Mercurial > repos > ebi-gxa > scanpy_read_10x
diff scanpy-read-10x.xml @ 0:572b6e3b2aff draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
author | ebi-gxa |
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date | Wed, 03 Apr 2019 11:11:38 -0400 |
parents | |
children | 3faf577f3e40 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/scanpy-read-10x.xml Wed Apr 03 11:11:38 2019 -0400 @@ -0,0 +1,66 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="scanpy_read_10x" name="Scanpy Read10x" version="@TOOL_VERSION@+galaxy0"> + <description>into hdf5 object handled by scanpy</description> + <macros> + <import>scanpy_macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +ln -s '${matrix}' matrix.mtx && +ln -s '${genes}' genes.tsv && +ln -s '${barcodes}' barcodes.tsv && +PYTHONIOENCODING=utf-8 scanpy-read-10x.py + -d ./ + -o read_10x.h5 + -F '${output_format}' + -v '${var_names}' +]]></command> + + <inputs> + <param name="matrix" type="data" format="txt" label="Expression matrix in sparse matrix format (.mtx)"/> + <param name="genes" type="data" format="tsv,tabular" label="Gene table"/> + <param name="barcodes" type="data" format="tsv,tabular" label="Barcode/cell table"/> + <expand macro="output_object_params"/> + <param name="var_names" type="select" label="Attribute used as annotation index"> + <option value="gene_ids" selected="true">Gene ID</option> + <option value="gene_symbols">Gene symbol</option> + </param> + </inputs> + + <outputs> + <data name="output_h5" format="h5" from_work_dir="read_10x.h5" label="${tool.name} on ${on_string}: ${output_format}"/> + </outputs> + + <tests> + <test> + <param name="matrix" value="matrix.mtx"/> + <param name="genes" value="genes.tsv"/> + <param name="barcodes" value="barcodes.tsv"/> + <param name="output_format" value="anndata"/> + <output name="output_h5" file="read_10x.h5" ftype="h5" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +========================================================== +Read 10x-Genomics-formatted mtx directory (`read_10x_mtx`) +========================================================== + +The mtx directory should contain: + +1) Raw expression quantification as a sparse matrix in Matrix Market format, where the each column is a gene and each row is a barcode/cell. + +2) A gene table of at least two columns where the first column gives the gene IDs. + +3) A barcode/cell table of at least one column giving the barcode/cell IDs. + +The above-mentioned files can be obtained by running `EBI SCXA Data Retrieval` +with a dataset accession. + + +@HELP@ + +@VERSION_HISTORY@ +]]></help> + <expand macro="citations"/> +</tool>