12	0.0	0.0	0.0	0.0
-40	1.22	0.0	0.0	0.0	0.0
-41	1.38	0.0	0.0	0.0	0.0
-42	1.48	0.0	0.0	0.0	0.0
-43	1.68	0.0	0.0	0.0	0.0
+2	0.0	0.0	0.0	0.0	0.0
+3	0.0	0.0	0.0	0.0	0.0
+4	0.0	0.0	0.0	0.0	0.0
+5	0.0	0.0	0.0	0.0	0.0
+6	0.0	0.0	0.0	0.0	0.0
+7	0.0	0.0	0.0	0.0	0.0
+8	0.0	0.0	0.0	0.0	0.0
+9	0.0	0.0	0.0	0.0	0.0
+10-11	0.0	0.0	0.0	0.0	0.0
+12-13	0.0	0.0	0.0	0.0	0.0
+14-15	0.0	0.0	0.0	0.0	0.0
+16-17	0.0	0.0	0.0	0.0	0.0
+18-19	0.0	0.0	0.0	0.0	0.0
+20-21	0.12232415902140673	0.0	0.0	0.0	0.0
+22-23	0.12232415902140673	0.0	0.0	0.0	0.0
+24-25	0.12232415902140673	0.0	0.0	0.0	0.0
+26-27	0.1325178389398573	0.0	0.0	0.0	0.0
+28-29	0.2038735983690112	0.0	0.0	0.0	0.0
+30-31	0.22426095820591233	0.0	0.0	0.0	0.0
+32-33	0.22426095820591233	0.0	0.0	0.0	0.0
+34-35	0.24464831804281345	0.0	0.0	0.0	0.0
+36-37	0.3058103975535168	0.0	0.0	0.0	0.0
+38-39	0.4383282364933741	0.0	0.0	0.0	0.0
+40-41	0.4689092762487258	0.0	0.0	0.0	0.0
+42-43	0.4689092762487258	0.0	0.0	0.0	0.0
+44-45	0.4689092762487258	0.0	0.0	0.0	0.0
+46-47	0.4689092762487258	0.0	0.0	0.0	0.0
+48-49	0.4689092762487258	0.0	0.0	0.0	0.0
+50-51	0.4689092762487258	0.0	0.0	0.0	0.0
+52-53	0.4689092762487258	0.0	0.0	0.0	0.0
+54-55	0.4689092762487258	0.0	0.0	0.0	0.0
+56-57	0.4689092762487258	0.0	0.0	0.0	0.0
+58-59	0.4689092762487258	0.0	0.0	0.0	0.0
+60-61	0.4689092762487258	0.0	0.0	0.0	0.0
+62-63	0.4689092762487258	0.0	0.0	0.0	0.0
+64-65	0.4689092762487258	0.0	0.0	0.0	0.0
+66-67	0.4689092762487258	0.0	0.0	0.0	0.0
+68-69	0.4689092762487258	0.0	0.0	0.0	0.0
+70-71	0.4689092762487258	0.0	0.0	0.0	0.0
+72-73	0.4689092762487258	0.0	0.0	0.0	0.0
+74-75	0.4689092762487258	0.0	0.0	0.0	0.0
+76-77	0.4689092762487258	0.0	0.0	0.0	0.0
+78-79	0.4689092762487258	0.0	0.0	0.0	0.0
+80-81	0.4689092762487258	0.0	0.0	0.0	0.0
+82-83	0.4689092762487258	0.0	0.0	0.0	0.0
+84-85	0.4689092762487258	0.0	0.0	0.0	0.0
+86-87	0.4689092762487258	0.0	0.0	0.0	0.0
+88-89	0.4689092762487258	0.0	0.0	0.0	0.0
+90-91	0.4689092762487258	0.0	0.0	0.0	0.0
+92-93	0.4689092762487258	0.0	0.0	0.0	0.0
+94-95	0.4689092762487258	0.0	0.0	0.0	0.0
+96-97	0.47910295616717635	0.0	0.0	0.0	0.0
 >>END_MODULE