annotate scanpy-plot-trajectory.xml @ 16:5dde9b3c169e draft

"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 473f6f3175ac65391193d5671abaf1b560e4c593-dirty"
author ebi-gxa
date Tue, 23 Mar 2021 18:13:46 +0000
parents c646bd10dcab
children 2fe4b4cba312
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1 <?xml version="1.0" encoding="utf-8"?>
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2 <tool id="scanpy_plot_trajectory" name="Scanpy PlotTrajectory" version="@TOOL_VERSION@+galaxy1" profile="@PROFILE@">
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3 <description>visualise cell trajectories</description>
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4 <macros>
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5 <import>scanpy_macros2.xml</import>
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6 </macros>
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7 <expand macro="requirements"/>
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8 <command detect_errors="exit_code"><![CDATA[
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9 ln -s '${input_obj_file}' input.h5 &&
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10 PYTHONIOENCODING=utf-8 scanpy-cli plot paga
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11 #if $use_key
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12 --use-key '${use_key}'
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13 #end if
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14 --layout ${layout}
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15 --legend-loc '${legend_loc}'
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16 #if $basis
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17 --basis ${basis}
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18 #end if
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19 #if $settings.default == "false"
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20 --threshold ${settings.threshold}
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21 #if $settings.root
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22 --root ${settings.root}
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23 #end if
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24 ${settings.single_component}
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25 #if $settings.solid_edges
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26 --solid-edges ${settings.solid_edges}
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27 #end if
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28 #if $settings.color
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29 --color '${settings.color}'
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30 #end if
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31 #if $settings.node_size
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32 --node-size-scale ${settings.node_size}
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33 #end if
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34 #if $settings.node_font
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35 --fontsize ${settings.node_font}
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36 #end if
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37 #if $settings.edge_width
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38 --edge-width-scale ${settings.edge_width}
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39 #end if
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40 #end if
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41 @INPUT_OPTS@
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42 @PLOT_OPTS@
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43 ]]></command>
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44
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45 <inputs>
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46 <expand macro="input_object_params"/>
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47 <param name="use_key" argument="--use-key" type="text" value="paga"
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48 label="The key in `.uns` that contains trajectory information"/>
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49 <param name="layout" argument="--layout" type="select" label="Layout functions">
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50 <option value="fa">ForceAtlas2</option>
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51 <option value="fr" selected="true">Fruchterman-Reingold</option>
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52 <option value="rt">Reingold-Tilford</option>
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53 </param>
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54 <param name="legend_loc" argument="--legend-loc" type="select" label="Location of legend">
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55 <option value="right margin" selected="true">Right margin</option>
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56 <option value="on data">On data</option>
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57 </param>
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58 <param name="basis" argument="--basis" type="text" optional="true" label="Name of the embedding to plot"
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59 help="Must be a key of `.obsm` without the prefix 'X_', e.g. 'umap'."/>
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60 <expand macro="output_plot_params"/>
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61 <conditional name="settings">
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62 <param name="default" type="boolean" checked="true" label="Use programme defaults"/>
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63 <when value="true"/>
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64 <when value="false">
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65 <param name="threshold" argument="--threshold" type="float" min="0" value="0.01"
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66 label="Do not draw edges with weights below this threshold"
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67 help="Set to 0 to include all edges."/>
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68 <param name="root" argument="--root" type="integer" min="0" value="0"
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69 label="The index of the root node when choosing a tree layout"/>
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70 <param name="single_component" argument="--single-component" type="boolean" truevalue="--single-component" falsevalue="" checked="false"
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71 label="Restrict to the largest connected component"/>
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72 <param name="solid_edges" argument="--solid-edges" type="select" label="Edges to be drawn in solid black">
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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73 <option value="connectivities" selected="true">connectivities</option>
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74 <option value="connectivities_tree">connectivities_tree</option>
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75 </param>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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76 <param name="color" argument="--color" type="text" label="Name of cell annotation or gene that is used to color the nodes"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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77 <param name="node_size" argument="--node-size-scale" type="float" value="1.0"
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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78 label="Increase or decrease the size of the nodes"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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79 <param name="node_font" argument="--fontsize" type="integer" min="1" optional="true"
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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80 label="Font size for the node labels"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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81 <param name="edge_width" argument="--edge-width-scale" type="float" value="1.0"
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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82 label="Increase or decrease the width of the edges"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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83 </when>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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84 </conditional>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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85 </inputs>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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86
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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87 <outputs>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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88 <data name="output_png" format="png" from_work_dir="output.png" label="${tool.name} on ${on_string}: trajectory plot"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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89 </outputs>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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90
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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91 <tests>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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92 <test>
11
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93 <param name="input_obj_file" value="paga.h5"/>
0
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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94 <param name="input_format" value="anndata"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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95 <param name="type" value="paga_compare"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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96 <output name="output_png" file="paga_compare.png" ftype="png" compare="sim_size"/>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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97 </test>
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98 </tests>
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99
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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100 <help><![CDATA[
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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101 ===============================================================
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102 Plot PAGA-inferred trajectories (`scanpy.pl.paga/paga_compare`)
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103 ===============================================================
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104
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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105 Depending on the selected options, it yields a plot of a graph representing
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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106 inferred trajectory, or a trajectory graph side-by-side with a scatter plot of
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107 cells embedded to space of reduced dimensionality in png format.
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108
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109 It requires running PAGA, first.
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110
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111 @HELP@
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112
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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113 @VERSION_HISTORY@
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114 ]]></help>
f76e8237073a "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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115 <expand macro="citations"/>
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116 </tool>