Mercurial > repos > devteam > picard
diff picard_CollectInsertSizeMetrics.xml @ 13:7e6fd3d0f16e draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit bf94a1505c131fb3f67c867b6e1d886780efa42e
author | devteam |
---|---|
date | Tue, 06 Dec 2016 10:04:41 -0500 |
parents | 05087b27692a |
children | 465cbb0cf2eb |
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--- a/picard_CollectInsertSizeMetrics.xml Sun Nov 27 15:11:50 2016 -0500 +++ b/picard_CollectInsertSizeMetrics.xml Tue Dec 06 10:04:41 2016 -0500 @@ -8,27 +8,28 @@ </expand> <command detect_errors="exit_code"><![CDATA[ @java_options@ + @symlink_element_identifier@ ##set up input files #set $reference_fasta_filename = "localref.fa" - + #if str( $reference_source.reference_source_selector ) == "history": ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && #else: #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) #end if - + picard CollectInsertSizeMetrics - INPUT="${inputFile}" + INPUT='$inputFile.element_identifier' OUTPUT="${outFile}" HISTOGRAM_FILE="${histFile}" DEVIATIONS="${deviations}" - + #if str( $hist_width ): HISTOGRAM_WIDTH="${hist_width}" #end if - + MINIMUM_PCT="${min_pct}" REFERENCE_SEQUENCE="${reference_fasta_filename}" ASSUME_SORTED="${assume_sorted}" @@ -37,7 +38,7 @@ VALIDATION_STRINGENCY="${validation_stringency}" QUIET=true VERBOSITY=ERROR - + ]]></command> <inputs> <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/> @@ -67,16 +68,16 @@ <option value="LIBRARY">Library</option> <option value="READ_GROUP">Read group</option> </param> - + <expand macro="VS" /> - + </inputs> - + <outputs> <data format="tabular" name="outFile"/> <data format="pdf" name="histFile"/> </outputs> - + <tests> <test> <param name="metric_accumulation_level" value="ALL_READS"/> @@ -90,8 +91,8 @@ <output name="outFile" file="picard_CollectInsertSizeMetrics_test1.tab" ftype="tabular" lines_diff="4"/> </test> </tests> - - + + <help> .. class:: infomark @@ -104,30 +105,30 @@ @description@ - - DEVIATIONS=Double Generate mean, sd and plots by trimming the data down to MEDIAN + - DEVIATIONS*MEDIAN_ABSOLUTE_DEVIATION. This is done because insert size data typically - includes enough anomalous values from chimeras and other artifacts to make the mean and - sd grossly misleading regarding the real distribution. Default value: 10.0. + + DEVIATIONS=Double Generate mean, sd and plots by trimming the data down to MEDIAN + + DEVIATIONS*MEDIAN_ABSOLUTE_DEVIATION. This is done because insert size data typically + includes enough anomalous values from chimeras and other artifacts to make the mean and + sd grossly misleading regarding the real distribution. Default value: 10.0. HISTOGRAM_WIDTH=Integer - W=Integer Explicitly sets the Histogram width, overriding automatic truncation of Histogram tail. - Also, when calculating mean and standard deviation, only bins <= Histogram_WIDTH will be - included. Default value: not set. + W=Integer Explicitly sets the Histogram width, overriding automatic truncation of Histogram tail. + Also, when calculating mean and standard deviation, only bins <= Histogram_WIDTH will be + included. Default value: not set. MINIMUM_PCT=Float - M=Float When generating the Histogram, discard any data categories (out of FR, TANDEM, RF) that - have fewer than this percentage of overall reads. (Range: 0 to 1). Default value: 0.05. + M=Float When generating the Histogram, discard any data categories (out of FR, TANDEM, RF) that + have fewer than this percentage of overall reads. (Range: 0 to 1). Default value: 0.05. METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel - LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE, - LIBRARY, READ_GROUP} This option may be specified 0 or more times. - + LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE, + LIBRARY, READ_GROUP} This option may be specified 0 or more times. + ASSUME_SORTED=Boolean - AS=Boolean If true (default), then the sort order in the header file will be ignored. Default - value: true. This option can be set to 'null' to clear the default value. Possible + AS=Boolean If true (default), then the sort order in the header file will be ignored. Default + value: true. This option can be set to 'null' to clear the default value. Possible values: {true, false} - + @more_info@ </help>