Mercurial > repos > devteam > picard
diff picard_ReorderSam.xml @ 5:3d4f1fa26f0e draft
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author | devteam |
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date | Tue, 16 Dec 2014 19:03:21 -0500 |
parents | 9227b8c3093b |
children | 3a3234d7a2e8 |
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--- a/picard_ReorderSam.xml Fri Feb 21 12:07:49 2014 -0500 +++ b/picard_ReorderSam.xml Tue Dec 16 19:03:21 2014 -0500 @@ -1,155 +1,120 @@ -<tool name="Reorder SAM/BAM" id="picard_ReorderSam" version="1.56.0"> - <requirements><requirement type="package" version="1.56.0">picard</requirement></requirements> - <command interpreter="python"> - picard_wrapper.py - --input="${inputFile}" - #if $source.indexSource == "built-in" - --ref="${source.ref.fields.path}" - #else - --ref-file="${refFile}" - --species-name="${source.speciesName}" - --build-name="${source.buildName}" - --trunc-names="${source.truncateSeqNames}" - #end if - --allow-inc-dict-concord="${allowIncDictConcord}" - --allow-contig-len-discord="${allowContigLenDiscord}" - --output-format="${outputFormat}" - --output="${outFile}" - --tmpdir "${__new_file_path__}" - -j "\$JAVA_JAR_PATH/ReorderSam.jar" +<tool name="ReorderSam" id="picard_ReorderSam" version="1.126.0"> + <description>reorder reads to match ordering in reference sequences</description> + <requirements><requirement type="package" version="1.126.0">picard</requirement></requirements> + + <macros> + <import>picard_macros.xml</import> + </macros> + + <command> + @java_options@ + #set $picard_dict = "localref.dict" + #set $ref_fasta = "localref.fa" ## This is done because picards "likes" .fa extension + + ln -s "${reference_source.ref_file}" "${ref_fasta}" && + + #if str( $reference_source.reference_source_selector ) == "history": + + java -jar \$JAVA_JAR_PATH/picard.jar CreateSequenceDictionary REFERENCE="${ref_fasta}" OUTPUT="${picard_dict}" + QUIET=true + VERBOSITY=ERROR + + && + + #else: + + #set $ref_fasta = str( $reference_source.ref_file.fields.path ) + + #end if + + java -jar \$JAVA_JAR_PATH/picard.jar + ReorderSam + INPUT="${inputFile}" + OUTPUT="${outFile}" + REFERENCE="${ref_fasta}" + ALLOW_INCOMPLETE_DICT_CONCORDANCE="${allow_incomplete_dict_concordance}" + ALLOW_CONTIG_LENGTH_DISCORDANCE="${allow_contig_length_discordance}" + + VALIDATION_STRINGENCY="${validation_stringency}" + QUIET=true + VERBOSITY=ERROR + </command> + <inputs> - <param format="bam,sam" name="inputFile" type="data" label="SAM/BAM dataset to be reordered" - help="If empty, upload or import a SAM/BAM dataset." /> - <conditional name="source"> - <param name="indexSource" type="select" label="Select Reference Genome" help="This tool will re-order SAM/BAM in the same order as reference selected below."> - <option value="built-in">Locally cached</option> + + <conditional name="reference_source"> + <param name="reference_source_selector" type="select" label="Load reference genome from"> + <option value="cached">Local cache</option> <option value="history">History</option> </param> - <when value="built-in"> - <param name="ref" type="select" label="Select a reference genome"> - <options from_data_table="picard_indexes" /> + <when value="cached"> + <param name="ref_file" type="select" label="Use dictionary from the list" help="Select genome from the list"> + <options from_data_table="picard_indexes"> + <filter type="sort_by" column="2" /> + <validator type="no_options" message="No indexes are available" /> + </options> + <validator type="no_options" message="A built-in dictionary is not available for the build associated with the selected input file"/> </param> </when> - <when value="history"> - <param name="refFile" type="data" format="fasta" metadata_name="dbkey" label="Using reference file" /> - <param name="speciesName" type="text" value="" label="Species name" /> - <param name="buildName" type="text" value="" label="Build name" /> - <param name="truncateSeqNames" type="boolean" checked="False" truevalue="true" falsevalue="false" label="Truncate sequence names after first whitespace" /> + <when value="history"> + <param name="ref_file" type="data" format="fasta" label="Use the following dataset to create dictionary" help="You