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changeset 6:07fc9f4841b3 draft default tip

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planemo upload commit 0264c359f1d638bbbbab515a3502231f679cdcf6
author ebi-gxa
date Sat, 02 Mar 2024 10:42:01 +0000
parents 3dd20b758756
children
files extra/macro_mapper_seurat.yaml get_test_data.sh scripts/seurat-scale-data.R seurat_find_markers.xml seurat_macros.xml
diffstat 5 files changed, 518 insertions(+), 16 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/extra/macro_mapper_seurat.yaml	Sat Mar 02 10:42:01 2024 +0000
@@ -0,0 +1,90 @@
+---
+- option_group:
+    - input-object-file
+    - input-format
+  pre_command_macros:
+    - INPUT_OBJ_PREAMBLE
+  post_command_macros:
+    - INPUT_OBJECT
+  input_declaration_macros:
+    - input_object_params
+- option_group:
+    - output-object-file
+    - output-format
+  post_command_macros:
+    - OUTPUT_OBJECT
+  input_declaration_macros:
+    - output_object_params
+  output_declaration_macros:
+    - output_files
+- option_group:
+    - input-object-files
+    - input-format
+  pre_command_macros:
+    - INPUT_OBJS_PREAMBLE
+  post_command_macros:
+    - INPUT_OBJECTS
+  input_declaration_macros:
+    - input_object_params:
+        multiple: true
+- option_group:
+    - reference-object-files
+    - reference-format
+  pre_command_macros:
+    - REFERENCE_OBJS_PREAMBLE
+  post_command_macros:
+    - REFERENCE_OBJECTS
+  input_declaration_macros:
+    - input_object_params:
+        varname: reference
+        multiple: true
+        optional: true
+- option_group:
+    - reference-object-file
+    - reference-format
+  pre_command_macros:
+    - REFERENCE_OBJ_PREAMBLE
+  post_command_macros:
+    - REFERENCE_OBJECT
+  input_declaration_macros:
+    - input_object_params:
+        varname: reference
+- option_group:
+    - anchors-object-file
+    - anchors-format
+  pre_command_macros:
+    - ANCHORS_OBJ_PREAMBLE
+  post_command_macros:
+    - ANCHORS_OBJECT
+  input_declaration_macros:
+    - input_object_params:
+        varname: anchors
+- option_group:
+    - query-object-file
+    - query-format
+  pre_command_macros:
+    - QUERY_OBJ_PREAMBLE
+  post_command_macros:
+    - QUERY_OBJECT
+  input_declaration_macros:
+    - input_object_params:
+        varname: query
+- option_group:
+    - plot-out
+  post_command_macros:
+    - OUTPUT_PLOT
+  output_declaration_macros:
+    - plot_output_files_format:
+        format: png
+    - plot_output_files_format:
+        format: pdf
+    - plot_output_files_format:
+        format: eps
+    - plot_output_files_format:
+        format: jpg
+    - plot_output_files_format:
+        format: ps
+    - plot_output_files_format:
+        format: tiff
+    - plot_output_files_format:
+        format: svg
--- a/get_test_data.sh	Fri Mar 04 07:26:16 2022 +0000
+++ b/get_test_data.sh	Sat Mar 02 10:42:01 2024 +0000
@@ -15,8 +15,23 @@
 
 LOOM_LINK='https://drive.google.com/uc?export=download&id=1qNk5cg8hJG3Nv1ljTKmUEnxTOf11EEZX'
 H5AD_LINK='https://drive.google.com/uc?export=download&id=1YpE0H_t_dkh17P-WBhPijKvRiGP0BlBz'
+
+H5AD_SC182_LINK='https://drive.google.com/uc?export=download&id=16PUJ2KAkXT8F1UkfqU-9LWoOJUkUG1rp'
 SCE_LINK='https://drive.google.com/uc?export=download&id=1UKdyf3M01uAt7oBg93JfmRvNVB_jlUKe'
 
