# HG changeset patch
# User devteam
# Date 1481036681 18000
# Node ID 7e6fd3d0f16ec084d615d2f6bbf04febe64e2036
# Parent 05087b27692a01f1edb80c8d74ee00b0961d0ad0
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit bf94a1505c131fb3f67c867b6e1d886780efa42e
diff -r 05087b27692a -r 7e6fd3d0f16e picard_AddCommentsToBam.xml
--- a/picard_AddCommentsToBam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_AddCommentsToBam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,9 +6,10 @@
-
+
-
+
-
+
-
-
+
+
@@ -66,42 +67,42 @@
@description@
INPUT=File
- I=File Input file (bam or sam). Required.
+ I=File Input file (bam or sam). Required.
OUTPUT=File
- O=File Output file (bam or sam). Required.
+ O=File Output file (bam or sam). Required.
SORT_ORDER=SortOrder
- SO=SortOrder Optional sort order to output in. If not supplied OUTPUT is in the same order as INPUT.
- Default value: null. Possible values: {unsorted, queryname, coordinate}
+ SO=SortOrder Optional sort order to output in. If not supplied OUTPUT is in the same order as INPUT.
+ Default value: null. Possible values: {unsorted, queryname, coordinate}
RGID=String
- ID=String Read Group ID Default value: 1. This option can be set to 'null' to clear the default
- value.
+ ID=String Read Group ID Default value: 1. This option can be set to 'null' to clear the default
+ value.
RGLB=String
- LB=String Read Group Library Required.
-
+ LB=String Read Group Library Required.
+
RGPL=String
- PL=String Read Group platform (e.g. illumina, solid) Required.
+ PL=String Read Group platform (e.g. illumina, solid) Required.
RGPU=String
- PU=String Read Group platform unit (eg. run barcode) Required.
+ PU=String Read Group platform unit (eg. run barcode) Required.
RGSM=String
- SM=String Read Group sample name Required.
+ SM=String Read Group sample name Required.
RGCN=String
- CN=String Read Group sequencing center name Default value: null.
+ CN=String Read Group sequencing center name Default value: null.
RGDS=String
- DS=String Read Group description Default value: null.
+ DS=String Read Group description Default value: null.
RGDT=Iso8601Date
- DT=Iso8601Date Read Group run date Default value: null.
+ DT=Iso8601Date Read Group run date Default value: null.
RGPI=Integer
- PI=Integer Read Group predicted insert size Default value: null.
+ PI=Integer Read Group predicted insert size Default value: null.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_BedToIntervalList.xml
--- a/picard_BedToIntervalList.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_BedToIntervalList.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,40 +6,40 @@
-
+
-
+
@@ -54,11 +54,11 @@
-
+
-
+
@@ -74,8 +74,8 @@
-
-
+
+
.. class:: infomark
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CleanSam.xml
--- a/picard_CleanSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CleanSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,35 +6,36 @@
-
+
-
+
-
+
-
+
-
-
+
+
-
+
-
+
.. class:: infomark
@@ -45,9 +46,9 @@
1. to soft-clip an alignment that hangs off the end of its reference sequence.
2. to set MAPQ to 0 if a read is unmapped.
-
+
@dataset_collections@
-
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CollectAlignmentSummaryMetrics.xml
--- a/picard_CollectAlignmentSummaryMetrics.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CollectAlignmentSummaryMetrics.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,6 +6,7 @@
@@ -98,25 +99,25 @@
@description@
- MAX_INSERT_SIZE=Integer Paired end reads above this insert size will be considered chimeric along with
- inter-chromosomal pairs. Default value: 100000.
+ MAX_INSERT_SIZE=Integer Paired end reads above this insert size will be considered chimeric along with
+ inter-chromosomal pairs. Default value: 100000.
- ADAPTER_SEQUENCE=String List of adapter sequences to use when processing the alignment metrics This option may
- be specified 0 or more times.
+ ADAPTER_SEQUENCE=String List of adapter sequences to use when processing the alignment metrics This option may
+ be specified 0 or more times.
METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel
- LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
- LIBRARY, READ_GROUP} This option may be specified 0 or more times.
+ LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
+ LIBRARY, READ_GROUP} This option may be specified 0 or more times.
IS_BISULFITE_SEQUENCED=Boolean
- BS=Boolean Whether the SAM or BAM file consists of bisulfite sequenced reads.
+ BS=Boolean Whether the SAM or BAM file consists of bisulfite sequenced reads.
REFERENCE_SEQUENCE=File
- R=File Reference sequence fasta Default value: null.
+ R=File Reference sequence fasta Default value: null.
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored.
+ AS=Boolean If true (default), then the sort order in the header file will be ignored.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CollectBaseDistributionByCycle.xml
--- a/picard_CollectBaseDistributionByCycle.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CollectBaseDistributionByCycle.xml Tue Dec 06 10:04:41 2016 -0500
@@ -8,30 +8,31 @@
@@ -51,19 +52,19 @@
-
-
-
-
+
+
+
+
-
+
-
+
-
+
@@ -75,8 +76,8 @@
-
-
+
+
.. class:: infomark
@@ -89,19 +90,19 @@
@description@
- ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
- false. This option can be set to 'null' to clear the default value. Possible values:
- {true, false}
+ ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
+ false. This option can be set to 'null' to clear the default value. Possible values:
+ {true, false}
- PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
- This option can be set to 'null' to clear the default value. Possible values: {true,
- false}
+ PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
+ This option can be set to 'null' to clear the default value. Possible values: {true,
+ false}
REFERENCE_SEQUENCE=File
- R=File Reference sequence fasta Default value: null.
+ R=File Reference sequence fasta Default value: null.
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
+ AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CollectGcBiasMetrics.xml
--- a/picard_CollectGcBiasMetrics.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CollectGcBiasMetrics.xml Tue Dec 06 10:04:41 2016 -0500
@@ -8,6 +8,7 @@
@@ -67,16 +68,16 @@
-
+
-
+
-
+
-
+
@@ -90,8 +91,8 @@
-
-
+
+
.. class:: infomark
@@ -104,30 +105,30 @@
@description@
-
- DEVIATIONS=Double Generate mean, sd and plots by trimming the data down to MEDIAN +
- DEVIATIONS*MEDIAN_ABSOLUTE_DEVIATION. This is done because insert size data typically
- includes enough anomalous values from chimeras and other artifacts to make the mean and
- sd grossly misleading regarding the real distribution. Default value: 10.0.
+
+ DEVIATIONS=Double Generate mean, sd and plots by trimming the data down to MEDIAN +
+ DEVIATIONS*MEDIAN_ABSOLUTE_DEVIATION. This is done because insert size data typically
+ includes enough anomalous values from chimeras and other artifacts to make the mean and
+ sd grossly misleading regarding the real distribution. Default value: 10.0.
HISTOGRAM_WIDTH=Integer
- W=Integer Explicitly sets the Histogram width, overriding automatic truncation of Histogram tail.
- Also, when calculating mean and standard deviation, only bins <= Histogram_WIDTH will be
- included. Default value: not set.
+ W=Integer Explicitly sets the Histogram width, overriding automatic truncation of Histogram tail.
+ Also, when calculating mean and standard deviation, only bins <= Histogram_WIDTH will be
+ included. Default value: not set.
MINIMUM_PCT=Float
- M=Float When generating the Histogram, discard any data categories (out of FR, TANDEM, RF) that
- have fewer than this percentage of overall reads. (Range: 0 to 1). Default value: 0.05.
+ M=Float When generating the Histogram, discard any data categories (out of FR, TANDEM, RF) that
+ have fewer than this percentage of overall reads. (Range: 0 to 1). Default value: 0.05.
METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel
- LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
- LIBRARY, READ_GROUP} This option may be specified 0 or more times.
-
+ LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
+ LIBRARY, READ_GROUP} This option may be specified 0 or more times.
