hisat: hisat.xml annotate

annotate hisat.xml @ 3:0505bdb7d6b1 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit a1517c9d22029095120643bbe2c8fa53754dd2b7

author	devteam
date	Wed, 11 Nov 2015 12:17:00 -0500
parents	4d91675e16e8
children

rev	line source
2 4d91675e16e8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 00a7926c285bc4a339bd7deebf40b28f39c7d947 devteam parents: 1 diff changeset	1 <tool id="hisat" name="HISAT" version="1.0.3">
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	2 <description>spliced aligner</description>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	3 <macros>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	4 <import>hisat_macros.xml</import>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	5 </macros>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	6 <requirements>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	7 <requirement type="package" version="0.1.6">hisat</requirement>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	8 <requirement type="package" version="1.2">samtools</requirement>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	9 </requirements>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	10 <stdio>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	11 <exit_code range="1:" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	12 </stdio>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	13 <version_command>hisat --version</version_command>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	14 <command><![CDATA[
1 8bb3efa0dae8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 8000b1dd0d6b336707cf84867d11f690f6a26f5c devteam parents: 0 diff changeset	15 #if str($spliced_options.spliced_options_selector) == "advanced" and str($spliced_options.known_splice_gtf) != 'None':
8bb3efa0dae8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 8000b1dd0d6b336707cf84867d11f690f6a26f5c devteam parents: 0 diff changeset	16 ln -s "${spliced_options.known_splice_gtf}" splice_sites.gtf &&
8bb3efa0dae8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 8000b1dd0d6b336707cf84867d11f690f6a26f5c devteam parents: 0 diff changeset	17 extract_splice_sites.py splice_sites.gtf > splice_sites.txt &&
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	18 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	19 #if $reference_genome.reference_genome_source == "history":
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	20 ln -s "$reference_genome.history_item" genome.fa &&
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	21 hisat-build genome.fa genome &&
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	22 #set index_path = 'genome'
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	23 #else:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	24 #set index_path = $reference_genome.index.fields.path
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	25 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	26 hisat -p \${GALAXY_SLOTS:-1} -x "${index_path}"
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	27 #if str($input_format.paired.paired_selector) == 'paired':
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	28 -1 "${reads_f}" -2 "${reads_r}"
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	29 #else if str($input_format.paired.paired_selector) == 'paired_collection':
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	30 -1 "${input_format.paired.reads.forward}" -2 "${input_format.paired.reads.reverse}"
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	31 #else:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	32 -U "${reads}"
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	33 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	34 #if $input_format.input_format_selector == 'fasta':
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	35 -f
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	36 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	37 #if str($input_options.input_options_selector) == "advanced":
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	38 -s ${input_options.skip} -u ${input_options.stop_after} -5 ${input_options.trim_five} -3 ${input_options.trim_three}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	39 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	40 #if str($scoring_options.scoring_options_selector) == "advanced":
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	41 --ma ${scoring_options.match_bonus} --mp ${scoring_options.max_mismatch},${scoring_options.min_mismatch}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	42 --np ${scoring_options.ambiguous_penalty} --rdg ${scoring_options.read_open_penalty},${scoring_options.read_extend_penalty}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	43 --rfg ${scoring_options.ref_open_penalty},${scoring_options.ref_extend_penalty}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	44 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	45 #if str($alignment_options.alignment_options_selector) == "advanced":
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	46 --n-ceil ${alignment_options.function_type},${alignment_options.constant_term},${alignment_options.coefficient_term}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	47 ${alignment_options.skip_forward} ${alignment_options.skip_reverse}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	48 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	49 #if str($spliced_options.spliced_options_selector) == "advanced":
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	50 --pen-cansplice ${spliced_options.canonical_penalty} --pen-noncansplice ${spliced_options.noncanonical_penalty}
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	51 --pen-intronlen ${spliced_options.function_type},${spliced_options.constant_term},${spliced_options.coefficient_term}
1 8bb3efa0dae8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 8000b1dd0d6b336707cf84867d11f690f6a26f5c devteam parents: 0 diff changeset	52 #if str($spliced_options.known_splice_gtf) != 'None':
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	53 --known-splicesite-infile splice_sites.txt
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	54 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	55 #end if
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	56 \| samtools view -bS - \| samtools sort - -o hsbam > "${output_alignments}"
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	57 ]]></command>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	58 <inputs>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	59 <conditional name="input_format">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	60 <param name="input_format_selector" label="Input data format" type="select">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	61 <option value="fastq" selected="selected">FASTQ</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	62 <option value="fasta">FASTA</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	63 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	64 <when value="fasta">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	65 <conditional name="paired">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	66 <expand macro="single_paired_selector" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	67 <when value="paired_collection">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	68 <param format="fasta" name="reads" type="data_collection" collection_type="paired" label="Paired reads" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	69 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	70 <when value="paired_list">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	71 <param format="fasta" name="reads" type="data_collection" collection_type="list:paired" label="Paired