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comparison picard_MergeBamAlignment.xml @ 33:3f254c5ced1d draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit 9ecbbb878d68a980ba35a90865e524c723ca3ed8
author | iuc |
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date | Sun, 03 Mar 2024 16:06:11 +0000 |
parents | f9242e01365a |
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32:f9242e01365a | 33:3f254c5ced1d |
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1 <tool name="MergeBamAlignment" id="picard_MergeBamAlignment" version="@TOOL_VERSION@.@WRAPPER_VERSION@"> | 1 <tool name="MergeBamAlignment" id="picard_MergeBamAlignment" version="@TOOL_VERSION@.@WRAPPER_VERSION@" profile="@PROFILE@"> |
2 <description>merge alignment data with additional info stored in an unmapped BAM dataset</description> | 2 <description>merge alignment data with additional info stored in an unmapped BAM dataset</description> |
3 <macros> | 3 <macros> |
4 <import>picard_macros.xml</import> | 4 <import>picard_macros.xml</import> |
5 <token name="@WRAPPER_VERSION@">2</token> | 5 <token name="@WRAPPER_VERSION@">0</token> |
6 </macros> | 6 </macros> |
7 <expand macro="requirements" /> | 7 <expand macro="requirements"/> |
8 <command detect_errors="exit_code"><![CDATA[ | 8 <command detect_errors="exit_code"><![CDATA[ |
9 @java_options@ | 9 @java_options@ |
10 #set $picard_dict = "localref.dict" | 10 #set $picard_dict = "localref.dict" |
11 #set $ref_fasta = "localref.fa" ## This is done because picards "likes" .fa extension | 11 #set $ref_fasta = "localref.fa" ## This is done because picards "likes" .fa extension |
12 | 12 |
13 ln -s "${reference_source.ref_file}" "${ref_fasta}" && | 13 ln -sf '${reference_source.ref_file}' '${ref_fasta}' && |
14 | 14 |
15 #if str( $reference_source.reference_source_selector ) == "history": | 15 #if str( $reference_source.reference_source_selector ) == "history": |
16 | 16 |
17 picard CreateSequenceDictionary REFERENCE="${ref_fasta}" OUTPUT="${picard_dict}" | 17 picard CreateSequenceDictionary |
18 QUIET=true | 18 -REFERENCE '${ref_fasta}' |
19 VERBOSITY=ERROR | 19 -OUTPUT '${picard_dict}' |
20 -QUIET true | |
21 -VERBOSITY ERROR | |
20 | 22 |
21 && | 23 && |
22 | 24 |
23 #else: | 25 #else: |
24 | 26 |
25 #set $ref_fasta = str( $reference_source.ref_file.fields.path ) | 27 #set $ref_fasta = str( $reference_source.ref_file.fields.path ) |
26 | 28 |
27 #end if | 29 #end if |
28 | 30 |
29 picard | 31 picard MergeBamAlignment |
30 MergeBamAlignment | 32 --UNMAPPED_BAM '${unmapped_bam}' |
31 UNMAPPED_BAM="${unmapped_bam}" | 33 |
32 | 34 --PAIRED_RUN true ##This argument is ignored and will be removed. Required. Possible values: {true, false} |
33 PAIRED_RUN=true ##This argument is ignored and will be removed. Required. Possible values: {true, false} | |
34 | 35 |
35 #if str( $aligned_or_read1_and_read2.aligned_or_read1_and_read2_selector ) == "paired_one_file": | 36 #if str( $aligned_or_read1_and_read2.aligned_or_read1_and_read2_selector ) == "paired_one_file": |
36 #for $dataset in $aligned_or_read1_and_read2.aligned_bams: | 37 #for $dataset in $aligned_or_read1_and_read2.aligned_bams: |
37 ALIGNED_BAM="${dataset.aligned_bam}" | 38 --ALIGNED_BAM '${dataset.aligned_bam}' |
38 #end for | 39 #end for |
39 #elif str( $aligned_or_read1_and_read2.aligned_or_read1_and_read2_selector ) == "paired_two_files": | 40 #elif str( $aligned_or_read1_and_read2.aligned_or_read1_and_read2_selector ) == "paired_two_files": |
40 #for $dataset in $aligned_or_read1_and_read2.read1_aligned_bams: | 41 #for $dataset in $aligned_or_read1_and_read2.read1_aligned_bams: |
41 READ1_ALIGNED_BAM="${dataset.read1_aligned_bam}" | 42 --READ1_ALIGNED_BAM '${dataset.read1_aligned_bam}' |
42 #end for | 43 #end for |
43 #for $dataset in $aligned_or_read1_and_read2.read2_aligned_bams: | 44 #for $dataset in $aligned_or_read1_and_read2.read2_aligned_bams: |
