Mercurial > repos > drosofff > yac_clipper
comparison yac.xml @ 0:307cd074fa95 draft
planemo upload for repository https://bitbucket.org/drosofff/gedtools/
author | drosofff |
---|---|
date | Wed, 27 May 2015 17:40:52 -0400 |
parents | |
children | 8a8f62b4bf27 |
comparison
equal
deleted
inserted
replaced
-1:000000000000 | 0:307cd074fa95 |
---|---|
1 <tool id="yac" name="Clip adapter" version="1.3.3"> | |
2 <description /> | |
3 <command interpreter="python">yac.py --input $input | |
4 --output $output | |
5 --output_format "$out_format" | |
6 --adapter_to_clip $clip_source.clip_sequence | |
7 --min $min | |
8 --max $max | |
9 --Nmode $Nmode | |
10 </command> | |
11 <inputs> | |
12 <param format="fastq" label="Source file" name="input" type="data" /> | |
13 <param label="min size" name="min" size="4" type="integer" value="15" /> | |
14 <param label="max size" name="max" size="4" type="integer" value="36" /> | |
15 <param label="Select output format" name="out_format" type="select"> | |
16 <option selected="true" value="fasta">Fasta format</option> | |
17 <option value="fastq">Fastq format</option> | |
18 </param> | |
19 <param label="Accept reads containing N?" name="Nmode" type="select"> | |
20 <option selected="True" value="accept">accept</option> | |
21 <option value="reject">reject</option> | |
22 </param> | |
23 <conditional name="clip_source"> | |
24 <param help="Built-in adapters or User-provided" label="Source" name="clip_source_list" type="select"> | |
25 <option selected="True" value="prebuilt">Use a built-in adapter (select from the list below)</option> | |
26 <option value="user">Use custom sequence</option> | |
27 </param> | |
28 <when value="prebuilt"> | |
29 <param help="if your adapter is not listed, input your own sequence" label="Select Adapter to clip" name="clip_sequence" type="select"> | |
30 <option value="TCGTATGCCGTCTTCTGCTTG">Solexa TCGTATGCCGTCTTCTGCTTG</option> | |
31 <option value="ATCTCGTATGCCGTCTTCTGCTT">Illumina ATCTCGTATGCCGTCTTCTGCTT</option> | |
32 <option selected="True" value="TGGAATTCTCGGGTGCCAAG">Illumina TruSeq TGGAATTCTCGGGTGCCAAG</option> | |
33 <option value="CTGTAGGCACCATCAATCGT">IdT CTGTAGGCACCATCAATCGT</option> | |
34 </param> | |
35 </when> | |
36 <when value="user"> | |
37 <param label="Enter your Sequence" name="clip_sequence" size="35" type="text" value="GAATCC" /> | |
38 </when> | |
39 </conditional> | |
40 </inputs> | |
41 <outputs> | |
42 <data format="fasta" metadata="input" name="output" /> | |
43 <change_format> | |
44 <when format="fastq" input="out_format" value="fastq" /> | |
45 </change_format> | |
46 </outputs> | |
47 <tests> | |
48 <test> | |
49 <param ftype="fastqsanger" name="input" value="yac.fastq" /> | |
50 <param name="min" value="18" /> | |
51 <param name="max" value="29" /> | |
52 <param name="clip_source_list" value="prebuilt" /> | |
53 <param name="clip_sequence" value="ATCTCGTATGCCGTCTTCTGCTT" /> | |
54 <param name="Nmode" value="accept" /> | |
55 <output file="yac.out" name="output" /> | |
56 </test> | |
57 <test> | |
58 <param ftype="fastqsanger" name="input" value="yac.fastq" /> | |
59 <param name="min" value="18" /> | |
60 <param name="max" value="29" /> | |
61 <param name="clip_source_list" value="prebuilt" /> | |
62 <param name="clip_sequence" value="ATCTCGTATGCCGTCTTCTGCTT" /> | |
63 <param name="Nmode" value="accept" /> | |
64 <param name="out_format" value="fastq" /> | |
65 <output file="yac_fastq.out" name="output" /> | |
66 </test> | |
67 </tests> | |
68 <help> | |
69 This tool clips adapter sequences from a fastq file and outputs either a | |
70 fasta or fastq file of clipped reads with renumbered fasta/fastq headers. | |
71 | |
72 By defualt clipped sequences with unknown nucleotides are kept, but | |
73 can be discarded by setting "Accept reads containing N?" to reject. | |
74 | |
75 Min size and max size filter clipped reads on their size. | |
76 | |
77 Note that unclipped reads that satisfy the min and max size conditions are kept. | |
78 </help> | |
79 </tool> |