+
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
- value: true. This option can be set to 'null' to clear the default value. Possible
+ AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
+ value: true. This option can be set to 'null' to clear the default value. Possible
values: {true, false}
-
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CollectRnaSeqMetrics.xml
--- a/picard_CollectRnaSeqMetrics.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CollectRnaSeqMetrics.xml Tue Dec 06 10:04:41 2016 -0500
@@ -9,33 +9,33 @@
refFlat.tab &&
-
+
## Start picard command
-
+
@java_options@
picard
CollectRnaSeqMetrics
REF_FLAT=refFlat.tab
-
+
#if str( $ribosomal_intervals ) != "None":
RIBOSOMAL_INTERVALS="${ribosomal_intervals}"
#end if
-
+
STRAND_SPECIFICITY="${strand_specificity}"
MINIMUM_LENGTH="${minimum_length}"
CHART_OUTPUT="${pdfFile}"
@@ -43,20 +43,20 @@
#for $sequence_to_ignore in $ignore_list:
IGNORE_SEQUENCE="${sequence_to_ignore.sequence}"
#end for
-
+
RRNA_FRAGMENT_PERCENTAGE="${rrna_fragment_percentage}"
METRIC_ACCUMULATION_LEVEL="${metric_accumulation_level}"
- INPUT="${inputFile}"
+ INPUT='$inputFile.element_identifier'
OUTPUT="${outFile}"
REFERENCE_SEQUENCE="${reference_fasta_filename}"
ASSUME_SORTED="${assume_sorted}"
-
+
QUIET=true
VERBOSITY=ERROR
VALIDATION_STRINGENCY=${validation_stringency}
-
+
]]>
-
+
@@ -73,7 +73,7 @@
-
+
@@ -93,7 +93,7 @@
-
+
@@ -101,7 +101,7 @@
-
+
@@ -156,41 +156,41 @@
8. Click **Send query to Galaxy**
9. A new dataset will appear in the current Galaxy history
10. Use this dataset as the input for **Gene annotations in refFlat form** dropdown of this tool
-
+
.. _refFlat: http://genome.ucsc.edu/goldenPath/gbdDescriptionsOld.html#RefFlat
@description@
- REF_FLAT=File Gene annotations in refFlat form. Format described here:
- http://genome.ucsc.edu/goldenPath/gbdDescriptionsOld.html#RefFlat Required.
+ REF_FLAT=File Gene annotations in refFlat form. Format described here:
+ http://genome.ucsc.edu/goldenPath/gbdDescriptionsOld.html#RefFlat Required.
- RIBOSOMAL_INTERVALS=File Location of rRNA sequences in genome, in interval_list format. If not specified no bases
- will be identified as being ribosomal. Format described here:
+ RIBOSOMAL_INTERVALS=File Location of rRNA sequences in genome, in interval_list format. If not specified no bases
+ will be identified as being ribosomal. Format described here:
http://picard.sourceforge.net/javadoc/net/sf/picard/util/IntervalList.html and can be
generated from BED datasetes using Galaxy's wrapper for picard_BedToIntervalList tool
STRAND_SPECIFICITY=StrandSpecificity
- STRAND=StrandSpecificity For strand-specific library prep. For unpaired reads, use FIRST_READ_TRANSCRIPTION_STRAND
- if the reads are expected to be on the transcription strand. Required. Possible values:
- {NONE, FIRST_READ_TRANSCRIPTION_STRAND, SECOND_READ_TRANSCRIPTION_STRAND}
+ STRAND=StrandSpecificity For strand-specific library prep. For unpaired reads, use FIRST_READ_TRANSCRIPTION_STRAND
+ if the reads are expected to be on the transcription strand. Required. Possible values:
+ {NONE, FIRST_READ_TRANSCRIPTION_STRAND, SECOND_READ_TRANSCRIPTION_STRAND}
- MINIMUM_LENGTH=Integer When calculating coverage based values (e.g. CV of coverage) only use transcripts of this
+ MINIMUM_LENGTH=Integer When calculating coverage based values (e.g. CV of coverage) only use transcripts of this
length or greater. Default value: 500.
- IGNORE_SEQUENCE=String If a read maps to a sequence specified with this option, all the bases in the read are
- counted as ignored bases.
+ IGNORE_SEQUENCE=String If a read maps to a sequence specified with this option, all the bases in the read are
+ counted as ignored bases.
RRNA_FRAGMENT_PERCENTAGE=Double
- This percentage of the length of a fragment must overlap one of the ribosomal intervals
- for a read or read pair by this must in order to be considered rRNA. Default value: 0.8.
+ This percentage of the length of a fragment must overlap one of the ribosomal intervals
+ for a read or read pair by this must in order to be considered rRNA. Default value: 0.8.
METRIC_ACCUMULATION_LEVEL=MetricAccumulationLevel
- LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
+ LEVEL=MetricAccumulationLevel The level(s) at which to accumulate metrics. Possible values: {ALL_READS, SAMPLE,
LIBRARY, READ_GROUP} This option may be specified 0 or more times.
-
+
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
- value: true. Possible values: {true, false}
+ AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
+ value: true. Possible values: {true, false}
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_CollectWgsMetrics.xml
--- a/picard_CollectWgsMetrics.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_CollectWgsMetrics.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,29 +6,30 @@
@@ -52,15 +53,15 @@
-
+
-
+
-
+
-
+
@@ -70,10 +71,10 @@
-
+
-
-
+
+
.. class:: infomark
@@ -87,14 +88,14 @@
@description@
MINIMUM_MAPPING_QUALITY=Integer
- MQ=Integer Minimum mapping quality for a read to contribute coverage. Default value: 20.
+ MQ=Integer Minimum mapping quality for a read to contribute coverage. Default value: 20.
MINIMUM_BASE_QUALITY=Integer
- Q=Integer Minimum base quality for a base to contribute coverage. Default value: 20.
+ Q=Integer Minimum base quality for a base to contribute coverage. Default value: 20.
COVERAGE_CAP=Integer
- CAP=Integer Treat bases with coverage exceeding this value as if they had coverage at this value.
- Default value: 250.
+ CAP=Integer Treat bases with coverage exceeding this value as if they had coverage at this value.
+ Default value: 250.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_DownsampleSam.xml
--- a/picard_DownsampleSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_DownsampleSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,9 +6,10 @@
-
+
-
+
-
-
+
+
-
+
@@ -52,18 +53,18 @@
@description@
INPUT=File
- I=File The input SAM or BAM file to downsample. Required.
+ I=File The input SAM or BAM file to downsample. Required.
OUTPUT=File
- O=File The output, downsampled, SAM or BAM file to write. Required.
+ O=File The output, downsampled, SAM or BAM file to write. Required.
RANDOM_SEED=Long
R=Long Random seed to use if reproducibilty is desired. Setting to null will cause multiple
invocations to produce different results.
-
+
PROBABILITY=Double
- P=Double The probability of keeping any individual read, between 0 and 1.
-
+ P=Double The probability of keeping any individual read, between 0 and 1.
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_EstimateLibraryComplexity.xml
--- a/picard_EstimateLibraryComplexity.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_EstimateLibraryComplexity.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,13 +6,13 @@
@@ -42,13 +42,13 @@
-
-
-
+
+
+
-
+
@@ -62,8 +62,8 @@
-
-
+
+
**Purpose**
@@ -86,40 +86,40 @@
@description@
- MIN_IDENTICAL_BASES=Integer The minimum number of bases at the starts of reads that must be identical for reads to be
- grouped together for duplicate detection. In effect total_reads / 4^max_id_bases reads
- will be compared at a time, so lower numbers will produce more accurate results but
- consume exponentially more memory and CPU. Default value: 5.
