Mercurial > repos > devteam > samtool_filter2
annotate samtool_filter2.xml @ 9:649a225999a5 draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 0d0561efe21c89202c258f4ecc5083cb84a137ba"
author | iuc |
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date | Sun, 24 Nov 2019 13:40:05 -0500 |
parents | a7add3e93cc5 |
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rev | line source |
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a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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1 <tool id="samtool_filter2" name="Filter SAM or BAM, output SAM or BAM" version="1.8+galaxy1"> |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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2 <description>files on FLAG MAPQ RG LN or by region</description> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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3 <requirements> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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4 <requirement type="package" version="1.8">samtools</requirement> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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diff
changeset
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5 </requirements> |
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a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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6 <macros> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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7 <xml name="flag_options"> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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8 <option value="0x0001">Read is paired</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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diff
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9 <option value="0x0002">Read is mapped in a proper pair</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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10 <option value="0x0004">The read is unmapped</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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11 <option value="0x0008">The mate is unmapped</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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changeset
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12 <option value="0x0010">Read is mapped to the reverse strand of the reference</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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13 <option value="0x0020">Mate is mapped to the reverse strand of the reference</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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14 <option value="0x0040">Read is the first in a pair</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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diff
changeset
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15 <option value="0x0080">Read is the second in a pair</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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16 <option value="0x0100">The alignment of this read is not primary</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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17 <option value="0x0200">The read fails platform/vendor quality checks</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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diff
changeset
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18 <option value="0x0400">The read is a PCR or optical duplicate</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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changeset
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19 <option value="0x0800">Supplementary alignment</option> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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20 </xml> |
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
iuc
parents:
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21 </macros> |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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22 <command detect_errors="exit_code"><![CDATA[ |
0 | 23 ##set up input files, regions requires input.bam and input.bai |
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b7aa423007bf
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit 94a7407227beb0139c49d92c7f922499dc7f0cd1
devteam
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24 #if $input1.is_of_type('bam') |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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25 #set $input = 'input.bam' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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26 ln -s '$input1' $input && |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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27 ln -s '$input1.metadata.bam_index' input.bai && |
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b7aa423007bf
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit 94a7407227beb0139c49d92c7f922499dc7f0cd1
devteam
parents:
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28 #elif $input1.is_of_type('sam') |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
parents:
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29 #set $input = 'input.sam' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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30 ln -s '$input1' $input && |
0 | 31 #end if |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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32 samtools view |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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33 $possibly_select_inverse '$output1' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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34 $header |
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94d5786febc4
planemo upload commit 4381f4022eca6f3c58ae1d01096795cd28c15062
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35 |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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36 #if $input1.is_of_type('sam') |
0 | 37 -S |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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38 #end if |
0 | 39 |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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40 #if str($outputtype) == 'bam' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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41 -b |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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42 #end if |
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94d5786febc4
planemo upload commit 4381f4022eca6f3c58ae1d01096795cd28c15062
devteam
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43 |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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44 #if str($mapq) |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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45 -q $mapq |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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46 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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47 #if $flag.filter == 'yes' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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48 #if str($flag.reqBits) != 'None' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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49 #set $reqs = str($flag.reqBits).split(',') |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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50 #set $reqFlag = 0 |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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51 #for $xn in $reqs: |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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52 #set $reqFlag += int($xn, 16) |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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53 #end for |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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54 -f $hex($reqFlag) |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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55 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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56 #if str($flag.skipBits) != 'None' |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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57 #set $skips = str($flag.skipBits).split(',') |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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58 #set $skipFlag = 0 |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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59 #for $xn in $skips: |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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60 #set $skipFlag += int(xn,16) |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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61 #end for |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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62 -F $hex($skipFlag) |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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63 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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64 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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65 #if str($read_group).strip() |
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planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit 94a7407227beb0139c49d92c7f922499dc7f0cd1
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66 -r '$read_group' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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67 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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68 #if str($library).strip() |
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planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit 94a7407227beb0139c49d92c7f922499dc7f0cd1
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69 -l '$library' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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70 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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71 #if $bed_file |
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b7aa423007bf
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit 94a7407227beb0139c49d92c7f922499dc7f0cd1
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72 -L '$bed_file' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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73 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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74 $input |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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75 #if str($regions).strip() and $input1.is_of_type('bam') |
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dc6ff68ea5e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit a97efd3ee05c6e27ff8294f66ea975445966d9c4
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76 #for region in str($regions).split(): |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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77 '$region' |
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dc6ff68ea5e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/samtool_filter2 commit a97efd3ee05c6e27ff8294f66ea975445966d9c4
