Mercurial > repos > devteam > picard
view picard_ReorderSam.xml @ 33:3f254c5ced1d draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/picard commit 9ecbbb878d68a980ba35a90865e524c723ca3ed8
author | iuc |
---|---|
date | Sun, 03 Mar 2024 16:06:11 +0000 |
parents | f9242e01365a |
children |
line wrap: on
line source
<tool name="ReorderSam" id="picard_ReorderSam" version="@TOOL_VERSION@.@WRAPPER_VERSION@" profile="@PROFILE@"> <description>reorder reads to match ordering in reference sequences</description> <macros> <import>picard_macros.xml</import> <token name="@WRAPPER_VERSION@">0</token> </macros> <xrefs> <xref type="bio.tools">picard_reordersam</xref> </xrefs> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ @java_options@ @symlink_element_identifier@ #set $picard_dict = "localref.dict" #set $ref_fasta = "localref.fa" ## This is done because picards "likes" .fa extension ln -sf '${reference_source.ref_file}' '${ref_fasta}' && #if str( $reference_source.reference_source_selector ) == "history": picard CreateSequenceDictionary --REFERENCE '${ref_fasta}' --OUTPUT '${picard_dict}' --QUIET true --VERBOSITY ERROR && #else: #set $ref_fasta = str( $reference_source.ref_file.fields.path ) #end if picard ReorderSam --INPUT '$escaped_element_identifier' --OUTPUT '${outFile}' --SEQUENCE_DICTIONARY '${ref_fasta}' --ALLOW_INCOMPLETE_DICT_CONCORDANCE '${allow_incomplete_dict_concordance}' --ALLOW_CONTIG_LENGTH_DISCORDANCE '${allow_contig_length_discordance}' --VALIDATION_STRINGENCY '${validation_stringency}' --QUIET true --VERBOSITY ERROR ]]></command> <inputs> <conditional name="reference_source"> <param name="reference_source_selector" type="select" label="Load reference genome from"> <option value="cached">Local cache</option> <option value="history">History</option> </param> <when value="cached"> <param name="ref_file" type="select" label="Use dictionary from the list" help="Select genome from the list"> <options from_data_table="picard_indexes"> <filter type="sort_by" column="2"/> <validator type="no_options" message="No indexes are available"/> </options> <validator type="no_options" message="A built-in dictionary is not available for the build associated with the selected input file"/> </param> </when> <when value="history"> <param name="ref_file" type="data" format="fasta,bam,sam" label="Use the following dataset to create dictionary" help="You can upload sequences to the history from which Picard will automatically generate dictionary using CreateSequenceDictionary command"/> </when> </conditional> <param name="inputFile" type="data" format="sam,bam" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset"/> <param name="allow_incomplete_dict_concordance" type="boolean" label="If true, then allows only a partial overlap of the BAM contigs with the new reference sequence contigs" help="ALLOW_INCOMPLETE_DICT_CONCORDANCE; By default, this tool requires a corresponding contig in the new reference for each read contig; default=False"/> <param name="allow_contig_length_discordance" type="boolean" label="If true, then permits mapping from a read contig to a new reference contig with the same name but a different length" help="ALLOW_CONTIG_LENGTH_DISCORDANCE; HIGHLY DANGEROUS! Only use if you know what you are doing; default=False"/> <expand macro="VS"/> </inputs> <outputs> <data name="outFile" format="bam" label="${tool.name} on ${on_string}: Reordered BAM"/> </outputs> <tests> <test> <param name="reference_source_selector" value="history"/> <param name="ref_file" value="picard_ReorderSam_ref.fa" ftype="fasta"/> <param name="inputFile" value="picard_ReorderSam.bam" ftype="bam"/> <param name="allow_incomplete_dict_concordance" value="false"/> <param name="allow_contig_length_discordance" value="false"/> <output name="outFile" file="picard_ReorderSam_test1.bam" ftype="bam" lines_diff="4"/> </test> </tests> <help> .. class:: infomark **Purpose** ReorderSam reorders reads in a SAM/BAM file to match the contig ordering in a provided reference file, as determined by exact name matching of contigs. Reads mapped to contigs absent in the new reference are dropped. @dataset_collections@ ---- .. class:: warningmark Not to be confused with **SortSam**. @description@ ALLOW_INCOMPLETE_DICT_CONCORDANCE=Boolean S=Boolean If true, then allows only a partial overlap of the BAM contigs with the new reference sequence contigs. By default, this tool requires a corresponding contig in the new reference for each read contig Default value: false. Possible values: {true, false} ALLOW_CONTIG_LENGTH_DISCORDANCE=Boolean U=Boolean If true, then permits mapping from a read contig to a new reference contig with the same name but a different length. Highly dangerous, only use if you know what you are doing. Default value: false. Possible values: {true, false} @more_info@ </help> <expand macro="citations"/> </tool>