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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/velvet commit ccce07eb8b612cbe09702214d3184b0085c055a8"
author iuc
date Sun, 24 Jan 2021 15:40:17 +0000
parents 8d09f8be269e
children
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1 <tool id="velveth" name="velveth" version="@WRAPPER_VERSION@.3">
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2 <description>Prepare a dataset for the Velvet velvetg Assembler</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="stdio"/>
1
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8 <version_command><![CDATA[
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9 velveth 2>&1 | grep "Version" | sed -e 's/Version //'
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10 ]]></version_command>
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11 <command><![CDATA[
2
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12 export OMP_NUM_THREADS="\${GALAXY_SLOTS:-1}" &&
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13 mkdir -p '${outfile.extra_files_path}' &&
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14 velveth
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15 '$outfile.extra_files_path'
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16 $hash_length
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17 #for $i in $inputs
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18 -fmtAuto
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19 ${i.input_type.read_type}
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20 #if str( $i.input_type.input_type_selector ) == "paireds":
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21 -separate '${i.input_type.input}' '${i.input_type.input2}'
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22 #else if str( $i.input_type.input_type_selector ) == "pairedi":
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23 -interleaved '${i.input_type.input}'
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24 #else:
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25 '${i.input_type.input}'
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26 #end if
2
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27 #end for
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28 $strand_specific
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29 ]]></command>
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30 <inputs>
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31 <param label="Hash Length" name="hash_length" type="integer" value="21" min="11" max="191" help="k-mer length in base pairs of the words being hashed."/>
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32 <param name="strand_specific" type="boolean" checked="false" truevalue="-strand_specific" falsevalue="" label="Use strand specific transcriptome sequencing" help="If you are using a strand specific transcriptome sequencing protocol, you may wish to use this option for better results."/>
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33 <repeat name="inputs" title="Input Files">
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34 <conditional name="input_type">
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35 <param name="input_type_selector" type="select" label="Choose the input type">
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36 <option value="single" selected="true">Single ended</option>
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37 <option value="paireds">separate paired end</option>
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38 <option value="pairedi">interleaved paired end</option>
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39 </param>
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40 <when value="single">
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41 <param label="read type" name="read_type" type="select">
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42 <option value="-short" selected="true">short reads</option>
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43 <option value="-short2">short2 reads</option>
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44 <option value="-short3">short3 reads</option>
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45 <option value="-short4">short4 reads</option>
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46 <option value="-long">long reads</option>
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47 </param>
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48 <param name="input" type="data" format="fasta,fastq,fasta.gz,fastq.gz" label="Dataset"/>
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49 </when>
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50 <when value="paireds">
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51 <param label="read type" name="read_type" type="select" >
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52 <option value="-shortPaired" selected="true">shortPaired reads</option>
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53 <option value="-shortPaired2">shortPaired2 reads</option>
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54 <option value="-shortPaired3">shortPaired3 reads</option>
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55 <option value="-shortPaired4">shortPaired4 reads</option>
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56 <option value="-longPaired">longPaired reads</option>
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57 </param>
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58 <param name="input" type="data" format="fasta,fastq,fasta.gz,fastq.gz" label="Dataset"/>
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59 <param name="input2" type="data" format="fasta,fastq,fasta.gz,fastq.gz" label="Dataset"/>
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60 </when>
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61 <when value="pairedi">
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62 <param label="read type" name="read_type" type="select">
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63 <option value="-shortPaired" selected="true">shortPaired reads</option>
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64 <option value="-shortPaired2">shortPaired2 reads</option>
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65 <option value="-shortPaired3">shortPaired3 reads</option>
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66 <option value="-shortPaired4">shortPaired4 reads</option>
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67 <option value="-longPaired">longPaired reads</option>
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68 </param>
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69 <param name="input" type="data" format="fasta,fastq,fasta.gz,fastq.gz" label="Dataset"/>
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70 </when>
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71 </conditional>
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72 </repeat>
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73 </inputs>
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74 <outputs>
2
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75 <data format="velvet" name="outfile" />
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76 </outputs>
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77 <tests>
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78 <test>
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79 <param name="hash_length" value="21" />
2
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80 <repeat name="inputs">
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81 <conditional name="input_type">
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82 <param name="input_type_selector" value="paireds" />
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83 <param name="read_type" value="-shortPaired" />
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84 <param name="input1" value="R1.fastq" ftype="fastqsanger" />
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85 <param name="input2" value="R2.fastq" ftype="fastqsanger" />
2
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86 </conditional>
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87 </repeat>
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88 <param name="strand_specific" value="" />
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89 <output name="outfile" file="velveth_paireds.out">
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90 <extra_files type="file" name='Sequences' value="velveth_paireds/Sequences" compare="diff" />
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91 <extra_files type="file" name='Roadmaps' value="velveth_paireds/Roadmaps" compare="diff" />
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92 </output>
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93 </test>
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94 <test>
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95 <param name="hash_length" value="21" />
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96 <repeat name="inputs">
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97 <conditional name="input_type">
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98 <param name="input_type_selector" value="single" />
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99 <param name="read_type" value="-short" />