-
- MAX_DIFF_RATE=Double The maximum rate of differences between two reads to call them identical. Default value:
+ MIN_IDENTICAL_BASES=Integer The minimum number of bases at the starts of reads that must be identical for reads to be
+ grouped together for duplicate detection. In effect total_reads / 4^max_id_bases reads
+ will be compared at a time, so lower numbers will produce more accurate results but
+ consume exponentially more memory and CPU. Default value: 5.
+
+ MAX_DIFF_RATE=Double The maximum rate of differences between two reads to call them identical. Default value:
0.03.
-
- MIN_MEAN_QUALITY=Integer The minimum mean quality of the bases in a read pair for the read to be analyzed. Reads
- with lower average quality are filtered out and not considered in any calculations.
+
+ MIN_MEAN_QUALITY=Integer The minimum mean quality of the bases in a read pair for the read to be analyzed. Reads
+ with lower average quality are filtered out and not considered in any calculations.
Default value: 20.
-
- MAX_GROUP_RATIO=Integer Do not process self-similar groups that are this many times over the mean expected group
- size. I.e. if the input contains 10m read pairs and MIN_IDENTICAL_BASES is set to 5, then
+
+ MAX_GROUP_RATIO=Integer Do not process self-similar groups that are this many times over the mean expected group
+ size. I.e. if the input contains 10m read pairs and MIN_IDENTICAL_BASES is set to 5, then
the mean expected group size would be approximately 10 reads. Default value: 500.
-
- READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read
- names are parsed to extract three variables: tile/region, x coordinate and y coordinate.
- These values are used to estimate the rate of optical duplication in order to give a more
- accurate estimated library size. Set this option to null to disable optical duplicate
- detection. The regular expression should contain three capture groups for the three
- variables, in order. It must match the entire read name. Note that if the default regex
- is specified, a regex match is not actually done, but instead the read name is split on
- colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be
- tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements
- are assumed to be tile, x and y values. Default value:
+
+ READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read
+ names are parsed to extract three variables: tile/region, x coordinate and y coordinate.
+ These values are used to estimate the rate of optical duplication in order to give a more
+ accurate estimated library size. Set this option to null to disable optical duplicate
+ detection. The regular expression should contain three capture groups for the three
+ variables, in order. It must match the entire read name. Note that if the default regex
+ is specified, a regex match is not actually done, but instead the read name is split on
+ colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be
+ tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements
+ are assumed to be tile, x and y values. Default value:
[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*.
-
- OPTICAL_DUPLICATE_PIXEL_DISTANCE=Integer
- The maximum offset between two duplicte clusters in order to consider them optical
- duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels)
- unless using later versions of the Illumina pipeline that multiply pixel values by 10, in
+
+ OPTICAL_DUPLICATE_PIXEL_DISTANCE=Integer
+ The maximum offset between two duplicte clusters in order to consider them optical
+ duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels)
+ unless using later versions of the Illumina pipeline that multiply pixel values by 10, in
which case 50-100 is more normal. Default value: 100.
-
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_FastqToSam.xml
--- a/picard_FastqToSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_FastqToSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,10 +6,9 @@
@@ -86,7 +85,7 @@
-
+
@@ -108,15 +107,15 @@
-
+
-
-
-
+
+
+
-
+
@@ -139,9 +138,9 @@
-
+
-
+
.. class:: infomark
@@ -157,62 +156,62 @@
@description@
FASTQ=File
- F1=File Input fastq file for single end data, or first read in paired end
+ F1=File Input fastq file for single end data, or first read in paired end
data. Required.
-
+
FASTQ2=File
- F2=File Input fastq file for the second read of paired end data (if used).
+ F2=File Input fastq file for the second read of paired end data (if used).
QUALITY_FORMAT=FastqQualityFormat
- V=FastqQualityFormat A value describing how the quality values are encoded in the fastq. Either Solexa for
- pre-pipeline 1.3 style scores (solexa scaling + 66), Illumina for pipeline 1.3 and above
- (phred scaling + 64) or Standard for phred scaled scores with a character shift of 33.
- If this value is not specified, the quality format will be detected automatically.
- Default value: null. Possible values: {Solexa, Illumina, Standard}
+ V=FastqQualityFormat A value describing how the quality values are encoded in the fastq. Either Solexa for
+ pre-pipeline 1.3 style scores (solexa scaling + 66), Illumina for pipeline 1.3 and above
+ (phred scaling + 64) or Standard for phred scaled scores with a character shift of 33.
+ If this value is not specified, the quality format will be detected automatically.
+ Default value: null. Possible values: {Solexa, Illumina, Standard}
READ_GROUP_NAME=String
- RG=String Read group name Default value: A.
-
+ RG=String Read group name Default value: A.
+
SAMPLE_NAME=String
- SM=String Sample name to insert into the read group header Required.
-
+ SM=String Sample name to insert into the read group header Required.
+
LIBRARY_NAME=String
LB=String The library name to place into the LB attribute in the read group header.
-
+
PLATFORM_UNIT=String
PU=String The platform unit (often run_barcode.lane) to insert into the read group header.
-
+
PLATFORM=String
PL=String The platform type (e.g. illumina, solid) to insert into the read group header.
-
+
SEQUENCING_CENTER=String
CN=String The sequencing center from which the data originated.
-
+
PREDICTED_INSERT_SIZE=Integer
PI=Integer Predicted median insert size, to insert into the read group header.
-
+
COMMENT=String
- CO=String Comment to include in the merged output file's header.
-
+ CO=String Comment to include in the merged output file's header.
+
DESCRIPTION=String
- DS=String Inserted into the read group header.
-
+ DS=String Inserted into the read group header.
+
RUN_DATE=Iso8601Date
- DT=Iso8601Date Date the run was produced, to insert into the read group header.
-
- MIN_Q=Integer Minimum quality allowed in the input fastq. An exception will be thrown if a quality is
+ DT=Iso8601Date Date the run was produced, to insert into the read group header.
+
+ MIN_Q=Integer Minimum quality allowed in the input fastq. An exception will be thrown if a quality is
less than this value. Default value: 0.
-
- MAX_Q=Integer Maximum quality allowed in the input fastq. An exception will be thrown if a quality is
+
+ MAX_Q=Integer Maximum quality allowed in the input fastq. An exception will be thrown if a quality is
greater than this value. Default value: 93.
-
+
STRIP_UNPAIRED_MATE_NUMBER=Boolean
- If true and this is an unpaired fastq any occurance of '/1' will be removed from the end
- of a read name. Default value: false. Possible values: {true, false}
-
+ If true and this is an unpaired fastq any occurance of '/1' will be removed from the end
+ of a read name. Default value: false. Possible values: {true, false}
+
ALLOW_AND_IGNORE_EMPTY_LINES=Boolean
- Allow (and ignore) empty lines Default value: false. Possible values: {true, false}
-
+ Allow (and ignore) empty lines Default value: false. Possible values: {true, false}
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_FilterSamReads.xml
--- a/picard_FilterSamReads.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_FilterSamReads.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,35 +6,35 @@
@@ -54,15 +54,15 @@
-
+
-
-
-
+
+
+
-
+
@@ -79,8 +79,8 @@
-
-
+
+
**Purpose**
@@ -93,32 +93,32 @@
**Warning on using this tool on BWA-MEM output**
-This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts.
+This tool will likely fail on BAM datasets generated by BWA MEM as it generates partial read alignemnts.
@dataset_collections@
@description@
FILTER=Filter Filter. Required. Possible values:
- includeAligned [OUTPUT SAM/BAM will contain aligned
- reads only. (Note that *both* first and
+ includeAligned [OUTPUT SAM/BAM will contain aligned
+ reads only. (Note that *both* first and
second of paired reads must be aligned to be included
- in the OUTPUT SAM or BAM)],
-
+ in the OUTPUT SAM or BAM)],
+
excludeAligned [OUTPUT SAM/BAM will contain un-mapped reads only.