+# Seurat v4 exclusives
+IFNB_BASE_FILE='ifnb_'
+
+IFNB_CTRL_INT_LINK='https://drive.google.com/uc?export=download&id=15E_MLz-UclJYInNaA7YKLhLo5W-qlykL'
+IFNB_STIM_INT_LINK='https://drive.google.com/uc?export=download&id=14iKgCJGPk16dEmpJJF-Gp_lBDcOdo-54'
+
+## Classify and UMAP mapping
+CLASSIFY_QUERY_LINK='https://oc.ebi.ac.uk/s/MlEDILFYRrvkS6E/download'
+CLASSIFY_RESULTS_ANCHORS_OBJECT_LINK='https://drive.google.com/uc?export=download&id=1Xtv4K_CxIU1cJ8RjJ7NTvzLQkLvc8a3i'
+# UMAP_RESULT_OBJECT_LINK='https://oc.ebi.ac.uk/s/k4MdM07y9DAnurp/download'
+UMAP_RESULT_OBJECT_LINK='https://oc.ebi.ac.uk/s/D1z4z2ef1e3dyc3/download'
+
+
 function get_data {
   local link=$1
   local fname=$2
@@ -28,6 +43,7 @@
 }
 
 # get matrix data
+mkdir -p test-data
 pushd test-data
 get_data $MTX_LINK mtx.zip
 unzip mtx.zip
@@ -49,3 +65,13 @@
 get_data $LOOM_LINK $BASENAME_FILE"_loom.h5"
 get_data $SCE_LINK $BASENAME_FILE"_sce.rds"
 get_data $H5AD_LINK $BASENAME_FILE".h5ad"
+
+get_data $H5AD_SC182_LINK $BASENAME_FILE"_sc182.h5ad"
+
+get_data $IFNB_CTRL_INT_LINK $IFNB_BASE_FILE"ctrl_norm_fvg.rds"
+get_data $IFNB_STIM_INT_LINK $IFNB_BASE_FILE"stim_norm_fvg.rds"
+
+get_data $CLASSIFY_QUERY_LINK "Classify_query.rds"
+get_data $CLASSIFY_RESULTS_ANCHORS_OBJECT_LINK "Classify_anchors.rds"
+get_data $UMAP_RESULT_OBJECT_LINK "UMAP_result_integrated.rds"
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/scripts/seurat-scale-data.R	Sat Mar 02 10:42:01 2024 +0000
@@ -0,0 +1,165 @@
+#!/usr/bin/env Rscript 
+
+# Load optparse we need to check inputs
+
+suppressPackageStartupMessages(require(optparse))
+
+# Load common functions
+
+suppressPackageStartupMessages(require(workflowscriptscommon))
+
+# parse options
+
+option_list = list(
+  make_option(
+    c("-i", "--input-object-file"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "File name in which a serialized R matrix object may be found."
+  ),
+  make_option(
+    c("--input-format"),
+    action = "store",
+    default = "seurat",
+    type = 'character',
+    help = "Either loom, seurat, anndata or singlecellexperiment for the input format to read."
+  ),
+  make_option(
+    c("--output-format"),
+    action = "store",
+    default = "seurat",
+    type = 'character',
+    help = "Either loom, seurat, anndata or singlecellexperiment for the output format."
+  ),
+  make_option(
+    c("-e", "--genes-use"),
+    action = "store",
+    default = NULL,
+    type = 'character',
+    help = "File with gene names to scale/center (one gene per line). Default is all genes in object@data."
+  ),
+  make_option(
+    c("-v", "--vars-to-regress"),
+    action = "store",
+    default = NULL,
+    type = 'character',
+    help = "Comma-separated list of variables to regress out (previously latent.vars in RegressOut). For example, nUMI, or percent.mito."
+  ),
+  make_option(
+    c("-m", "--model-use"),
+    action = "store",
+    default = 'linear',
+    type = 'character',
+    help = "Use a linear model or generalized linear model (poisson, negative binomial) for the regression. Options are 'linear' (default), 'poisson', and 'negbinom'."
+  ),
+  make_option(
+    c("-u", "--use-umi"),
+    action = "store",
+    default = FALSE,
+    type = 'logical',
+    help = "Regress on UMI count data. Default is FALSE for linear modeling, but automatically set to TRUE if model.use is 'negbinom' or 'poisson'."
+  ),
+  make_option(
+    c("-s", "--do-not-scale"),
+    action = "store_true",
+    default = FALSE,
+    type = 'logical',
+    help = "Skip the data scale."
+  ),
+  make_option(
+    c("-c", "--do-not-center"),
+    action = "store_true",
+    default = FALSE,
+    type = 'logical',
+    help = "Skip data centering."
+  ),  
+  make_option(
+    c("-x", "--scale-max"),
+    action = "store",
+    default = 10,
+    type = 'double',
+    help = "Max value to return for scaled data. The default is 10. Setting this can help reduce the effects of genes that are only expressed in a very small number of cells. If regressing out latent variables and using a non-linear model, the default is 50."
+  ),
+  make_option(
