Mercurial > repos > drosofff > blastn_to_scaffold
diff blastn_to_scaffold.xml @ 0:ad9a1c117ac6 draft default tip
planemo upload for repository https://bitbucket.org/drosofff/gedtools/
author | drosofff |
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date | Sun, 21 Jun 2015 14:41:10 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/blastn_to_scaffold.xml Sun Jun 21 14:41:10 2015 -0400 @@ -0,0 +1,47 @@ +<tool id="blastn2scaffold" name="blastn_to_scaffold" version="0.1.0"> +<description>Generate DNA scaffold from blastn alignment of Contigs</description> +<requirements> +</requirements> +<command interpreter="python"> + blastn_to_scaffold.py --sequences $sequences + --guideSequence $guideSequence + --blastn-tab $blastn_tab + --output $output +</command> +<inputs> +<param name="sequences" type="data" format="fasta" label="Select a fasta contigs file"/> +<param name="guideSequence" type="data" format="fasta" label="Select the fasta guide sequence for scaffolding"/> +<param name="blastn_tab" type="data" format="tabular" label="Select a blastn output from your history" help="must have 13 columns with column 13 containing the subject lenght, other columns are standard"/> + +</inputs> +<outputs> + <data format="fasta" name="output"/> +</outputs> + + +<tests> + <test> + <param name="sequences" value="contigs.fa" ftype="fasta"/> + <param name="blastn_tab" value="blastn.tab" ftype="tabular"/> + <param name="guideSequence" value="guideSequence.fa" ftype="tabular"/> + <output name="output" file="assembly.fa" ftype="fasta"/> + </test> +</tests> + + +<help> + + +**What it Does** +This tool start from DNA contigs that aligned to a subject DNA sequence through blastn. +The contigs must be provided in fasta format. The blastn output must be tabular, the 12 standard column plus column 13 with the length of the blastn subject. +The sequence used to blastn the contigs must be provided to serve as a guide to the final assembly +The final assembly is a DNA sequence. +Nucleotides of the guide sequence which were not covered by contigs are in small letters in the output assembly. + + +**Attribution** +This Galaxy tool was created by drosofff@gmail.com on 9/06/2015 +</help> + +</tool>