44 READ2_ALIGNED_BAM="${dataset.read2_aligned_bam}" | 45 --READ2_ALIGNED_BAM '${dataset.read2_aligned_bam}' |
45 #end for | 46 #end for |
46 #else | 47 #else |
47 #for $dataset in $aligned_or_read1_and_read2.read1_aligned_bams: | 48 #for $dataset in $aligned_or_read1_and_read2.read1_aligned_bams: |
48 READ1_ALIGNED_BAM="${dataset.read1_aligned_bam}" | 49 --READ1_ALIGNED_BAM '${dataset.read1_aligned_bam}' |
49 #end for | 50 #end for |
50 #end if | 51 #end if |
51 | 52 |
52 OUTPUT="${outFile}" | 53 --OUTPUT '${outFile}' |
53 REFERENCE_SEQUENCE="${ref_fasta}" | 54 --REFERENCE_SEQUENCE '${ref_fasta}' |
54 | 55 |
55 CLIP_ADAPTERS="${clip_adapters}" | 56 --CLIP_ADAPTERS '${clip_adapters}' |
56 IS_BISULFITE_SEQUENCE="${is_bisulfite_sequence}" | 57 --IS_BISULFITE_SEQUENCE '${is_bisulfite_sequence}' |
57 ALIGNED_READS_ONLY="${aligned_reads_only}" | 58 --ALIGNED_READS_ONLY '${aligned_reads_only}' |
58 MAX_INSERTIONS_OR_DELETIONS="${max_insertions_or_deletions}" | 59 --MAX_INSERTIONS_OR_DELETIONS '${max_insertions_or_deletions}' |
59 | 60 |
60 #for $attribute in $attributes_to_retain: | 61 #for $attribute in $attributes_to_retain: |
61 ATTRIBUTES_TO_RETAIN="${$attribute.attribute}" | 62 --ATTRIBUTES_TO_RETAIN '${$attribute.attribute}' |
62 #end for | 63 #end for |
63 | 64 |
64 #for $attribute in $attributes_to_remove: | 65 #for $attribute in $attributes_to_remove: |
65 ATTRIBUTES_TO_REMOVE="${$attribute.attribute}" | 66 --ATTRIBUTES_TO_REMOVE '${$attribute.attribute}' |
66 #end for | 67 #end for |
67 | 68 |
68 READ1_TRIM="${read1_trim}" | 69 --READ1_TRIM '${read1_trim}' |
69 READ2_TRIM="${read2_trim}" | 70 --READ2_TRIM '${read2_trim}' |
70 | 71 |
71 #if str( $orientations ) != "None": | 72 #if str( $orientations ) != "None": |
72 #for $orientation in str( $orientations ).split(','): ## See trello card https://trello.com/c/9nW02Zhd | 73 #for $orientation in str( $orientations ).split(','): ## See trello card https://trello.com/c/9nW02Zhd |
73 EXPECTED_ORIENTATIONS="${orientation}" | 74 --EXPECTED_ORIENTATIONS '${orientation}' |
74 #end for | 75 #end for |
75 #end if | 76 #end if |
76 | 77 |
77 ALIGNER_PROPER_PAIR_FLAGS="${aligner_proper_pair_flags}" | 78 --ALIGNER_PROPER_PAIR_FLAGS '${aligner_proper_pair_flags}' |
78 PRIMARY_ALIGNMENT_STRATEGY="${primary_alignment_strategy}" | 79 --PRIMARY_ALIGNMENT_STRATEGY '${primary_alignment_strategy}' |
79 CLIP_OVERLAPPING_READS="${clip_overlapping_reads}" | 80 --CLIP_OVERLAPPING_READS '${clip_overlapping_reads}' |
80 INCLUDE_SECONDARY_ALIGNMENTS="${include_secondary_alignments}" | 81 --INCLUDE_SECONDARY_ALIGNMENTS '${include_secondary_alignments}' |
81 ADD_MATE_CIGAR="${add_mate_cigar}" | 82 --ADD_MATE_CIGAR '${add_mate_cigar}' |
82 | 83 |
83 VALIDATION_STRINGENCY="${validation_stringency}" | 84 --VALIDATION_STRINGENCY '${validation_stringency}' |
84 | 85 |
85 SORT_ORDER=coordinate | 86 --SORT_ORDER coordinate |
86 QUIET=true | 87 --QUIET true |
87 VERBOSITY=ERROR | 88 --VERBOSITY ERROR |
88 | 89 |
89 ]]></command> | 90 ]]></command> |
90 | 91 <inputs> |
91 <inputs> | 92 <conditional name="reference_source"> |
92 | 93 <param name="reference_source_selector" type="select" label="Load reference genome from"> |
93 <conditional name="reference_source"> | 94 <option value="cached">Local cache</option> |
94 <param name="reference_source_selector" type="select" label="Load reference genome from"> | 95 <option value="history">History</option> |
95 <option value="cached">Local cache</option> | 96 </param> |
96 <option value="history">History</option> | 97 <when value="cached"> |
97 </param> | 98 <param name="ref_file" type="select" label="Use dictionary from the list" help="Select genome from the list"> |
98 <when value="cached"> | 99 <options from_data_table="picard_indexes"> |
99 <param name="ref_file" type="select" label="Use dictionary from the list" help="Select genome from the list"> | 100 <filter type="sort_by" column="2"/> |
