annotate fastx_reverse_complement.xml @ 3:3f1017d3f785 draft

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author iuc
date Tue, 08 May 2018 13:28:22 -0400
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1 <tool id="cshl_fastx_reverse_complement" version="1.0.1" name="Reverse-Complement">
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="exit_code"><![CDATA[
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8 @CATS@ fastx_reverse_complement -v
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9 -o '$output'
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10 @FQQUAL@
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11 ]]></command>
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12 <inputs>
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13 <expand macro="fastx_input" />
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14 </inputs>
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15 <outputs>
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16 <data name="output" format_source="input" metadata_source="input" />
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17 </outputs>
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18 <tests>
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19 <test>
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20 <!-- Reverse-complement a FASTA file -->
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21 <param name="input" value="fastx_rev_comp1.fasta" />
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22 <output name="output" ftype="fasta" file="fastx_reverse_complement1.out" />
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23 </test>
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24 <test>
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25 <!-- Reverse-complement a FASTQ file -->
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26 <param name="input" value="fastx_rev_comp2.fastq" ftype="fastqsolexa"/>
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27 <output name="output" ftype="fastqsolexa" file="fastx_reverse_complement2.out" />
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28 </test>
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29 </tests>
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30 <help><![CDATA[
0
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31 **What it does**
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32
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33 This tool reverse-complements each sequence in a library.
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34 If the library is a FASTQ, the quality-scores are also reversed.
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35
0
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36 --------
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37
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38 **Example**
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39
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40 Input FASTQ file::
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41
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42 @CSHL_1_FC42AGWWWXX:8:1:3:740
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43 TGTCTGTAGCCTCNTCCTTGTAATTCAAAGNNGGTA
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44 +CSHL_1_FC42AGWWWXX:8:1:3:740
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45 33 33 33 34 33 33 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 27 21 27 33 32 31 29 26 24 5 5 15 17 27 26
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46
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47
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48 Output FASTQ file::
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49
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50 @CSHL_1_FC42AGWWWXX:8:1:3:740
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51 TACCNNCTTTGAATTACAAGGANGAGGCTACAGACA
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52 +CSHL_1_FC42AGWWWXX:8:1:3:740
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53 26 27 17 15 5 5 24 26 29 31 32 33 27 21 27 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 33 33 34 33 33 33
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54
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55 ------
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56
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57 This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
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58
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59 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
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60 ]]></help>
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61 <expand macro="citations" />
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62 </tool>