##FastQC	0.11.8
>>Basic Statistics	pass
#Measure	Value
Filename	1000trimmed_fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4905
Sequences flagged as poor quality	0
Sequence length	1-108
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.282772680937818	31.0	27.0	33.0	23.0	34.0
2	28.832925336597306	30.0	27.0	32.0	22.0	33.0
3	28.896953588223266	30.0	27.0	32.0	23.0	34.0
4	29.006785934608267	30.0	27.0	32.0	23.0	34.0
5	28.9377990430622	30.0	27.0	32.0	22.0	34.0
6	29.002938706968934	30.0	27.0	32.0	23.0	34.0
7	28.899830220713074	30.0	27.0	32.0	22.0	34.0
8	28.900706184463942	30.0	27.0	32.0	22.0	33.0
9	28.754602555772145	30.0	27.0	32.0	22.0	34.0
10-11	28.822083022456294	30.0	27.0	32.0	22.5	33.0
12-13	28.74898578710789	30.0	26.5	32.0	22.0	33.0
14-15	28.53370750727077	30.0	26.0	32.0	22.0	33.5
16-17	28.52661127583153	30.0	26.0	32.0	22.0	33.0
18-19	28.68343192244741	30.0	26.0	32.0	22.5	33.0
20-21	28.370130166336985	29.5	26.0	32.0	22.0	33.0
22-23	28.185946266098476	29.0	26.0	32.0	22.0	33.0
24-25	28.13009469533431	29.0	26.0	32.0	21.5	33.0
26-27	28.075768267577	29.0	26.0	32.0	21.5	33.0
28-29	27.828024188665616	29.0	25.0	32.0	21.0	33.0
30-31	27.792826876513317	29.0	25.0	31.5	21.0	33.0
32-33	27.490989073616355	28.0	25.0	31.0	21.0	33.0
34-35	27.357199679261562	28.0	24.5	31.0	21.0	33.0
36-37	27.062158129410527	28.0	24.0	31.0	20.5	33.0
38-39	27.238022216783758	28.0	24.0	31.0	21.0	33.0
40-41	27.028963434226593	28.0	24.0	31.0	20.5	33.0
42-43	26.90862078496675	27.0	24.0	31.0	20.5	33.0
44-45	26.632003369751054	27.0	24.0	30.0	20.0	32.0
46-47	26.83042786373109	27.5	24.0	31.0	20.5	32.0
48-49	26.431681832255798	27.0	23.5	30.0	20.0	32.0
50-51	26.219000735104444	27.0	23.0	30.0	20.0	32.0
52-53	25.925690423864786	26.5	22.5	29.5	19.5	31.5
54-55	27.995241016389798	29.5	25.5	31.5	20.5	33.0
56-57	30.410857897242785	31.5	28.5	33.0	25.5	34.0
58-59	30.4083885209713	31.5	28.5	33.0	25.5	34.0
60-61	30.444812362030905	31.5	29.0	33.0	26.0	34.0
62-63	30.44039735099338	31.5	29.0	33.0	25.5	34.0
64-65	30.441712697003883	31.5	29.0	33.0	25.5	34.0
66-67	30.51141384388807	32.0	29.0	33.0	25.5	34.0
68-69	30.05117820324006	31.0	28.0	33.0	24.5	34.0
70-71	30.276141384388808	31.0	29.0	33.0	25.0	34.0
72-73	30.385493372606774	31.5	29.0	33.0	25.5	34.0
74-75	30.213549337260677	31.0	28.5	33.0	25.5	34.0
76-77	29.678939617083948	31.0	28.0	33.0	24.5	34.0
78-79	30.019145802650957	31.0	28.0	33.0	24.5	34.0
80-81	29.664212076583212	31.0	27.5	33.0	24.0	34.0
82-83	29.653534609720175	31.0	28.0	32.5	24.5	34.0
84-85	29.425625920471283	30.5	27.0	32.0	24.0	34.0
86-87	29.34057437407953	30.0	27.0	32.5	24.0	34.0
88-89	28.785714285714285	30.0	27.0	32.0	22.5	33.5
90-91	28.667525773195877	29.5	26.0	32.0	23.0	33.0
92-93	28.565905743740796	29.0	26.0	32.0	23.0	33.0
94-95	28.271723122238583	29.0	26.0	32.0	22.5	33.0
96-97	27.94955817378498	29.0	25.5	31.0	22.0	33.0
98-99	27.641132075471695	28.0	25.0	31.0	21.5	33.0
100-101	27.614716981132077	28.0	25.0	31.0	21.5	33.0
102-103	27.196226415094337	28.0	24.5	31.0	21.0	32.5
104-105	26.870943396226416	27.5	24.0	31.0	20.0	32.5
106-107	26.367593937711003	27.0	23.5	30.0	20.5	32.0
108	27.5650623885918	28.0	24.0	31.0	22.0	33.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
20	7.0
21	24.0
22	47.0
23	78.0
24	226.0
25	513.0
26	830.0
27	1017.0
28	947.0
29	645.0
30	352.0
31	157.0
32	55.0
33	6.0
34	1.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.223241590214066	33.00713557594291	25.97349643221203	19.79612640163099
2	23.76580987352101	31.415748674010608	27.315381476948186	17.503059975520195
3	21.876916785933346	28.70578613780413	29.46227765283173	19.95501942343079
4	21.55048324079786	28.295290972650626	28.850503804236066	21.303721982315444
5	21.219055543998337	30.205949656750576	28.271271063033076	20.303723736218014
6	22.124265323257767	28.946263643996637	26.364399664147776	22.565071368597817
