Mercurial > repos > ebi-gxa > monocle3_preprocess
diff monocle3-preprocess.xml @ 0:384b9bcf2807 draft
"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit 815e36252846236124957052433572f1c1247114"
author | ebi-gxa |
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date | Mon, 16 Sep 2019 09:50:29 -0400 |
parents | |
children | 0869b0264af1 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/monocle3-preprocess.xml Mon Sep 16 09:50:29 2019 -0400 @@ -0,0 +1,84 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="monocle3_preprocess" name="Monocle3 preprocess" version="@TOOL_VERSION@+galaxy0"> + <description>a Monocle3 object to an initially dimensionally reduced space</description> + <macros> + <import>monocle3-macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +LANG=en_US.UTF-8 monocle3 preprocess + --method '${method}' +#if $num_dim + --num-dim '${num_dim}' +#end if + --norm-method '${norm_method}' +#if $use_genes + --use-genes '${use_genes}' +#end if +#if $residual_model_formula_str + --residual-model-formula-str '~${residual_model_formula_str}' +#end if +#if $pseudo_count + --pseudo-count '${pseudo_count}' +#end if +#if $no_scaling + --no-scaling +#end if + + @INPUT_OPTS@ + @OUTPUT_OPTS@ + + @VERBOSE@ +]]></command> + + <inputs> + <expand macro="input_object_params"/> + <expand macro="output_object_params"/> + <param name="method" argument="--method" type="select" label="Initial dimensionality reduction to perform."> + <option value="PCA" selected="true">PCA</option> + <option value="LSI">LSI</option> + </param> + <param name="num_dim" argument="--num-dim" optional="true" type="integer" value="50" label="The dimensionality of the reduced space."/> + <param name="norm_method" argument="--norm-method" type="select" label="Determines how to transform expression values prior to reducing dimensionality."> + <option value="log" selected="true">Size factor correction and log transformation</option> + <option value="size_only">Size factor correction only</option> + </param> + <param name="use_genes" argument="--use-genes" type="text" optional="true" label="Manually subset the gene pool to these genes for dimensionality reduction."/> + <param name="residual_model_formula_str" argument="--residual-model-formula-str" type="text" optional="true" label="A string model formula specifying effects to subtract from the data (excluding the leading ~)."/> + <param name="pseudo_count" argument="--pseudo-count" optional="true" type="float" value="1" label="Amount to increase expression values before dimensionality reduction."/> + <param name="no_scaling" argument="--no-scaling" type="boolean" checked="false" label="When this option is NOT set, scale each gene before running trajectory reconstruction."/> + <expand macro="verbose_flag"/> + </inputs> + + <outputs> + <data name="output_rds" format="rdata" from_work_dir="output.RDS" label="${tool.name} on ${on_string}: ${output_object_format}"/> + </outputs> + + <tests> + <test> + <param name="input_object_file" value="input.RDS"/> + <param name="input_object_format" value="cds3"/> + <param name="method" value="PCA"/> + <param name="num_dim" value="50"/> + <param name="norm_method" value="log"/> + <param name="pseudo_count" value="1"/> + <param name="output_object_format" value="cds3"/> + <output name="output_rds" file="output.rds" ftype="rdata" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +==================================================================================== +Preprocess Monocle3 object up to initial dimensionality reduction (`preprocess_cds`) +==================================================================================== + +Preprocess a Monocle3 object with size factor scaling of the count data, optionally +log transforming, and computing an initial dimensionality reduction. This space is +used as a starting point for a number of subsequent analyses. + +@HELP@ + +@VERSION_HISTORY@ +]]></help> + <expand macro="citations"/> +</tool>