Mercurial > repos > ebi-gxa > scanpy_filter_genes
comparison scanpy-filter-genes.xml @ 3:77fbe74247c2 draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit ccd9b839849600b1aa3b6cf54f667575a5f2da9d
author | ebi-gxa |
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date | Fri, 25 Oct 2019 08:21:35 -0400 |
parents | a797a810d7e9 |
children | fa6c08064fc1 |
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2:a797a810d7e9 | 3:77fbe74247c2 |
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7 <expand macro="requirements"/> | 7 <expand macro="requirements"/> |
8 <command detect_errors="exit_code"><![CDATA[ | 8 <command detect_errors="exit_code"><![CDATA[ |
9 ln -s '${input_obj_file}' input.h5 && | 9 ln -s '${input_obj_file}' input.h5 && |
10 PYTHONIOENCODING=utf-8 scanpy-filter-genes | 10 PYTHONIOENCODING=utf-8 scanpy-filter-genes |
11 #if $parameters | 11 #if $parameters |
12 #set pars = ' '.join(['--param {name} {min} {max}'.format(**$p) for $p in $parameters]) | 12 #set pars = ' '.join(['--param g:{name} {min} {max}'.format(**$p) for $p in $parameters]) |
13 ${pars} | 13 ${pars} |
14 #end if | 14 #end if |
15 #if $categories | 15 #if $categories |
16 #set cats = ' '.join(['--category {name} {values}'.format(**$c) for $c in $categories]) | 16 #set cats = ' '.join(['--category g:{name} {values}'.format(**$c) for $c in $categories]) |
17 ${cats} | 17 ${cats} |
18 #end if | 18 #end if |
19 #if $subsets | 19 #if $subsets |
20 #set subs = ' '.join(['--subsets {name} {subset}'.format(**$s) for $s in $subsets]) | 20 #set subs = ' '.join(['--subset g:{name} {subset}'.format(**$s) for $s in $subsets]) |
21 ${subs} | 21 ${subs} |
22 #end if | 22 #end if |
23 @INPUT_OPTS@ | 23 @INPUT_OPTS@ |
24 @OUTPUT_OPTS@ | 24 @OUTPUT_OPTS@ |
25 ]]></command> | 25 ]]></command> |
26 | 26 |
27 <inputs> | 27 <inputs> |
28 <expand macro="input_object_params"/> | 28 <expand macro="input_object_params"/> |
29 <expand macro="output_object_params"/> | 29 <expand macro="output_object_params"/> |
30 | 30 |
31 <repeat name="parameters" title="Parameters used to filter cells" min="1"> | 31 <repeat name="parameters" title="Parameters used to filter genes" min="1"> |
32 <param name="name" type="text" value="n_cells" label="Name of parameter to filter on" help="for example n_genes or n_counts"> | 32 <param name="name" type="text" value="n_cells" label="Name of parameter to filter on" help="for example n_genes or n_counts"> |
33 <option value="n_cells">n_cells</option> | 33 <option value="n_cells">n_cells</option> |
34 <option value="gene:n_counts">n_counts</option> | 34 <option value="n_counts">n_counts</option> |
35 </param> | 35 </param> |
36 <param name="min" type="float" min="0" value="0" label="Min value"/> | 36 <param name="min" type="float" min="0" value="0" label="Min value"/> |
37 <param name="max" type="float" min="0" value="1e9" label="Max value"/> | 37 <param name="max" type="float" min="0" value="1e9" label="Max value"/> |
38 </repeat> | 38 </repeat> |
39 | 39 |
40 <repeat name="categories" title="Categories used to filter genes" min="0"> | 40 <repeat name="categories" title="Categories used to filter genes" min="0"> |
41 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/> | 41 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/> |
42 <param name="values" type="text" value="" label="Comma-separated values"/> | 42 <param name="values" type="text" value="" label="Comma-separated values to keep"/> |
43 </repeat> | 43 </repeat> |
44 | 44 |
45 <repeat name="subsets" title="Subsets used to filter genes" min="0"> | 45 <repeat name="subsets" title="Subsets used to filter genes" min="0"> |
46 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/> | 46 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/> |
47 <param name="subset" type="data" format="tabular" label="List of values"/> | 47 <param name="subset" type="data" format="tabular" label="List of values to keep" help="A one-column headerless text file is required"/> |
48 </repeat> | 48 </repeat> |
49 <expand macro="export_mtx_params"/> | 49 <expand macro="export_mtx_params"/> |
50 </inputs> | 50 </inputs> |
51 | 51 |
52 <outputs> | 52 <outputs> |
63 <param name="name" value="n_cells"/> | 63 <param name="name" value="n_cells"/> |
64 <param name="min" value="3"/> | 64 <param name="min" value="3"/> |
65 <param name="max" value="1e9"/> | 65 <param name="max" value="1e9"/> |
66 </repeat> | 66 </repeat> |
67 <repeat name="parameters"> | 67 <repeat name="parameters"> |
68 <param name="name" value="gene:n_counts"/> | 68 <param name="name" value="n_counts"/> |
69 <param name="min" value="0"/> | 69 <param name="min" value="0"/> |
70 <param name="max" value="1e9"/> | 70 <param name="max" value="1e9"/> |
71 </repeat> | 71 </repeat> |
72 <output name="output_h5" file="filter_genes.h5" ftype="h5" compare="sim_size"/> | 72 <output name="output_h5" file="filter_genes.h5" ftype="h5" compare="sim_size"/> |
73 </test> | 73 </test> |
74 </tests> | 74 </tests> |
75 | 75 |
76 <help><![CDATA[ | 76 <help><![CDATA[ |
77 =================================================================== | 77 ===================================================================== |
78 Filter genes based on number of cells or counts (`pp.filter_genes`) | 78 Filter genes based on arbitrary attributes (`scanpy.pp.filter_genes`) |
79 =================================================================== | 79 ===================================================================== |
80 | 80 |
81 Keep genes that have at least `min_counts` counts or are expressed in at | 81 Keep genes that have at least `min_counts` counts or are expressed in at |
82 least `min_cells` cells or have at most `max_counts` counts or are expressed | 82 least `min_cells` cells or have at most `max_counts` counts or are expressed |
83 in at most `max_cells` cells. | 83 in at most `max_cells` cells. Other gene attributes can be used for filtering |
84 | 84 too if available. |
85 Only provide one of the optional parameters `min_counts`, `min_cells`, | |
86 `max_counts`, `max_cells` per call. | |
87 | 85 |
88 | 86 |
89 @HELP@ | 87 @HELP@ |
90 | 88 |
91 @VERSION_HISTORY@ | 89 @VERSION_HISTORY@ |