5
|
1 <tool id="picard_CleanSam" name="CleanSam" version="1.126.0">
|
|
2
|
|
3 <description>perform SAM/BAM grooming</description>
|
|
4
|
|
5 <requirements>
|
|
6 <requirement type="package" version="1.126.0">picard</requirement>
|
|
7 </requirements>
|
|
8
|
|
9 <macros>
|
|
10 <import>picard_macros.xml</import>
|
|
11 </macros>
|
|
12
|
|
13 <command>
|
|
14 @java_options@
|
|
15 java -jar \$JAVA_JAR_PATH/picard.jar
|
|
16 CleanSam
|
|
17 INPUT="${inputFile}"
|
|
18 OUTPUT="${outFile}"
|
|
19 QUIET=true
|
|
20 VERBOSITY=ERROR
|
|
21 VALIDATION_STRINGENCY=${validation_stringency}
|
|
22 </command>
|
|
23
|
|
24 <inputs>
|
|
25 <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/>
|
|
26
|
|
27 <expand macro="VS" />
|
|
28
|
|
29 </inputs>
|
|
30
|
|
31 <outputs>
|
|
32 <data name="outFile" format="bam" label="${tool.name} on ${on_string}: cleaned BAM dataset">
|
|
33 </data>
|
|
34 </outputs>
|
|
35
|
|
36 <stdio>
|
|
37 <exit_code range="1:" level="fatal"/>
|
|
38 </stdio>
|
|
39
|
|
40 <tests>
|
|
41 <test>
|
|
42 <param name="inputFile" ftype="bam" value="picard_CleanSam.bam" />
|
|
43 <output name="outFile" file="picard_CleanSam_test1.bam" ftype="bam" />
|
|
44 </test>
|
|
45 </tests>
|
|
46
|
|
47 <help>
|
|
48
|
|
49 .. class:: infomark
|
|
50
|
|
51 **Purpose**
|
|
52
|
|
53 Read SAM/BAM and perform various fix-ups. Currently, the only fix-ups are:
|
|
54
|
|
55 1. to soft-clip an alignment that hangs off the end of its reference sequence.
|
|
56 2. to set MAPQ to 0 if a read is unmapped.
|
|
57
|
|
58 @dataset_collections@
|
|
59
|
|
60 @more_info@
|
|
61
|
|
62 </help>
|
|
63 </tool> |