can upload a FASTA sequence to the history from which Picard will automatically generate dictionary using CreateSequenceDictionary command" /> </when> </conditional> - <param name="allowIncDictConcord" type="boolean" checked="False" truevalue="true" falsevalue="false" label="Allow incomplete dict concordance?" help="Allows a partial overlap of the BAM contigs with the new reference sequence contigs." /> - <param name="allowContigLenDiscord" type="boolean" checked="False" truevalue="true" falsevalue="false" label="Allow contig length discordance?" help="This is dangerous--don't check it unless you know exactly what you're doing!" /> - <param name="outputFormat" type="boolean" checked="True" truevalue="bam" falsevalue="sam" label="Output BAM instead of SAM" help="Uncheck for SAM output" /> + + <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> + <param name="allow_incomplete_dict_concordance" type="boolean" label="If true, then allows only a partial overlap of the BAM contigs with the new reference sequence contigs" help="ALLOW_INCOMPLETE_DICT_CONCORDANCE; By default, this tool requires a corresponding contig in the new reference for each read contig; default=False"/> + <param name="allow_contig_length_discordance" type="boolean" label="If true, then permits mapping from a read contig to a new reference contig with the same name but a different length" help="ALLOW_CONTIG_LENGTH_DISCORDANCE; HIGHLY DANGEROUS! Only use if you know what you are doing; default=False"/> + <expand macro="VS" /> + </inputs> <outputs> - <data name="outFile" format="bam" label="${tool.name} on ${on_string}: reordered ${outputFormat}"> - <change_format> - <when input="outputFormat" value="sam" format="sam" /> - </change_format> - </data> + <data name="outFile" format="bam" label="${tool.name} on ${on_string}: Reordered BAM"/> </outputs> <tests> <test> - <!-- Commands: - cp test-data/phiX.fasta . - samtools faidx phiX.fasta - java -jar CreateSequenceDictionary.jar R=phiX.fasta O=phiX.dict URI=phiX.fasta TRUNCATE_NAMES_AT_WHITESPACE=false SPECIES=phiX174 - java -jar ReorderSam.jar VALIDATION_STRINGENCY=LENIENT I=test-data/picard_RS_input1.bam O=picard_RS_output1.bam REFERENCE=phiX.fasta ALLOW_INCOMPLETE_DICT_CONCORDANCE=false ALLOW_CONTIG_LENGTH_DISCORDANCE=false - --> - <param name="inputFile" value="picard_RS_input1.bam" /> - <param name="indexSource" value="history" /> - <param name="refFile" value="phiX.fasta" /> - <param name="speciesName" value="phiX174" /> - <param name="buildName" value="" /> - <param name="truncateSeqNames" value="false" /> - <param name="allowIncDictConcord" value="false" /> - <param name="allowContigLenDiscord" value="false" /> - <param name="outputFormat" value="True" /> - <output name="outFile" file="picard_RS_output1.bam" ftype="bam" lines_diff="4" compare="contains" /> - </test> - <test> - <!-- Command: - java -jar ReorderSam.jar VALIDATION_STRINGENCY=LENIENT I=test-data/picard_RS_input2.sam O=picard_RS_output2.sam REFERENCE=/path/to/phiX/picard_index/phiX.fa ALLOW_INCOMPLETE_DICT_CONCORDANCE=false ALLOW_CONTIG_LENGTH_DISCORDANCE=false - /path/to/phiX/srma_index/phiX.fa is path to phiX.fa, phiX.fa.fai, and phiX.dict - --> - <param name="inputFile" value="picard_RS_input2.sam" /> - <param name="indexSource" value="built-in" /> - <param name="ref" value="phiX" /> - <param name="allowIncDictConcord" value="false" /> - <param name="allowContigLenDiscord" value="false" /> - <param name="outputFormat" value="False" /> - <output name="outFile" file="picard_RS_output2.sam" ftype="sam" lines_diff="4" sort="True" /> - </test> - <test> - <!