Mercurial > repos > ebi-gxa > scanpy_read_10x
diff scanpy-read-10x.xml @ 1:3faf577f3e40 draft
"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
author | ebi-gxa |
---|---|
date | Mon, 16 Sep 2019 08:19:16 -0400 |
parents | 572b6e3b2aff |
children | f180f7230cdb |
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--- a/scanpy-read-10x.xml Wed Apr 03 11:11:38 2019 -0400 +++ b/scanpy-read-10x.xml Mon Sep 16 08:19:16 2019 -0400 @@ -2,24 +2,33 @@ <tool id="scanpy_read_10x" name="Scanpy Read10x" version="@TOOL_VERSION@+galaxy0"> <description>into hdf5 object handled by scanpy</description> <macros> - <import>scanpy_macros.xml</import> + <import>scanpy_macros2.xml</import> </macros> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ ln -s '${matrix}' matrix.mtx && ln -s '${genes}' genes.tsv && ln -s '${barcodes}' barcodes.tsv && -PYTHONIOENCODING=utf-8 scanpy-read-10x.py - -d ./ - -o read_10x.h5 - -F '${output_format}' - -v '${var_names}' +PYTHONIOENCODING=utf-8 scanpy-read-10x + --input-10x-mtx ./ + --var-names '${var_names}' +#if $cell_meta: + --extra-obs '${cell_meta}' +#end if +#if $gene_meta: + --extra-var '${gene_meta}' +#end if + @OUTPUT_OPTS@ ]]></command> <inputs> <param name="matrix" type="data" format="txt" label="Expression matrix in sparse matrix format (.mtx)"/> <param name="genes" type="data" format="tsv,tabular" label="Gene table"/> <param name="barcodes" type="data" format="tsv,tabular" label="Barcode/cell table"/> + <param name="cell_meta" type="data" format="tsv,tabular" label="Cell metadata table" optional="true" + help="Requires a header row and index column that matches the barcode/cell table"/> + <param name="gene_meta" type="data" format="tsv,tabular" label="Gene metadata table" optional="true" + help="Requires a header row and index column that matches the gene table"/> <expand macro="output_object_params"/> <param name="var_names" type="select" label="Attribute used as annotation index"> <option value="gene_ids" selected="true">Gene ID</option> @@ -28,7 +37,7 @@ </inputs> <outputs> - <data name="output_h5" format="h5" from_work_dir="read_10x.h5" label="${tool.name} on ${on_string}: ${output_format}"/> + <data name="output_h5" format="h5" from_work_dir="output.h5" label="${tool.name} on ${on_string}: ${output_format}"/> </outputs> <tests> @@ -36,15 +45,16 @@ <param name="matrix" value="matrix.mtx"/> <param name="genes" value="genes.tsv"/> <param name="barcodes" value="barcodes.tsv"/> + <!-- <param name="cell_meta" value=""/> --> <param name="output_format" value="anndata"/> - <output name="output_h5" file="read_10x.h5" ftype="h5" compare="sim_size"/> + <output name="output_h5" file="output.h5" ftype="h5" compare="sim_size"/> </test> </tests> <help><![CDATA[ -========================================================== -Read 10x-Genomics-formatted mtx directory (`read_10x_mtx`) -========================================================== +================================================================= +Read 10x-Genomics-formatted mtx directory (`scanpy.read_10x_mtx`) +================================================================= The mtx directory should contain: @@ -54,8 +64,9 @@ 3) A barcode/cell table of at least one column giving the barcode/cell IDs. -The above-mentioned files can be obtained by running `EBI SCXA Data Retrieval` -with a dataset accession. +The above-mentioned files can be obtained by running `EBI SCXA Data Retrieval` with a dataset accession or `Human Cell Atlas Matrix Downloader` with a project name/label/UUID. + +Additionally, cell and/or gene metadata table can be provided as tab-separated text with a header row and an index column that matches the respective barcode/cell and/or gene table. @HELP@