7	21.943972835314092	29.9660441426146	28.395585738539896	19.69439728353141
8	21.378129681147016	28.825165846351382	27.8621870318853	21.934517440616304
9	20.077972709551656	29.088152479965345	28.654970760233915	22.17890405024908
10-11	21.243237275035884	29.468919068124105	27.680247322512976	21.607596334327038
12-13	20.8276950834461	29.03698691926026	29.18358141632837	20.951736580965267
14-15	20.24001846295869	28.975305792753286	29.471497807523654	21.313177936764365
16-17	19.83872880351002	29.396418830783826	29.621724178821296	21.14312818688486
18-19	20.845552297165202	27.871456500488755	29.973118279569892	21.309872922776147
20-21	20.538172715894866	28.973717146433042	29.111389236545683	21.376720901126408
22-23	20.88082901554404	30.129533678756477	29.300518134715027	19.689119170984455
24-25	20.506974248927037	29.07725321888412	29.05042918454936	21.365343347639485
26-27	20.86603139013453	28.054932735426007	30.339125560538115	20.739910313901344
28-29	19.32600732600733	30.842490842490843	28.776556776556777	21.054945054945055
30-31	20.638820638820636	29.19226044226044	29.49938574938575	20.669533169533167
32-33	19.47989016314004	29.009853012437407	29.38136003876595	22.1288967856566
34-35	19.6674020023757	29.713219073477003	30.205328355676226	20.41405056847107
36-37	20.443482963764197	29.853975121687398	30.61114115738237	19.091400757166035
38-39	20.992661259173424	28.041714947856317	30.088837388953266	20.876786404016993
40-41	19.860197368421055	28.680098684210524	30.32483552631579	21.134868421052634
42-43	20.01321003963012	27.873183619550858	30.823425803610743	21.29018053720828
44-45	18.941371943982908	30.192262046047947	29.954901495371466	20.911464514597675
46-47	20.1888718734048	28.126595201633485	31.470137825421133	20.21439509954058
48-49	19.56159822419534	29.689234184239734	28.77358490566038	21.975582685904552
50-51	20.50656087885261	28.2575526396094	30.759841318278912	20.47604516325908
52-53	17.691256830601095	29.918032786885245	31.864754098360653	20.525956284153004
54-55	17.214912280701753	32.30994152046784	29.53216374269006	20.942982456140353
56-57	19.30856932695844	30.893710923133504	30.893710923133504	18.90400882677455
58-59	20.014716703458426	29.72774098601913	31.42016188373804	18.8373804267844
60-61	17.402501839587934	33.18616629874908	29.506990434142754	19.904341427520237
62-63	18.543046357615893	29.72774098601913	32.2663723325975	19.462840323767477
64-65	16.893632683106365	32.94074346705926	30.180345969819655	19.985277880014724
66-67	18.519882179675996	28.902798232695137	32.80559646539028	19.771723122238587
68-69	17.2680412371134	30.375552282768776	31.66421207658321	20.692194403534607
70-71	17.673048600883654	27.798232695139912	35.34609720176731	19.182621502209134
72-73	16.200294550810014	29.528718703976438	33.87334315169367	20.39764359351988
74-75	18.372606774668633	28.019145802650957	34.5360824742268	19.072164948453608
76-77	16.678939617083945	31.70103092783505	31.73784977908689	19.88217967599411
78-79	18.77761413843888	28.387334315169365	32.62150220913107	20.213549337260677
80-81	16.384388807069218	30.927835051546392	32.9160530191458	19.771723122238587
82-83	17.083946980854197	30.927835051546392	32.54786450662739	19.44035346097202
84-85	16.936671575846834	31.590574374079527	31.443298969072163	20.029455081001473
86-87	17.488954344624446	27.908689248895435	34.20471281296024	20.39764359351988
88-89	16.789396170839467	32.36377025036819	31.075110456553755	19.771723122238587
90-91	19.587628865979383	29.749631811487482	33.5419734904271	17.120765832106038
92-93	17.746686303387335	30.04418262150221	31.33284241531664	20.876288659793815
94-95	18.924889543446245	28.460972017673047	33.39469808541973	19.219440353460975
96-97	16.42120765832106	29.418262150220915	32.47422680412371	21.686303387334316
98-99	18.158090976882924	29.34377330350485	33.1469052945563	19.351230425055927