reads" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	72 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	73 <when value="paired">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	74 <param label="Forward reads" type="data" name="reads_f" multiple="true" format="fasta" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	75 <param label="Reverse reads" type="data" name="reads_r" multiple="true" format="fasta" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	76 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	77 <when value="single">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	78 <param label="Reads" type="data" name="reads" multiple="true" format="fasta" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	79 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	80 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	81 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	82 <when value="fastq">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	83 <conditional name="paired">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	84 <expand macro="single_paired_selector" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	85 <when value="paired_collection">
2 4d91675e16e8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 00a7926c285bc4a339bd7deebf40b28f39c7d947 devteam parents: 1 diff changeset	86 <param format="fastq" name="reads" type="data_collection" collection_type="paired" label="Paired reads" />
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	87 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	88 <when value="paired">
2 4d91675e16e8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 00a7926c285bc4a339bd7deebf40b28f39c7d947 devteam parents: 1 diff changeset	89 <param label="Forward reads" type="data" name="reads_f" multiple="true" format="fastq" />
4d91675e16e8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 00a7926c285bc4a339bd7deebf40b28f39c7d947 devteam parents: 1 diff changeset	90 <param label="Reverse reads" type="data" name="reads_r" multiple="true" format="fastq" />
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	91 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	92 <when value="single">
2 4d91675e16e8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 00a7926c285bc4a339bd7deebf40b28f39c7d947 devteam parents: 1 diff changeset	93 <param label="Reads" type="data" name="reads" multiple="true" format="fastq" />
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	94 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	95 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	96 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	97 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	98 <conditional name="reference_genome">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	99 <param name="reference_genome_source" type="select" label="Source for the reference genome to align against" help="Built-in references were created using default options">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	100 <option value="indexed" selected="True">Use a built-in genome</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	101 <option value="history">Use a genome from history</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	102 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	103 <when value="indexed">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	104 <param name="index" type="select" label="Select a reference genome" help="If your genome of interest is not listed, contact the Galaxy team">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	105 <options from_data_table="hisat_indexes">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	106 <filter type="sort_by" column="2"/>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	107 <validator type="no_options" message="No genomes are available for the selected input dataset"/>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	108 </options>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	109 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	110 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	111 <when value="history">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	112 <param name="history_item" type="data" format="fasta" metadata_name="dbkey" label="Select the reference genome" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	113 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	114 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	115 <conditional name="alignment_options">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	116 <param label="Alignment options" name="alignment_options_selector" type="select">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	117 <option value="defaults">Use default values</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	118 <option value="advanced">Specify alignment parameters</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	119 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	120 <when value="defaults" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	121 <when value="advanced">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	122 <expand macro="function">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	123 <label>Sets a function governing the maximum number of ambiguous characters</label>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	124 </expand>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	125 <param name="ignore_quals" label="Ignore quality values" type="boolean" truevalue="--ignore-quals" falsevalue="" help="(--ignore-quals) When calculating a mismatch penalty, always consider the quality value at the mismatched position to be the highest possible, regardless of the actual value. I.e. input is treated as though all quality values are high. This is also the default behavior when the input doesn't specify quality values." />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	126 <param name="skip_forward" label="Skip forward strand of reference" type="boolean" truevalue="--nofw" falsevalue="" help="(--nofw) If --nofw is specified, hisat will not attempt to align unpaired reads to the forward (Watson) reference strand. In paired-end mode, --nofw and --norc pertain to the fragments; i.e. specifying --nofw causes hisat to explore only those paired-end configurations corresponding to fragments from the reverse-complement (Crick) strand." />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	127 <param name="skip_reverse" label="Skip reference strand of reference" type="boolean" truevalue="--norc" falsevalue="" help="(--norc) If --norc is specified, hisat will not attempt to align unpaired reads against the reverse-complement (Crick) reference strand. In paired-end mode, --nofw and --norc pertain to the fragments; i.e. specifying --nofw causes hisat to explore only those paired-end configurations corresponding to fragments from the reverse-complement (Crick) strand." />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	128 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	129 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	130 <conditional name="input_options">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	131 <param label="Input options" name="input_options_selector" type="select">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	132 <option value="defaults">Use default values</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	133 <option value="advanced">Specify input parameters</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	134 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	135 <when value="defaults" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	136 <when value="advanced">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	