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| author | devteam |
|---|---|
| date | Thu, 25 Oct 2012 12:51:42 -0400 |
| parents | 1cd7f3b42609 |
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<style type="text/css"> tr.d0 td {background-color: oldlace; color: black;} tr.d1 td {background-color: aliceblue; color: black;} </style><?xml version="1.0" encoding="utf-8" ?> <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <meta name="generator" content="Galaxy picard_wrapper tool output - see http://getgalaxy.org/" /> <title></title> <link rel="stylesheet" href="/static/style/base.css" type="text/css" /> </head> <body> <div class="document"> Galaxy tool CollectAlignmentSummaryMetrics run at 11/11/2011 08:07:10</b><br/><b>The following output files were created (click the filename to view/download a copy):</b><hr/><table> <tr><td><a href="CollectAlignmentSummaryMetrics.log">CollectAlignmentSummaryMetrics.log</a></td></tr> <tr><td><a href="CollectAlignmentSummaryMetrics.metrics.txt">CollectAlignmentSummaryMetrics.metrics.txt</a></td></tr> </table><p/> <b>Picard on line resources</b><ul> <li><a href="http://picard.sourceforge.net/index.shtml">Click here for Picard Documentation</a></li> <li><a href="http://picard.sourceforge.net/picard-metric-definitions.shtml">Click here for Picard Metrics definitions</a></li></ul><hr/> <b>Picard output (transposed to make it easier to see)</b><hr/> <table cellpadding="3" > <tr class="d0"><td colspan="2">## net.sf.picard.metrics.StringHeader</td></tr><tr class="d1"><td colspan="2"># net.sf.picard.analysis.CollectAlignmentSummaryMetrics MAX_INSERT_SIZE=100000 ADAPTER_SEQUENCE=[AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG, IS_BISULFITE_SEQUENCED=false] INPUT=/data/tmp/tmpLLcl1w/database/files/000/dataset_2.dat OUTPUT=/data/home/rlazarus/galaxy/database/job_working_directory/3/dataset_3_files/CollectAlignmentSummaryMetrics.metrics.txt REFERENCE_SEQUENCE=/data/home/rlazarus/galaxy/database/job_working_directory/3/dataset_3_files/CollectAlignmentSummaryMetricsfq2hit.fasta_fake.fasta ASSUME_SORTED=true TMP_DIR=[/tmp] VALIDATION_STRINGENCY=LENIENT METRIC_ACCUMULATION_LEVEL=[ALL_READS] IS_BISULFITE_SEQUENCED=false STOP_AFTER=0 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false</td></tr><tr class="d0"><td colspan="2">## net.sf.picard.metrics.StringHeader</td></tr><tr class="d1"><td colspan="2"># Started on: Fri Nov 11 08:07:10 EST 2011</td></tr><tr class="d0"><td colspan="2">## METRICS CLASS net.sf.picard.analysis.AlignmentSummaryMetrics</td></tr><tr class="d0"><td>CATEGORY</td><td>FIRST_OF_PAIR </td></tr> <tr class="d1"><td>TOTAL_READS</td><td>4 </td></tr> <tr class="d0"><td>PF_READS</td><td>4 </td></tr> <tr class="d1"><td>PCT_PF_READS</td><td>1 </td></tr> <tr class="d0"><td>PF_NOISE_READS</td><td>0 </td></tr> <tr class="d1"><td>PF_READS_ALIGNED</td><td>4 </td></tr> <tr class="d0"><td>PCT_PF_READS_ALIGNED</td><td>1 </td></tr> <tr class="d1"><td>PF_ALIGNED_BASES</td><td>404 </td></tr> <tr class="d0"><td>PF_HQ_ALIGNED_READS</td><td>4 </td></tr> <tr class="d1"><td>PF_HQ_ALIGNED_BASES</td><td>404 </td></tr> <tr class="d0"><td>PF_HQ_ALIGNED_Q20_BASES</td><td>28 </td></tr> <tr class="d1"><td>PF_HQ_MEDIAN_MISMATCHES</td><td>78 </td></tr> <tr class="d0"><td>PF_MISMATCH_RATE</td><td>0.777228 </td></tr> <tr class="d1"><td>PF_HQ_ERROR_RATE</td><td>0.777228 </td></tr> <tr class="d0"><td>PF_INDEL_RATE</td><td>0 </td></tr> <tr class="d1"><td>MEAN_READ_LENGTH</td><td>101 </td></tr> <tr class="d0"><td>READS_ALIGNED_IN_PAIRS</td><td>3 </td></tr> <tr class="d1"><td>PCT_READS_ALIGNED_IN_PAIRS</td><td>0.75 </td></tr> <tr class="d0"><td>BAD_CYCLES</td><td>63 </td></tr> <tr class="d1"><td>STRAND_BALANCE</td><td>0.25 </td></tr> <tr class="d0"><td>PCT_CHIMERAS</td><td>0 </td></tr> <tr class="d1"><td>PCT_ADAPTER</td><td>0 </td></tr> <tr class="d0"><td>SAMPLE</td><td> </td></tr> <tr class="d1"><td>LIBRARY</td><td> </td></tr> <tr class="d0"><td>READ_GROUP </td><td> </td></tr> </table> <b>Picard Tool Run Log</b><hr/> <pre>INFO:root:## executing java -Xmx4g -jar /data/home/rlazarus/galaxy/tool-data/shared/jars/picard/CreateSequenceDictionary.jar REFERENCE=/tmp/CollectAlignmentSummaryMetricsfq2hit.fasta OUTPUT=/tmp/CollectAlignmentSummaryMetricsfq2hit.dict URI=dataset_1.dat TRUNCATE_NAMES_AT_WHITESPACE=None returned status 0 and nothing on stderr INFO:root:## executing java -Xmx4g -jar /data/home/rlazarus/galaxy/tool-data/shared/jars/picard/CollectAlignmentSummaryMetrics.jar VALIDATION_STRINGENCY=LENIENT ASSUME_SORTED=true ADAPTER_SEQUENCE= IS_BISULFITE_SEQUENCED=false MAX_INSERT_SIZE=100000 OUTPUT=/data/home/rlazarus/galaxy/database/job_working_directory/3/dataset_3_files/CollectAlignmentSummaryMetrics.metrics.txt R=/data/home/rlazarus/galaxy/database/job_working_directory/3/dataset_3_files/CollectAlignmentSummaryMetricsfq2hit.fasta_fake.fasta TMP_DIR=/tmp INPUT=/data/tmp/tmpLLcl1w/database/files/000/dataset_2.dat returned status 0 and nothing on stderr </pre><hr/>The freely available <a href="http://picard.sourceforge.net/command-line-overview.shtml">Picard software</a> generated all outputs reported here running as a <a href="http://getgalaxy.org">Galaxy</a> tool</div></body></html>