Mercurial > repos > ebi-gxa > monocle3_create
diff monocle3-create.xml @ 0:75340a5e5fb0 draft
"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit 815e36252846236124957052433572f1c1247114"
author | ebi-gxa |
---|---|
date | Mon, 16 Sep 2019 09:52:55 -0400 |
parents | |
children | a34f243f55a4 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/monocle3-create.xml Mon Sep 16 09:52:55 2019 -0400 @@ -0,0 +1,82 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="monocle3_create" name="Monocle3 create" version="@TOOL_VERSION@+galaxy0"> + <description>a Monocle3 object from input data</description> + <macros> + <import>monocle3-macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +ln -s '${expression_matrix}' expression_matrix.'${expression_matrix_format}' && +#if $cell_metadata +ln -s '${cell_metadata}' cell_metadata.'${cell_metadata_format}' && +#end if +#if $gene_annotation +ln -s '${gene_annotation}' gene_annotation.'${gene_annotation_format}' && +#end if +LANG=en_US.UTF-8 monocle3 create + --expression-matrix expression_matrix.'${expression_matrix_format}' +#if $cell_metadata + --cell-metadata cell_metadata.'${cell_metadata_format}' +#end if +#if $gene_annotation + --gene-annotation gene_annotation.'${gene_annotation_format}' +#end if + @OUTPUT_OPTS@ + @VERBOSE@ +]]></command> + + <inputs> + <param name="expression_matrix" argument="--expression-matrix" type="data" format="tsv,csv,rdata" label="Expression matrix, genes as rows, cells as columns. Required input. Provide as TSV, CSV or RDS."/> + <param name="expression_matrix_format" type="select" label="Format of expression matrix"> + <option value="rds" selected="true">RDS</option> + <option value="tsv">TSV</option> + <option value="csv">CSV</option> + </param> + <param name="cell_metadata" argument="--cell-metadata" optional="true" type="data" format="tsv,csv,rdata" label="Per-cell annotation, optional. Row names must match the column names of the expression matrix. Provide as TSV, CSV or RDS."/> + <param name="cell_metadata_format" type="select" label="Format of cell metadata"> + <option value="rds" selected="true">RDS</option> + <option value="tsv">TSV</option> + <option value="csv">CSV</option> + </param> + <param name="gene_annotation" argument="--gene-annotation" optional="true" type="data" format="tsv,csv,rdata" label="Per-gene annotation, optional. Row names must match the row names of the expression matrix. Provide as TSV, CSV or RDS."/> + <param name="gene_annotation_format" type="select" label="Format of gene annotation"> + <option value="rds" selected="true">RDS</option> + <option value="tsv">TSV</option> + <option value="csv">CSV</option> + </param> + <expand macro="output_object_params"/> + <expand macro="verbose_flag"/> + </inputs> + + <outputs> + <data name="output_rds" format="rdata" from_work_dir="output.RDS" label="${tool.name} on ${on_string}: ${output_object_format}"/> + </outputs> + + <tests> + <test> + <param name="expression_matrix" value="expression.RDS"/> + <param name="cell_metadata" value="cells.RDS"/> + <param name="gene_annotation" value="genes.RDS"/> + <param name="output_object_format" value="cds3"/> + <output name="output_rds" file="output.rds" ftype="rdata" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +=========================================================================== +Create Monocle3 object from input expression/metadata (`new_cell_data_set`) +=========================================================================== + +Create a Monocle3 cds3 object from input expression data and optional metadata. +The expression matrix, with genes as rows and cells as columns, is the only mandatory +input. The cell metadata (with row names matching the column names of the expression +matrix) and gene annotation (with row names matching the row names of the expression +matrix) are optional. These files can be provided as RDS objects, or CSV/TSV. Select the +format of your input files in the corresponding drop-downs. + +@HELP@ + +@VERSION_HISTORY@ +]]></help> + <expand macro="citations"/> +</tool>