Mercurial > repos > drosofff > msp_oases
annotate README @ 2:b5df930313cc draft
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author | drosofff |
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date | Mon, 22 Jun 2015 10:39:20 -0400 |
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1 #Created 28/11/2014 |
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2 #Christophe Antoniewski, CNRS/University Pierre et Marie Curie, drosofff@gmail.com |
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3 # inspired from the oases wrapper developped by Konrad Paszkiewicz, Exeter Sequencing Service, University of Exeter |
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4 |
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5 Oases optimiser |
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6 |
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7 This Galaxy tool is intended to provide a crude but effective method of producing semi-optimised transcriptome assemblies. |
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8 It operates by performing a number of user defined Velvet assemblies upon which Oases is then run. |
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9 The results of all of these runs are then put through a final Oases step where they act as scaffolds for the main assembly. |
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10 Note that this wrapper is designed to focused on small RNA reads from small RNA sequencings and will run velvetg with kmers ranging from 11 to 35 |
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11 Prerequisites: |
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12 |
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13 1. Enclosed scripts |
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14 2. package_ged_velvet and package_ged_oases dependency packages |
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15 |
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16 Limitations: |
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17 |
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18 This is a crude optimisation step which DOES NOT try to evaluate the quality of each assembly or map to any reference cDNA. |