<tool id="lotus2" name="LotuS2" version="@VERSION@" profile="20.01">
    <description>fast OTU processing pipeline</description>
    <macros>
        <token name="@VERSION@">2.05.1</token>
        <xml name="refDB_macro">
            <param argument="-refDB" type="select" label="Reference Database">
                <option value="SLV" selected="true">Silva LSU (23/28S) or SSU (16/18S) (SLV)</option>
                <option value="GG">Greengenes (GG)</option>
                <option value="UNITE">ITS focused on fungi (UNITE)</option>
                <option value="PR2">SSU focused on Protists (PR2)</option>
                <option value="beetax">Bee gut specific database and tax names (beetax)</option>
                <option value="HITdb">Human gut microbiota (HITdb)</option>
            </param>
        </xml>
    </macros>
    <requirements>
        <requirement type="package" version="@VERSION@">lotus2</requirement>
    </requirements>
    <version_command>lotus2 --version</version_command>
    <command detect_errors="exit_code"><![CDATA[
mkdir input
&&
#if $inputs.paired_or_single == 'single':
    #for i, f in enumerate($inputs.input):
        #set ext = $f.ext.replace('sanger', '')
        ln -s '$f' 'input/input${i}.${ext}' &&
    #end for
#elif $inputs.paired_or_single == 'paired':
    #for i, f in enumerate($inputs.left_input):
        #set ext = $f.ext.replace('sanger', '')
        ln -s '$f' 'input/input${i}.1.${ext}' &&
    #end for
    #for i, f in enumerate($inputs.right_input):
        #set ext = $f.ext.replace('sanger', '')
        ln -s '$f' 'input/input${i}.2.${ext}' &&
    #end for
#else:
    #for i, f in enumerate($inputs.pair_input):
        #set ext = $f.forward.ext.replace('sanger', '')
        ln -s '$f.forward' 'input/input${i}.1.${ext}' &&
        #set ext = $f.reverse.ext.replace('sanger', '')
        ln -s '$f.reverse' 'input/input${i}.2.${ext}' &&
    #end for
#end if

lotus2 -create_map mapping.txt -i input/ &&
cat mapping.txt &&

lotus2
-i input/
-o output
-tmpDir tmp_folder
-threads "\${GALAXY_SLOTS:-1}"
-map mapping.txt
-platform $platform
#if $barcode:
    -barcode '$barcode'
#end if
#if $forwardPrimer:
    -forwardPrimer '$forwardPrimer'
#end if
#if $reversePrimer:
    -reversePrimer '$reversePrimer'
#end if

-clustering $clu_args.clustering
-id $clu_args.id
#if $clu_args.derepMin:
    -derepMin '$clu_args.derepMin'
#end if
-deactivateChimeraCheck $clu_args.deactivateChimeraCheck
-chim_skew $clu_args.chim_skew
-readOverlap  $clu_args.readOverlap

-taxAligner $tax_args.aligner_cond.taxAligner
#if $tax_args.aligner_cond.taxAligner == '0':
    -rdp_thr $tax_args.aligner_cond.rdp_thr
#elif $tax_args.aligner_cond.taxAligner == '3':
    -utax_thr $tax_args.aligner_cond.utax_thr
#else:
    -refDB $tax_args.aligner_cond.refDB
#end if
-amplicon_type $tax_args.amplicon_type
-tax_group $tax_args.tax_group
-keepUnclassified $tax_args.keepUnclassified
-useBestBlastHitOnly $tax_args.useBestBlastHitOnly
-LCA_cover $tax_args.LCA_cover
-LCA_frac $tax_args.LCA_frac
-greengenesSpecies $tax_args.greengenesSpecies

; EXIT_VALUE=\$? ;

