# HG changeset patch # User devteam # Date 1429643145 14400 # Node ID 8c34727900200419e42f812456b838031ee7adb3 # Parent c04bee391baad824c4843e7dc66fda0cc7580163 Uploaded diff -r c04bee391baa -r 8c3472790020 macros.xml --- a/macros.xml Wed Mar 18 16:43:50 2015 -0400 +++ b/macros.xml Tue Apr 21 15:05:45 2015 -0400 @@ -7,7 +7,37 @@ + + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + 10.1093/bioinformatics/btp352 + 10.1093/bioinformatics/btr076 + 10.1093/bioinformatics/btr509 + + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + + + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + + + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + + + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + @@ -18,4 +48,23 @@ + +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + + diff -r c04bee391baa -r 8c3472790020 samtools_bedcov.xml --- a/samtools_bedcov.xml Wed Mar 18 16:43:50 2015 -0400 +++ b/samtools_bedcov.xml Tue Apr 21 15:05:45 2015 -0400 @@ -1,11 +1,11 @@ - - on BAM files + + calculate read depth for a set of genomic intervals macros.xml + - **What it does** -This tool runs the ``samtools bedcov`` command in the SAMtools toolkit. +Calculates read depth for regions listed in a BED dataset using ``samtools bedcov`` command:: -Show read depth per BED region. + samtools bedcov [INPUT BED] [INPUT BAM1] ... [INPUT BAMn] > [OUTPUT]