100 <options from_data_table="picard_indexes"> | 101 <validator type="no_options" message="No indexes are available"/> |
101 <filter type="sort_by" column="2" /> | 102 </options> |
102 <validator type="no_options" message="No indexes are available" /> | 103 <validator type="no_options" message="A built-in dictionary is not available for the build associated with the selected input file"/> |
103 </options> | 104 </param> |
104 <validator type="no_options" message="A built-in dictionary is not available for the build associated with the selected input file"/> | 105 </when> |
106 <when value="history"> | |
107 <param name="ref_file" type="data" format="fasta" label="Use the following dataset to create dictionary" help="You can upload a FASTA sequence to the history from which Picard will automatically generate dictionary using CreateSequenceDictionary command"/> | |
108 </when> | |
109 </conditional> | |
110 <param format="sam,bam" name="unmapped_bam" type="data" label="Selected unaligned SAM or BAM with original reads" help="UNMAPPED_BAM; This dataset must be sorted in queryname order (use picard_SortSam to do this)"/> | |
111 <conditional name="aligned_or_read1_and_read2"> | |
112 <param name="aligned_or_read1_and_read2_selector" type="select" label="What type of aligned data do you have?"> | |
113 <option value="paired_one_file">Paired data in a single BAM file (ALIGNED_BAM)</option> | |
114 <option value="paired_two_files">Paired data in separate files (READ1_ALIGNED_BAM and READ2_ALIGNED_BAM)</option> | |
115 <option value="single_file">Singe end data (READ1_ALIGNED_BAM)</option> | |
116 </param> | |
117 <when value="paired_one_file"> | |
118 <repeat name="aligned_bams" title="Aligned SAM or BAM dataset" min="1" help="You can provide multiple datasets"> | |
119 <param name="aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data" help="ALIGNED_BAM"/> | |
120 </repeat> | |
121 </when> | |
122 <when value="paired_two_files"> | |
123 <repeat name="read1_aligned_bams" title="Aligned SAM or BAM dataset for Read 1" min="1" help="You can provide multiple datasets"> | |
124 <param name="read1_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data for Read1" help="READ1_ALIGNED_BAM"/> | |
125 </repeat> | |
126 <repeat name="read2_aligned_bams" title="Aligned SAM or BAM dataset for Read 2" min="1" help="You can provide multiple datasets"> | |
127 <param name="read2_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data for Read2" help="READ2_ALIGNED_BAM"/> | |
128 </repeat> | |
129 </when> | |
130 <when value="single_file"> | |
131 <repeat name="read1_aligned_bams" title="Aligned SAM or BAM dataset for Single Reads" min="1" help="You can provide multiple datasets"> | |
132 <param name="read1_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data" help="READ1_ALIGNED_BAM"/> | |
133 </repeat> | |
134 </when> | |
135 </conditional> | |
136 <param name="clip_adapters" type="boolean" checked="true" label="Whether to clip adapters where identified" help="CLIP_ADAPTERS; default=True"/> | |
137 <param name="is_bisulfite_sequence" type="boolean" label="Whether the data is from bisulfite sequencing (used when caculating the NM tag)" help="IS_BISULFITE_SEQUENCE; default=False"/> | |
138 <param name="aligned_reads_only" type="boolean" label="Whether to output only aligned reads" help="ALIGNED_READS_ONLY; default=False"/> | |
139 <param name="max_insertions_or_deletions" type="integer" value="1" label="The maximum number of insertions or deletions permitted for an alignment to be included" help="MAX_INSERTIONS_OR_DELETIONS; Alignments with more than this many insertions or deletions will be ignored. Set to -1 to allow any number of insertions or deletions. default=1"/> | |
140 <repeat name="attributes_to_retain" title="Retain the following alignment attribute" min="0" help="You can provide multiple attributes"> | |
141 <param name="attribute" type="text" label="Reserved alignment attributes (tags starting with X, Y, or Z) that should be brought over from the alignment data when merging" help="ATTRIBUTES_TO_RETAIN; example: XA"/> | |
142 </repeat> | |
143 <repeat name="attributes_to_remove" title="Remove the following alignment attribute" min="0" help="You can provide multiple attributes"> | |
144 <param name="attribute" type="text" label="Attributes from the alignment record that should be removed when merging." help="ATTRIBUTES_TO_REMOVE; This overrides ATTRIBUTES_TO_RETAIN if they share common tags"/> | |
145 </repeat> | |
146 <param name="read1_trim" type="integer" value="0" label="The number of bases trimmed from the beginning of read 1 prior to alignment" help="READ1_TRIM; default=0"/> | |
147 <param name="read2_trim" type="integer" value="0" label="The number of bases trimmed from the beginning of read 2 prior to alignment" help="READ2_TRIM; default=0"/> | |
148 <param name="orientations" type="select" multiple="True" display="checkboxes" label="The expected orientation of proper read pairs" help="EXPECTED_ORIENTATIONS; multiple orinetations can be selected"> | |
149 <option value="FR">Forward/Reverse (FR)</option> | |
150 <option value="RF">Reverse/Forward (RF)</option> | |
151 <option value="TANDEM">Tandem</option> | |
105 </param> | 152 </param> |
106 </when> | 153 <param name="aligner_proper_pair_flags" type="boolean" label="Use the aligner's idea of what a proper pair is rather than computing in this program" help="ALIGNER_PROPER_PAIR_FLAGS; default=False"/> |
107 <when value="history"> | 154 <param name="primary_alignment_strategy" type="select" label="Strategy for selecting primary alignment when the aligner has provided more than one alignment for a pair or fragments" help="PRIMARY_ALIGNMENT_STRATEGY; see help below for more info; default=BestMapq"> |
108 <param name="ref_file" type="data" format="fasta" label="Use the following dataset to create dictionary" help="You can upload a FASTA sequence to the history from which Picard will automatically generate dictionary using CreateSequenceDictionary command" /> | 155 <option value="BestMapq" selected="True">BestMapq</option> |
109 </when> | 156 <option value="EarliestFragment">EarliestFragment</option> |
110 </conditional> | 157 <option value="BestEndMapq">BestEndMapq</option> |
111 | 158 <option value="MostDistant">MostDistant</option> |
112 <param format="sam,bam" name="unmapped_bam" type="data" label="Selected unaligned SAM or BAM with original reads" help="UNMAPPED_BAM; This dataset must be sorted in queryname order (use picard_SortSam to do this)" /> | 159 </param> |
113 <conditional name="aligned_or_read1_and_read2"> | 160 <param name="clip_overlapping_reads" type="boolean" checked="True" label="For paired reads, soft clip the 3' end of each read if necessary so that it does not extend past the 5' end of its mate" help="CLIP_OVERLAPPING_READS; default=True"/> |
114 <param name="aligned_or_read1_and_read2_selector" type="select" label="What type of aligned data do you have?"> | 161 <param name="include_secondary_alignments" type="boolean" checked="True" label="If false, do not write secondary alignments to output" help="INCLUDE_SECONDARY_ALIGNMENTS; default=True"/> |
115 <option value="paired_one_file">Paired data in a single BAM file (ALIGNED_BAM)</option> | 162 <param name="add_mate_cigar" type="boolean" checked="True" label="Adds the mate CIGAR tag (MC) if true, does not if false" help="ADD_MATE_CIGAR; default=True"/> |
116 <option value="paired_two_files">Paired data in separate files (READ1_ALIGNED_BAM and READ2_ALIGNED_BAM)</option> | 163 <expand macro="VS"/> |
117 <option value="single_file">Singe end data (READ1_ALIGNED_BAM)</option> | 164 </inputs> |
118 </param> | 165 <outputs> |
119 <when value="paired_one_file"> | 166 <data name="outFile" format="bam" label="${tool.name} on ${on_string}: BAM"/> |
120 <repeat name="aligned_bams" title="Aligned SAM or BAM dataset" min="1" help="You can provide multiple datasets"> | 167 </outputs> |