- (Note that *both* first and second of pair must be aligned to be
+ (Note that *both* first and second of pair must be aligned to be
excluded from the OUTPUT SAM or BAM)]
-
- includeReadList [OUTPUT SAM/BAM will contain reads
+
+ includeReadList [OUTPUT SAM/BAM will contain reads
that are supplied in the READ_LIST_FILE file]
-
- excludeReadList [OUTPUT bam will contain
- reads that are *not* supplied in the READ_LIST_FILE file]}
+
+ excludeReadList [OUTPUT bam will contain
+ reads that are *not* supplied in the READ_LIST_FILE file]}
READ_LIST_FILE=File
- RLF=File Read List File containing reads that will be included or excluded from the OUTPUT SAM or
- BAM file. Default value: null.
-
+ RLF=File Read List File containing reads that will be included or excluded from the OUTPUT SAM or
+ BAM file. Default value: null.
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_FixMateInformation.xml
--- a/picard_FixMateInformation.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_FixMateInformation.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,33 +6,32 @@
-
+
-
-
-
+
+
+
-
+
@@ -42,8 +41,8 @@
-
-
+
+
**Purpose**
@@ -64,12 +63,12 @@
@description@
ASSUME_SORTED=Boolean
- AS=Boolean If true, assume that the input file is queryname sorted, even if the header says
- otherwise. Default value: false.
-
+ AS=Boolean If true, assume that the input file is queryname sorted, even if the header says
+ otherwise. Default value: false.
+
ADD_MATE_CIGAR=Boolean
- MC=Boolean Adds the mate CIGAR tag (MC) if true, does not if false. Default value: true.
-
+ MC=Boolean Adds the mate CIGAR tag (MC) if true, does not if false. Default value: true.
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_MarkDuplicates.xml
--- a/picard_MarkDuplicates.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_MarkDuplicates.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,11 +6,11 @@
-
+
@@ -60,14 +60,14 @@
-
-
-
+
+
+
-
+
@@ -83,8 +83,8 @@
-
-
+
+
**Purpose**
@@ -110,44 +110,44 @@
@description@
- MINIMUM_DISTANCE=Integer The minimum distance to buffer records to account for clipping on the 5' end of the
- records.Set this number to -1 to use twice the first read's read length (or 100,
- whichever is smaller). Default value: -1. This option can be set to 'null' to clear the
- default value.
-
+ MINIMUM_DISTANCE=Integer The minimum distance to buffer records to account for clipping on the 5' end of the
+ records.Set this number to -1 to use twice the first read's read length (or 100,
+ whichever is smaller). Default value: -1. This option can be set to 'null' to clear the
+ default value.
+
SKIP_PAIRS_WITH_NO_MATE_CIGAR=Boolean
- Skip record pairs with no mate cigar and include them in the output. Default value:
- true. This option can be set to 'null' to clear the default value. Possible values:
- {true, false}
+ Skip record pairs with no mate cigar and include them in the output. Default value:
+ true. This option can be set to 'null' to clear the default value. Possible values:
+ {true, false}
- COMMENT=String
- CO=String Comment(s) to include in the output file's header. This option may be specified 0 or
- more times.
+ COMMENT=String
+ CO=String Comment(s) to include in the output file's header. This option may be specified 0 or
+ more times.
+
+ REMOVE_DUPLICATES=Boolean If true do not write duplicates to the output file instead of writing them with
+ appropriate flags set. Default value: false.
- REMOVE_DUPLICATES=Boolean If true do not write duplicates to the output file instead of writing them with
- appropriate flags set. Default value: false.
-
- READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read
- names are parsed to extract three variables: tile/region, x coordinate and y coordinate.
- These values are used to estimate the rate of optical duplication in order to give a more
- accurate estimated library size. Set this option to null to disable optical duplicate
- detection. The regular expression should contain three capture groups for the three
- variables, in order. It must match the entire read name. Note that if the default regex
- is specified, a regex match is not actually done, but instead the read name is split on
- colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be
- tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements
- are assumed to be tile, x and y values. Default value:
+ READ_NAME_REGEX=String Regular expression that can be used to parse read names in the incoming SAM file. Read
+ names are parsed to extract three variables: tile/region, x coordinate and y coordinate.
+ These values are used to estimate the rate of optical duplication in order to give a more
+ accurate estimated library size. Set this option to null to disable optical duplicate
+ detection. The regular expression should contain three capture groups for the three
+ variables, in order. It must match the entire read name. Note that if the default regex
+ is specified, a regex match is not actually done, but instead the read name is split on
+ colon character. For 5 element names, the 3rd, 4th and 5th elements are assumed to be
+ tile, x and y values. For 7 element names (CASAVA 1.8), the 5th, 6th, and 7th elements
+ are assumed to be tile, x and y values. Default value:
[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*.
-
+
DUPLICATE_SCORING_STRATEGY=ScoringStrategy
- DS=ScoringStrategy The scoring strategy for choosing the non-duplicate among candidates. Default value:
- TOTAL_MAPPED_REFERENCE_LENGTH. Possible values: {SUM_OF_BASE_QUALITIES, TOTAL_MAPPED_REFERENCE_LENGTH}
-
+ DS=ScoringStrategy The scoring strategy for choosing the non-duplicate among candidates. Default value:
+ TOTAL_MAPPED_REFERENCE_LENGTH. Possible values: {SUM_OF_BASE_QUALITIES, TOTAL_MAPPED_REFERENCE_LENGTH}
+
OPTICAL_DUPLICATE_PIXEL_DISTANCE=Integer
- The maximum offset between two duplicte clusters in order to consider them optical
- duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels)
- unless using later versions of the Illumina pipeline that multiply pixel values by 10, in
- which case 50-100 is more normal. Default value: 100.
+ The maximum offset between two duplicte clusters in order to consider them optical
+ duplicates. This should usually be set to some fairly small number (e.g. 5-10 pixels)
+ unless using later versions of the Illumina pipeline that multiply pixel values by 10, in
+ which case 50-100 is more normal. Default value: 100.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_MeanQualityByCycle.xml
--- a/picard_MeanQualityByCycle.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_MeanQualityByCycle.xml Tue Dec 06 10:04:41 2016 -0500
@@ -9,30 +9,30 @@
@@ -55,16 +55,16 @@
-
+
-
+
-
+
-
+
@@ -74,31 +74,31 @@
-
+
-
-
+
+
.. class:: infomark
**Purpose**
-Program to chart the distribution of base qualities by cycle within reads supplied in a SAM or BAM dataset.
+Program to chart the distribution of base qualities by cycle within reads supplied in a SAM or BAM dataset.
