# HG changeset patch # User iuc # Date 1686254531 0 # Node ID 5ec9f6bceaee7c629268142c0b7bb8f7ab05b51b # Parent 3d0c7bdf12f577edc818fd4a656f0408e4d9d373 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 9aa395df821f0d3607867c83536ac97f9ffe8b29 diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee rgFastQC.xml --- a/rgFastQC.xml Sun Sep 12 11:20:27 2021 +0000 +++ b/rgFastQC.xml Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,10 @@ - + Read Quality reports fastqc - fastqc + fastqc diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data.txt --- a/test-data/fastqc_data.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,81 +345,81 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.4689092762487258 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 0.0 0.0 0.0 0.0 0.020387359836901122 0.0 +2 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +3 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +4 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +5 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +6 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +7 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +8 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +9 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +10-11 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +12-13 0.0 0.0 0.0 0.0 0.1529051987767584 0.0 +14-15 0.0 0.0 0.0 0.0 0.17329255861365955 0.0 +16-17 0.0 0.0 0.0 0.0 0.21406727828746175 0.0 +18-19 0.0 0.0 0.0 0.0 0.22426095820591233 0.0 +20-21 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +22-23 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +24-25 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +26-27 0.1325178389398573 0.0 0.0 0.0 0.24464831804281345 0.0 +28-29 0.2038735983690112 0.0 0.0 0.0 0.24464831804281345 0.0 +30-31 0.22426095820591233 0.0 0.0 0.0 0.24464831804281345 0.0 +32-33 0.22426095820591233 0.0 0.0 0.0 0.2650356778797146 0.0 +34-35 0.24464831804281345 0.0 0.0 0.0 0.29561671763506625 0.0 +36-37 0.3058103975535168 0.0 0.0 0.0 0.31600407747196735 0.0 +38-39 0.4383282364933741 0.0 0.0 0.0 0.32619775739041795 0.0 +40-41 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +42-43 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +44-45 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +46-47 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +48-49 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +50-51 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +52-53 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +54-55 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +56-57 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +58-59 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +60-61 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +62-63 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +64-65 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +66-67 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +68-69 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +70-71 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +72-73 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +74-75 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +76-77 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +78-79 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +80-81 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +82-83 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +84-85 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +86-87 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +88-89 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +90-91 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +92-93 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +94-95 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +96-97 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_adapters.txt --- a/test-data/fastqc_data_adapters.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_adapters.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,23 +345,23 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_contaminants.txt --- a/test-data/fastqc_data_contaminants.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_contaminants.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,81 +345,81 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 TestContaminant (100% over 24bp) >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.4689092762487258 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 0.0 0.0 0.0 0.0 0.020387359836901122 0.0 +2 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +3 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +4 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +5 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +6 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +7 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +8 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +9 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +10-11 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +12-13 0.0 0.0 0.0 0.0 0.1529051987767584 0.0 +14-15 0.0 0.0 0.0 0.0 0.17329255861365955 0.0 +16-17 0.0 0.0 0.0 0.0 0.21406727828746175 0.0 +18-19 0.0 0.0 0.0 0.0 0.22426095820591233 0.0 +20-21 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +22-23 