+    c("-b", "--block-size"),
+    action = "store",
+    default = 1000,
+    type = 'integer',
+    help = "Default size for number of genes to scale at in a single computation. Increasing block.size may speed up calculations but at an additional memory cost."
+  ),
+  make_option(
+    c("-d", "--min-cells-to-block"),
+    action = "store",
+    default = 1000,
+    type = 'integer',
+    help = "If object contains fewer than this number of cells, don't block for scaling calculations."
+  ),
+  make_option(
+    c("-n", "--check-for-norm"),
+    action = "store",
+    default = TRUE,
+    type = 'logical',
+    help = "Check to see if data has been normalized, if not, output a warning (TRUE by default)."
+  ),
+  make_option(
+    c("-o", "--output-object-file"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "File name in which to store serialized R object of type 'Seurat'.'"
+  )
+)
+
+opt <- wsc_parse_args(option_list, mandatory = c('input_object_file', 'output_object_file'))
+
+# Check parameter values
+
+if ( ! file.exists(opt$input_object_file)){
+  stop((paste('File', opt$input_object_file, 'does not exist')))
+}
+
+if (! is.null(opt$genes_use)){
+  if (! file.exists(opt$genes_use)){
+    stop((paste('Supplied genes file', opt$genes_use, 'does not exist')))
+  }else{
+    genes_use <- readLines(opt$genes_use)
+  }
+}else{
+  genes_use <- NULL
+}
+
+# break up opt$vars_to_regress into a list if it has commas
+opt$vars_to_regress <- unlist(strsplit(opt$vars_to_regress, ","))
+
+# Now we're hapy with the arguments, load Seurat and do the work
+
+suppressPackageStartupMessages(require(Seurat))
+if(opt$input_format == "loom" | opt$output_format == "loom") {
+  suppressPackageStartupMessages(require(SeuratDisk))
+} else if(opt$input_format == "singlecellexperiment" | opt$output_format == "singlecellexperiment") {
+  suppressPackageStartupMessages(require(scater))
+}
+
+# Input from serialized R object
+
+seurat_object <- read_seurat4_object(input_path = opt$input_object_file, format = opt$input_format)
+# https://stackoverflow.com/questions/9129673/passing-list-of-named-parameters-to-function
+# might be useful
+scaled_seurat_object <- ScaleData(seurat_object, 
+                                  features = genes_use, 
+                                  vars.to.regress = opt$vars_to_regress, 
+                                  model.use = opt$model_use, 
+                                  use.umi = opt$use_umi, 
+                                  do.scale = !opt$do_not_scale, 
+                                  do.center = !opt$do_not_center, 
+                                  scale.max = opt$scale_max, 
+                                  block.size = opt$block_size, 
+                                  min.cells.to.block = opt$min_cells_to_block, 
+                                  verbose = FALSE)
+
+
+# Output to a serialized R object
+write_seurat4_object(seurat_object = scaled_seurat_object, 
+                     output_path = opt$output_object_file,
+                     format = opt$output_format)
--- a/seurat_find_markers.xml	Fri Mar 04 07:26:16 2022 +0000
+++ b/seurat_find_markers.xml	Sat Mar 02 10:42:01 2024 +0000
@@ -82,7 +82,7 @@
             <param name="rds_seurat_file" ftype="rdata" value="E-MTAB-6077-3k_features_90_cells-clusters.rds"/>
             <output name="output" >
               <assert_contents>
-                <has_n_lines n="1038" />
+                <has_n_lines n="1130" />
               </assert_contents>
             </output>
         </test>
--- a/seurat_macros.xml	Fri Mar 04 07:26:16 2022 +0000
+++ b/seurat_macros.xml	Sat Mar 02 10:42:01 2024 +0000
@@ -1,7 +1,7 @@
 <?xml version="1.0"?>
 <macros>
-    <token name="@VERSION@">0.3.0</token>
-    <token name="@SEURAT_VERSION@">3.2.3</token>
+    <token name="@VERSION@">4.0.0</token>
+    <token name="@SEURAT_VERSION@">4.0.4</token>
     <xml name="requirements">
         <requirements>
             <requirement type="package" version="@VERSION@">seurat-scripts</requirement>
@@ -13,44 +13,237 @@
     ]]></version_command>
     </xml>
 