137 <param name="skip" label="Skip the first N reads or pairs in the input" help="-s" type="integer" value="0" default="0" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	138 <param name="stop_after" label="Stop after aligning N reads" help="(-u) Align the first N reads or read pairs from the input (after the first N reads or pairs have been skipped), then stop." type="integer" value="0" default="0" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	139 <param name="trim_five" label="Trim 5' end" help="(-5) Trim N bases from 5' (left) end of each read before alignment" type="integer" value="0" default="0" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	140 <param name="trim_three" label="Trim 3' end" help="(-3) Trim N bases from 3' (right) end of each read before alignment" type="integer" value="0" default="0" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	141 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	142 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	143 <conditional name="scoring_options">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	144 <param label="Scoring options" name="scoring_options_selector" type="select">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	145 <option value="defaults">Use default values</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	146 <option value="advanced">Specify scoring parameters</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	147 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	148 <when value="defaults" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	149 <when value="advanced">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	150 <param name="match_bonus" label="Set match bonus" help="(--ma) In local mode N is added to the alignment score for each position where a read character aligns to a reference character and the characters match. Not used in end-to-end mode" type="integer" value="2" default="2" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	151 <param name="max_mismatch" label="Maximum mismatch penalty" help="(--mp) Sets the maximum mismatch penalty. A number less than or equal to MX and greater than or equal to MN is subtracted from the alignment score for each position where a read character aligns to a reference character, the characters do not match, and neither is an N. If --ignore-quals is specified, the number subtracted quals MX. Otherwise, the number subtracted is MN + floor( (MX-MN)(MIN(Q, 40.0)/40.0) ) where Q is the Phred quality value." type="integer" value="6" default="6" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	152 <param name="min_mismatch" label="Minimum mismatch penalty" help="(--mp) Sets the minimum mismatch penalty. A number less than or equal to MX and greater than or equal to MN is subtracted from the alignment score for each position where a read character aligns to a reference character, the characters do not match, and neither is an N. If --ignore-quals is specified, the number subtracted quals MX. Otherwise, the number subtracted is MN + floor( (MX-MN)(MIN(Q, 40.0)/40.0) ) where Q is the Phred quality value." type="integer" value="2" default="2" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	153 <param name="ambiguous_penalty" label="Ambiguous read penalty" help="(--np) Sets penalty for positions where the read, reference, or both, contain an ambiguous character such as N" type="integer" value="1" default="1" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	154 <param name="read_open_penalty" label="Read gap open penalty." type="integer" help="(--rdg) A read gap of length N gets a penalty of [open_penalty] + N * [extend_penalty]" value="5" default="5" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	155 <param name="read_extend_penalty" label="Read gap extend penalty." type="integer" help="(--rdg) A read gap of length N gets a penalty of [open_penalty] + N * [extend_penalty]" value="3" default="3" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	156 <param name="ref_open_penalty" label="Reference gap open penalty." help="(--rfg) A reference gap of length N gets a penalty of [open_penalty] + N * [extend_penalty]" type="integer" value="5" default="5" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	157 <param name="ref_extend_penalty" label="Reference gap extend penalty." help="(--rfg) A reference gap of length N gets a penalty of [open_penalty] + N * [extend_penalty]" type="integer" value="3" default="3" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	158 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	159 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	160 <conditional name="spliced_options">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	161 <param label="Spliced alignment parameters" name="spliced_options_selector" type="select">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	162 <option value="defaults">Use default values</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	163 <option value="advanced">Specify spliced alignment parameters</option>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	164 </param>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	165 <when value="defaults" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	166 <when value="advanced">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	167 <param name="canonical_penalty" label="Penalty for canonical splice sites" type="integer" value="0" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	168 <param name="noncanonical_penalty" label="Penalty for non-canonical splice sites" type="integer" value="3" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	169 <expand macro="function">
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	170 <label>Sets the penalty for long introns so that alignments with shorter introns are preferred to those with longer ones.</label>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	171 </expand>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	172 <param name="min_intron" label="Minimum intron length" type="integer" value="20" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	173 <param name="max_intron" label="Maximum intron length" type="integer" value="500000" />
1 8bb3efa0dae8 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 8000b1dd0d6b336707cf84867d11f690f6a26f5c devteam parents: 0 diff changeset	174 <param name="known_splice_gtf" label="GTF file with known splice sites" type="data" format="gtf" optional="True" />
0 e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	175 </when>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	176 </conditional>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	177 </inputs>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	178 <tests>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	179 <test>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	180 <param name="input_format_selector" value="fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	181 <param name="paired_selector" value="paired" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	182 <param name="reference_genome_source" value="history" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	183 <param name="history_item" value="phiX.fa" ftype="fasta" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	184 <param name="reads_f" ftype="fastqsanger" value="hisat_input_1_forward.fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	185 <param name="reads_r" ftype="fastqsanger" value="hisat_input_1_reverse.fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	186 <output name="output_alignments" ftype="bam" file="hisat_output_1.bam" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	187 </test>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	