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| author | ebi-gxa |
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| date | Sat, 02 Mar 2024 10:40:57 +0000 |
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| -1:000000000000 | 0:699c0ca328f2 |
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| 1 <tool id="seurat_select_integration_features" name="Seurat select integration features" profile="18.01" version="@SEURAT_VERSION@+galaxy0"> | |
| 2 <description>from a list of studies</description> | |
| 3 <macros> | |
| 4 <import>seurat_macros.xml</import> | |
| 5 </macros> | |
| 6 <expand macro="requirements" /> | |
| 7 <expand macro="version" /> | |
| 8 <command detect_errors="exit_code"><![CDATA[ | |
| 9 @INPUT_OBJS_PREAMBLE@ | |
| 10 seurat-select-integration-features.R | |
| 11 @INPUT_OBJECTS@ | |
| 12 | |
| 13 --nfeatures '$nfeatures' | |
| 14 | |
| 15 | |
| 16 #if $assay_list | |
| 17 --assay-list '$assay_list' | |
| 18 #end if | |
| 19 | |
| 20 $verbose | |
| 21 | |
| 22 | |
| 23 #if $fvf_nfeatures | |
| 24 --fvf-nfeatures '$fvf_nfeatures' | |
| 25 #end if | |
| 26 | |
| 27 | |
| 28 --file-out '$file_out' | |
| 29 | |
| 30 | |
| 31 ]]></command> | |
| 32 <inputs> | |
| 33 <expand macro="input_object_params" multiple="True" /> | |
| 34 <param label="Number of features" optional='true' value='2000' name="nfeatures" argument="--nfeatures" type="integer" help="Number of features to return"/> | |
| 35 <param label="Assay-list" optional='true' name="assay_list" argument="--assay-list" type="text" help="Name or vector of assay names (one for each object) from which to pull the variable features."/> | |
| 36 <param label="Verbose" optional='true' value='true' name="verbose" argument="--do-not-verbose" type="boolean" truevalue='' falsevalue='--do-not-verbose' checked='true' help="Print messages"/> | |
| 37 <param label="Fvf nfeatures" optional='true' value='2000' name="fvf_nfeatures" argument="--fvf-nfeatures" type="integer" help="nfeatures for FindVariableFeatures. Used if VariableFeatures have not been set for any object in input."/> | |
| 38 | |
| 39 </inputs> | |
| 40 <outputs> | |
| 41 <data label="${tool.name} on ${on_string}: Rdata file with features" name="file_out" format='rdata' /> | |
| 42 </outputs> | |
| 43 <tests> | |
| 44 <!-- MANUAL TESTS --> | |
| 45 <test> | |
| 46 <param name="rds_seurat_file" ftype="rdata" value="ifnb_ctrl_norm_fvg.rds,ifnb_stim_norm_fvg.rds"/> | |
| 47 <output name="file_out" ftype="rdata" > | |
| 48 <assert_contents> | |
| 49 <has_size value="9506" delta="950"/> | |
| 50 </assert_contents> | |
| 51 </output> | |
| 52 </test> | |
| 53 <!-- END MANUAL TESTS --> | |
| 54 </tests> | |
| 55 <help> | |
| 56 <!-- MANUAL HELP --> | |
| 57 <![CDATA[ | |
| 58 .. class:: infomark | |
| 59 | |
| 60 **What it does** | |
| 61 | |
| 62 @SEURAT_INTRO@ | |
| 63 | |
| 64 This tool aims to facilitate the first steps of the Seurat 4.0.4 https://satijalab.org/seurat/articles/integration_introduction.html | |
| 65 tutorial, to select features for integration of different datasets. This features can be used then with the integration tool. | |
| 66 | |
| 67 All options are documented in-line. | |
| 68 | |
| 69 ----- | |
| 70 | |
| 71 **Inputs** | |
| 72 | |
| 73 * A set of Seurat objects (can be given in other formats as well) to integrate. These objects should be at least normalised and have the find variable genes/features method applied. | |
| 74 * All other inputs are optional (see above). | |
| 75 | |
| 76 ----- | |
| 77 | |
| 78 **Outputs** | |
| 79 | |
| 80 * A Seurat (or other format depending on selection) with the integrated object. | |
| 81 | |
| 82 @VERSION_HISTORY@ | |
| 83 ]]> | |
| 84 <!-- END MANUAL HELP --> | |
| 85 </help> | |
| 86 <expand macro="citations" /> | |
| 87 </tool> |
