Mercurial > repos > ebi-gxa > scanpy_find_markers
diff scanpy-find-markers.xml @ 0:c608fd80ec15 draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
author | ebi-gxa |
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date | Wed, 03 Apr 2019 11:08:46 -0400 |
parents | |
children | 71668dd2d47b |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/scanpy-find-markers.xml Wed Apr 03 11:08:46 2019 -0400 @@ -0,0 +1,94 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="scanpy_find_markers" name="Scanpy FindMarkers" version="@TOOL_VERSION@+galaxy1"> + <description>to find differentially expressed genes between groups</description> + <macros> + <import>scanpy_macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +ln -s '${input_obj_file}' input.h5 && +PYTHONIOENCODING=utf-8 scanpy-find-markers.py + -i input.h5 + -f '${input_format}' + -o output.h5 + -F '${output_format}' + -n '${n_genes}' + #if $output_markers + --output-text-file output.csv + #end if + #if $settings.default == "false" + -g '${settings.groupby}' + --reference '${settings.reference}' + --method '${settings.method}' + #if $settings.use_raw == "false" + --no-raw + #end if + #if $settings.rankby_abs + --rankby_abs + #end if + #if $settings.groups + --groups '${settings.groups}' + #end if +#end if +]]></command> + + <inputs> + <expand macro="input_object_params"/> + <expand macro="output_object_params"/> + <param name="n_genes" argument="--n-genes" type="integer" value="50" label="Number of top genes to show per group/cluster"/> + <param name="output_markers" type="boolean" checked="true" label="Output markers table in csv format"/> + <conditional name="settings"> + <param name="default" type="boolean" checked="true" label="Use programme defaults"/> + <when value="true"/> + <when value="false"> + <param name="groupby" argument="--groupby" type="text" value="louvain" label="The sample grouping/clustering to use."/> + <param name="use_raw" type="boolean" checked="true" label="Use raw attribute if present"/> + <param name="reference" argument="--reference" type="text" value="rest" label="If 'rest', compare to the union of the rest of the group/cluster. If a group identifier, compare to that group"/> + <param name="method" argument="--method" type="select" label="Method for testing differentially expressed genes"> + <option value="t-test_overestim_var" selected="true">t-test with over-estimated variance</option> + <option value="t-test">t-test</option> + <option value="wilcoxon">wilcoxon test, currently broken don't use</option> + <option value="logreg">logistic regression</option> + </param> + <param name="rankby_abs" argument="--rankby_abs" type="boolean" checked="false" label="Rank by absolute value of the scores instead of the scores"/> + <param name="groups" argument="--groups" optional="true" type="text" label="Subset of groups/clusters to which comparisons shell be restricted."/> + </when> + </conditional> + </inputs> + + <outputs> + <data name="output_h5" format="h5" from_work_dir="output.h5" label="${tool.name} on ${on_string}: Marker object"/> + <data name="output_csv" format="csv" from_work_dir="output.csv" label="${tool.name} on ${on_string}: Marker table"> + <filter>output_markers</filter> + </data> + </outputs> + + <tests> + <test> + <param name="input_obj_file" value="find_cluster.h5"/> + <param name="input_format" value="anndata"/> + <param name="output_format" value="anndata"/> + <param name="output_csv" value="true"/> + <param name="n_genes" value="50"/> + <param name="output_markers" value="false"/> + <param name="default" value="false"/> + <param name="groupby" value="louvain"/> + <param name="method" value="t-test_overestim_var"/> + <param name="rankby_abs" value="false"/> + <output name="output_h5" file="find_markers.h5" ftype="h5" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +============================================================= +Rank genes for characterizing groups (`tl.rank_genes_groups`) +============================================================= + +Rank genes for characterizing groups. + +@HELP@ + +@VERSION_HISTORY@ +]]></help> + <expand macro="citations"/> +</tool>