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +24-25 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +26-27 0.1325178389398573 0.0 0.0 0.0 0.24464831804281345 0.0 +28-29 0.2038735983690112 0.0 0.0 0.0 0.24464831804281345 0.0 +30-31 0.22426095820591233 0.0 0.0 0.0 0.24464831804281345 0.0 +32-33 0.22426095820591233 0.0 0.0 0.0 0.2650356778797146 0.0 +34-35 0.24464831804281345 0.0 0.0 0.0 0.29561671763506625 0.0 +36-37 0.3058103975535168 0.0 0.0 0.0 0.31600407747196735 0.0 +38-39 0.4383282364933741 0.0 0.0 0.0 0.32619775739041795 0.0 +40-41 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +42-43 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +44-45 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +46-47 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +48-49 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +50-51 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +52-53 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +54-55 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +56-57 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +58-59 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +60-61 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +62-63 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +64-65 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +66-67 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +68-69 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +70-71 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +72-73 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +74-75 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +76-77 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +78-79 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +80-81 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +82-83 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +84-85 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +86-87 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +88-89 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +90-91 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +92-93 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +94-95 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +96-97 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_customlimits.txt --- a/test-data/fastqc_data_customlimits.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_customlimits.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,83 +345,83 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.4689092762487258 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 0.0 0.0 0.0 0.0 0.020387359836901122 0.0 +2 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +3 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +4 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +5 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +6 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +7 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +8 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +9 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +10-11 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +12-13 0.0 0.0 0.0 0.0 0.1529051987767584 0.0 +14-15 0.0 0.0 0.0 0.0 0.17329255861365955 0.0 +16-17 0.0 0.0 0.0 0.0 0.21406727828746175 0.0 +18-19 0.0 0.0 0.0 0.0 0.22426095820591233 0.0 +20-21 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +22-23 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +24-25 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +26-27 0.1325178389398573 0.0 0.0 0.0 0.24464831804281345 0.0 +28-29 0.2038735983690112 0.0 0.0 0.0 0.24464831804281345 0.0 +30-31 0.22426095820591233 0.0 0.0 0.0 0.24464831804281345 0.0 +32-33 0.22426095820591233 0.0 0.0 0.0 0.2650356778797146 0.0 +34-35 0.24464831804281345 0.0 0.0 0.0 0.29561671763506625 0.0 +36-37 0.3058103975535168 0.0 0.0 0.0 0.31600407747196735 0.0 +38-39 0.4383282364933741 0.0 0.0 0.0 0.32619775739041795 0.0 +40-41 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +42-43 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +44-45 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +46-47 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +48-49 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +50-51 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +52-53 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +54-55 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +56-57 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +58-59 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +60-61 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +62-63 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +64-65 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +66-67 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +68-69 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +70-71 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +72-73 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +74-75 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +76-77 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +78-79 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +80-81 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +82-83 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +84-85 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +86-87 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +88-89 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +90-91 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +92-93 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +94-95 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +96-97 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_hisat.txt --- a/test-data/fastqc_data_hisat.