Mercurial > repos > ebi-gxa > seurat_find_markers
diff seurat_find_markers.xml @ 1:7895b4bb6247 draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit 0463f230d18201c740851d72e31a5024f391207f
author | ebi-gxa |
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date | Mon, 25 Nov 2019 06:09:57 -0500 |
parents | fde95fe95f15 |
children | 4039a99ae846 |
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--- a/seurat_find_markers.xml Wed Apr 03 11:17:29 2019 -0400 +++ b/seurat_find_markers.xml Mon Nov 25 06:09:57 2019 -0500 @@ -1,4 +1,4 @@ -<tool id="seurat_find_markers" name="Seurat FindMarkers" version="2.3.1+galaxy1"> +<tool id="seurat_find_markers" name="Seurat FindMarkers" version="@SEURAT_VERSION@_@VERSION@+galaxy0"> <description>find markers (differentially expressed genes)</description> <macros> <import>seurat_macros.xml</import> @@ -8,7 +8,7 @@ <command detect_errors="exit_code"><![CDATA[ seurat-find-markers.R ---input-object-file '$input' +@INPUT_OBJECT@ --output-text-file output.txt #if $genes_use: @@ -49,7 +49,7 @@ ]]></command> <inputs> - <param name="input" type="data" format="rdata" label="RDS object" /> + <expand macro="input_object_params"/> <expand macro="genes-use-input"/> <param name="logfc_threshold" label="LogFC logfc_threshold" optional="true" argument="--logfc-threshold" type="float" help="Limit testing to genes which show, on average, at least X-fold difference (log-scale) between the two groups of cells. Default is 0.25 Increasing logfc.threshold speeds up the function, but can miss weaker signals."/> <param name="max_cells_per_ident" label="Max cells per ident" optional="true" argument="--max-cells-per-ident" type="integer" help="Down sample each identity class to a max number. Default is no downsampling. Not activated by default (set to Inf)."/> @@ -74,7 +74,7 @@ </inputs> <outputs> - <data name="output" format="txt" from_work_dir="output.txt" label="${tool.name} on ${on_string}: Text file"/> + <data name="output" format="csv" from_work_dir="output.txt" label="${tool.name} on ${on_string}: Text file"/> </outputs> <tests> @@ -88,12 +88,17 @@ **What it does** -Seurat_ is a toolkit for quality control, analysis, and exploration of single cell RNA sequencing data. -It is developed and maintained by the `Satija Lab`_ at NYGC. Seurat aims to enable users to identify and -interpret sources of heterogeneity from single cell transcriptomic measurements, and to integrate diverse -types of single cell data. +This tool finds markers (differentially expressed genes) for each of the identity classes in a dataset. +It outputs a text file containing a ranked list of putative markers, and associated statistics (p-values, ROC score, etc.) -This tool finds markers (differentially expressed genes) for each of the identity classes in a dataset. It outputs a text file containing a ranked list of putative markers, and associated statistics (p-values, ROC score, etc.) +p-value adjustment is performed using bonferroni correction based on the total +number of genes in the dataset. Other correction methods are not recommended, +as Seurat pre-filters genes using the arguments above, reducing the number of +tests performed. Lastly, as Aaron Lun has pointed out, p-values should be +interpreted cautiously, as the genes used for clustering are the same genes +tested for differential expression. + +@SEURAT_INTRO@ -----