@dataset_collections@
@description@
- ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
- false. Possible values: {true, false}
+ ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
+ false. Possible values: {true, false}
- PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
- This option can be set to 'null' to clear the default value. Possible values: {true,
- false}
+ PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
+ This option can be set to 'null' to clear the default value. Possible values: {true,
+ false}
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored. Default: True
+ AS=Boolean If true (default), then the sort order in the header file will be ignored. Default: True
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_MergeBamAlignment.xml
--- a/picard_MergeBamAlignment.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_MergeBamAlignment.xml Tue Dec 06 10:04:41 2016 -0500
@@ -8,29 +8,29 @@
@java_options@
#set $picard_dict = "localref.dict"
#set $ref_fasta = "localref.fa" ## This is done because picards "likes" .fa extension
-
+
ln -s "${reference_source.ref_file}" "${ref_fasta}" &&
-
+
#if str( $reference_source.reference_source_selector ) == "history":
-
+
picard CreateSequenceDictionary REFERENCE="${ref_fasta}" OUTPUT="${picard_dict}"
QUIET=true
VERBOSITY=ERROR
-
+
&&
-
+
#else:
-
+
#set $ref_fasta = str( $reference_source.ref_file.fields.path )
-
+
#end if
-
+
picard
MergeBamAlignment
UNMAPPED_BAM="${unmapped_bam}"
-
+
PAIRED_RUN=true ##This argument is ignored and will be removed. Required. Possible values: {true, false}
-
+
#if str( $aligned_or_read1_and_read2.aligned_or_read1_and_read2_selector ) == "paired_one_file":
#for $dataset in $aligned_or_read1_and_read2.aligned_bams:
ALIGNED_BAM="${dataset.aligned_bam}"
@@ -47,48 +47,48 @@
READ1_ALIGNED_BAM="${dataset.read1_aligned_bam}"
#end for
#end if
-
+
OUTPUT="${outFile}"
REFERENCE_SEQUENCE="${ref_fasta}"
-
+
CLIP_ADAPTERS="${clip_adapters}"
IS_BISULFITE_SEQUENCE="${is_bisulfite_sequence}"
ALIGNED_READS_ONLY="${aligned_reads_only}"
MAX_INSERTIONS_OR_DELETIONS="${max_insertions_or_deletions}"
-
+
#for $attribute in $attributes_to_retain:
ATTRIBUTES_TO_RETAIN="${$attribute.attribute}"
#end for
-
+
#for $attribute in $attributes_to_remove:
ATTRIBUTES_TO_REMOVE="${$attribute.attribute}"
#end for
-
+
READ1_TRIM="${read1_trim}"
READ2_TRIM="${read2_trim}"
-
+
#if str( $orientations ) != "None":
#for $orientation in str( $orientations ).split(','): ## See trello card https://trello.com/c/9nW02Zhd
EXPECTED_ORIENTATIONS="${orientation}"
#end for
#end if
-
- ALIGNER_PROPER_PAIR_FLAGS="${aligner_proper_pair_flags}"
+
+ ALIGNER_PROPER_PAIR_FLAGS="${aligner_proper_pair_flags}"
PRIMARY_ALIGNMENT_STRATEGY="${primary_alignment_strategy}"
CLIP_OVERLAPPING_READS="${clip_overlapping_reads}"
INCLUDE_SECONDARY_ALIGNMENTS="${include_secondary_alignments}"
ADD_MATE_CIGAR="${add_mate_cigar}"
-
+
VALIDATION_STRINGENCY="${validation_stringency}"
SORT_ORDER=coordinate
QUIET=true
VERBOSITY=ERROR
-
+
]]>
-
+
-
+
@@ -103,11 +103,11 @@
-
+
-
+
@@ -134,39 +134,39 @@
-
+
-
+
-
+
-
+
-
+
-
-
+
+
-
+
-
+
@@ -197,8 +197,8 @@
-
-
+
+
.. class:: infomark
@@ -212,96 +212,96 @@
@description@
UNMAPPED_BAM=File
- UNMAPPED=File Original SAM or BAM file of unmapped reads, which must be in queryname order. Required.
-
+ UNMAPPED=File Original SAM or BAM file of unmapped reads, which must be in queryname order. Required.
+
ALIGNED_BAM=File
- ALIGNED=File SAM or BAM file(s) with alignment data. This option may be specified 0 or more times.
- Cannot be used in conjuction with option(s) READ1_ALIGNED_BAM (R1_ALIGNED)
+ ALIGNED=File SAM or BAM file(s) with alignment data. This option may be specified 0 or more times.
+ Cannot be used in conjuction with option(s) READ1_ALIGNED_BAM (R1_ALIGNED)
READ2_ALIGNED_BAM (R2_ALIGNED)
-
+
READ1_ALIGNED_BAM=File
- R1_ALIGNED=File SAM or BAM file(s) with alignment data from the first read of a pair. This option may be
- specified 0 or more times. Cannot be used in conjuction with option(s) ALIGNED_BAM
+ R1_ALIGNED=File SAM or BAM file(s) with alignment data from the first read of a pair. This option may be
+ specified 0 or more times. Cannot be used in conjuction with option(s) ALIGNED_BAM
(ALIGNED)
-
+
READ2_ALIGNED_BAM=File
- R2_ALIGNED=File SAM or BAM file(s) with alignment data from the second read of a pair. This option may
- be specified 0 or more times. Cannot be used in conjuction with option(s) ALIGNED_BAM
+ R2_ALIGNED=File SAM or BAM file(s) with alignment data from the second read of a pair. This option may
+ be specified 0 or more times. Cannot be used in conjuction with option(s) ALIGNED_BAM
(ALIGNED)
-
+
PAIRED_RUN=Boolean
- PE=Boolean This argument is ignored and will be removed. Required. Possible values: {true, false}
-
+ PE=Boolean This argument is ignored and will be removed. Required. Possible values: {true, false}
+
JUMP_SIZE=Integer
- JUMP=Integer The expected jump size (required if this is a jumping library). Deprecated. Use
- EXPECTED_ORIENTATIONS instead Default value: null. Cannot be used in conjuction with
+ JUMP=Integer The expected jump size (required if this is a jumping library). Deprecated. Use
+ EXPECTED_ORIENTATIONS instead Default value: null. Cannot be used in conjuction with
option(s) EXPECTED_ORIENTATIONS (ORIENTATIONS)
-
- CLIP_ADAPTERS=Boolean Whether to clip adapters where identified. Default value: true. Possible values: {true, false}
-
- IS_BISULFITE_SEQUENCE=Boolean Whether the lane is bisulfite sequence (used when caculating the NM tag). Default value:
- false. Possible values: {true, false}
-
- ALIGNED_READS_ONLY=Boolean Whether to output only aligned reads. Default value: false. Possible values: {true, false}
-
+
+ CLIP_ADAPTERS=Boolean Whether to clip adapters where identified. Default value: true. Possible values: {true, false}
+
+ IS_BISULFITE_SEQUENCE=Boolean Whether the lane is bisulfite sequence (used when caculating the NM tag). Default value:
+ false. Possible values: {true, false}
+
+ ALIGNED_READS_ONLY=Boolean Whether to output only aligned reads. Default value: false. Possible values: {true, false}
+
MAX_INSERTIONS_OR_DELETIONS=Integer
- MAX_GAPS=Integer The maximum number of insertions or deletions permitted for an alignment to be included.
- Alignments with more than this many insertions or deletions will be ignored. Set to -1 to
+ MAX_GAPS=Integer The maximum number of insertions or deletions permitted for an alignment to be included.
+ Alignments with more than this many insertions or deletions will be ignored. Set to -1 to
allow any number of insertions or deletions. Default value: 1.
-
- ATTRIBUTES_TO_RETAIN=String Reserved alignment attributes (tags starting with X, Y, or Z) that should be brought over
- from the alignment data when merging. This option may be specified 0 or more times.
-
- ATTRIBUTES_TO_REMOVE=String Attributes from the alignment record that should be removed when merging. This overrides
- ATTRIBUTES_TO_RETAIN if they share common tags. This option may be specified 0 or more
- times.
-
+
+ ATTRIBUTES_TO_RETAIN=String Reserved alignment attributes (tags starting with X, Y, or Z) that should be brought over
+ from the alignment data when merging. This option may be specified 0 or more times.
+
+ ATTRIBUTES_TO_REMOVE=String Attributes from the alignment record that should be removed when merging. This overrides
+ ATTRIBUTES_TO_RETAIN if they share common tags. This option may be specified 0 or more
+ times.
+
READ1_TRIM=Integer
- R1_TRIM=Integer The number of bases trimmed from the beginning of read 1 prior to alignment Default
- value: 0.
-
+ R1_TRIM=Integer The number of bases trimmed from the beginning of read 1 prior to alignment Default
+ value: 0.
+
READ2_TRIM=Integer
- R2_TRIM=Integer The number of bases trimmed from the beginning of read 2 prior to alignment Default
- value: 0.
-
+ R2_TRIM=Integer The number of bases trimmed from the beginning of read 2 prior to alignment Default
+ value: 0.
