Mercurial > repos > devteam > vcfleftalign
diff vcfleftalign.xml @ 0:3e3d0dffeb1f draft
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author | devteam |
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date | Thu, 19 Mar 2015 14:41:53 -0400 |
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children | 8e02d116b0f9 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/vcfleftalign.xml Thu Mar 19 14:41:53 2015 -0400 @@ -0,0 +1,57 @@ +<tool id="vcfleftalign" name="VCFleftAlign:" version="0.0.3"> + <description>Left-align indels and complex variants in VCF dataset</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"></expand> + <expand macro="stdio"></expand> + <command> + #set $reference_fasta_filename = "localref.fa" + #if str( $reference_source.reference_source_selector ) == "history": + ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && + #else: + #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) + #end if + vcfleftalign -r "${reference_fasta_filename}" -w "${window_size}" "${input_vcf}" > "${out_file1}"</command> + <inputs> +<param name="input_vcf" type="data" format="vcf" label="VCF dataset to left-align" /> + <conditional name="reference_source"> + <param name="reference_source_selector" type="select" label="Choose the source for the reference genome"> + <option value="cached">Locally cached</option> + <option value="history">History</option> + </param> + <when value="cached"> + <param name="ref_file" type="select" label="Select reference genome"> + <options from_data_table="fasta_indexes"> + </options> + <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> + </param> + </when> + <when value="history"> <!-- FIX ME!!!! --> + <param name="ref_file" type="data" format="fasta" label="Using reference file" /> + </when> + </conditional> + <param name="window_size" type="integer" value="150" label="Use a window of this many bp when left-aligning" help="default = 150 bp" /> + </inputs> + <outputs> + <data format="vcf" name="out_file1" /> + </outputs> + <tests> + <test> + <param name="reference_source_selector" value="history" /> + <param name="input_vcf" value="vcflib-phix.vcf"/> + <param name="ref_file" value="vcflib-test-genome-phix.fa" /> + <param name="window_size" value="150" /> + <output name="out_file1" file="vcfleftalign-test1.vcf"/> + </test> + </tests> + <help> + +Left-aligns variants in VCF dataset. Window size is determined dynamically according to the entropy of the regions flanking the indel. These must have entropy > 1 bit/bp, or be shorter than ~5kb. + +---- + +Vcfleftalign @IS_PART_OF_VCFLIB@ +</help> + <expand macro="citations" /> +</tool>