-   <xml name="input_object_params">
-     <conditional name="input" label="Input format">
-       <param type="select" name="format" label="Choose the format of the input" help="RData, Loom or AnnData">
+   <xml name="input_object_params" token_multiple="False" token_varname="input" token_optional="False">
+     <conditional name="@VARNAME@" label="Input format">
+       <param type="select" name="format" label="Choose the format of the @VARNAME@" help="Seurat RDS, Seurat H5, Single Cell Experiment RDS, Loom or AnnData">
          <option value="rds_seurat" selected="true">RDS with a Seurat object</option>
          <option value="loom">Loom</option>
+         <option value="h5seurat">Seurat HDF5</option>
          <option value="anndata">AnnData</option>
          <option value="rds_sce">RDS with a Single Cell Experiment object</option>
        </param>
        <when value="anndata">
-         <param type="data" name="anndata_file" label="AnnData file" help="The AnnData format provided by Scanpy" format="h5,h5ad"/>
+         <param type="data" name="anndata_file" multiple="@MULTIPLE@" optional="@OPTIONAL@" label="AnnData file" help="Select AnnData files for @VARNAME@" format="h5,h5ad"/>
        </when>
        <when value="loom">
-         <param type="data" name="loom_file" label="Loom file" help="Input as Loom v? file" format="h5,h5loom"/>
+         <param type="data" name="loom_file" multiple="@MULTIPLE@" optional="@OPTIONAL@" label="Loom file" help="Select Loom file(s) for @VARNAME@" format="h5,h5loom"/>
        </when>
        <when value="rds_seurat">
-         <param type="data" name="rds_seurat_file" label="RDS file" help="Input as RDS file with Seurat 3 object" format="rdata"/>
+         <param type="data" name="rds_seurat_file" multiple="@MULTIPLE@" optional="@OPTIONAL@" label="RDS file" help="Select RDS file(s) with Seurat object for @VARNAME@" format="rdata"/>
        </when>
        <when value="rds_sce">
-         <param type="data" name="rds_sce_file" label="RDS file" help="Input as RDS file with Single Cell Experiment object" format="rdata"/>
+         <param type="data" name="rds_sce_file" multiple="@MULTIPLE@" optional="@OPTIONAL@" label="RDS file" help="Select RDS file(s) with Single Cell Experiment object for @VARNAME@" format="rdata"/>
+       </when>
+       <when value="h5seurat">
+         <param type="data" name="h5seurat_file" multiple="@MULTIPLE@" optional="@OPTIONAL@" label="Seurat HDF5" help="Select Seurat HDF5 file(s) for @VARNAME" format="h5"/>
        </when>
      </conditional>
    </xml>
 