tar -cvzf output.tar.gz output/
&&
exit \$EXIT_VALUE
    ]]></command>

    <inputs>
        <conditional name="inputs">
            <param name="paired_or_single" type="select" label="Paired or Single-end data?">
                <option value="single" selected="true">Single-end</option>
                <option value="paired">Paired-end</option>
                <option value="paired_collection">Paired-end collection</option>
            </param>
            <when value="single">
                <param name="input" type="data" format="fastqsanger,fastqsanger.gz" multiple="true" label="Single-end reads" />
            </when>
            <when value="paired">
                <param name="left_input" type="data" format="fastqsanger,fastqsanger.gz" multiple="true" label="Left/Forward strand reads" />
                <param name="right_input" type="data" format="fastqsanger,fastqsanger.gz" multiple="true" label="Right/Reverse strand reads" />
            </when>
            <when value="paired_collection">
                <param name="pair_input" type="data_collection" collection_type="list:paired" format="fastqsanger,fastqsanger.gz" label="List of paired reads" />
            </when>
        </conditional>
        <param argument="-platform" type="select" label="Sequencing platform">
            <option value="miSeq" selected="true">miSeq</option>
            <option value="hiSeq">hiSeq</option>
            <option value="454">454</option>
            <option value="PacBio">PacBio</option>
        </param>
        <param argument="-barcode" type="data" format="fastqsanger" optional="true" label="Barcode (MID) sequences (optional)" help="FASTQ file with barcodes (in the processed mi/hiSeq format), if provided by the sequencer" />
        <param argument="-forwardPrimer" type="text" value="" label="Forward primer used to amplify DNA region" help="E.g. 16S primer fwd" />
        <param argument="-reversePrimer" type="text" value="" label="Reverse primer used to amplify DNA region" help="E.g. 16S primer rev" />
        <section name="clu_args" title="Clustering Options">
            <param argument="-clustering" type="select" label="Clustering algorithm">
                <option value="1">UPARSE</option>
                <option value="2">swarm</option>
                <option value="3">cd-hit</option>
                <option value="6">unoise3</option>
                <option value="7" selected="true">dada2</option>
            </param>
            <param argument="-id" type="float" min="0" max="1" value="0.97" label="Clustering threshold for OTUs" />
            <param argument="-derepMin" type="text" value="" label="Minimum size of dereplicated raw reads" help="E.g. 4:1,4:2,3:3 . See http://lotus2.earlham.ac.uk/images/Derep_options.pdf for how to specify this parameter" />
            <param argument="-deactivateChimeraCheck" type="select" label="Chimera check">
                <option value="0" selected="true">OTU chimera checks</option>
                <option value="1">No chimera check at all</option>
                <option value="2">Deactivate deNovo chimera check</option>
                <option value="3">Deactivate ref based chimera check</option>
            </param>
            <param argument="-chim_skew" type="integer" min="0" value="2" label="Skew in chimeric fragment abundance" />
            <param argument="-readOverlap" type="integer" min="0" value="300" label="Maximum number of basepairs that two reads are overlapping" />
        </section>
        <section name="tax_args" title="Taxonomy Options">
            <conditional name="aligner_cond">
                <param argument="-taxAligner" type="select" label="Taxonomy aligner">
                    <option value="0" selected="true">Deactivated (just use RDP)</option>
                    <option value="1">Blast</option>
                    <option value="2">Use LAMBDA to search against a 16S reference database for taxonomic profiling of OTUs</option>
                    <option value="3">Use UTAX with custom databases</option>
                    <option value="4">Use VSEARCH to align OTUs to custom databases</option>
                </param>
                <when value="0">
                    <param argument="-rdp_thr" type="float" min="0" max="1" value="0.8" label="Confidence threshold for RDP"/>
                </when>
                <when value="1">
                    <expand macro="refDB_macro" />
                </when>
                <when value="2">
                    <expand macro="refDB_macro" />
                </when>
                <when value="3">
                    <param argument="-utax_thr" type="float" min="0" max="1" value="0.8" label="Confidence threshold for UTAX"/>
                </when>
                <when value="4">
                    <expand macro="refDB_macro" />
                </when>
            </conditional>
            <param argument="-amplicon_type" type="select" label="Amplicon type">
                <option value="LSU">LSU Large subunit (23S/28S)</option>
                <option value="SSU" selected="true">SSU small subunit (16S/18S)</option>
                <option value="ITS">ITS internal transcribed spacer</option>
                <option value="ITS1">ITS1</option>
                <option value="ITS2">ITS2</option>
            </param>
            <param argument="-tax_group" type="select" label="Tax group">
                <option value="bacteria" selected="true">bacterial 16S rDNA annnotation</option>
                <option value="fungi">fungal 18S/23S/ITS annotation</option>
            </param>
            <param argument="-keepUnclassified" type="boolean" truevalue="1" falsevalue="0" checked="true" label="Keep unclassified OTUs" help="Includes unclassified OTUs (i.e. no match in RDP/Blast database) in OTU and taxa abundance matrix calculations" />
            <param argument="-useBestBlastHitOnly" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Use best blast hit only" help="If selected, do not use LCA (lowest common ancestor) to determine most likely taxonomic level (not recommended)" />
            <param argument="-LCA_cover" type="float" min="0" max="1" value="0.9" label="Minimum horizontal coverage of an OTU sequence against ref DB"/>
            <param argument="-LCA_frac" type="float" min="0" max="1" value="0.9" label="Minimum fraction of reads with identical taxonomy"/>
            <param argument="-greengenesSpecies" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Create greengenes output labels instead of OTU" />
        </section>
    </inputs>

    <outputs>
        <data name="otu" format="tabular" label="${tool.name} on ${on_string}: OTU abundance matrix" from_work_dir="output/OTU.txt" />
        <data name="otu_biom" format="biom" label="${tool.name} on ${on_string}: biom-formatted OTU abundance matrix" from_work_dir="output/OTU.biom" />
        <data name="otu_fna" format="fasta" label="${tool.name} on ${on_string}: FASTA-formatted extended OTU seed sequences" from_work_dir="output/OTU.fna" />
        <data name="OTUphylo_nwk" format="newick" label="${tool.name} on ${on_string}: Newick-formatted phylogenetic tree between sequences" from_work_dir="output/OTUphylo.nwk" />
        <data name="hiera_blast" format="tabular" label="${tool.name} on ${on_string}: OTU taxonomy assignments based on Blastn" from_work_dir="output/hiera_BLAST.txt" />
        <data name="hiera_rdp" format="tabular" label="${tool.name} on ${on_string}: OTU taxonomy assignments based on RDP classifier" from_work_dir="output/hiera_RDP.txt" />
        <data name="primary" format="tar" label="${tool.name} on ${on_string}: All output files" from_work_dir="output.tar.gz" />
    </outputs>

    <tests>
        <test>
            <param name="paired_or_single" value="single"/>
            <param name="input" value="Anh_sample1.fastq.gz,Anh_sample2.fastq.gz" ftype="fastqsanger.gz"/>
            <param name="platform" value="454" />
            <param name="clustering" value="3" />
            <output name="otu" file="OTU.txt" compare="sim_size" />
            <output name="otu_fna" file="OTU.fna" compare="sim_size" />
            <output name="hiera_rdp" file="hiera_RDP.txt" compare="sim_size" />
        </test>
    </tests>

    <help><![CDATA[
If you have separate FASTA and quality files, these can be combined in a FASTQ file using the "Combine FASTA and QUAL into FASTQ" tool.

Documentation can be found at `<http://lotus2.earlham.ac.uk/>`_.
    ]]></help>
    <citations>
        <citation type="doi">10.1186/s40168-021-01012-1</citation>
    </citations>
</tool>