# HG changeset patch # User dnbenso # Date 1643343609 0 # Node ID 784cb0a6cfdb2456ddc2c43cde5141301294e737 # Parent 1808eaa9d699cdad4e0ccec9dc77c9ec13ca933d Uploaded diff -r 1808eaa9d699 -r 784cb0a6cfdb masurca.xml --- a/masurca.xml Tue Jan 25 03:45:18 2022 +0000 +++ b/masurca.xml Fri Jan 28 04:20:09 2022 +0000 @@ -10,14 +10,14 @@ cp $__tool_directory__/default-masurca-config config.txt && #if $nanopore_input.np_input == "Yes": #if $pacbio_input.pb_input == "Yes": - cat '$nanopore_input.nano' '$pacbio_input.pacbio' > long.fastq.gz && + cat '$nanopore_input.nano' '$pacbio_input.pacbio' > \$_GALAXY_JOB_TMP_DIR/long.fastq.gz && #else: - ln -s '$nanopore_input.nano' long.fastq.gz && + ln -s '$nanopore_input.nano' \$_GALAXY_JOB_TMP_DIR/long.fastq.gz && #end if - sed -i 's|#NANOPORE=INPUTREADLONG|NANOPORE=long.fastq.gz|' config.txt && + sed -i 's|#NANOPORE=INPUTREADLONG|NANOPORE='\$_GALAXY_JOB_TMP_DIR'/long.fastq.gz|' config.txt && #elif $pacbio_input.pb_input == "Yes": - ln -s '$pacbio_input.pacbio' long.fastq.gz && - sed -i 's|#PACBIO=INPUTREADLONG|PACBIO=long.fastq.gz|' config.txt && + ln -s '$pacbio_input.pacbio' \$_GALAXY_JOB_TMP_DIR/long.fastq.gz && + sed -i 's|#PACBIO=INPUTREADLONG|PACBIO='\$_GALAXY_JOB_TMP_DIR'/long.fastq.gz|' config.txt && #end if #if str( $illumina_input.input_type ) == "single" ln -s '$illumina_input.fastq_input1' ill_1.fastq.gz && @@ -119,6 +119,9 @@ flye == True + + flye == True + @@ -171,14 +174,14 @@ **Input data** -The following types of data are supported:: +The following types of data are supported: * Illumina paired end (or single end) reads -- MANDATORY. The mean and stdev parameters are the library insert average length and standard deviation. If the standard deviation is not known, set it to approximately 15% of the mean.If the second (reverse) read set is not available, do not specify it and just specify the forward reads. Files must be in fastq format and can be gzipped. * PacBio/MinION data are supported. Note that you have to have 50x + coverage in Illumina Paired End reads to use PacBio of Oxford Nanopore MinION data. Supply PacBio or MinION reads in a single fasta or fastq file (can be gzipped). **Parameters** -The following parameter is mandatory:: +The following parameter is mandatory: * jellyfish hash size, set this to about 10x the genome size.