-- Commands: - cp test-data/picard_RS_input4.fasta . - samtools faidx picard_RS_input4.fasta - java -jar CreateSequenceDictionary.jar R=picard_RS_input4.fasta O=picard_RS_input4.dict URI=picard_RS_input4.fasta TRUNCATE_NAMES_AT_WHITESPACE=true SPECIES=phiX174 GENOME_ASSEMBLY=phiX_buildBlah1.1 - java -jar ReorderSam.jar VALIDATION_STRINGENCY=LENIENT I=test-data/picard_RS_input3.bam O=picard_RS_output3.sam REFERENCE=picard_RS_input4.fasta ALLOW_INCOMPLETE_DICT_CONCORDANCE=true ALLOW_CONTIG_LENGTH_DISCORDANCE=false - picard_RS_input3.bam can be made from picard_RS_input3.sam - --> - <param name="inputFile" value="picard_RS_input3.bam" /> - <param name="indexSource" value="history" /> - <param name="refFile" value="picard_RS_input4.fasta" /> - <param name="speciesName" value="phiX174" /> - <param name="buildName" value="phiX_buildBlah1.1" /> - <param name="truncateSeqNames" value="true" /> - <param name="allowIncDictConcord" value="true" /> - <param name="allowContigLenDiscord" value="false" /> - <param name="outputFormat" value="False" /> - <output name="outFile" file="picard_RS_output3.sam" ftype="sam" lines_diff="12" sort="True" /> + <param name="reference_source_selector" value="history" /> + <param name="ref_file" value="picard_ReorderSam_ref.fa" ftype="fasta" /> + <param name="inputFile" value="picard_ReorderSam.bam" ftype="bam"/> + <param name="allow_incomplete_dict_concordance" value="false"/> + <param name="allow_contig_length_discordance" value="false"/> + <output name="outFile" file="picard_ReorderSam_test1.bam" ftype="bam" lines_diff="2"/> </test> </tests> + + <stdio> + <exit_code range="1:" level="fatal"/> + </stdio> + <help> .. class:: infomark **Purpose** -Reorder SAM/BAM to match contig ordering in a particular reference file. Note that this is -not the same as sorting as done by the SortSam tool, which sorts by either coordinate -values or query name. The ordering in ReorderSam is based on exact name matching of -contigs/chromosomes. Reads that are mapped to a contig that is not in the new reference file are -not included in the output. - -**Picard documentation** - -This is a Galaxy wrapper for ReorderSam, a part of the external package Picard-tools_. - - .. _Picard-tools: http://www.google.com/search?q=picard+samtools - ------- - -.. class:: infomark +ReorderSam reorders reads in a SAM/BAM file to match the contig ordering in a provided reference file, as determined by exact name matching of contigs. Reads mapped to contigs absent in the new reference are dropped. -**Inputs, outputs, and parameters** - -For the file that needs to be reordered, either a sam file or a bam file must be supplied. -If a bam file is used, it must be coordinate-sorted. A reference file is also required, -so either a fasta file should be supplied or a built-in reference can be selected. +@dataset_collections@ -The output contains the same reads as the input file but the reads have been rearranged so -they appear in the same order as the provided reference file. The tool will output either -bam (the default) or sam, according to user selection. Bam is recommended since it is smaller. - -The only extra parameters that can be set are flags for allowing incomplete dict concordance -and allowing contig length discordance. If incomplete dict concordance is allowed, only a -partial overlap of the bam contigs with the new reference sequence contigs is required. By -default it is off, requiring a corresponding contig in the new reference for each read contig. -If contig length discordance is allowed, contig names that are the same between a read and the -new reference contig are allowed even if they have different lengths. This is usually not a -good idea, unless you know exactly what you're doing. It's off by default. +---- .. class:: warningmark -**Warning on SAM/BAM quality** +Not to be confused with **SortSam**. + +@description@ -Many SAM/BAM files produced externally and uploaded to Galaxy do not fully conform to SAM/BAM specifications. Galaxy deals with this by using the **LENIENT** -flag when it runs Picard, which allows reads to be discarded if they're empty or don't map. This appears -to be the only way to deal with SAM/BAM that cannot be parsed. + ALLOW_INCOMPLETE_DICT_CONCORDANCE=Boolean + S=Boolean If true, then allows only a partial overlap of the BAM contigs with the new reference + sequence contigs. By default, this tool requires a corresponding contig in the new + reference for each read contig Default value: false. Possible values: {true, false} + + ALLOW_CONTIG_LENGTH_DISCORDANCE=Boolean + U=Boolean If true, then permits mapping from a read contig to a new reference contig with the same + name but a different length. Highly dangerous, only use if you know what you are doing. + Default value: false. Possible values: {true, false} - +@more_info@ </help> </tool> @@ -164,3 +129,4 @@ +