+   <token name="@INPUT_OBJ_PREAMBLE@">
+     #if $input.format == 'loom'
+     ln -s '$input.loom_file' input.loom;
+     #else if $input.format == 'h5seurat'
+     ln -s '$input.h5seurat_file' input.h5seurat;
+     #else if $input.format == 'anndata'
+     ## it complains when using links for AnnData...
+     cp '$input.anndata_file' input.h5ad;
+     #end if
+   </token>
+
    <token name="@INPUT_OBJECT@">
     #if $input.format == "anndata"
-        --input-object-file '$input.anndata_file' --input-format anndata
+        --input-object-file input.h5ad --input-format anndata
     #else if $input.format == "loom"
-        --input-object-file '$input.loom_file' --input-format loom
+        --input-object-file input.loom --input-format loom
     #else if $input.format == "rds_seurat"
         --input-object-file '$input.rds_seurat_file' --input-format seurat
     #else if $input.format == "rds_sce"
         --input-object-file '$input.rds_sce_file' --input-format singlecellexperiment
+    #else if $input.format == "h5seurat"
+        --input-object-file input.h5seurat --input-format h5seurat
+    #end if
+   </token>
+
+   <token name="@QUERY_OBJ_PREAMBLE@">
+     #if $query.format == 'loom'
+     ln -s '$query.loom_file' query.loom;
+     #else if $query.format == 'h5seurat'
+     ln -s '$query.h5seurat_file' query.h5seurat;
+     #else if $query.format == 'anndata'
+     ## it complains when using links for AnnData...
+     cp '$query.anndata_file' query.h5ad;
+     #end if
+   </token>
+
+   <token name="@QUERY_OBJECT@">
+    #if $query.format == "anndata"
+        --query-object-file query.h5ad --query-format anndata
+    #else if $query.format == "loom"
+        --query-object-file query.loom --query-format loom
+    #else if $query.format == "rds_seurat"
+        --query-object-file '$query.rds_seurat_file' --query-format seurat
+    #else if $query.format == "rds_sce"
+        --query-object-file '$query.rds_sce_file' --query-format singlecellexperiment
+    #else if $query.format == "h5seurat"
+        --query-object-file query.h5seurat --query-format h5seurat
+    #end if
+  </token>
+
+  <token name="@ANCHORS_OBJ_PREAMBLE@">
+    #if $anchors.format == 'loom'
+    ln -s '$anchors.loom_file' anchors.loom;
+    #else if $anchors.format == 'h5seurat'
+    ln -s '$anchors.h5seurat_file' anchors.h5seurat;
+    #else if $anchors.format == 'anndata'
+    ## it complains when using links for AnnData...
+    cp '$anchors.anndata_file' anchors.h5ad;
+    #end if
+  </token>
+
+  <token name="@ANCHORS_OBJECT@">
+   #if $anchors.format == "anndata"
+       --anchors-object-file anchors.h5ad --anchors-format anndata
+   #else if $anchors.format == "loom"
+       --anchors-object-file anchors.loom --anchors-format loom
+   #else if $anchors.format == "rds_seurat"
+       --anchors-object-file '$anchors.rds_seurat_file' --anchors-format seurat
+   #else if $anchors.format == "rds_sce"
+       --anchors-object-file '$anchors.rds_sce_file' --anchors-format singlecellexperiment
+   #else if $anchors.format == "h5seurat"
+       --anchors-object-file anchors.h5seurat --anchors-format h5seurat
+   #end if
+ </token>
+
+ <token name="@REFERENCE_OBJ_PREAMBLE@">
+   #if $reference.format == 'loom'
+   ln -s '$reference.loom_file' reference.loom;
+   #else if $reference.format == 'h5seurat'
+   ln -s '$reference.h5seurat_file' reference.h5seurat;
+   #else if $reference.format == 'anndata'
+   ## it complains when using links for AnnData...
+   cp '$reference.anndata_file' reference.h5ad;
+   #end if
+ </token>
+
+ <token name="@REFERENCE_OBJECT@">
+  #if $reference.format == "anndata"
+      --reference-object-file reference.h5ad --reference-format anndata
+  #else if $reference.format == "loom"
+      --reference-object-file reference.loom --reference-format loom
+  #else if $reference.format == "rds_seurat"
+      --reference-object-file '$reference.rds_seurat_file' --reference-format seurat
+  #else if $reference.format == "rds_sce"
+      --reference-object-file '$reference.rds_sce_file' --reference-format singlecellexperiment
