Mercurial > repos > devteam > picard
comparison picard_SamToFastq.xml @ 32:f9242e01365a draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit 285fab1660daa944d6833ae1e059b30cb1e88309
author | iuc |
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date | Mon, 25 Sep 2023 08:32:17 +0000 |
parents | 585027e65f3b |
children | 3f254c5ced1d |
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31:585027e65f3b | 32:f9242e01365a |
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53 INCLUDE_NON_PRIMARY_ALIGNMENTS="${include_non_primary_alignments}" | 53 INCLUDE_NON_PRIMARY_ALIGNMENTS="${include_non_primary_alignments}" |
54 | 54 |
55 VALIDATION_STRINGENCY="${validation_stringency}" | 55 VALIDATION_STRINGENCY="${validation_stringency}" |
56 QUIET=true | 56 QUIET=true |
57 VERBOSITY=ERROR | 57 VERBOSITY=ERROR |
58 @TMPDIR_OPTION@ | |
59 | 58 |
60 ]]></command> | 59 ]]></command> |
61 <inputs> | 60 <inputs> |
62 | 61 |
63 <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> | 62 <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> |
102 <filter>output_type['single_or_paired'] == 'pe_sep'</filter> | 101 <filter>output_type['single_or_paired'] == 'pe_sep'</filter> |
103 </data> | 102 </data> |
104 </outputs> | 103 </outputs> |
105 | 104 |
106 <tests> | 105 <tests> |
107 <test> | 106 <test expect_num_outputs="5"> |
108 <param name="inputFile" value="picard_SamToFastq.bam" ftype="bam"/> | 107 <param name="inputFile" value="picard_SamToFastq.bam" ftype="bam"/> |
109 <param name="single_or_paired" value="pe_interleaved" /> | 108 <param name="single_or_paired" value="pe_interleaved" /> |
110 <param name="re_reverse" value="true"/> | 109 <param name="re_reverse" value="true"/> |
111 <param name="include_non_pf_reads" value="false"/> | 110 <param name="include_non_pf_reads" value="false"/> |
112 <param name="clipping_attribute" value="" /> | 111 <param name="clipping_attribute" value="" /> |
116 <param name="read2_trim" value="0" /> | 115 <param name="read2_trim" value="0" /> |
117 <param name="read2_max_bases_to_write" value="-1"/> | 116 <param name="read2_max_bases_to_write" value="-1"/> |
118 <param name="include_non_primary_alignments" value="false"/> | 117 <param name="include_non_primary_alignments" value="false"/> |
119 <output name="interleaved_fastq" file="picard_SamToFastq_test1.fq" ftype="fastqsanger"/> | 118 <output name="interleaved_fastq" file="picard_SamToFastq_test1.fq" ftype="fastqsanger"/> |
120 </test> | 119 </test> |
121 <test> | 120 <test expect_num_outputs="5"> |
122 <param name="inputFile" value="picard_SamToFastq.bam" ftype="bam"/> | 121 <param name="inputFile" value="picard_SamToFastq.bam" ftype="bam"/> |
123 <param name="single_or_paired" value="pe_sep" /> | 122 <param name="single_or_paired" value="pe_sep" /> |
124 <param name="re_reverse" value="true"/> | 123 <param name="re_reverse" value="true"/> |
125 <param name="include_non_pf_reads" value="false"/> | 124 <param name="include_non_pf_reads" value="false"/> |
126 <param name="clipping_attribute" value="" /> | 125 <param name="clipping_attribute" value="" /> |
132 <param name="include_non_primary_alignments" value="false"/> | 131 <param name="include_non_primary_alignments" value="false"/> |
133 <output name="fq1" file="picard_SamToFastq_1.fq" ftype="fastqsanger"/> | 132 <output name="fq1" file="picard_SamToFastq_1.fq" ftype="fastqsanger"/> |
134 <output name="fq2" file="picard_SamToFastq_2.fq" ftype="fastqsanger"/> | 133 <output name="fq2" file="picard_SamToFastq_2.fq" ftype="fastqsanger"/> |
135 <output name="fq_u" file="picard_SamToFastq_u.fq" ftype="fastqsanger"/> | 134 <output name="fq_u" file="picard_SamToFastq_u.fq" ftype="fastqsanger"/> |
136 </test> | 135 </test> |
137 <test> | 136 <test expect_num_outputs="5"> |
138 <param name="inputFile" value="picard_SamToFastq_se.bam" ftype="bam"/> | 137 <param name="inputFile" value="picard_SamToFastq_se.bam" ftype="bam"/> |
139 <param name="single_or_paired" value="se" /> | 138 <param name="single_or_paired" value="se" /> |
140 <param name="re_reverse" value="true"/> | 139 <param name="re_reverse" value="true"/> |
141 <param name="include_non_pf_reads" value="false"/> | 140 <param name="include_non_pf_reads" value="false"/> |
142 <param name="clipping_attribute" value="" /> | 141 <param name="clipping_attribute" value="" /> |
155 | 154 |
156 **Purpose** | 155 **Purpose** |
157 | 156 |
158 Extracts read sequences and qualities from the input SAM/BAM dataset and outputs them in Sanger fastq format. In the RE_REVERSE=True mode (default behavior), if the read is aligned and the alignment is to the reverse strand on the genome, the read's sequence from input SAM.BAM dataset will be reverse-complemented prior to writing it to fastq in order restore correctly the original read sequence as it was generated by the sequencer. | 157 Extracts read sequences and qualities from the input SAM/BAM dataset and outputs them in Sanger fastq format. In the RE_REVERSE=True mode (default behavior), if the read is aligned and the alignment is to the reverse strand on the genome, the read's sequence from input SAM.BAM dataset will be reverse-complemented prior to writing it to fastq in order restore correctly the original read sequence as it was generated by the sequencer. |
159 | 158 |
160 ----- | |
161 | |
162 .. class:: warningmark | 159 .. class:: warningmark |
160 | |
163 | 161 |
164 @dataset_collections@ | 162 @dataset_collections@ |
165 | 163 |
166 @description@ | 164 @description@ |
167 | 165 |