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78 #end for |
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56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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79 #end if |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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80 ]]></command> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
iuc
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81 <inputs> |
56c31114ad4a
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82 <param name="input1" type="data" format="sam,bam" label="SAM or BAM file to filter" /> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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83 <param name="header" type="select" label="Header in output"> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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84 <option value="-h">Include header</option> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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85 <option value="">Exclude header</option> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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86 <option value="-H">Only the header</option> |
0 | 87 </param> |
7
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88 <param name="mapq" type="integer" min="0" value="" optional="true" label="Minimum MAPQ quality score" help="(-q)" /> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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89 <conditional name="flag"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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90 <param name="filter" type="select" label="Filter on bitwise flag"> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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91 <option value="no">no</option> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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92 <option value="yes">yes</option> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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93 </param> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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94 <when value="no"/> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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95 <when value="yes"> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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96 <param name="reqBits" type="select" multiple="true" display="checkboxes" label="Only output alignments with all of these flag bits set" help="(-f)"> |
8
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97 <expand macro="flag_options" /> |
7
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98 </param> |
56c31114ad4a
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99 <param name="skipBits" type="select" multiple="true" display="checkboxes" label="Skip alignments with any of these flag bits set" help="(-F)"> |
8
a7add3e93cc5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 2a605a22c4cb4b537644904d4ef74204ac9865e6
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100 <expand macro="flag_options" /> |
7
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101 </param> |
56c31114ad4a
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102 </when> |
56c31114ad4a
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103 </conditional> |
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104 <param name="library" type="text" value="" label="Select alignments from Library" |
56c31114ad4a
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105 help="(-l) Requires headers in the input SAM or BAM, otherwise no alignments will be output"/> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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106 <param name="read_group" type="text" value="" label="Select alignments from Read Group" |
56c31114ad4a
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107 help="(-r) Requires headers in the input SAM or BAM, otherwise no alignments will be output"/> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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108 <param name="bed_file" type="data" format="bed" optional="true" label="Output alignments overlapping the regions in the BED file" help="(-L)"/> |
56c31114ad4a
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109 <param name="possibly_select_inverse" type="boolean" truevalue="-U" falsevalue="-o" checked="false" label="Use inverse selection" help="Select the opposite of the listed chromosomes" /> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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110 <param name="regions" type="text" value="" label="Select regions (only used when the input is in BAM format)" |
56c31114ad4a
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111 help="region should be presented in one of the following formats: `chr1', `chr2:1,000' and `chr3:1000-2,000'"/> |
56c31114ad4a
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112 <param name="outputtype" type="select" label="Select the output format"> |
56c31114ad4a
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/samtool_filter2 commit 24d5defd0880276f60d3d2f6abed9a00fa57eb0e
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113 <option value="bam">BAM (-b)</option> |
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114 <option value="sam">SAM</option> |
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115 </param> |
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116 </inputs> |
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117 <outputs> |
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118 <data name="output1" format="sam" label="${tool.name} on ${on_string}: ${outputtype}"> |
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119 <change_format> |
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120 <when input="outputtype" value="bam" format="bam" /> |
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121 </change_format> |
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122 </data> |
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123 </outputs> |
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124 <tests> |
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125 <test> |
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126 <param name="input1" value="bam_to_sam_in2.sam" ftype="sam" /> |
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127 <param name="header" value=""/> |
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128 <param name="filter" value="yes"/> |
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129 <param name="reqBits" value="0x0080"/> |
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130 <param name="outputtype" value="sam"/> |
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131 <output name="output1"> |
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132 <assert_contents> |
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133 <has_text text="141" /> |
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134 <not_has_text text="77" /> |
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135 </assert_contents> |
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136 </output> |
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137 </test> |
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138 <test> |
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139 <param name="input1" value="bam_to_sam_in2.sam" ftype="sam" /> |
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140 <param name="header" value=""/> |
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141 <param name="filter" value="no"/> |
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142 <param name="read_group" value="rg1"/> |
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143 <param name="outputtype" value="sam"/> |
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144 <output name="output1"> |
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145 <assert_contents> |
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146 <has_text text="rg1" /> |
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147 <not_has_text text="rg2" /> |
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148 </assert_contents> |
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149 </output> |
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150 </test> |
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151 <test> |
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152 <param name="input1" value="bam_to_sam_in1.sam" ftype="sam" /> |
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153 <param name="header" value=""/> |
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154 <param name="filter" value="yes"/> |
56c31114ad4a
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155 <param name="skipBits" value="0x0008"/> |
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156 <param name="mapq" value="250"/> |
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157 <param name="outputtype" value="sam"/> |
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158 <output name="output1"> |
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159 <assert_contents> |
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160 <has_text text="both_reads_align_clip_marked" /> |
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161 <not_has_text text="both_reads_present_only_first_aligns" /> |
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162 </assert_contents> |
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163 </output> |
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164 </test> |
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165 </tests> |
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166 <help><![CDATA[ |
0 | 167 **What it does** |
168 | |
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169 This tool uses the samtools view command in SAMtools_ toolkit to filter a SAM or BAM file on the MAPQ (mapping quality), FLAG bits, Read Group, Library, or region. |
0 | 170 |
171 **Input** | |
172 | |
173 Input is either a SAM or BAM file. | |
174 | |
175 **Output** | |
176 | |
177 The output file will be SAM or BAM (depending on the chosen option), filtered by the selected options. | |
178 | |
179 **Options** | |
180 | |
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181 Filtering by read group or library requires headers in the input SAM or BAM file. |
0 | 182 |
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183 If regions are specified, only alignments overlapping the specified regions will be output. An alignment may be given multiple times if it is overlapping several regions. |
0 | 184 A region can be presented, for example, in the following format:: |
185 | |
186 chr2 (the whole chr2) | |
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187 chr2:1000000 (region starting from 1,000,000bp) |
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188 chr2:1,000,000-2,000,000 (region between 1,000,000 and 2,000,000bp including the end points). |
0 | 189 |
190 Note: The coordinate is 1-based. | |
191 | |
192 Multiple regions may be specified, separated by a space character:: | |
193 | |
194 chr2:1000000-2000000 chr2:1,000,000-2,000,000 chrX | |
195 | |
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196 .. _SAMtools: http://www.htslib.org/ |
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197 ]]></help> |
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198 <citations> |
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199 <citation type="doi">10.1093/bioinformatics/btp352</citation> |
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200 </citations> |
0 | 201 </tool> |