+  #else if $reference.format == "h5seurat"
+      --reference-object-file reference.h5seurat --reference-format h5seurat
+  #end if
+ </token>
+
+   <token name="@INPUT_OBJS_PREAMBLE@">
+     #if $input.format == 'loom'
+     #for $i, $fh in enumerate($input.loom_file):
+        ln -s '$fh' input.${i}.loom;
+     #end for
+     #else if $input.format == 'h5seurat'
+     #for $i, $fh in enumerate($input.h5seurat_file):
+        ln -s '$fh' input.${i}.h5seurat;
+     #end for
+     #else if $input.format == 'anndata'
+     ## it complains when using links for AnnData...
+     #for $i, $fh in enumerate($input.anndata_file):
+        cp '$fh' input.${i}.h5ad;
+     #end for
+     #end if
+   </token>
+
+   <token name="@INPUT_OBJECTS@">
+    #if $input.format == "anndata"
+        --input-object-files
+    #set file_array = [ "input."+str($i)+".h5ad" for $i, $fh in enumerate($input.anndata_file)]
+    #set files = ",".join($file_array)
+        ${files}
+         --input-format anndata
+    #else if $input.format == "loom"
+        --input-object-files
+    #set file_array = [ "input."+str($i)+".loom" for $i, $fh in enumerate($input.loom_file)]
+    #set files = ",".join($file_array)
+        ${files}
+         --input-format loom
+    #else if $input.format == "rds_seurat"
+        --input-object-files
+    #set file_array = $input.rds_seurat_file
+    #set files = ",".join([ str($fh) for $fh in $file_array ])
+        ${files}
+        --input-format seurat
+    #else if $input.format == "rds_sce"
+        --input-object-files
+    #set file_array = $input.rds_sce_file
+    #set files = ",".join([ str($fh) for $fh in $file_array ])
+        ${files}
+        --input-format singlecellexperiment
+    #else if $input.format == "h5seurat"
+        --input-object-files
+    #set file_array = [ "input."+str($i)+".h5seurat" for $i, $fh in enumerate($input.h5seurat)]
+    #set files = ",".join($file_array)
+        ${files}
+        --input-format h5seurat
+    #end if
+   </token>
+
+   <token name="@REFERENCE_OBJS_PREAMBLE@">
+     #if $reference.format == 'loom'
+     #for $i, $fh in enumerate($reference.loom_file):
+        ln -s '$fh' reference.${i}.loom;
+     #end for
+     #else if $reference.format == 'h5seurat'
+     #for $i, $fh in enumerate($reference.h5seurat_file):
+        ln -s '$fh' reference.${i}.h5seurat;
+     #end for
+     #else if $reference.format == 'anndata'
+     ## it complains when using links for AnnData...
+     #for $i, $fh in enumerate($reference.anndata_file):
+        cp '$fh' reference.${i}.h5ad;
+     #end for
+     #end if
+   </token>
+
+   <token name="@REFERENCE_OBJECTS@">
+    #if $reference.format == "anndata" and $reference.anndata_file:
+        --reference-object-files
+    #set file_array = [ "reference."+str($i)+".h5ad" for $i, $fh in enumerate($reference.anndata_file)]
+    #set files = ",".join($file_array)
+        ${files}
+         --reference-format anndata
+    #else if $reference.format == "loom" and $reference.loom_file:
+        --reference-object-files
+    #set file_array = [ "reference."+str($i)+".loom" for $i, $fh in enumerate($reference.loom_file)]
+    #set files = ",".join($file_array)
+        ${files}
+         --reference-format loom
+    #else if $reference.format == "rds_seurat" and $reference.rds_seurat_file:
+        --reference-object-files
+    #set files = ",".join([ str($fh) for $fh in $reference.rds_seurat_file ])
+        ${files}
+        --reference-format seurat
+    #else if $reference.format == "rds_sce" and $reference.rds_sce_file:
+        --reference-object-files
+    #set files = ",".join([ str($fh) for $fh in $reference.rds_sce_file ])
+        ${files}
+        --reference-format singlecellexperiment
+    #else if $reference.format == "h5seurat" and $reference.h5seurat:
+        --reference-object-files
+    #set file_array = [ "reference."+str($i)+".h5seurat" for $i, $fh in enumerate($reference.h5seurat)]
+    #set files = ",".join($file_array)
+        ${files}
+        --reference-format h5seurat
     #end if
    </token>
 
    <xml name="output_object_params">
-     <param type="select" name="format" label="Choose the format of the output" help="Seurat, Single Cell Experiment or Loom">
+     <param type="select" name="format" label="Choose the format of the output" help="Seurat, Single Cell Experiment, AnnData or Loom">
        <option value="rds_seurat" selected="true">RDS with a Seurat object</option>
+       <option value="anndata">AnnData written by Seurat</option>
        <option value="loom">Loom</option>
        <option value="rds_sce">RDS with a Single Cell Experiment object</option>
      </param>
@@ -63,6 +256,9 @@
     <data name="rds_seurat_file" format="rdata" label="${tool.name} on ${on_string}: Seurat RDS">
       <filter>format == 'rds_seurat'</filter>
     </data>
+    <data name="anndata_file" format="h5ad" label="${tool.name} on ${on_string}: AnnData from Seurat">
+        <filter>format == 'anndata'</filter>
+    </data>
     <data name="rds_sce_file" format="rdata" label="${tool.name} on ${on_string}: Seurat Single Cell Experiment RDS">
       <filter>format == 'rds_sce'</filter>
     </data>
@@ -80,6 +276,30 @@
     #end if
    </token>
 
+   <xml name="plot_output_files_format" token_format="png">
+     <data label="Seurat ${plot_type.plot_type_selector} on ${on_string}: @FORMAT@ plot" name="plot_out_@FORMAT@" format='@FORMAT@' >
+       <filter>plot_format == '@FORMAT@'</filter>
+     </data>
+   </xml>
+
+   <token name="@OUTPUT_PLOT@">
+    #if $plot_format == "png"
+        --plot-out '$plot_out_png'
+    #else if $plot_format == "pdf"
+        --plot-out '$plot_out_pdf'
+    #else if $plot_format == "eps"
+        --plot-out '$plot_out_eps'
+    #else if $plot_format == "ps"
+        --plot-out '$plot_out_ps'
+    #else if $plot_format == "jpg"
+        --plot-out '$plot_out_jpg'
+    #else if $plot_format == "tiff"
+        --plot-out '$plot_out_tiff'
+    #else if $plot_format == "svg"
+        --plot-out '$plot_out_svg'
+    #end if
+   </token>
+
     <xml name="genes-use-input">
       <param name="genes_use" argument="--genes-use" optional="true" type="data" format="tsv,txt,tabular" label="Genes to use" help="A file with gene names to use in construction of SNN graph if building directly based on expression data rather than a dimensionally reduced representation (i.e. PCs)."/>
     </xml>
@@ -96,6 +316,7 @@
 
     <token name="@VERSION_HISTORY@"><![CDATA[
 **Version history**
+4.0.0: Moves to Seurat 4.0.0, introducing a number of methods for merging datasets, plus the whole suite of Seurat plots. Pablo Moreno with funding from AstraZeneca.
 
 3.2.3+galaxy0: Moves to Seurat 3.2.3 and introduce convert method, improving format interconversion support.
 
@@ -108,14 +329,15 @@
   Find clusters: removed dims-use, k-param, prune-snn.
 
 2.3.1+galaxy0: Improved documentation and further exposition of all script's options. Pablo Moreno, Jonathan Manning and Ni Huang, Expression Atlas team https://www.ebi.ac.uk/gxa/home  at
-EMBL-EBI https://www.ebi.ac.uk/. Parts obtained from wrappers from Christophe Antoniewski(github.com/drosofff) and Lea Bellenger(github.com/bellenger-l).
+EMBL-EBI https://www.ebi.ac.uk/. Parts obtained from wrappers from Christophe Antoniewski (GitHub drosofff) and Lea Bellenger (GitHub bellenger-l).
 
-0.0.1: Initial contribution. Maria Doyle (github.com/mblue9).
+0.0.1: Initial contribution. Maria Doyle (GitHub mblue9).
       ]]></token>
 
 
     <xml name="citations">
         <citations>
+            <citation type="doi">10.1038/s41592-021-01102-w</citation>
             <citation type="doi">10.1038/nbt.4096</citation>
             <citation type="bibtex">
               @misc{r-seurat-scripts.git,
@@ -127,7 +349,6 @@
               url = {https://github.com/ebi-gene-expression-group/r-seurat-scripts.git},
             }
             </citation>
-            <citation type="doi">10.1038/s41592-021-01102-w</citation>
         </citations>
     </xml>
 </macros>