Mercurial > repos > ecology > ecology_beta_diversity
view graph_link_var.r @ 0:fb7b2cbd80bb draft default tip
"planemo upload for repository https://github.com/Marie59/Data_explo_tools commit 60627aba07951226c8fd6bb3115be4bd118edd4e"
author | ecology |
---|---|
date | Fri, 13 Aug 2021 18:17:38 +0000 |
parents | |
children |
line wrap: on
line source
#Rscript ################################################ ## Link between variables and themselves ## ################################################ #####Packages : ggplot2 # Cowplot # Car # faraway # dplyr # GGally # FactoMiner # factoextra # ggcorrplot #####Load arguments args <- commandArgs(trailingOnly = TRUE) if (length(args) == 0) { stop("This tool needs at least one argument") }else{ table <- args[1] hr <- args[2] colli <- as.logical(args[3]) vif <- as.logical(args[4]) pca <- as.logical(args[5]) interr <- as.logical(args[6]) auto <- as.logical(args[7]) spe <- as.numeric(args[8]) col <- as.numeric(strsplit(args[9], ",")[[1]]) var <- as.numeric(args[10]) var2 <- as.numeric(args[11]) var4 <- as.numeric(args[12]) } if (hr == "false") { hr <- FALSE }else{ hr <- TRUE } if (length(col) == 1) { stop("Please select two or more numerical columns") } #####Import data data <- read.table(table, sep = "\t", dec = ".", header = hr, fill = TRUE, encoding = "UTF-8") if (vif | pca) { data_active <- data[col] #Define the active individuals and the active variables for the PCA } if (colli | interr) { colspe <- colnames(data)[spe] } if (colli) { data_num <- data[col] data_num$species <- data[, spe] data_num <- data_num[grep("^$", data_num$spe, invert = TRUE), ] } if (interr | auto) { colvar <- colnames(data)[var] } if (interr) { colvar2 <- colnames(data)[var2] colvar4 <- colnames(data)[var4] } #####Your analysis ####Independence of the observations#### acf_tb <- function(data, var) { obj <- acf(data[, var], na.action = na.pass) return(obj) } acf_df <- function(data, var) { tb <- data.frame(acf = acf_tb(data, var)$acf, lag = acf_tb(data, var)$lag) return(tb) # Lag: intervalle temporel entre mesures, fréquence à laquelle on mesure l'auto corrélation. # ACF: indépendance temporelle } autocorr <- function(var1, var2) { cat("\nACF\n", var2$acf, file = "acf.txt", fill = 1, append = TRUE) graph <- ggplot2::ggplot() + ggplot2::geom_bar(ggplot2::aes(x = var2$lag, y = var2$acf), stat = "identity", position = "identity", fill = "midnightblue") + ggplot2::geom_hline(mapping = ggplot2::aes(yintercept = qnorm((1 + 0.95) / 2) / sqrt(var1$n.used)), linetype = "dashed") + # calcul interval de confiance à 95% sans correction du bruit blanc. ggplot2::geom_hline(mapping = ggplot2::aes(yintercept = -qnorm((1 + 0.95) / 2) / sqrt(var1$n.used)), linetype = "dashed") + ggplot2::labs(title = "Autocorrelation") + ggplot2::xlab("lag") + ggplot2::ylab("acf") ggplot2::theme(plot.title = ggplot2::element_text(hjust = 0.5)) ggplot2::ggsave("autocorrelation.png", graph) } ####Interractions#### graph <- function(data, var1, var2, var3) { graph <- ggplot2::ggplot(data, ggplot2::aes_string(x = var1, y = var2, group = var3, color = var3)) + ggplot2::geom_point() + ggplot2::geom_smooth(method = lm, se = FALSE) + ggplot2::theme(plot.title = ggplot2::element_text(color = "black", size = 12, face = "bold")) return(graph) } # Put multiple panels interraction <- function(data, var1, var2, var3, var4) { cat("\nSpecies\n", spe, file = "Species.txt", fill = 1, append = TRUE) if (mult1) { for (spe in unique(data[, var3])) { data_cut <- data[data[, var3] == spe, ] mult_graph <- graph(data_cut, var1, var2, var3) + ggplot2::facet_grid(cols = ggplot2::vars(data_cut[, var4]), scales = "free") + cowplot::background_grid(major = "xy", minor = "none") + cowplot::panel_border() + ggplot2::ggtitle("Interactions") ggplot2::ggsave(paste("interaction_of_", spe, ".png"), mult_graph, width = 10, height = 7) } }else{ mult_graph <- graph(data, var1, var2, var3) + ggplot2::facet_grid(rows = ggplot2::vars(data[, var3]), cols = ggplot2::vars(data[, var4]), scales = "free") + cowplot::background_grid(major = "xy", minor = "none") + cowplot::panel_border() + ggplot2::ggtitle("Interactions") ggplot2::ggsave("interraction.png", mult_graph) } } ####Collinearity