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100 <param name="input" value="R1.fastq" ftype="fastqillumina" />
2
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101 </conditional>
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102 </repeat>
0
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103 <param name="strand_specific" value="" />
2
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104 <output name="outfile" file="velveth_single.out">
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105 <extra_files type="file" name='Sequences' value="velveth_single/Sequences" compare="diff" />
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106 <extra_files type="file" name='Roadmaps' value="velveth_single/Roadmaps" compare="diff" />
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107 </output>
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108 </test>
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109 <test>
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110 <param name="hash_length" value="21" />
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111 <repeat name="inputs">
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112 <conditional name="input_type">
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113 <param name="input_type_selector" value="single" />
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114 <param name="read_type" value="-short" />
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115 <param name="input" value="R1.fastq" ftype="fastq" />
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116 </conditional>
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117 </repeat>
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118 <repeat name="inputs">
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119 <param name="file_format" value="fastq" />
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120 <conditional name="input_type">
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121 <param name="input_type_selector" value="single" />
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122 <param name="read_type" value="-short2" />
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123 <param name="input" value="R2.fastq" ftype="fastq" />
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124 </conditional>
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125 </repeat>
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126 <param name="strand_specific" value="" />
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127 <output name="outfile" file="velveth_single.out">
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128 <extra_files type="file" name='Sequences' value="velveth_single2/Sequences" compare="diff" />
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129 <extra_files type="file" name='Roadmaps' value="velveth_single2/Roadmaps" compare="diff" />
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130 </output>
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131 </test>
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132 </tests>
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133 <help><![CDATA[
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134 **What it does**
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135
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136 Velvet_ is a de novo genomic assembler specially designed for short read sequencing technologies, such as Solexa or 454, developed by Daniel Zerbino and Ewan Birney at the European Bioinformatics Institute (EMBL-EBI), near Cambridge, in the United Kingdom.
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137
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138 Velvet currently takes in short read sequences, removes errors then produces high quality unique contigs. It then uses paired-end read and long read information, when available, to retrieve the repeated areas between contigs.
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139
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140 Read the Velvet `documentation`__ for details on using the Velvet Assembler.
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141
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142 .. _Velvet: http://www.ebi.ac.uk/~zerbino/velvet/
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143
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144 .. __: http://www.ebi.ac.uk/~zerbino/velvet/Manual.pdf
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145
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146 ------
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147
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148 **Velveth**
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149
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150 Velveth takes in a number of sequence files, produces a hashtable, then outputs two files in an output directory (creating it if necessary), Sequences and Roadmaps, which are necessary to velvetg.
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151
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152 ------
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153
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154 **Hash Length**
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155
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156 The hash length, also known as k-mer length, corresponds to the length, in base pairs, of the words being hashed.
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157
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158 The hash length is the length of the k-mers being entered in the hash table. Firstly, you must observe three technical constraints::
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159
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160 # it must be an odd number, to avoid palindromes. If you put in an even number, Velvet will just decrement it and proceed.
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161 # it must be below or equal to MAXKMERHASH length (cf. 2.3.3, by default 31bp), because it is stored on 64 bits
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162 # it must be strictly inferior to read length, otherwise you simply will not observe any overlaps between reads, for obvious reasons.
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163
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164 Now you still have quite a lot of possibilities. As is often the case, it's a trade- off between specificity and sensitivity. Longer kmers bring you more specificity (i.e. less spurious overlaps) but lowers coverage (cf. below). . . so there's a sweet spot to be found with time and experience.
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165 We like to think in terms of "k-mer coverage", i.e. how many times has a k-mer been seen among the reads. The relation between k-mer coverage Ck and standard (nucleotide-wise) coverage C is Ck = C # (L - k + 1)/L where k is your hash length, and L you read length.
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166 Experience shows that this kmer coverage should be above 10 to start getting decent results. If Ck is above 20, you might be "wasting" coverage. Experience also shows that empirical tests with different values for k are not that costly to run!
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167
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168 **Input Files**
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169
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170 Velvet works mainly with fasta and fastq formats. For paired-end reads, the assumption is that each read is next to its mate
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171 read. In other words, if the reads are indexed from 0, then reads 0 and 1 are paired, 2 and 3, 4 and 5, etc.
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172
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173 Supported file formats are::
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174
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175 fasta
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176 fastq
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177 fasta.gz
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178 fastq.gz
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179 eland
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180 gerald
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181
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182 Read categories are::
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183
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184 short (default)
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185 shortPaired
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186 short2 (same as short, but for a separate insert-size library)
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187 shortPaired2 (see above)
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188 long (for Sanger, 454 or even reference sequences)
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189 longPaired
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190 ]]></help>
0
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191 <expand macro="citation"/>
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192 </tool>