Mercurial > repos > devteam > sam_to_bam
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author | devteam |
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date | Tue, 21 Apr 2015 14:52:20 -0400 |
parents | c73bf16b45df |
children | 881e16ad05c6 |
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<tool id="sam_to_bam" name="SAM-to-BAM" version="2.0"> <description>convert SAM to BAM</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"></expand> <expand macro="version_command"></expand> <expand macro="stdio"></expand> <command> <![CDATA[ samtools sort -O bam -@ \${GALAXY_SLOTS:-1} -o sorted_input.bam -T temp "$input1" && #if $source.index_source == "history": ln -s $source.ref_file input.fa && samtools faidx input.fa && #else ln -s ${source.index.fields.path} input.fa && ln -s ${source.index.fields.path}.fai input.fa.fai && #end if samtools view -@ \${GALAXY_SLOTS:-1} -b -h -o $output1 -T input.fa sorted_input.bam ]]> </command> <inputs> <conditional name="source"> <param label="Choose the source for the reference genome" name="index_source" type="select"> <option value="cached">Use a built-in genome</option> <option value="history">Use a genome from the history</option> </param> <when value="cached"> <param format="sam" label="SAM file to convert" metadata_name="dbkey" name="input1" type="data"> <validator type="unspecified_build" /> <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="fasta_indexes" type="dataset_metadata_in_data_table" /> </param> <param label="Using reference genome" name="index" type="select"> <options from_data_table="fasta_indexes"> <filter column="1" key="dbkey" ref="input1" type="data_meta" /> <validator message="No reference genome is available for the build associated with the selected input dataset" type="no_options" /> </options> </param> </when> <when value="history"> <param format="sam" label="SAM file to convert" name="input1" type="data" /> <param format="fasta" label="Using reference file" metadata_name="dbkey" name="ref_file" type="data" /> </when> </conditional> </inputs> <outputs> <data format="bam" label="${tool.name} on ${on_string}: converted BAM" name="output1"> <actions> <conditional name="source.index_source"> <when value="cached"> <action name="dbkey" type="metadata"> <option name="source.input1" param_attribute="dbkey" type="from_param" /> </action> </when> <when value="history"> <action name="dbkey" type="metadata"> <option name="source.ref_file" param_attribute="dbkey" type="from_param" /> </action> </when> </conditional> </actions> </data> </outputs> <tests> <test> <param name="index_source" value="history" /> <param name="input1" ftype="sam" value="sam_to_bam_in1.sam" /> <param dbkey="equCab2" ftype="fasta" name="ref_file" value="chr_m.fasta" /> <output file="sam_to_bam_out1.bam" ftype="bam" name="output1" /> </test> </tests> <help> <![CDATA[ **What it does** Converts SAM dataset into its binary, BAM, representation using ``samtools sort`` and ``view`` commands:: samtools sort -O bam -o sorted_input.bam [INPUT SAM] samtools view -b -h -o -T [REFERENCE GENOME] [OUTPUT BAM] sorted_input.bam ]]> </help> <expand macro="citations"></expand> </tool>