Mercurial > repos > devteam > fastqsolexa_to_fasta_qual

comparison fastqsolexa_to_fasta_qual.py @ 0:ef23c03d7497 draft default tip

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Imported from capsule None
author devteam
date Mon, 19 May 2014 12:33:24 -0400
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-1:000000000000 0:ef23c03d7497
1 #!/usr/bin/env python
2
3 """
4 convert fastqsolexa file to separated sequence and quality files.
5
6 assume each sequence and quality score are contained in one line
7 the order should be:
8 1st line: @title_of_seq
9 2nd line: nucleotides
10 3rd line: +title_of_qualityscore (might be skipped)
11 4th line: quality scores
12 (in three forms: a. digits, b. ASCII codes, the first char as the coding base, c. ASCII codes without the first char.)
13
14 Usage:
15 %python fastqsolexa_to_fasta_qual.py <your_fastqsolexa_filename> <output_seq_filename> <output_score_filename>
16 """
17
18 import sys, os
19 from math import *
20
21 assert sys.version_info[:2] >= ( 2, 4 )
22
23 def stop_err( msg ):
24 sys.stderr.write( "%s" % msg )
25 sys.exit()
26
27 def __main__():
28 infile_name = sys.argv[1]
29 outfile_seq = open( sys.argv[2], 'w' )
30 outfile_score = open( sys.argv[3], 'w' )
31 datatype = sys.argv[4]
32 seq_title_startswith = ''
33 qual_title_startswith = ''
34 default_coding_value = 64
35 fastq_block_lines = 0
36
37 for i, line in enumerate( file( infile_name ) ):
38 line = line.rstrip()
39 if not line or line.startswith( '#' ):
40 continue
41 fastq_block_lines = ( fastq_block_lines + 1 ) % 4
42 line_startswith = line[0:1]
43 if fastq_block_lines == 1:
44 # first line is @title_of_seq
45 if not seq_title_startswith:
46 seq_title_startswith = line_startswith
47 if line_startswith != seq_title_startswith:
48 outfile_seq.close()
49 outfile_score.close()
50 stop_err( 'Invalid fastqsolexa format at line %d: %s.' % ( i + 1, line ) )
51 read_title = line[1:]
52 outfile_seq.write( '>%s\n' % line[1:] )
53 elif fastq_block_lines == 2:
54 # second line is nucleotides
55 read_length = len( line )
56 outfile_seq.write( '%s\n' % line )
57 elif fastq_block_lines == 3:
58 # third line is +title_of_qualityscore ( might be skipped )
59 if not qual_title_startswith:
60 qual_title_startswith = line_startswith
61 if line_startswith != qual_title_startswith:
62 outfile_seq.close()
63 outfile_score.close()
64 stop_err( 'Invalid fastqsolexa format at line %d: %s.' % ( i + 1, line ) )
65 quality_title = line[1:]
66 if quality_title and read_title != quality_title:
67 outfile_seq.close()
68 outfile_score.close()
69 stop_err( 'Invalid fastqsolexa format at line %d: sequence title "%s" differes from score title "%s".' % ( i + 1, read_title, quality_title ) )
70 if not quality_title:
71 outfile_score.write( '>%s\n' % read_title )
72 else:
73 outfile_score.write( '>%s\n' % line[1:] )
74 else:
75 # fourth line is quality scores
76 qual = ''
77 fastq_integer = True
78 # peek: ascii or digits?
79 val = line.split()[0]
80 try:
81 check = int( val )
82 fastq_integer = True
83 except:
84 fastq_integer = False
85
86 if fastq_integer:
87 # digits
88 qual = line
89 else:
90 # ascii
91 quality_score_length = len( line )
92 if quality_score_length == read_length + 1:
93 # first char is qual_score_startswith
94 qual_score_startswith = ord( line[0:1] )
95 line = line[1:]
96 elif quality_score_length == read_length:
97 qual_score_startswith = default_coding_value
98 else:
99 stop_err( 'Invalid fastqsolexa format at line %d: the number of quality scores ( %d ) is not the same as bases ( %d ).' % ( i + 1, quality_score_length, read_length ) )
100 for j, char in enumerate( line ):
101 score = ord( char ) - qual_score_startswith # 64
102 qual = "%s%s " % ( qual, str( score ) )
103 outfile_score.write( '%s\n' % qual )
104
105 outfile_seq.close()
106 outfile_score.close()
107
108 if __name__ == "__main__": __main__()
109