Mercurial > repos > devteam > picard
comparison picard_FilterSamReads.xml @ 13:7e6fd3d0f16e draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit bf94a1505c131fb3f67c867b6e1d886780efa42e
author | devteam |
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date | Tue, 06 Dec 2016 10:04:41 -0500 |
parents | 05087b27692a |
children | 465cbb0cf2eb |
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12:05087b27692a | 13:7e6fd3d0f16e |
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4 <import>picard_macros.xml</import> | 4 <import>picard_macros.xml</import> |
5 </macros> | 5 </macros> |
6 <expand macro="requirements" /> | 6 <expand macro="requirements" /> |
7 <command detect_errors="exit_code"><![CDATA[ | 7 <command detect_errors="exit_code"><![CDATA[ |
8 @java_options@ | 8 @java_options@ |
9 | 9 @symlink_element_identifier@ |
10 ##Sam Sorting is performed here because FilterSamReads requires input to be in query-sorted order | 10 ##Sam Sorting is performed here because FilterSamReads requires input to be in query-sorted order |
11 | 11 |
12 picard | 12 picard |
13 SortSam | 13 SortSam |
14 INPUT="${inputFile}" | 14 INPUT='$inputFile.element_identifier' |
15 OUTPUT=query_sorted_bam.bam | 15 OUTPUT=query_sorted_bam.bam |
16 SORT_ORDER=queryname | 16 SORT_ORDER=queryname |
17 VALIDATION_STRINGENCY=LENIENT | 17 VALIDATION_STRINGENCY=LENIENT |
18 QUIET=true | 18 QUIET=true |
19 VERBOSITY=ERROR | 19 VERBOSITY=ERROR |
20 | 20 |
21 && | 21 && |
22 | 22 |
23 picard | 23 picard |
24 FilterSamReads | 24 FilterSamReads |
25 INPUT=query_sorted_bam.bam | 25 INPUT=query_sorted_bam.bam |
26 FILTER="${filter_type.filter}" | 26 FILTER="${filter_type.filter}" |
27 | 27 |
28 #if ( str( $filter_type.filter ) == "includeReadList" or str( $filter_type.filter ) == "excludeReadList" ): | 28 #if ( str( $filter_type.filter ) == "includeReadList" or str( $filter_type.filter ) == "excludeReadList" ): |
29 READ_LIST_FILE="${filter_type.read_list_file}" | 29 READ_LIST_FILE="${filter_type.read_list_file}" |
30 #end if | 30 #end if |
31 | 31 |
32 OUTPUT="${outFile}" | 32 OUTPUT="${outFile}" |
33 SORT_ORDER=coordinate | 33 SORT_ORDER=coordinate |
34 VALIDATION_STRINGENCY="${validation_stringency}" | 34 VALIDATION_STRINGENCY="${validation_stringency}" |
35 QUIET=true | 35 QUIET=true |
36 VERBOSITY=ERROR | 36 VERBOSITY=ERROR |
37 | 37 |
38 ]]></command> | 38 ]]></command> |
39 <inputs> | 39 <inputs> |
40 <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> | 40 <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> |
41 <conditional name="filter_type"> | 41 <conditional name="filter_type"> |
42 <param name="filter" type="select" label="Select filtering type" help="FILTER; see Help for deatiled info"> | 42 <param name="filter" type="select" label="Select filtering type" help="FILTER; see Help for deatiled info"> |
52 </when> | 52 </when> |
53 <when value="excludeReadList"> | 53 <when value="excludeReadList"> |
54 <param name="read_list_file" type="data" format="tabular" label="Dataset containing read names that will be EXCLUDED in the output" help="READ_LIST_FILE"/> | 54 <param name="read_list_file" type="data" format="tabular" label="Dataset containing read names that will be EXCLUDED in the output" help="READ_LIST_FILE"/> |
55 </when> | 55 </when> |
56 </conditional> | 56 </conditional> |
57 | 57 |
58 <expand macro="VS" /> | 58 <expand macro="VS" /> |
59 | 59 |
60 </inputs> | 60 </inputs> |
61 | 61 |
62 <outputs> | 62 <outputs> |
63 <data format="bam" name="outFile" label="${tool.name} on ${on_string}: filtered BAM"/> | 63 <data format="bam" name="outFile" label="${tool.name} on ${on_string}: filtered BAM"/> |
