# HG changeset patch # User iuc # Date 1494343138 14400 # Node ID 9129584416e24e4992bd27238ddfa73e8796cb17 # Parent bb40e4252392b88cfe055dd31ca19e3dc852b19b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_rmdup commit 411130b45dc30f7f24f41cdeec5e148c5d8faf40 diff -r bb40e4252392 -r 9129584416e2 macros.xml --- a/macros.xml Tue Dec 01 16:12:16 2015 -0500 +++ b/macros.xml Tue May 09 11:18:58 2017 -0400 @@ -1,16 +1,17 @@ - samtools + samtools + 1.3.1 @misc{SAM_def, title={Definition of SAM/BAM format}, - url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + url = {https://samtools.github.io/hts-specs/},} 10.1093/bioinformatics/btp352 10.1093/bioinformatics/btr076 @@ -41,7 +42,7 @@ - samtools --version | head -n 1 | awk '{ print $2 }' + &1 | grep Version]]> @@ -64,7 +65,5 @@ 5. Click **Save** The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. - - diff -r bb40e4252392 -r 9129584416e2 samtools_rmdup.xml --- a/samtools_rmdup.xml Tue Dec 01 16:12:16 2015 -0500 +++ b/samtools_rmdup.xml Tue May 09 11:18:58 2017 -0400 @@ -1,56 +1,56 @@ - - remove PCR duplicates - - macros.xml - - - samtools - - - - samtools rmdup - #if str( $bam_paired_end_type.bam_paired_end_type_selector ) == "PE" - ${bam_paired_end_type.force_se} - #else: - -s - #end if - "$input1" "$output1" - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + remove PCR duplicates + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + **What it does** -Remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends mapped to different chromosomes or orphan reads). This tool has the following parameters:: - - -s rmdup for SE reads - -S treat PE reads as SE in rmdup (force -s) - - - +Remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends mapped to different chromosomes or orphan reads). + + diff -r bb40e4252392 -r 9129584416e2 tool_dependencies.xml --- a/tool_dependencies.xml Tue Dec 01 16:12:16 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ - - - - - -