Mercurial > repos > earlhaminst > gstf_preparation
diff gstf_preparation.xml @ 8:92f3966d5bc3 draft
planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/gstf_preparation commit 88ba62ae8c3d9587a0015c72209242ad0c1df0c2
author | earlhaminst |
---|---|
date | Wed, 16 May 2018 20:03:57 -0400 |
parents | 9ef7661e8e9c |
children | e8e75a79de59 |
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--- a/gstf_preparation.xml Wed Apr 25 11:06:03 2018 -0400 +++ b/gstf_preparation.xml Wed May 16 20:03:57 2018 -0400 @@ -37,7 +37,7 @@ </param> </repeat> <param name="json" type="data" format="json" multiple="true" optional="true" label="Gene features in JSON format generated by 'Get features by Ensembl ID' tool" /> - <param name="fasta_inputs" type="data" format="fasta" multiple="true" label="Corresponding FASTA datasets" help="Each FASTA header line should start with a transcript id" /> + <param name="fasta_inputs" type="data" format="fasta" multiple="true" label="Corresponding CDS datasets in FASTA format" help="Each FASTA header line should start with a transcript id" /> <param name="longestCDS" type="boolean" checked="false" label="Keep only the longest CDS per gene" /> <param name="headers" type="boolean" checked="true" label="Change the header line of the FASTA sequences to the >TranscriptId_species format" help="As required by TreeBest, part of the GeneSeqToFamily workflow" /> <param name="regions" type="text" optional="true" label="Comma-separated list of region IDs (e.g. chromosomes or scaffolds) for which FASTA sequences should be filtered" help="Region IDs are in the `seqid` column for GFF3 and in the `seq_region_name` field in JSON. This is typically used to filter chromosomes with a non-standard genetic code, like mitochondria, to be analysed separately" /> @@ -111,7 +111,12 @@ This tool converts a set of GFF3 and/or JSON gene feature information datasets into SQLite format. -It also filters a CDS FASTA dataset to keep only the transcripts present in the gene feature information. Optionally it can also keep only the longest CDS per gene and/or change the header line of the FASTA sequences to the >TranscriptId_species format (as required by TreeBest, part of the GeneSeqToFamily workflow). +It also filters the CDS FASTA datasets to: + +- remove coding sequences whose length is not a multiple of 3 +- keep only the transcripts present in the gene feature information. + +Optionally it can also keep only the longest CDS per gene and/or change the header line of the FASTA sequences to the >TranscriptId_species format (as required by TreeBest, part of the GeneSeqToFamily workflow). Example GFF3 file::