+
EXPECTED_ORIENTATIONS=PairOrientation
- ORIENTATIONS=PairOrientation The expected orientation of proper read pairs. Replaces JUMP_SIZE Possible values: {FR,
- RF, TANDEM} This option may be specified 0 or more times. Cannot be used in conjuction
+ ORIENTATIONS=PairOrientation The expected orientation of proper read pairs. Replaces JUMP_SIZE Possible values: {FR,
+ RF, TANDEM} This option may be specified 0 or more times. Cannot be used in conjuction
with option(s) JUMP_SIZE (JUMP)
-
+
ALIGNER_PROPER_PAIR_FLAGS=Boolean
- Use the aligner's idea of what a proper pair is rather than computing in this program.
- Default value: false. Possible values: {true, false}
-
+ Use the aligner's idea of what a proper pair is rather than computing in this program.
+ Default value: false. Possible values: {true, false}
+
SORT_ORDER=SortOrder
SO=SortOrder The order in which the merged reads should be output. Default value: coordinate.
- Possible values: {unsorted, queryname, coordinate}
-
+ Possible values: {unsorted, queryname, coordinate}
+
PRIMARY_ALIGNMENT_STRATEGY=PrimaryAlignmentStrategy
- Strategy for selecting primary alignment when the aligner has provided more than one
- alignment for a pair or fragment, and none are marked as primary, more than one is marked
- as primary, or the primary alignment is filtered out for some reason. BestMapq expects
- that multiple alignments will be correlated with HI tag, and prefers the pair of
- alignments with the largest MAPQ, in the absence of a primary selected by the aligner.
- EarliestFragment prefers the alignment which maps the earliest base in the read. Note
- that EarliestFragment may not be used for paired reads. BestEndMapq is appropriate for
- cases in which the aligner is not pair-aware, and does not output the HI tag. It simply
- picks the alignment for each end with the highest MAPQ, and makes those alignments
- primary, regardless of whether the two alignments make sense together.MostDistant is also
- for a non-pair-aware aligner, and picks the alignment pair with the largest insert size.
- If all alignments would be chimeric, it picks the alignments for each end with the best
+ Strategy for selecting primary alignment when the aligner has provided more than one
+ alignment for a pair or fragment, and none are marked as primary, more than one is marked
+ as primary, or the primary alignment is filtered out for some reason. BestMapq expects
+ that multiple alignments will be correlated with HI tag, and prefers the pair of
+ alignments with the largest MAPQ, in the absence of a primary selected by the aligner.
+ EarliestFragment prefers the alignment which maps the earliest base in the read. Note
+ that EarliestFragment may not be used for paired reads. BestEndMapq is appropriate for
+ cases in which the aligner is not pair-aware, and does not output the HI tag. It simply
+ picks the alignment for each end with the highest MAPQ, and makes those alignments
+ primary, regardless of whether the two alignments make sense together.MostDistant is also
+ for a non-pair-aware aligner, and picks the alignment pair with the largest insert size.
+ If all alignments would be chimeric, it picks the alignments for each end with the best
MAPQ. For all algorithms, ties are resolved arbitrarily. Default value: BestMapq.
- Possible values: {BestMapq, EarliestFragment, BestEndMapq, MostDistant}
-
- CLIP_OVERLAPPING_READS=BooleanFor paired reads, soft clip the 3' end of each read if necessary so that it does not
- extend past the 5' end of its mate. Default value: true. Possible values: {true, false}
-
+ Possible values: {BestMapq, EarliestFragment, BestEndMapq, MostDistant}
+
+ CLIP_OVERLAPPING_READS=BooleanFor paired reads, soft clip the 3' end of each read if necessary so that it does not
+ extend past the 5' end of its mate. Default value: true. Possible values: {true, false}
+
INCLUDE_SECONDARY_ALIGNMENTS=Boolean
If false, do not write secondary alignments to output. Default value: true.
- Possible values: {true, false}
-
+ Possible values: {true, false}
+
ADD_MATE_CIGAR=Boolean
- MC=Boolean Adds the mate CIGAR tag (MC) if true, does not if false. Possible values: {true, false}
+ MC=Boolean Adds the mate CIGAR tag (MC) if true, does not if false. Possible values: {true, false}
diff -r 05087b27692a -r 7e6fd3d0f16e picard_MergeSamFiles.xml
--- a/picard_MergeSamFiles.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_MergeSamFiles.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,17 +6,16 @@
@@ -37,15 +36,15 @@
-
+
-
-
-
+
+
+
-
+
@@ -55,8 +54,8 @@
-
-
+
+
**Purpose**
@@ -68,17 +67,17 @@
@description@
ASSUME_SORTED=Boolean
- AS=Boolean If true, assume that the input files are in the same sort order as the requested output
- sort order, even if their headers say otherwise. Default value: false. This option can
- be set to 'null' to clear the default value. Possible values: {true, false}
-
+ AS=Boolean If true, assume that the input files are in the same sort order as the requested output
+ sort order, even if their headers say otherwise. Default value: false. This option can
+ be set to 'null' to clear the default value. Possible values: {true, false}
+
MERGE_SEQUENCE_DICTIONARIES=Boolean
- MSD=Boolean Merge the sequence dictionaries Default value: false. This option can be set to 'null'
- to clear the default value. Possible values: {true, false}
-
+ MSD=Boolean Merge the sequence dictionaries Default value: false. This option can be set to 'null'
+ to clear the default value. Possible values: {true, false}
+
COMMENT=String
- CO=String Comment(s) to include in the merged output file's header. This option may be specified 0
- or more times.
+ CO=String Comment(s) to include in the merged output file's header. This option may be specified 0
+ or more times.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_NormalizeFasta.xml
--- a/picard_NormalizeFasta.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_NormalizeFasta.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,34 +6,32 @@
-
-
+
+
-
+
@@ -42,8 +40,8 @@
-
-
+
+
**Purpose**
@@ -54,10 +52,10 @@
@description@
- LINE_LENGTH=Integer The line length to be used for the output fasta file. Default value: 100.
-
+ LINE_LENGTH=Integer The line length to be used for the output fasta file. Default value: 100.
+
TRUNCATE_SEQUENCE_NAMES_AT_WHITESPACE=Boolean
- Truncate sequence names at first whitespace. Default value: false. Possible values: {true, false}
+ Truncate sequence names at first whitespace. Default value: false. Possible values: {true, false}
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_QualityScoreDistribution.xml
--- a/picard_QualityScoreDistribution.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_QualityScoreDistribution.xml Tue Dec 06 10:04:41 2016 -0500
@@ -9,31 +9,31 @@
@@ -57,16 +57,16 @@
-
+
-
+
-
+
-
+
@@ -77,10 +77,10 @@
-
+
-
-
+
+
.. class:: infomark
@@ -93,17 +93,17 @@
@description@
- ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
- false. Possible values: {true, false}
+ ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
+ false. Possible values: {true, false}
- PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
+ PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
Possible values: {true, false}
-
- INCLUDE_NO_CALLS=Boolean If set to true, include quality for no-call bases in the distribution. Default value:
- false. Possible values: {true, false}
+
+ INCLUDE_NO_CALLS=Boolean If set to true, include quality for no-call bases in the distribution. Default value:
+ false. Possible values: {true, false}
ASSUME_SORTED=Boolean
- AS=Boolean If true (default), then the sort order in the header file will be ignored. Default: True
+ AS=Boolean If true (default), then the sort order in the header file will be ignored. Default: True
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_ReorderSam.xml
--- a/picard_ReorderSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_ReorderSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,41 +6,42 @@
-
+
-
+
@@ -55,7 +56,7 @@
-
+
@@ -64,7 +65,7 @@
-
+
@@ -79,8 +80,8 @@
-
-
+
+
.. class:: infomark
@@ -100,14 +101,14 @@
@description@
ALLOW_INCOMPLETE_DICT_CONCORDANCE=Boolean
- S=Boolean If true, then allows only a partial overlap of the BAM contigs with the new reference
- sequence contigs. By default, this tool requires a corresponding contig in the new
- reference for each read contig Default value: false. Possible values: {true, false}
-
+ S=Boolean If true, then allows only a partial overlap of the BAM contigs with the new reference
+ sequence contigs. By default, this tool requires a corresponding contig in the new
+ reference for each read contig Default value: false. Possible values: {true, false}
+
ALLOW_CONTIG_LENGTH_DISCORDANCE=Boolean
- U=Boolean If true, then permits mapping from a read contig to a new reference contig with the same
- name but a different length. Highly dangerous, only use if you know what you are doing.