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_hisat.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename hisat_output_1_bam File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20 +Total Bases 1.4 kbp Sequences flagged as poor quality 0 Sequence length 70 %GC 43 @@ -342,106 +343,106 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 -#Duplication Level Percentage of deduplicated Percentage of total -1 100.0 100.0 -2 0.0 0.0 -3 0.0 0.0 -4 0.0 0.0 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.0 0.0 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 100.0 +2 0.0 +3 0.0 +4 0.0 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.0 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source -GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTC 1 5.0 No Hit -CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCT 1 5.0 No Hit -TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAGCGCCGAGGATGCGTGACCGT 1 5.0 No Hit -CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCA 1 5.0 No Hit -CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAA 1 5.0 No Hit -TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCATTGATTTGGTCATT 1 5.0 No Hit -GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGC 1 5.0 No Hit -CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTATCAAAATATAACGTTGACGAT 1 5.0 No Hit -CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCACGCTCCCAAGCATTAATCTCA 1 5.0 No Hit -CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCTAGAA 1 5.0 No Hit -GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCA 1 5.0 No Hit -TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATGTTGACGGGATGAACATAATA 1 5.0 No Hit -CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCT 1 5.0 No Hit -TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTT 1 5.0 No Hit -GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGACGAAGACTCAAAGCGA 1 5.0 No Hit -TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATTACTACTGCTTGTTTACGAAT 1 5.0 No Hit -TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAG 1 5.0 No Hit -CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAAAT 1 5.0 No Hit -ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTC 1 5.0 No Hit -TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAGAACCAGCTTATCAGAAAAAA 1 5.0 No Hit +CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG 1 5.0 No Hit +TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTA 1 5.0 No Hit +ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAG 1 5.0 No Hit +TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATG 1 5.0 No Hit +CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT 1 5.0 No Hit +TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTC 1 5.0 No Hit +CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTT 1 5.0 No Hit +TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAG 1 5.0 No Hit +CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAG 1 5.0 No Hit +GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAG 1 5.0 No Hit +CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTAT 1 5.0 No Hit +GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAA 1 5.0 No Hit +GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAG 1 5.0 No Hit +CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCAC 1 5.0 No Hit +TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACC 1 5.0 No Hit +CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATA 1 5.0 No Hit +TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAG 1 5.0 No Hit +GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGA 1 5.0 No Hit +TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATT 1 5.0 No Hit +CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAA 1 5.0 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10 0.0 0.0 0.0 0.0 0.0 -11 0.0 0.0 0.0 0.0 0.0 -12 0.0 0.0 0.0 0.0 0.0 -13 0.0 0.0 0.0 0.0 0.0 -14 0.0 0.0 0.0 0.0 0.0 -15 0.0 0.0 0.0 0.0 0.0 -16 0.0 0.0 0.0 0.0 0.0 -17 0.0 0.0 0.0 0.0 0.0 -18 0.0 0.0 0.0 0.0 0.0 -19 0.0 0.0 0.0 0.0 0.0 -20 0.0 0.0 0.0 0.0 0.0 -21 0.0 0.0 0.0 0.0 0.0 -22 0.0 0.0 0.0 0.0 0.0 -23 0.0 0.0 0.0 0.0 0.0 -24 0.0 0.0 0.0 0.0 0.0 -25 0.0 0.0 0.0 0.0 0.0 -26 0.0 0.0 0.0 0.0 0.0 -27 0.0 0.0 0.0 0.0 0.0 -28 0.0 0.0 0.0 0.0 0.0 -29 0.0 0.0 0.0 0.0 0.0 -30 0.0 0.0 0.0 0.0 0.0 -31 0.0 0.0 0.0 0.0 0.0 -32 0.0 0.0 0.0 0.0 0.0 -33 0.0 0.0 0.0 0.0 0.0 -34 0.0 0.0 0.0 0.0 0.0 -35 0.0 0.0 0.0 0.0 0.0 -36 0.0 0.0 0.0 0.0 0.0 -37 0.0 0.0 0.0 0.0 0.0 -38 0.0 0.0 0.0 0.0 0.0 -39 0.0 0.0 0.0 0.0 0.0 -40 0.0 0.0 0.0 0.0 0.0 -41 0.0 0.0 0.0 0.0 0.0 -42 0.0 0.0 0.0 0.0 0.0 -43 0.0 0.0 0.0 0.0 0.0 -44 0.0 0.0 0.0 0.0 0.0 -45 0.0 0.0 0.0 0.0 0.0 -46 0.0 0.0 0.0 0.0 0.0 -47 0.0 0.0 0.0 0.0 0.0 -48 0.0 0.0 0.0 0.0 0.0 -49 0.0 0.0 0.0 0.0 0.0 -50 0.0 0.0 0.0 0.0 0.0 -51 0.0 0.0 0.0 0.0 0.0 -52 0.0 0.0 0.0 0.0 0.0 -53 0.0 