view scanpy-plot-embedding.xml @ 1:ddb20964072e draft

planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit ccd9b839849600b1aa3b6cf54f667575a5f2da9d
author ebi-gxa
date Fri, 25 Oct 2019 08:23:35 -0400
parents c63bc0b71865
children b2410da4e381
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<?xml version="1.0" encoding="utf-8"?>
<tool id="scanpy_plot_embed" name="Scanpy PlotEmbed" version="@TOOL_VERSION@+galaxy0">
  <description>visualise cell embeddings</description>
  <macros>
    <import>scanpy_macros2.xml</import>
  </macros>
  <expand macro="requirements"/>
  <command detect_errors="exit_code"><![CDATA[
ln -s '${input_obj_file}' input.h5 &&
PYTHONIOENCODING=utf-8 scanpy-cli plot embed
    @INPUT_OPTS@
    --basis '${basis}'
    --color '${color_by}'
    ${use_raw}
    --legend-loc '${legend_loc}'
    --legend-fontsize '${legend_fontsize}'
#if $point_size
    --size ${point_size}
#end if
    @PLOT_OPTS@
]]></command>

  <inputs>
    <expand macro="input_object_params"/>
    <param name="basis" argument="--basis" type="text" label="Name of the embedding to plot"/>
    <param name="color_by" argument="--color" type="text" label="Color by attributes, comma separated texts"/>
    <param name="use_raw" argument="--use-raw" type="boolean" checked="true" truevalue="--use-raw" falsevalue="--no-raw" label="Use raw attributes if present"/>
    <param name="legend_loc" argument="--legend-loc" type="select" label="Location of legend">
      <option value="right margin" selected="true">Right margin</option>
      <option value="on data">On data</option>
    </param>
    <param name="legend_fontsize" argument="--legend-fontsize" type="integer" value="15" label="Legend font size"/>
    <param name="point_size" argument="--size" type="integer" optional="true" label="Point size"
           help="Automatically determined if not specified."/>
    <expand macro="output_plot_params"/>
  </inputs>

  <outputs>
    <data name="output_png" format="png" from_work_dir="output.png" label="${tool.name} on ${on_string}: embedding plot"/>
  </outputs>

  <tests>
    <test>
      <param name="input_obj_file" value="find_cluster.h5"/>
      <param name="input_format" value="anndata"/>
      <param name="color_by" value="louvain"/>
      <output name="output_png" file="run_tsne.png" ftype="png" compare="sim_size"/>
    </test>
  </tests>

  <help><![CDATA[
=============================================================
Plot embeddings of a given method of dimensionality reduction
=============================================================

It yields a scatter plot in png format, wherein cells are placed in space of
reduced dimensionality and coloured by attribute of choice.

Requires calculating the specified embeddings first. For example, to make
UMAP/TSNE plots, run `Scanpy RunUMAP`/`Scanpy RunTSNE` first, then enter
"umap"/"tsne" as the name of the embedding to plot here.

@HELP@

@VERSION_HISTORY@
]]></help>
  <expand macro="citations"/>
</tool>