64 </outputs> | 64 </outputs> |
65 | 65 |
66 <tests> | 66 <tests> |
67 <test> | 67 <test> |
68 <param name="inputFile" value="picard_FilterSamReads.bam" ftype="bam"/> | 68 <param name="inputFile" value="picard_FilterSamReads.bam" ftype="bam"/> |
69 <param name="filter" value="includeReadList"/> | 69 <param name="filter" value="includeReadList"/> |
70 <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/> | 70 <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/> |
77 <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/> | 77 <param name="read_list_file" value="picard_FilterSamReads_read_list_file.tab" ftype="tabular"/> |
78 <param name="validation_stringency" value="LENIENT"/> | 78 <param name="validation_stringency" value="LENIENT"/> |
79 <output name="outFile" file="picard_FilterSamReads_exclude_reads_test2.bam" ftype="bam" lines_diff="4"/> | 79 <output name="outFile" file="picard_FilterSamReads_exclude_reads_test2.bam" ftype="bam" lines_diff="4"/> |
80 </test> | 80 </test> |
81 </tests> | 81 </tests> |
82 | 82 |
83 | 83 |
84 <help> | 84 <help> |
85 | 85 |
86 **Purpose** | 86 **Purpose** |
87 | 87 |
88 Computes a number of metrics that are useful for evaluating coverage and performance of whole genome sequencing experiments. | 88 Computes a number of metrics that are useful for evaluating coverage and performance of whole genome sequencing experiments. |
91 | 91 |
92 .. class:: warningmark | 92 .. class:: warningmark |
93 | 93 |
94 **Warning on using this tool on BWA-MEM output** | 94 **Warning on using this tool on BWA-MEM output** |
95 | 95 |
96 This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts. | 96 This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts. |
97 | 97 |
98 @dataset_collections@ | 98 @dataset_collections@ |
99 | 99 |
100 @description@ | 100 @description@ |
101 | 101 |
102 FILTER=Filter Filter. Required. Possible values: | 102 FILTER=Filter Filter. Required. Possible values: |
103 includeAligned [OUTPUT SAM/BAM will contain aligned | 103 includeAligned [OUTPUT SAM/BAM will contain aligned |
104 reads only. (Note that *both* first and | 104 reads only. (Note that *both* first and |
105 second of paired reads must be aligned to be included | 105 second of paired reads must be aligned to be included |
106 in the OUTPUT SAM or BAM)], | 106 in the OUTPUT SAM or BAM)], |
107 | 107 |
108 excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only. | 108 excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only. |
109 (Note that *both* first and second of pair must be aligned to be | 109 (Note that *both* first and second of pair must be aligned to be |
110 excluded from the OUTPUT SAM or BAM)] | 110 excluded from the OUTPUT SAM or BAM)] |
111 | 111 |
112 includeReadList [OUTPUT SAM/BAM will contain reads | 112 includeReadList [OUTPUT SAM/BAM will contain reads |
113 that are supplied in the READ_LIST_FILE file] | 113 that are supplied in the READ_LIST_FILE file] |
114 | 114 |
115 excludeReadList [OUTPUT bam will contain | 115 excludeReadList [OUTPUT bam will contain |
116 reads that are *not* supplied in the READ_LIST_FILE file]} | 116 reads that are *not* supplied in the READ_LIST_FILE file]} |
117 | 117 |
118 READ_LIST_FILE=File | 118 READ_LIST_FILE=File |
119 RLF=File Read List File containing reads that will be included or excluded from the OUTPUT SAM or | 119 RLF=File Read List File containing reads that will be included or excluded from the OUTPUT SAM or |
120 BAM file. Default value: null. | 120 BAM file. Default value: null. |
121 | 121 |
122 @more_info@ | 122 @more_info@ |
123 | 123 |
124 </help> | 124 </help> |
125 </tool> | 125 </tool> |
126 | 126 |