# HG changeset patch
# User devteam
# Date 1380122064 14400
# Node ID bcc5424a4a3c70fb0ef6c81962cee580683dfe03
Uploaded tool tarball.
diff -r 000000000000 -r bcc5424a4a3c fastx_nucleotides_distribution.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/fastx_nucleotides_distribution.xml Wed Sep 25 11:14:24 2013 -0400
@@ -0,0 +1,53 @@
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+ fastx_toolkit
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+ fastx_nucleotide_distribution_graph.sh -t '$input.name' -i $input -o $output
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+**What it does**
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+Creates a stacked-histogram graph for the nucleotide distribution in the Solexa library.
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+.. class:: infomark
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+**TIP:** Use the **FASTQ Statistics** tool to generate the report file needed for this tool.
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+-----
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+**Output Examples**
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+The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT**
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+.. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_1.png
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+In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...**
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+.. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_2.png
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+The following chart shows a growing number of unknown (N) nucleotides towards later cycles (which might indicate a sequencing problem):
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+.. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_3.png
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+But most of the time, the chart will look rather random:
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+.. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_4.png
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+------
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+This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
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+ .. __: http://hannonlab.cshl.edu/fastx_toolkit/
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diff -r 000000000000 -r bcc5424a4a3c tool_dependencies.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml Wed Sep 25 11:14:24 2013 -0400
@@ -0,0 +1,6 @@
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