Mercurial > repos > ebi-gxa > scanpy_run_paga
diff scanpy-run-paga.xml @ 0:3e53344e540d draft
"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
author | ebi-gxa |
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date | Mon, 16 Sep 2019 08:25:36 -0400 |
parents | |
children | 09c82810d14a |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/scanpy-run-paga.xml Mon Sep 16 08:25:36 2019 -0400 @@ -0,0 +1,65 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="scanpy_run_paga" name="Scanpy PAGA" version="@TOOL_VERSION@+galaxy0"> + <description>trajectory inference</description> + <macros> + <import>scanpy_macros2.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ +ln -s '${input_obj_file}' input.h5 && +PYTHONIOENCODING=utf-8 scanpy-cli paga + --groups '${groups}' +#if $use_graph + --use-graph '${use_graph}' +#end if +#if $key_added + --key-added '${key_added}' +#end if + @INPUT_OPTS@ + @OUTPUT_OPTS@ +]]></command> + + <inputs> + <expand macro="input_object_params"/> + <expand macro="output_object_params"/> + + <param name="groups" argument="--groups" type="text" label="Name of the clustering"/> + <param name="use_graph" argument="--use-graph" value="neighbors" type="text" label="Name of the slot that holds the KNN graph"/> + <param name="key_added" argument="--key-added" type="text" optional="true" label="Additional suffix to the name of the slot to save the calculated trajectory"/> + </inputs> + + <outputs> + <data name="output_h5" format="h5" from_work_dir="output.h5" label="${tool.name} on ${on_string}: PAGA object"/> + </outputs> + + <tests> + <test> + <param name="input_obj_file" value="compute_graph.h5"/> + <param name="input_format" value="anndata"/> + <param name="output_format" value="anndata"/> + <param name="groups" value="leiden"/> + <param name="use_graph" value="neighbors"/> + <output name="output_h5" file="paga.h5" ftype="h5" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +=================================================================== +Perform PAGA (partition-based graph abstraction) (`scanpy.tl.paga`) +=================================================================== + +Infer trajectories by mapping out the coarse-grained connectivity structures of +complex manifolds of single cell KNN graphs (Wolf et al, 2019). + +This requires to run `Scanpy FindCluster`, first. + +It yields `paga`, connectivity graph between clusters. + +@HELP@ + +@VERSION_HISTORY@ +]]></help> + <expand macro="citations"> + <citation type="doi">10.1186/s13059-019-1663-x</citation> + </expand> +</tool>