Mercurial > repos > devteam > hgv_hilbertvis
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author | devteam |
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date | Wed, 25 Sep 2013 11:23:48 -0400 |
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#!/usr/bin/env bash input_file="$1" output_file="$2" chromInfo_file="$3" chrom="$4" score_col="$5" hilbert_curve_level="$6" summarization_mode="$7" chrom_col="$8" start_col="$9" end_col="${10}" strand_col="${11}" ## use first sequence if chrom filed is empty if [ -z "$chrom" ]; then chrom=$( head -n 1 "$input_file" | cut -f$chrom_col ) fi ## get sequence length if [ ! -r "$chromInfo_file" ]; then echo "Unable to read chromInfo_file $chromInfo_file" 1>&2 exit 1 fi chrom_len=$( awk '$1 == chrom {print $2}' chrom=$chrom $chromInfo_file ) ## error if we can't find the chrom_len if [ -z "$chrom_len" ]; then echo "Can't find length for sequence \"$chrom\" in chromInfo_file $chromInfo_file" 1>&2 exit 1 fi ## make sure chrom_len is positive if [ $chrom_len -le 0 ]; then echo "sequence \"$chrom\" length $chrom_len <= 0" 1>&2 exit 1 fi ## modify R script depending on the inclusion of a score column, strand information input_cols="\$${start_col}, \$${end_col}" col_types='beg=0, end=0, strand=""' # if strand_col == 0 (strandCol metadata is not set), assume everything's on the plus strand if [ $strand_col -ne 0 ]; then input_cols="${input_cols}, \$${strand_col}" else input_cols="${input_cols}, \\\"+\\\"" fi # set plot value (either from data or use a constant value) if [ $score_col -eq -1 ]; then value=1 else input_cols="${input_cols}, \$${score_col}" col_types="${col_types}, score=0" value='chunk$score[i]' fi R --vanilla &> /dev/null <<endOfCode library(HilbertVis); chrom_len <- ${chrom_len}; chunk_size <- 1000; interval_count <- 0; invalid_strand <- 0; awk_cmd <- paste( "awk 'BEGIN{FS=\"\t\";OFS=\"\t\"}", "\$${chrom_col} == \"${chrom}\"", "{print ${input_cols}}' ${input_file}" ); col_types <- list(${col_types}); vec <- vector(mode="numeric", length=chrom_len); conn <- pipe(description=awk_cmd, open="r"); repeat { chunk <- scan(file=conn, what=col_types, sep="\t", nlines=chunk_size, quiet=TRUE); if ((rows <- length(chunk\$beg)) == 0) break; interval_count <- interval_count + rows; for (i in 1:rows) { if (chunk\$strand[i] == '+') { start <- chunk\$beg[i] + 1; stop <- chunk\$end[i]; } else if (chunk\$strand[i] == '-') { start <- chrom_len - chunk\$end[i] - 1; stop <- chrom_len - chunk\$beg[i]; } else { invalid_strand <- invalid_strand + 1; interval_count <- interval_count - 1; next; } vec[start:stop] <- ${value}; } } close(conn); hMat <- hilbertImage(vec, level=$hilbert_curve_level, mode="$summarization_mode"); pdf(file="$output_file", onefile=TRUE, width=8, height=10.5, paper="letter"); showHilbertImage(hMat); dev.off(); endOfCode