121 <param name="aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data" help="ALIGNED_BAM"/> | 168 <tests> |
122 </repeat> | 169 <test> |
123 </when> | 170 <param name="reference_source_selector" value="history"/> |
124 <when value="paired_two_files"> | 171 <param name="ref_file" value="picard_MergeBamAlignment_ref.fa" ftype="fasta"/> |
125 <repeat name="read1_aligned_bams" title="Aligned SAM or BAM dataset for Read 1" min="1" help="You can provide multiple datasets"> | 172 <param name="unmapped_bam" value="picard_MergeBamAlignment_unaligned.bam" ftype="bam"/> |
126 <param name="read1_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data for Read1" help="READ1_ALIGNED_BAM"/> | 173 <param name="aligned_or_read1_and_read2_selector" value="paired_one_file"/> |
127 </repeat> | 174 <param name="aligned_bam" value="picard_MergeBamAlignment_aligned.bam" ftype="bam"/> |
128 <repeat name="read2_aligned_bams" title="Aligned SAM or BAM dataset for Read 2" min="1" help="You can provide multiple datasets"> | 175 <param name="clip_adapters" value="True"/> |
129 <param name="read2_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data for Read2" help="READ2_ALIGNED_BAM"/> | 176 <param name="is_bisulfite_sequence" value="False"/> |
130 </repeat> | 177 <param name="aligned_reads_only" value="False"/> |
131 </when> | 178 <param name="max_insertions_or_deletions" value="1"/> |
132 <when value="single_file"> | 179 <param name="read1_trim" value="0"/> |
133 <repeat name="read1_aligned_bams" title="Aligned SAM or BAM dataset for Single Reads" min="1" help="You can provide multiple datasets"> | 180 <param name="read2_trim" value="0"/> |
134 <param name="read1_aligned_bam" type="data" format="sam,bam" label="SAM or BAM dataset(s) with alignment data" help="READ1_ALIGNED_BAM"/> | 181 <param name="orientations" value="FR"/> |
135 </repeat> | 182 <param name="aligner_proper_pair_flags" value="False"/> |
136 </when> | 183 <param name="primary_alignment_strategy" value="BestMapq"/> |
137 </conditional> | 184 <param name="clip_overlapping_reads" value="True"/> |
138 | 185 <param name="include_secondary_alignments" value="True"/> |
139 <param name="clip_adapters" type="boolean" checked="true" label="Whether to clip adapters where identified" help="CLIP_ADAPTERS; default=True"/> | 186 <param name="add_mate_cigar" value="True"/> |
140 <param name="is_bisulfite_sequence" type="boolean" label="Whether the data is from bisulfite sequencing (used when caculating the NM tag)" help="IS_BISULFITE_SEQUENCE; default=False"/> | 187 <output name="outFile" file="picard_MergeBamAlignment_test1.bam" ftype="bam" lines_diff="4"/> |
141 <param name="aligned_reads_only" type="boolean" label="Whether to output only aligned reads" help="ALIGNED_READS_ONLY; default=False"/> | 188 </test> |
142 <param name="max_insertions_or_deletions" type="integer" value="1" label="The maximum number of insertions or deletions permitted for an alignment to be included" help="MAX_INSERTIONS_OR_DELETIONS; Alignments with more than this many insertions or deletions will be ignored. Set to -1 to allow any number of insertions or deletions. default=1"/> | 189 </tests> |
143 | 190 <help> |
144 <repeat name="attributes_to_retain" title="Retain the following alignment attribute" min="0" help="You can provide multiple attributes"> | |
145 <param name="attribute" type="text" label="Reserved alignment attributes (tags starting with X, Y, or Z) that should be brought over from the alignment data when merging" help="ATTRIBUTES_TO_RETAIN; example: XA"/> | |
146 </repeat> | |
147 | |
148 <repeat name="attributes_to_remove" title="Remove the following alignment attribute" min="0" help="You can provide multiple attributes"> | |
149 <param name="attribute" type="text" label="Attributes from the alignment record that should be removed when merging." help="ATTRIBUTES_TO_REMOVE; This overrides ATTRIBUTES_TO_RETAIN if they share common tags"/> | |