188 <test>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	189 <param name="input_format_selector" value="fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	190 <param name="paired_selector" value="paired" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	191 <param name="reference_genome_source" value="history" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	192 <param name="history_item" value="phiX.fa" ftype="fasta" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	193 <param name="input_options_selector" value="advanced" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	194 <param name="trim_three" value="15" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	195 <param name="trim_five" value="15" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	196 <param name="reads_f" ftype="fastqsanger" value="hisat_input_2_forward.fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	197 <param name="reads_r" ftype="fastqsanger" value="hisat_input_2_reverse.fastq" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	198 <output name="output_alignments" ftype="bam" file="hisat_output_2.bam" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	199 </test>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	200 </tests>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	201 <outputs>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	202 <data name="output_alignments" format="bam" />
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	203 </outputs>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	204 <help><![CDATA[
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	205 Introduction
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	206 ============
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	207
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	208 What is HISAT?
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	209 --------------
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	210
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	211 `HISAT <http://ccb.jhu.edu/software/hisat>`__ is a fast and sensitive
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	212 spliced alignment program. As part of HISAT, we have developed a new
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	213 indexing scheme based on the Burrows-Wheeler transform
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	214 (`BWT <http://en.wikipedia.org/wiki/Burrows-Wheeler_transform>`__) and
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	215 the `FM index <http://en.wikipedia.org/wiki/FM-index>`__, called
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	216 hierarchical indexing, that employs two types of indexes: (1) one global
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	217 FM index representing the whole genome, and (2) many separate local FM
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	218 indexes for small regions collectively covering the genome. Our
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	219 hierarchical index for the human genome (about 3 billion bp) includes
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	220 ~48,000 local FM indexes, each representing a genomic region of
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	221 ~64,000bp. As the basis for non-gapped alignment, the FM index is
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	222 extremely fast with a low memory footprint, as demonstrated by
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	223 `Bowtie <http://bowtie-bio.sf.net>`__. In addition, HISAT provides
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	224 several alignment strategies specifically designed for mapping different
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	225 types of RNA-seq reads. All these together, HISAT enables extremely fast
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	226 and sensitive alignment of reads, in particular those spanning two exons
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	227 or more. As a result, HISAT is much faster >50 times than
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	228 `TopHat2 <http://ccb.jhu.edu/software/tophat>`__ with better alignment
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	229 quality. Although it uses a large number of indexes, the memory
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	230 requirement of HISAT is still modest, approximately 4.3 GB for human.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	231 HISAT uses the `Bowtie2 <http://bowtie-bio.sf.net/bowtie2>`__
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	232 implementation to handle most of the operations on the FM index. In
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	233 addition to spliced alignment, HISAT handles reads involving indels and
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	234 supports a paired-end alignment mode. Multiple processors can be used
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	235 simultaneously to achieve greater alignment speed. HISAT outputs
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	236 alignments in `SAM <http://samtools.sourceforge.net/SAM1.pdf>`__ format,
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	237 enabling interoperation with a large number of other tools (e.g.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	238 `SAMtools <http://samtools.sourceforge.net>`__,
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	239 `GATK <http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit>`__)
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	240 that use SAM. HISAT is distributed under the `GPLv3
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	241 license <http://www.gnu.org/licenses/gpl-3.0.html>`__, and it runs on
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	242 the command line under Linux, Mac OS X and Windows.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	243
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	244 Running HISAT
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	245 =============
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	246
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	247 Reporting
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	248 ---------
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	249
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	250 The reporting mode governs how many alignments HISAT looks for, and how
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	251 to report them.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	252
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	253 In general, when we say that a read has an alignment, we mean that it
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	254 has a `valid
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	255 alignment <#valid-alignments-meet-or-exceed-the-minimum-score-threshold>`__.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	256 When we say that a read has multiple alignments, we mean that it has
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	257 multiple alignments that are valid and distinct from one another.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	258
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	259 Distinct alignments map a read to different places
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	260 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	261
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	262 Two alignments for the same individual read are "distinct" if they map
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	263 the same read to different places. Specifically, we say that two