100-101	17.77358490566038	29.849056603773583	33.28301886792453	19.09433962264151
102-103	18.339622641509436	29.509433962264154	30.90566037735849	21.245283018867926
104-105	18.037735849056606	28.67924528301887	31.77358490566038	21.50943396226415
106-107	17.2782874617737	29.357798165137616	33.52446483180428	19.839449541284402
108	20.053475935828878	26.38146167557932	31.72905525846702	21.836007130124777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	15.5
2	16.5
3	17.0
4	18.0
5	21.5
6	26.5
7	30.0
8	33.5
9	36.0
10	41.0
11	47.0
12	47.5
13	56.0
14	65.5
15	69.0
16	72.5
17	78.0
18	86.5
19	94.5
20	105.5
21	113.0
22	123.0
23	132.5
24	149.5
25	172.5
26	200.0
27	222.0
28	247.0
29	282.0
30	310.5
31	337.0
32	370.0
33	403.5
34	438.0
35	465.0
36	481.5
37	511.0
38	523.5
39	506.0
40	491.0
41	491.5
42	482.0
43	483.0
44	487.5
45	471.5
46	471.0
47	468.5
48	473.5
49	472.0
50	437.5
51	415.5
52	407.5
53	398.0
54	381.0
55	364.0
56	349.0
57	341.0
58	330.5
59	320.0
60	320.5
61	301.0
62	275.0
63	245.0
64	207.0
65	190.5
66	182.0
67	173.0
68	167.0
69	151.5
70	131.5
71	121.0
72	117.5
73	110.5
74	104.0
75	90.5
76	75.0
77	67.5
78	62.5
79	61.5
80	59.5
81	57.0
82	54.5
83	47.0
84	39.5
85	38.0
86	36.5
87	35.5
88	28.0
89	21.0
90	19.5
91	17.0
92	15.5
93	14.5
94	13.5
95	13.5
96	15.0
97	15.5
98	17.0
99	16.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	0.0
108	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	98.0
5-9	250.0
10-14	257.0
15-19	279.0
20-24	321.0
25-29	396.0
30-34	401.0
35-39	433.0
40-44	401.0
45-49	385.0
50-54	320.0
55-59	5.0
60-64	1.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	33.0
100-104	0.0
105-109	1325.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.73598369011212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.44249432170142	98.1855249745158
2	0.47491224447656405	0.9378185524974516
3	0.041296716911005574	0.12232415902140673
4	0.020648358455502787	0.08154943934760449
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.020648358455502787	0.672782874617737
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT	33	0.672782874617737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.12232415902140673	0.0	0.0	0.0	0.0
22-23	0.12232415902140673	0.0	0.0	0.0	0.0
24-25	0.12232415902140673	0.0	0.0	0.0	0.0
26-27	0.1325178389398573	0.0	0.0	0.0	0.0
28-29	0.2038735983690112	0.0	0.0	0.0	0.0
30-31	0.22426095820591233	0.0	0.0	0.0	0.0
32-33	0.22426095820591233	0.0	0.0	0.0	0.0
34-35	0.24464831804281345	0.0	0.0	0.0	0.0
36-37	0.3058103975535168	0.0	0.0	0.0	0.0
38-39	0.4383282364933741	0.0	0.0	0.0	0.0
40-41	0.4689092762487258	0.0	0.0	0.0	0.0
42-43	0.4689092762487258	0.0	0.0	0.0	0.0
44-45	0.4689092762487258	0.0	0.0	0.0	0.0
46-47	0.4689092762487258	0.0	0.0	0.0	0.0
48-49	0.4689092762487258	0.0	0.0	0.0	0.0
50-51	0.4689092762487258	0.0	0.0	0.0	0.0
52-53	0.4689092762487258	0.0	0.0	0.0	0.0
54-55	0.4689092762487258	0.0	0.0	0.0	0.0
56-57	0.4689092762487258	0.0	0.0	0.0	0.0
58-59	0.4689092762487258	0.0	0.0	0.0	0.0
60-61	0.4689092762487258	0.0	0.0	0.0	0.0
62-63	0.4689092762487258	0.0	0.0	0.0	0.0
64-65	0.4689092762487258	0.0	0.0	0.0	0.0
66-67	0.4689092762487258	0.0	0.0	0.0	0.0
68-69	0.4689092762487258	0.0	0.0	0.0	0.0
70-71	0.4689092762487258	0.0	0.0	0.0	0.0
72-73	0.4689092762487258	0.0	0.0	0.0	0.0
74-75	0.4689092762487258	0.0	0.0	0.0	0.0
76-77	0.4689092762487258	0.0	0.0	0.0	0.0
78-79	0.4689092762487258	0.0	0.0	0.0	0.0
80-81	0.4689092762487258	0.0	0.0	0.0	0.0
82-83	0.4689092762487258	0.0	0.0	0.0	0.0
84-85	0.4689092762487258	0.0	0.0	0.0	0.0
86-87	0.4689092762487258	0.0	0.0	0.0	0.0
88-89	0.4689092762487258	0.0	0.0	0.0	0.0
90-91	0.4689092762487258	0.0	0.0	0.0	0.0
92-93	0.4689092762487258	0.0	0.0	0.0	0.0
94-95	0.4689092762487258	0.0	0.0	0.0	0.0
96-97	0.47910295616717635	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACG	80	0.0068203025	21.952587	94-95
TAT	565	1.3306382E-4	9.658028	104-105
>>END_MODULE