0.0 0.0 0.0 0.0 -54 0.0 0.0 0.0 0.0 0.0 -55 0.0 0.0 0.0 0.0 0.0 -56 0.0 0.0 0.0 0.0 0.0 -57 0.0 0.0 0.0 0.0 0.0 -58 0.0 0.0 0.0 0.0 0.0 -59 0.0 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 0.0 0.0 0.0 0.0 0.0 0.0 +2 0.0 0.0 0.0 0.0 0.0 0.0 +3 0.0 0.0 0.0 0.0 0.0 0.0 +4 0.0 0.0 0.0 0.0 0.0 0.0 +5 0.0 0.0 0.0 0.0 0.0 0.0 +6 0.0 0.0 0.0 0.0 0.0 0.0 +7 0.0 0.0 0.0 0.0 0.0 0.0 +8 0.0 0.0 0.0 0.0 0.0 0.0 +9 0.0 0.0 0.0 0.0 0.0 0.0 +10 0.0 0.0 0.0 0.0 0.0 0.0 +11 0.0 0.0 0.0 0.0 0.0 0.0 +12 0.0 0.0 0.0 0.0 0.0 0.0 +13 0.0 0.0 0.0 0.0 0.0 0.0 +14 0.0 0.0 0.0 0.0 0.0 0.0 +15 0.0 0.0 0.0 0.0 0.0 0.0 +16 0.0 0.0 0.0 0.0 0.0 0.0 +17 0.0 0.0 0.0 0.0 0.0 0.0 +18 0.0 0.0 0.0 0.0 0.0 0.0 +19 0.0 0.0 0.0 0.0 0.0 0.0 +20 0.0 0.0 0.0 0.0 0.0 0.0 +21 0.0 0.0 0.0 0.0 0.0 0.0 +22 0.0 0.0 0.0 0.0 0.0 0.0 +23 0.0 0.0 0.0 0.0 0.0 0.0 +24 0.0 0.0 0.0 0.0 0.0 0.0 +25 0.0 0.0 0.0 0.0 0.0 0.0 +26 0.0 0.0 0.0 0.0 0.0 0.0 +27 0.0 0.0 0.0 0.0 0.0 0.0 +28 0.0 0.0 0.0 0.0 0.0 0.0 +29 0.0 0.0 0.0 0.0 0.0 0.0 +30 0.0 0.0 0.0 0.0 0.0 0.0 +31 0.0 0.0 0.0 0.0 0.0 0.0 +32 0.0 0.0 0.0 0.0 0.0 0.0 +33 0.0 0.0 0.0 0.0 0.0 0.0 +34 0.0 0.0 0.0 0.0 0.0 0.0 +35 0.0 0.0 0.0 0.0 0.0 0.0 +36 0.0 0.0 0.0 0.0 0.0 0.0 +37 0.0 0.0 0.0 0.0 0.0 0.0 +38 0.0 0.0 0.0 0.0 0.0 0.0 +39 0.0 0.0 0.0 0.0 0.0 0.0 +40 0.0 0.0 0.0 0.0 0.0 0.0 +41 0.0 0.0 0.0 0.0 0.0 0.0 +42 0.0 0.0 0.0 0.0 0.0 0.0 +43 0.0 0.0 0.0 0.0 0.0 0.0 +44 0.0 0.0 0.0 0.0 0.0 0.0 +45 0.0 0.0 0.0 0.0 0.0 0.0 +46 0.0 0.0 0.0 0.0 0.0 0.0 +47 0.0 0.0 0.0 0.0 0.0 0.0 +48 0.0 0.0 0.0 0.0 0.0 0.0 +49 0.0 0.0 0.0 0.0 0.0 0.0 +50 0.0 0.0 0.0 0.0 0.0 0.0 +51 0.0 0.0 0.0 0.0 0.0 0.0 +52 0.0 0.0 0.0 0.0 0.0 0.0 +53 0.0 0.0 0.0 0.0 0.0 0.0 +54 0.0 0.0 0.0 0.0 0.0 0.0 +55 0.0 0.0 0.0 0.0 0.0 0.0 +56 0.0 0.0 0.0 0.0 0.0 0.0 +57 0.0 0.0 0.0 0.0 0.0 0.0 +58 0.0 0.0 0.0 0.0 0.0 0.0 +59 0.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_kmer.txt --- a/test-data/fastqc_data_kmer.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_kmer.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,83 +345,83 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.4689092762487258 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 0.0 0.0 0.0 0.0 0.020387359836901122 0.0 +2 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +3 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +4 0.0 0.0 0.0 0.0 0.06116207951070336 0.0 +5 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +6 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +7 0.0 0.0 0.0 0.0 0.12232415902140673 0.0 +8 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +9 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +10-11 0.0 0.0 0.0 0.0 0.14271151885830785 0.0 +12-13 0.0 0.0 0.0 0.0 0.1529051987767584 0.0 +14-15 0.0 0.0 0.0 0.0 0.17329255861365955 0.0 +16-17 0.0 0.0 0.0 0.0 0.21406727828746175 0.0 +18-19 0.0 0.0 0.0 0.0 0.22426095820591233 0.0 +20-21 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +22-23 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +24-25 0.12232415902140673 0.0 0.0 0.0 0.24464831804281345 0.0 +26-27 0.1325178389398573 0.0 0.0 0.0 0.24464831804281345 0.0 +28-29 0.2038735983690112 0.0 0.0 0.0 0.24464831804281345 0.0 +30-31 0.22426095820591233 0.0 0.0 0.0 0.24464831804281345 0.0 +32-33 0.22426095820591233 0.0 0.0 0.0 0.2650356778797146 0.0 +34-35 0.24464831804281345 0.0 0.0 0.0 0.29561671763506625 0.0 +36-37 0.3058103975535168 0.0 0.0 0.0 0.31600407747196735 0.0 +38-39 0.4383282364933741 0.0 0.0 0.0 0.32619775739041795 0.0 +40-41 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +42-43 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +44-45 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +46-47 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +48-49 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +50-51 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +52-53 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +54-55 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +56-57 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +58-59 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +60-61 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +62-63 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +64-65 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +66-67 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +68-69 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +70-71 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +72-73 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +74-75 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +76-77 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +78-79 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +80-81 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +82-83 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +84-85 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +86-87 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +88-89 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +90-91 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +92-93 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +94-95 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +96-97 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_min_length.txt --- a/test-data/fastqc_data_min_length.