- Default value: false. Possible values: {true, false}
+ U=Boolean If true, then permits mapping from a read contig to a new reference contig with the same
+ name but a different length. Highly dangerous, only use if you know what you are doing.
+ Default value: false. Possible values: {true, false}
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_ReplaceSamHeader.xml
--- a/picard_ReplaceSamHeader.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_ReplaceSamHeader.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,32 +6,32 @@
-
-
+
+
-
+
@@ -39,8 +39,8 @@
-
-
+
+
**Purpose**
@@ -50,7 +50,7 @@
@description@
- HEADER=File SAM file from which SAMFileHeader will be read. Required.
+ HEADER=File SAM file from which SAMFileHeader will be read. Required.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_RevertOriginalBaseQualitiesAndAddMateCigar.xml
--- a/picard_RevertOriginalBaseQualitiesAndAddMateCigar.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_RevertOriginalBaseQualitiesAndAddMateCigar.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,21 +6,20 @@
@@ -28,13 +27,13 @@
-
-
-
+
+
+
-
+
@@ -44,8 +43,8 @@
-
-
+
+
**Purpose**
@@ -57,11 +56,11 @@
@description@
RESTORE_ORIGINAL_QUALITIES=Boolean
- OQ=Boolean True to restore original qualities from the OQ field to the QUAL field if available.
- Default value: true. Possible values: {true, false}
-
- MAX_RECORDS_TO_EXAMINE=IntegerThe maximum number of records to examine to determine if we can exit early and not
- output, given that there are a no original base qualities (if we are to restore) and mate
+ OQ=Boolean True to restore original qualities from the OQ field to the QUAL field if available.
+ Default value: true. Possible values: {true, false}
+
+ MAX_RECORDS_TO_EXAMINE=IntegerThe maximum number of records to examine to determine if we can exit early and not
+ output, given that there are a no original base qualities (if we are to restore) and mate
cigars exist. Set to 0 to never skip the file. Default value: 10000.
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_RevertSam.xml
--- a/picard_RevertSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_RevertSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -6,31 +6,30 @@
@@ -50,13 +49,13 @@
-
-
-
+
+
+
-
+
@@ -73,8 +72,8 @@
-
-
+
+
**Purpose**
@@ -85,46 +84,46 @@
@description@
- SORT_ORDER=SortOrder
+ SORT_ORDER=SortOrder
SO=SortOrder The sort order to create the reverted output file with. Default value: queryname.
- Possible values: {unsorted, queryname, coordinate}
-
+ Possible values: {unsorted, queryname, coordinate}
+
RESTORE_ORIGINAL_QUALITIES=Boolean
- OQ=Boolean True to restore original qualities from the OQ field to the QUAL field if available.
- Default value: true. Possible values: {true, false}
-
+ OQ=Boolean True to restore original qualities from the OQ field to the QUAL field if available.
+ Default value: true. Possible values: {true, false}
+
REMOVE_DUPLICATE_INFORMATION=Boolean
- Remove duplicate read flags from all reads. Note that if this is true and
- REMOVE_ALIGNMENT_INFORMATION==false, the output may have the unusual but sometimes
- desirable trait of having unmapped reads that are marked as duplicates. Default value:
- true. Possible values: {true, false}
-
+ Remove duplicate read flags from all reads. Note that if this is true and
+ REMOVE_ALIGNMENT_INFORMATION==false, the output may have the unusual but sometimes
+ desirable trait of having unmapped reads that are marked as duplicates. Default value:
+ true. Possible values: {true, false}
+
REMOVE_ALIGNMENT_INFORMATION=Boolean
- Remove all alignment information from the file. Default value: true. TPossible values: {true, false}
-
- ATTRIBUTE_TO_CLEAR=String When removing alignment information, the set of optional tags to remove. This option may
- be specified 0 or more times.
-
- SANITIZE=Boolean WARNING: This option is potentially destructive. If enabled will discard reads in order
- to produce a consistent output BAM. Reads discarded include (but are not limited to)
- paired reads with missing mates, duplicated records, records with mismatches in length of
- bases and qualities. This option can only be enabled if the output sort order is
- queryname and will always cause sorting to occur. Possible values: {true, false}
-
- MAX_DISCARD_FRACTION=Double If SANITIZE=true and higher than MAX_DISCARD_FRACTION reads are discarded due to
- sanitization thenthe program will exit with an Exception instead of exiting cleanly.
- Output BAM will still be valid. Default value: 0.01.
-
+ Remove all alignment information from the file. Default value: true. TPossible values: {true, false}
+
+ ATTRIBUTE_TO_CLEAR=String When removing alignment information, the set of optional tags to remove. This option may
+ be specified 0 or more times.
+
+ SANITIZE=Boolean WARNING: This option is potentially destructive. If enabled will discard reads in order
+ to produce a consistent output BAM. Reads discarded include (but are not limited to)
+ paired reads with missing mates, duplicated records, records with mismatches in length of
+ bases and qualities. This option can only be enabled if the output sort order is
+ queryname and will always cause sorting to occur. Possible values: {true, false}
+
+ MAX_DISCARD_FRACTION=Double If SANITIZE=true and higher than MAX_DISCARD_FRACTION reads are discarded due to
+ sanitization thenthe program will exit with an Exception instead of exiting cleanly.
+ Output BAM will still be valid. Default value: 0.01.
+
SAMPLE_ALIAS=String
- ALIAS=String The sample alias to use in the reverted output file. This will override the existing
- sample alias in the file and is used only if all the read groups in the input file have
- the same sample alias Default value: null.
-
+ ALIAS=String The sample alias to use in the reverted output file. This will override the existing
+ sample alias in the file and is used only if all the read groups in the input file have
+ the same sample alias Default value: null.
+
LIBRARY_NAME=String
- LIB=String The library name to use in the reverted output file. This will override the existing
- sample alias in the file and is used only if all the read groups in the input file have
- the same sample alias Default value: null.
-
+ LIB=String The library name to use in the reverted output file. This will override the existing
+ sample alias in the file and is used only if all the read groups in the input file have
+ the same sample alias Default value: null.
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_SamToFastq.xml
--- a/picard_SamToFastq.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_SamToFastq.xml Tue Dec 06 10:04:41 2016 -0500
@@ -5,16 +5,17 @@
$report && ## This is necessary for output dataset detection (see output tags below)
-
+
@java_options@
-
+ @symlink_element_identifier@
+
picard
SamToFastq
-
- INPUT="${inputFile}"
-
+
+ INPUT='$inputFile.element_identifier'
+
#if str( $output_per_rg ) == "true":
OUTPUT_PER_RG=true
OUTPUT_DIR=.
