Mercurial > repos > devteam > picard
diff picard_CollectRnaSeqMetrics.xml @ 27:a1f0b3f4b781 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit 11b94d7cd69b919f16feb10bd4b830a963334ab8"
author | iuc |
---|---|
date | Wed, 19 Feb 2020 14:27:00 -0500 |
parents | f6ced08779c4 |
children | f9242e01365a |
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--- a/picard_CollectRnaSeqMetrics.xml Mon Feb 17 10:24:29 2020 -0500 +++ b/picard_CollectRnaSeqMetrics.xml Wed Feb 19 14:27:00 2020 -0500 @@ -2,7 +2,7 @@ <description> collect metrics about the alignment of RNA to various functional classes of loci in the genome</description> <macros> <import>picard_macros.xml</import> - <token name="@WRAPPER_VERSION@">1</token> + <token name="@WRAPPER_VERSION@">2</token> </macros> <expand macro="requirements"> <requirement type="package" version="3.4.1">r-base</requirement> @@ -22,18 +22,16 @@ ## The awk line below converts a file obtained from UCSC as specified in the tool help to refFlat format #if str($gene_reference_source.gene_reference_source_selector) == "gtf" - #if $gene_reference_source.refFlat.ext != 'gff3' - gtfToGenePred -genePredExt '${gene_reference_source.refFlat}' refFlat.tab.raw && + #if $gene_reference_source.refFlat.ext == 'gff3' + gff3ToGenePred '${gene_reference_source.refFlat}' refFlat.tab.raw && #else - gff3ToGenePred '${gene_reference_source.refFlat}' refFlat.tab.raw && + gtfToGenePred -genePredExt '${gene_reference_source.refFlat}' refFlat.tab.raw && #end if - grep -v '^#' refFlat.tab.raw | awk '{print $12"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10}' > refFlat.tab && #else - grep -v '^#' ${refFlat} | awk '{print $11"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10}' > refFlat.tab && + grep -v '^#' ${refFlat} | awk '{if ($3 == "+" || $3 == "-") print $11"\t"$1"\t"$2"\t"$3"\t"$4"\t"$5"\t"$6"\t"$7"\t"$8"\t"$9"\t"$10; else print}' > refFlat.tab && #end if - ## Start picard command @java_options@ @@ -145,6 +143,20 @@ <param name="rrna_fragment_percentage" value="0.8" /> <output name="outFile" file="picard_CollectRnaSeqMetrics_test1.tab" ftype="tabular" lines_diff="4"/> </test> + <test> + <param name="reference_source_selector" value="history"/> + <param name="ref_file" value="picard_CollectRnaSeqMetrics_ref.fa" ftype="fasta"/> + <param name="inputFile" value="picard_CollectRnaSeqMetrics.bam" ftype="bam"/> + <param name="assume_sorted" value="true" /> + + <param name="gene_reference_source_selector" value="refflat" /> + <param name="refFlat" value="picard_CollectRnaSeqMetrics.ucsc_output" /> + <param name="metric_accumulation_level" value="ALL_READS" /> + <param name="minimum_length" value="500" /> + <param name="strand_specificity" value="NONE" /> + <param name="rrna_fragment_percentage" value="0.8" /> + <output name="outFile" file="picard_CollectRnaSeqMetrics_test1.tab" ftype="tabular" lines_diff="4"/> + </test> <test> <param name="reference_source_selector" value="history"/>