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_min_length.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -344,87 +345,87 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 -10-11 0.0 0.0 0.0 0.0 0.0 -12-13 0.0 0.0 0.0 0.0 0.0 -14-15 0.0 0.0 0.0 0.0 0.0 -16-17 0.0 0.0 0.0 0.0 0.0 -18-19 0.0 0.0 0.0 0.0 0.0 -20-21 0.12232415902140673 0.0 0.0 0.0 0.0 -22-23 0.12232415902140673 0.0 0.0 0.0 0.0 -24-25 0.12232415902140673 0.0 0.0 0.0 0.0 -26-27 0.1325178389398573 0.0 0.0 0.0 0.0 -28-29 0.2038735983690112 0.0 0.0 0.0 0.0 -30-31 0.22426095820591233 0.0 0.0 0.0 0.0 -32-33 0.22426095820591233 0.0 0.0 0.0 0.0 -34-35 0.24464831804281345 0.0 0.0 0.0 0.0 -36-37 0.3058103975535168 0.0 0.0 0.0 0.0 -38-39 0.4383282364933741 0.0 0.0 0.0 0.0 -40-41 0.4689092762487258 0.0 0.0 0.0 0.0 -42-43 0.4689092762487258 0.0 0.0 0.0 0.0 -44-45 0.4689092762487258 0.0 0.0 0.0 0.0 -46-47 0.4689092762487258 0.0 0.0 0.0 0.0 -48-49 0.4689092762487258 0.0 0.0 0.0 0.0 -50-51 0.4689092762487258 0.0 0.0 0.0 0.0 -52-53 0.4689092762487258 0.0 0.0 0.0 0.0 -54-55 0.4689092762487258 0.0 0.0 0.0 0.0 -56-57 0.4689092762487258 0.0 0.0 0.0 0.0 -58-59 0.4689092762487258 0.0 0.0 0.0 0.0 -60-61 0.4689092762487258 0.0 0.0 0.0 0.0 -62-63 0.4689092762487258 0.0 0.0 0.0 0.0 -64-65 0.4689092762487258 0.0 0.0 0.0 0.0 -66-67 0.4689092762487258 0.0 0.0 0.0 0.0 -68-69 0.4689092762487258 0.0 0.0 0.0 0.0 -70-71 0.4689092762487258 0.0 0.0 0.0 0.0 -72-73 0.4689092762487258 0.0 0.0 0.0 0.0 -74-75 0.4689092762487258 0.0 0.0 0.0 0.0 -76-77 0.4689092762487258 0.0 0.0 0.0 0.0 -78-79 0.4689092762487258 0.0 0.0 0.0 0.0 -80-81 0.4689092762487258 0.0 0.0 0.0 0.0 -82-83 0.4689092762487258 0.0 0.0 0.0 0.0 -84-85 0.4689092762487258 0.0 0.0 0.0 0.0 -86-87 0.4689092762487258 0.0 0.0 0.0 0.0 -88-89 0.4689092762487258 0.0 0.0 0.0 0.0 -90-91 0.4689092762487258 0.0 0.0 0.0 0.0 -92-93 0.4689092762487258 0.0 0.0 0.0 0.0 -94-95 0.4689092762487258 0.0 0.0 0.0 0.0 -96-97 0.4689092762487258 0.0 0.0 0.0 0.0 -98-99 0.0 0.0 0.0 0.0 0.0 -100-101 0.0 0.0 0.0 0.0 0.0 -102-103 0.0 0.0 0.0 0.0 0.0 -104-105 0.0 0.0 0.0 0.0 0.0 -106-107 0.0 0.0 0.0 0.0 0.0 -108 0.0 0.0 0.0 0.0 0.0 +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG +1 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0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +98-99 0.0 0.0 0.0 0.0 0.0 0.0 +100-101 0.0 0.0 0.0 0.0 0.0 0.0 +102-103 0.0 0.0 0.0 0.0 0.0 0.0 +104-105 0.0 0.0 0.0 0.0 0.0 0.0 +106-107 0.0 0.0 0.0 0.0 0.0 0.0 +108 0.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_data_nogroup.txt --- a/test-data/fastqc_data_nogroup.txt Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_data_nogroup.txt Thu Jun 08 20:02:11 2023 +0000 @@ -1,10 +1,11 @@ -##FastQC 0.11.9 +##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 +Total Bases 254.2 kbp Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 @@ -577,125 +578,125 @@ >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.44249432170142 98.1855249745158 -2 0.47491224447656405 0.9378185524974516 -3 0.041296716911005574 0.12232415902140673 -4 0.020648358455502787 0.08154943934760449 -5 0.0 0.0 -6 0.0 0.0 -7 0.0 0.0 -8 0.0 0.0 -9 0.0 0.0 ->10 0.020648358455502787 0.672782874617737 ->50 0.0 0.0 ->100 0.0 0.0 ->500 0.0 0.0 ->1k 0.0 0.0 ->5k 0.0 0.0 ->10k+ 0.0 0.0 +#Duplication Level Percentage of total +1 98.1855249745158 +2 0.9378185524974516 +3 0.12232415902140673 +4 0.08154943934760449 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +>10 0.672782874617737 +>50 0.0 +>100 0.0 +>500 0.0 +>1k 0.0 +>5k 0.0 +>10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter -1 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 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0.32619775739041795 0.0 +81 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +82 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +83 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +84 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +85 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +86 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +87 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +88 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +89 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +90 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +91 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +92 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +93 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +94 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +95 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +96 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 +97 0.4689092762487258 0.0 0.0 0.0 0.32619775739041795 0.0 >>END_MODULE diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report.html --- a/test-data/fastqc_report.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_adapters.html --- a/test-data/fastqc_report_adapters.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_adapters.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_contaminants.html --- a/test-data/fastqc_report_contaminants.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_contaminants.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737TestContaminant (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737TestContaminant (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_customlimits.html --- a/test-data/fastqc_report_customlimits.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_customlimits.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_hisat.html --- a/test-data/fastqc_report_hisat.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_hisat.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -hisat_output_1_bam FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
hisat_output_1_bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamehisat_output_1_bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length70
