Mercurial > repos > devteam > picard
diff picard_MarkDuplicates.xml @ 12:05087b27692a draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 7491208ca0c917a053798a48c3e54c3e30e95d92
author | devteam |
---|---|
date | Sun, 27 Nov 2016 15:11:50 -0500 |
parents | 5eaa8a968300 |
children | 7e6fd3d0f16e |
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--- a/picard_MarkDuplicates.xml Wed Nov 11 12:23:17 2015 -0500 +++ b/picard_MarkDuplicates.xml Sun Nov 27 15:11:50 2016 -0500 @@ -4,33 +4,33 @@ <import>picard_macros.xml</import> </macros> <expand macro="requirements" /> - <command> + <command detect_errors="exit_code"><![CDATA[ @java_options@ - - java -jar \$JAVA_JAR_PATH/picard.jar + + picard MarkDuplicates - + INPUT="${inputFile}" OUTPUT="${outFile}" - + METRICS_FILE="${metrics_file}" #for $element in $comments: COMMENT="${element.comment}" #end for REMOVE_DUPLICATES="${remove_duplicates}" ASSUME_SORTED="${assume_sorted}" - + DUPLICATE_SCORING_STRATEGY="${duplicate_scoring_strategy}" - + #import pipes READ_NAME_REGEX=${ pipes.quote( str( $read_name_regex ) ) or "''" } OPTICAL_DUPLICATE_PIXEL_DISTANCE="${optical_duplicate_pixel_distance}" - + VALIDATION_STRINGENCY="${validation_stringency}" QUIET=true VERBOSITY=ERROR - - </command> + + ]]></command> <inputs> <param format="bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> <repeat name="comments" title="Comment" min="0" help="You can provide multiple comments"> @@ -54,14 +54,14 @@ <param name="optical_duplicate_pixel_distance" type="integer" value="100" min="0" max="500" label="The maximum offset between two duplicte clusters in order to consider them optical duplicates" help="OPTICAL_DUPLICATE_PIXEL_DISTANCE; default=100"/> <expand macro="VS" /> - - </inputs> - + + </inputs> + <outputs> <data format="txt" name="metrics_file" label="${tool.name} on ${on_string}: MarkDuplicate metrics"/> <data format="bam" name="outFile" label="${tool.name} on ${on_string}: MarkDuplicates BAM output"/> </outputs> - + <tests> <test> <param name="inputFile" value="picard_MarkDuplicates.bam" ftype="bam"/> @@ -72,14 +72,11 @@ <param name="optical_duplicate_pixel_distance" value="100"/> <param name="duplicate_scoring_strategy" value="SUM_OF_BASE_QUALITIES"/> <param name="validation_stringency" value="LENIENT"/> - <output name="outFile" file="picard_MarkDuplicates_test1.bam" ftype="bam" lines_diff="2"/> + <output name="outFile" file="picard_MarkDuplicates_test1.bam" ftype="bam" lines_diff="4"/> </test> </tests> - - <stdio> - <exit_code range="1:" level="fatal"/> - </stdio> - + + <help> **Purpose** @@ -90,34 +87,34 @@ @description@ - COMMENT=String - CO=String Comment(s) to include in the output file's header. This option may be specified 0 or - more times. + COMMENT=String + CO=String Comment(s) to include in the output file's header. This option may be specified 0 or + more times. + + REMOVE_DUPLICATES=Boolean If true do not write duplicates to the output file instead of writing them with + appropriate flags set. Default value: false. - REMOVE_DUPLICATES=Boolean If true do not write duplicates to the output file instead of writing them with - appropriate flags set. Default value: false. - - READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read - names are parsed to extract three variables: tile/region, x coordinate and y coordinate. - These values are used to estimate the rate of optical duplication in order to give a more - accurate estimated library size. Set this option to null to disable optical duplicate - detection. The regular expression should contain three capture groups for the three - variables, in order. It must match the entire read name. Note that if the default regex - is specified, a regex match is not actually done, but instead the read name is split on - colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be - tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements - are assumed to be tile, x and y values. Default value: + READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read + names are parsed to extract three variables: tile/region, x coordinate and y coordinate. + These values are used to estimate the rate of optical duplication in order to give a more + accurate estimated library size. Set this option to null to disable optical duplicate + detection. The regular expression should contain three capture groups for the three + variables, in order. It must match the entire read name. Note that if the default regex + is specified, a regex match is not actually done, but instead the read name is split on + colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be + tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements + are assumed to be tile, x and y values. Default value: [a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*. - + DUPLICATE_SCORING_STRATEGY=ScoringStrategy - DS=ScoringStrategy The scoring strategy for choosing the non-duplicate among candidates. Default value: - SUM_OF_BASE_QUALITIES. Possible values: {SUM_OF_BASE_QUALITIES, TOTAL_MAPPED_REFERENCE_LENGTH} - + DS=ScoringStrategy The scoring strategy for choosing the non-duplicate among candidates. Default value: + SUM_OF_BASE_QUALITIES. Possible values: {SUM_OF_BASE_QUALITIES, TOTAL_MAPPED_REFERENCE_LENGTH} + OPTICAL_DUPLICATE_PIXEL_DISTANCE=Integer - The maximum offset between two duplicte clusters in order to consider them optical - duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels) - unless using later versions of the Illumina pipeline that multiply pixel values by 10, in - which case 50-100 is more normal. Default value: 100. + The maximum offset between two duplicte clusters in order to consider them optical + duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels) + unless using later versions of the Illumina pipeline that multiply pixel values by 10, in + which case 50-100 is more normal. Default value: 100. @more_info@