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	264 alignments are distinct if there are no alignment positions where a
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	265 particular read offset is aligned opposite a particular reference offset
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	266 in both alignments with the same orientation. E.g. if the first
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	267 alignment is in the forward orientation and aligns the read character at
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	268 read offset 10 to the reference character at chromosome 3, offset
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	269 3,445,245, and the second alignment is also in the forward orientation
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	270 and also aligns the read character at read offset 10 to the reference
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	271 character at chromosome 3, offset 3,445,245, they are not distinct
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	272 alignments.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	273
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	274 Two alignments for the same pair are distinct if either the mate 1s in
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	275 the two paired-end alignments are distinct or the mate 2s in the two
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	276 alignments are distinct or both.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	277
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	278 Default mode: search for one or more alignments, report each
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	279 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	280
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	281 HISAT searches for up to N distinct, primary alignments for each read,
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	282 where N equals the integer specified with the ``-k`` parameter. Primary
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	283 alignments mean alignments whose alignment score is equal or higher than
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	284 any other alignments. It is possible that multiple distinct alignments
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	285 whave the same score. That is, if ``-k 2`` is specified, HISAT will
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	286 search for at most 2 distinct alignments. The alignment score for a
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	287 paired-end alignment equals the sum of the alignment scores of the
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	288 individual mates. Each reported read or pair alignment beyond the first
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	289 has the SAM 'secondary' bit (which equals 256) set in its FLAGS field.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	290 See the `SAM specification <http://samtools.sourceforge.net/SAM1.pdf>`__
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	291 for details.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	292
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	293 HISAT does not "find" alignments in any specific order, so for reads
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	294 that have more than N distinct, valid alignments, HISAT does not
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	295 gaurantee that the N alignments reported are the best possible in terms
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	296 of alignment score. Still, this mode can be effective and fast in
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	297 situations where the user cares more about whether a read aligns (or
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	298 aligns a certain number of times) than where exactly it originated.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	299
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	300 Alignment summmary
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	301 ------------------
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	302
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	303 When HISAT finishes running, it prints messages summarizing what
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	304 happened. These messages are printed to the "standard error" ("stderr")
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	305 filehandle. For datasets consisting of unpaired reads, the summary might
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	306 look like this:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	307
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	308 ::
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	309
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	310 20000 reads; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	311 20000 (100.00%) were unpaired; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	312 1247 (6.24%) aligned 0 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	313 18739 (93.69%) aligned exactly 1 time
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	314 14 (0.07%) aligned >1 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	315 93.77% overall alignment rate
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	316
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	317 For datasets consisting of pairs, the summary might look like this:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	318
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	319 ::
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	320
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	321 10000 reads; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	322 10000 (100.00%) were paired; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	323 650 (6.50%) aligned concordantly 0 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	324 8823 (88.23%) aligned concordantly exactly 1 time
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	325 527 (5.27%) aligned concordantly >1 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	326 ----
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	327 650 pairs aligned concordantly 0 times; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	328 34 (5.23%) aligned discordantly 1 time
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	329 ----
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	330 616 pairs aligned 0 times concordantly or discordantly; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	331 1232 mates make up the pairs; of these:
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	332 660 (53.57%) aligned 0 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	333 571 (46.35%) aligned exactly 1 time
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	334 1 (0.08%) aligned >1 times
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	335 96.70% overall alignment rate
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	336
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	337 The indentation indicates how subtotals relate to totals.
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	338 ]]></help>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	339 <citations>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	340 <citation type="doi">10.1038/nmeth.3317</citation>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	341 </citations>
e3fe061597ac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/hisat commit 5a7365750648c26206f05ac7956936c243c2b980 devteam parents: diff changeset	342 </tool>

Mercurial > repos > devteam > hisat

annotate hisat.xml @ 3:0505bdb7d6b1 draft default tip