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTC15.0No Hit
CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCT15.0No Hit
TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAGCGCCGAGGATGCGTGACCGT15.0No Hit
CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCA15.0No Hit
CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAA15.0No Hit
TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCATTGATTTGGTCATT15.0No Hit
GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGC15.0No Hit
CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTATCAAAATATAACGTTGACGAT15.0No Hit
CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCACGCTCCCAAGCATTAATCTCA15.0No Hit
CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCTAGAA15.0No Hit
GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCA15.0No Hit
TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATGTTGACGGGATGAACATAATA15.0No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCT15.0No Hit
TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTT15.0No Hit
GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGACGAAGACTCAAAGCGA15.0No Hit
TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATTACTACTGCTTGTTTACGAAT15.0No Hit
TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAG15.0No Hit
CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAAAT15.0No Hit
ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTC15.0No Hit
TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAGAACCAGCTTATCAGAAAAAA15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
\ No newline at end of file +hisat_output_1_bam FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
hisat_output_1_bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamehisat_output_1_bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Total Bases1.4 kbp
Sequences flagged as poor quality0
Sequence length70
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG15.0No Hit
TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTA15.0No Hit
ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAG15.0No Hit
TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATG15.0No Hit
CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT15.0No Hit
TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTC15.0No Hit
CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTT15.0No Hit
TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAG15.0No Hit
CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAG15.0No Hit
GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAG15.0No Hit
CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTAT15.0No Hit
GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAA15.0No Hit
GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAG15.0No Hit
CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCAC15.0No Hit
TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACC15.0No Hit
CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATA15.0No Hit
TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAG15.0No Hit
GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGA15.0No Hit
TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATT15.0No Hit
CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAA15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_kmer.html --- a/test-data/fastqc_report_kmer.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_kmer.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACG800.006820302521.95258794-95
TAT5651.3306382E-49.658028104-105
Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACG800.006820302521.95258794-95
TAT5651.3306382E-49.658028104-105
Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_min_length.html --- a/test-data/fastqc_report_min_length.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_min_length.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
\ No newline at end of file +1000trimmed_fastq FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r 3d0c7bdf12f5 -r 5ec9f6bceaee test-data/fastqc_report_nogroup.html --- a/test-data/fastqc_report_nogroup.html Sun Sep 12 11:20:27 2021 +0000 +++ b/test-data/fastqc_report_nogroup.html Thu Jun 08 20:02:11 2023 +0000 @@ -1,187 +1,187 @@ -1000trimmed_fastq FastQC Report
FastQCFastQC Report
Fri 10 Sep 2021
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)
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FastQCFastQC Report
Thu 8 Jun 2023
1000trimmed_fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Total Bases254.2 kbp
Sequences flagged as poor quality0
Sequence length1-108
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672782874617737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
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