150 </repeat> | |
151 | |
152 <param name="read1_trim" type="integer" value="0" label="The number of bases trimmed from the beginning of read 1 prior to alignment" help="READ1_TRIM; default=0"/> | |
153 <param name="read2_trim" type="integer" value="0" label="The number of bases trimmed from the beginning of read 2 prior to alignment" help="READ2_TRIM; default=0"/> | |
154 | |
155 <param name="orientations" type="select" multiple="True" display="checkboxes" label="The expected orientation of proper read pairs" help="EXPECTED_ORIENTATIONS; multiple orinetations can be selected"> | |
156 <option value="FR">Forward/Reverse (FR)</option> | |
157 <option value="RF">Reverse/Forward (RF)</option> | |
158 <option value="TANDEM">Tandem</option> | |
159 </param> | |
160 | |
161 | |
162 <param name="aligner_proper_pair_flags" type="boolean" label="Use the aligner's idea of what a proper pair is rather than computing in this program" help="ALIGNER_PROPER_PAIR_FLAGS; default=False"/> | |
163 | |
164 <param name="primary_alignment_strategy" type="select" label="Strategy for selecting primary alignment when the aligner has provided more than one alignment for a pair or fragments" help="PRIMARY_ALIGNMENT_STRATEGY; see help below for more info; default=BestMapq"> | |
165 <option value="BestMapq" selected="True">BestMapq</option> | |
166 <option value="EarliestFragment">EarliestFragment</option> | |
167 <option value="BestEndMapq">BestEndMapq</option> | |
168 <option value="MostDistant">MostDistant</option> | |
169 </param> | |
170 | |
171 <param name="clip_overlapping_reads" type="boolean" checked="True" label="For paired reads, soft clip the 3' end of each read if necessary so that it does not extend past the 5' end of its mate" help="CLIP_OVERLAPPING_READS; default=True"/> | |
172 <param name="include_secondary_alignments" type="boolean" checked="True" label="If false, do not write secondary alignments to output" help="INCLUDE_SECONDARY_ALIGNMENTS; default=True"/> | |
173 <param name="add_mate_cigar" type="boolean" checked="True" label="Adds the mate CIGAR tag (MC) if true, does not if false" help="ADD_MATE_CIGAR; default=True"/> | |
174 <expand macro="VS" /> | |
175 </inputs> | |
176 <outputs> | |
177 <data name="outFile" format="bam" label="${tool.name} on ${on_string}: BAM"/> | |
178 </outputs> | |
179 <tests> | |
180 <test> | |
181 <param name="reference_source_selector" value="history" /> | |
182 <param name="ref_file" value="picard_MergeBamAlignment_ref.fa" ftype="fasta" /> | |
183 <param name="unmapped_bam" value="picard_MergeBamAlignment_unaligned.bam" ftype="bam"/> | |
184 <param name="aligned_or_read1_and_read2_selector" value="paired_one_file"/> | |
185 <param name="aligned_bam" value="picard_MergeBamAlignment_aligned.bam" ftype="bam"/> | |
186 <param name="clip_adapters" value="True"/> | |
187 <param name="is_bisulfite_sequence" value="False"/> | |
188 <param name="aligned_reads_only" value="False"/> | |
189 <param name="max_insertions_or_deletions" value="1"/> | |
190 <param name="read1_trim" value="0"/> | |
191 <param name="read2_trim" value="0"/> | |
192 <param name="orientations" value="FR"/> | |
193 <param name="aligner_proper_pair_flags" value="False"/> | |
194 <param name="primary_alignment_strategy" value="BestMapq"/> | |
195 <param name="clip_overlapping_reads" value="True"/> | |
196 <param name="include_secondary_alignments" value="True"/> | |
197 <param name="add_mate_cigar" value="True"/> | |
198 <output name="outFile" file="picard_MergeBamAlignment_test1.bam" ftype="bam" lines_diff="4"/> | |
199 </test> | |
200 </tests> | |
201 | |
202 | |
203 <help> | |
204 | 191 |
205 .. class:: infomark | 192 .. class:: infomark |
206 | 193 |
207 **Purpose** | 194 **Purpose** |
208 | 195 |
307 | 294 |
308 | 295 |
309 | 296 |
310 @more_info@ | 297 @more_info@ |
311 </help> | 298 </help> |
312 <expand macro="citations" /> | 299 <expand macro="citations"/> |
313 </tool> | 300 </tool> |