@@ -25,34 +26,34 @@
#elif str( $output_per_rg ) == "false" and str( $interleave ) == "true":
FASTQ=INTERLEAVED.fastq
#end if
-
+
RE_REVERSE="${re_reverse}"
INTERLEAVE="${interleave}"
INCLUDE_NON_PF_READS="${include_non_pf_reads}"
CLIPPING_ATTRIBUTE="${clipping_attribute}"
CLIPPING_ACTION="${clipping_action}"
READ1_TRIM="${read1_trim}"
-
+
#if int($read1_max_bases_to_write) > -1:
READ1_MAX_BASES_TO_WRITE="${read1_max_bases_to_write}"
#end if
-
+
READ2_TRIM="${read2_trim}"
-
+
#if int($read2_max_bases_to_write) > -1:
READ2_MAX_BASES_TO_WRITE="${read2_max_bases_to_write}"
#end if
-
+
INCLUDE_NON_PRIMARY_ALIGNMENTS="${include_non_primary_alignments}"
-
-
+
+
VALIDATION_STRINGENCY="${validation_stringency}"
QUIET=true
VERBOSITY=ERROR
-
+
]]>
-
+
@@ -65,18 +66,18 @@
-
+
-
-
-
+
+
+
-
+
@@ -90,7 +91,7 @@
-
+
-
-
+
+
**Purpose**
@@ -120,71 +121,71 @@
@description@
FASTQ=File
- F=File Output fastq file (single-end fastq or, if paired, first end of the pair fastq).
+ F=File Output fastq file (single-end fastq or, if paired, first end of the pair fastq).
Required. Cannot be used in conjuction with option(s) OUTPUT_PER_RG (OPRG)
-
+
SECOND_END_FASTQ=File
- F2=File Output fastq file (if paired, second end of the pair fastq). Default value: null.
+ F2=File Output fastq file (if paired, second end of the pair fastq). Default value: null.
Cannot be used in conjuction with option(s) OUTPUT_PER_RG (OPRG)
-
+
UNPAIRED_FASTQ=File
- FU=File Output fastq file for unpaired reads; may only be provided in paired-fastq mode Default
+ FU=File Output fastq file for unpaired reads; may only be provided in paired-fastq mode Default
value: null. Cannot be used in conjuction with option(s) OUTPUT_PER_RG (OPRG)
-
+
OUTPUT_PER_RG=Boolean
- OPRG=Boolean Output a fastq file per read group (two fastq files per read group if the group is
+ OPRG=Boolean Output a fastq file per read group (two fastq files per read group if the group is
paired). Default value: false. Possible values: {true, false} Cannot be used in
conjuction with option(s) SECOND_END_FASTQ (F2) UNPAIRED_FASTQ (FU) FASTQ (F)
-
+
OUTPUT_DIR=File
- ODIR=File Directory in which to output the fastq file(s). Used only when OUTPUT_PER_RG is true.
- Default value: null.
-
+ ODIR=File Directory in which to output the fastq file(s). Used only when OUTPUT_PER_RG is true.
+ Default value: null.
+
RE_REVERSE=Boolean
- RC=Boolean Re-reverse bases and qualities of reads with negative strand flag set before writing them
- to fastq Default value: true. Possible values: {true, false}
-
+ RC=Boolean Re-reverse bases and qualities of reads with negative strand flag set before writing them
+ to fastq Default value: true. Possible values: {true, false}
+
INTERLEAVE=Boolean
- INTER=Boolean Will generate an interleaved fastq if paired, each line will have /1 or /2 to describe
- which end it came from Default value: false. Possible values: {true, false}
-
+ INTER=Boolean Will generate an interleaved fastq if paired, each line will have /1 or /2 to describe
+ which end it came from Default value: false. Possible values: {true, false}
+
INCLUDE_NON_PF_READS=Boolean
- NON_PF=Boolean Include non-PF reads from the SAM file into the output FASTQ files. PF means 'passes
- filtering'. Reads whose 'not passing quality controls' flag is set are non-PF reads.
- Default value: false. Possible values: {true, false}
-
+ NON_PF=Boolean Include non-PF reads from the SAM file into the output FASTQ files. PF means 'passes
+ filtering'. Reads whose 'not passing quality controls' flag is set are non-PF reads.
+ Default value: false. Possible values: {true, false}
+
CLIPPING_ATTRIBUTE=String
- CLIP_ATTR=String The attribute that stores the position at which the SAM record should be clipped Default
- value: null.
-
+ CLIP_ATTR=String The attribute that stores the position at which the SAM record should be clipped Default
+ value: null.
+
CLIPPING_ACTION=String
- CLIP_ACT=String The action that should be taken with clipped reads: 'X' means the reads and qualities
- should be trimmed at the clipped position; 'N' means the bases should be changed to Ns in
- the clipped region; and any integer means that the base qualities should be set to that
- value in the clipped region. Default value: null.
-
+ CLIP_ACT=String The action that should be taken with clipped reads: 'X' means the reads and qualities
+ should be trimmed at the clipped position; 'N' means the bases should be changed to Ns in
+ the clipped region; and any integer means that the base qualities should be set to that
+ value in the clipped region. Default value: null.
+
READ1_TRIM=Integer
- R1_TRIM=Integer The number of bases to trim from the beginning of read 1. Default value: 0.
-
+ R1_TRIM=Integer The number of bases to trim from the beginning of read 1. Default value: 0.
+
READ1_MAX_BASES_TO_WRITE=Integer
- R1_MAX_BASES=Integer The maximum number of bases to write from read 1 after trimming. If there are fewer than
- this many bases left after trimming, all will be written. If this value is null then all
- bases left after trimming will be written. Default value: null.
-
+ R1_MAX_BASES=Integer The maximum number of bases to write from read 1 after trimming. If there are fewer than
+ this many bases left after trimming, all will be written. If this value is null then all
+ bases left after trimming will be written. Default value: null.
+
READ2_TRIM=Integer
- R2_TRIM=Integer The number of bases to trim from the beginning of read 2. Default value: 0.
-
+ R2_TRIM=Integer The number of bases to trim from the beginning of read 2. Default value: 0.
+
READ2_MAX_BASES_TO_WRITE=Integer
- R2_MAX_BASES=Integer The maximum number of bases to write from read 2 after trimming. If there are fewer than
- this many bases left after trimming, all will be written. If this value is null then all
- bases left after trimming will be written. Default value: null.
-
+ R2_MAX_BASES=Integer The maximum number of bases to write from read 2 after trimming. If there are fewer than
+ this many bases left after trimming, all will be written. If this value is null then all
+ bases left after trimming will be written. Default value: null.
+
INCLUDE_NON_PRIMARY_ALIGNMENTS=Boolean
- If true, include non-primary alignments in the output. Support of non-primary alignments
- in SamToFastq is not comprehensive, so there may be exceptions if this is set to true and
+ If true, include non-primary alignments in the output. Support of non-primary alignments
+ in SamToFastq is not comprehensive, so there may be exceptions if this is set to true and
there are paired reads with non-primary alignments. Default value: false.
- Possible values: {true, false}
-
+ Possible values: {true, false}
+
@more_info@
diff -r 05087b27692a -r 7e6fd3d0f16e picard_SortSam.xml
--- a/picard_SortSam.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_SortSam.xml Tue Dec 06 10:04:41 2016 -0500
@@ -12,9 +12,10 @@
#set $output = $outFile
#end if
@java_options@
+ @symlink_element_identifier@
picard
SortSam
- INPUT="${inputFile}"
+ INPUT='$inputFile.element_identifier'
OUTPUT='${output}'
SORT_ORDER="${sort_order}"
QUIET=true
diff -r 05087b27692a -r 7e6fd3d0f16e picard_ValidateSamFile.xml
--- a/picard_ValidateSamFile.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_ValidateSamFile.xml Tue Dec 06 10:04:41 2016 -0500
@@ -16,7 +16,7 @@
&&
##set up input files
-
+ @symlink_element_identifier@
#set $reference_fasta_filename = "localref.fa"
#if str( $reference_source.reference_source_selector ) == "history":
@@ -30,7 +30,7 @@
picard
ValidateSamFile
- INPUT="${inputFile}"
+ INPUT='$inputFile.element_identifier'
OUTPUT="${outFile}"
MODE="${mode}"
diff -r 05087b27692a -r 7e6fd3d0f16e picard_macros.xml
--- a/picard_macros.xml Sun Nov 27 15:11:50 2016 -0500
+++ b/picard_macros.xml Tue Dec 06 10:04:41 2016 -0500
@@ -16,6 +16,10 @@
+
+