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view picard_CollectAlignmentSummaryMetrics.xml @ 8:3a3234d7a2e8 draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
author | devteam |
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date | Thu, 16 Jul 2015 15:53:10 -0400 |
parents | 3d4f1fa26f0e |
children | 5eaa8a968300 |
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<tool name="Collect Alignment Summary Metrics" id="picard_CASM" version="@TOOL_VERSION@.0"> <description>writes a file containing summary alignment metrics</description> <macros> <import>picard_macros.xml</import> </macros> <expand macro="requirements" /> <command> @java_options@ ##set up input files #set $reference_fasta_filename = "localref.fa" #if str( $reference_source.reference_source_selector ) == "history": ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && #else: #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) #end if java -jar \$JAVA_JAR_PATH/picard.jar CollectAlignmentSummaryMetrics INPUT="${inputFile}" OUTPUT="${outFile}" MAX_INSERT_SIZE=${maxinsert} #for $sequence in $adapters: ADAPTER_SEQUENCE="${sequence.adapter}" #end for METRIC_ACCUMULATION_LEVEL="${metric_accumulation_level}" IS_BISULFITE_SEQUENCED="${bisulphite}" REFERENCE_SEQUENCE="${reference_fasta_filename}" ASSUME_SORTED="${assume_sorted}" VALIDATION_STRINGENCY="${validation_stringency}" QUIET=true VERBOSITY=ERROR </command> <inputs> <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/> <conditional name="reference_source"> <param name="reference_source_selector" type="select" label="Load reference genome from"> <option value="cached">Local cache</option> <option value="history">History</option> </param> <when value="cached"> <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE"> <options from_data_table="all_fasta"> </options> <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> </param> </when> <when value="history"> <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" /> </when> </conditional> <param name="metric_accumulation_level" type="select" label="The level(s) at which to accumulate metrics" multiple="true" help="METRIC_ACCUMULATION_LEVEL"> <option value="ALL_READS" selected="True">All reads</option> <option value="SAMPLE">Sample</option> <option value="LIBRARY">Library</option> <option value="READ_GROUP">Read group</option> </param> <param name="assume_sorted" type="boolean" label="Assume the input file is already sorted" checked="true" truevalue="true" falsevalue="false" help="ASSUME_SORTED"/> <param name="bisulphite" type="boolean" label="Input file contains Bisulphite sequenced reads" checked="false" falsevalue="false" truevalue="true" help="IS_BISULFITE_SEQUENCED"/> <repeat name="adapters" title="Adapter" min="0" help="You can provide multiple adaptor sequences"> <param name="adapter" type="text" size="50" label="Use this adaptor sequence" help="ADAPTER_SEQUENCE"/> </repeat> <param name="maxinsert" value="100000" type="integer" label="Larger paired end reads and inter-chromosomal pairs considered chimeric" size="20" help="MAX_INSERT_SIZE"/> <expand macro="VS" /> </inputs> <outputs> <data format="tabular" name="outFile" label="${tool.name} on ${on_string}: Summary stats"/> </outputs> <stdio> <exit_code range="1:" level="fatal"/> </stdio> <tests> <test> <param name="bisulphite" value="false" /> <param name="sorted" value="true" /> <param name="adaptors" value="" /> <param name="maxinsert" value="100000" /> <param name="reference_source_selector" value="history" /> <param name="ref_file" value="picard_CASM_ref.fa" /> <param name="inputFile" value="picard_CASM.bam" ftype="bam" /> <output name="outFile" file="picard_CASM_test1.tab" ftype="tabular" lines_diff="4"/> </test> </tests> <help> .. class:: infomark **Purpose** Reads a SAM or BAM file and writes a file containing summary alignment metrics. @dataset_collections@ @description@ MAX_INSERT_SIZE=Integer Paired end reads above this insert size will be considered chimeric along with inter-chromosomal pairs. Default value: 100000. ADAPTER_SEQUENCE=String List of adapter sequences to use when processing the alignment metrics This option may be specified 0 or more times. METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE, LIBRARY, READ_GROUP} This option may be specified 0 or more times. IS_BISULFITE_SEQUENCED=Boolean BS=Boolean Whether the SAM or BAM file consists of bisulfite sequenced reads. REFERENCE_SEQUENCE=File R=File Reference sequence fasta Default value: null. ASSUME_SORTED=Boolean AS=Boolean If true (default), then the sort order in the header file will be ignored. @more_info@ </help> </tool>