Mercurial > repos > devteam > featurecounter
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author | devteam |
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date | Tue, 01 Apr 2014 10:51:34 -0400 |
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<tool id="featureCoverage1" name="Feature coverage" version="2.0.0"> <description></description> <requirements> <requirement type="package" version="0.7.1">bx-python</requirement> <requirement type="package" version="1.0.0">galaxy-ops</requirement> </requirements> <command interpreter="python">featureCounter.py $input1 $input2 $output -1 ${input1.metadata.chromCol},${input1.metadata.startCol},${input1.metadata.endCol},${input1.metadata.strandCol} -2 ${input2.metadata.chromCol},${input2.metadata.startCol},${input2.metadata.endCol},${input2.metadata.strandCol}</command> <inputs> <param format="interval" name="input1" type="data" help="First dataset"> <label>What portion of</label> </param> <param format="interval" name="input2" type="data" help="Second dataset"> <label>is covered by</label> </param> </inputs> <outputs> <data format="interval" name="output" metadata_source="input1" /> </outputs> <tests> <test> <param name="input1" value="1.bed" /> <param name="input2" value="2.bed" /> <output name="output" file="6_feature_coverage.bed" /> </test> <test> <param name="input1" value="chrY1.bed" /> <param name="input2" value="chrY2.bed" /> <output name="output" file="chrY_Coverage.bed" /> </test> </tests> <help> .. class:: infomark **What it does** This tool finds the coverage of intervals in the first dataset on intervals in the second dataset. The coverage and count are appended as 4 new columns in the resulting dataset. ----- **Example** - If **First dataset** consists of the following windows:: chrX 1 10001 seg 0 - chrX 10001 20001 seg 0 - chrX 20001 30001 seg 0 - chrX 30001 40001 seg 0 - - and **Second dataset** consists of the following exons:: chrX 5000 6000 seg2 0 - chrX 5500 7000 seg2 0 - chrX 9000 22000 seg2 0 - chrX 24000 34000 seg2 0 - chrX 36000 38000 seg2 0 - - the **Result** is the coverage of exons of the second dataset in each of the windows contained in first dataset:: chrX 1 10001 seg 0 - 3001 0.3001 2 1 chrX 10001 20001 seg 0 - 10000 1.0 1 0 chrX 20001 30001 seg 0 - 8000 0.8 0 2 chrX 30001 40001 seg 0 - 5999 0.5999 1 1 - To clarify, the following line of output ( added columns are indexed by a, b and c ):: a b c d chrX 1 10001 seg 0 - 3001 0.3001 2 1 implies that 2 exons (c) fall fully in this window (chrX:1-10001), 1 exon (d) partially overlaps this window, and these 3 exons cover 30.01% (c) of the window size, spanning 3001 nucleotides (a). * a: number of nucleotides in this window covered by the features in (c) and (d) - features overlapping with each other will be merged to calculate (a) * b: fraction of window size covered by features in (c) and (d) - features overlapping with each other will be merged to calculate (b) * c: number of features in the 2nd dataset that fall **completely** within this window * d: number of features in the 2nd dataset that **partially** overlap this window </help> </tool>