among covariates#### # Create the plots coli <- function(data, var) { if (mult2) { cat("\nThere is not enough data on these species they appear too few times in the tabular-file\n", file = "Data.txt", fill = 1, append = TRUE) for (spe in unique(data$species)) { nb_spe <- sum(data$species == spe) if (nb_spe <= 2) { cat(spe, file = "Data.txt", fill = 1, append = TRUE) }else{ data_cut <- data[data$species == spe, ] nb <- ncol(data_cut) data_num <- data_cut[, -nb] graph <- GGally::ggpairs(data_num, ggplot2::aes(color = data_cut$species), lower = list(continuous = "points"), axisLabels = "internal") ggplot2::ggsave(paste0("collinarity_of_", spe, ".png"), graph, width = 20, height = 15) } } }else{ nb <- ncol(data) data_cut <- data[, -nb] graph <- GGally::ggpairs(data_cut, ggplot2::aes(color = data[, var]), lower = list(continuous = "points"), axisLabels = "internal") + ggplot2::scale_colour_manual(values = c("#00AFBB", "#E7B800", "#FC4E07")) + ggplot2::scale_fill_manual(values = c("#00AFBB", "#E7B800", "#FC4E07")) ggplot2::ggsave("collinarity.png", graph) } } ####PCA method#### plot_pca <- function(data) { #Correlation circle graph_corr <- factoextra::fviz_pca_var(active_data(data), col.var = "cos2", gradient.cols = c("#00AFBB", "#E7B800", "#FC4E07"), repel = TRUE #Avoid text overlap ) ggplot2::ggsave("Pca_circle.png", graph_corr) } plot_qual <- function(data) { #PCA results for variables var <- factoextra::get_pca_var(active_data(data)) #representation quality graph_quality <- ggcorrplot::ggcorrplot(var$cos2[!apply(var$cos2, 1, anyNA), ], method = "circle", ggtheme = ggplot2::theme_gray, colors = c("#00AFBB", "#E7B800", "#FC4E07")) ggplot2::ggsave("Pca_quality.png", graph_quality) } #### Variance inflation factor #### myvif <- function(mod) { v <- vcov(mod) assign <- attributes(model.matrix(mod))$assign if (names(coefficients(mod)[1]) == "(Intercept)") { v <- v[-1, -1] assign <- assign[-1] } else warning("No intercept: vifs may not be sensible.") terms <- labels(terms(mod)) n_terms <- length(terms) if (n_terms < 2) stop("The model contains fewer than 2 terms") if (length(assign) > dim(v)[1]) { diag(tmp_cor) <- 0 if (any(tmp_cor == 1.0)) { return("Sample size is too small, 100% collinearity is present") } else { return("Sample size is too small") } } r <- cov2cor(v) detr <- det(r) result <- matrix(0, n_terms, 3) rownames(result) <- terms colnames(result) <- c("GVIF", "Df", "GVIF^(1/2Df)") for (term in 1:n_terms) { subs <- which(assign == term) result[term, 1] <- det(as.matrix(r[subs, subs])) * det(as.matrix(r[-subs, -subs])) / detr result[term, 2] <- length(subs) } if (all(result[, 2] == 1)) { result <- data.frame(GVIF = result[, 1]) } else { result[, 3] <- result[, 1] ^ (1 / (2 * result[, 2])) } invisible(result) } corvif1 <- function(dataz) { dataz <- as.data.frame(dataz) #correlation part tmp_cor <- cor(dataz, use = "complete.obs") return(tmp_cor) } corvif2 <- function(dataz) { dataz <- as.data.frame(dataz) #vif part form <- formula(paste("fooy ~ ", paste(strsplit(names(dataz), " "), collapse = " + "))) dataz <- data.frame(fooy = 1, dataz) lm_mod <- lm(form, dataz) return(myvif(lm_mod)) } #Autocorrelation if (auto) { obj1 <- acf_tb(data, var = colvar) obj2 <- acf_df(data, var = colvar) autocorr(var1 = obj1, var2 = obj2) } if (interr) { #Interractions mult1 <- ifelse(length(unique(data[, colspe])) <= 6, FALSE, TRUE) interraction(data, var1 = colvar, var2 = colvar2, var3 = colspe, var4 = colvar4) } #Collinearity if (colli) { mult2 <- ifelse(length(unique(data[, spe])) < 3, FALSE, TRUE) coli(data = data_num, var = spe) } #PCA if (pca) { active_data <- function(data) { #Calcul of PCA for the active data res_pca <- FactoMineR::PCA(data, graph = FALSE) return(res_pca) } #eigenvalue eig_val <- capture.output(factoextra::get_eigenvalue(active_data(data_active))) cat("\nwrite table with eigenvalue. \n--> \"", paste(eig_val, "\"\n", sep = ""), file = "valeurs.txt", sep = "", append = TRUE) plot_pca(data_active) plot_qual(data_active) } #VIF if (vif) { #Compute 2 tables# tb_corr <- as.data.frame(corvif1(dataz = data_active)) tb_corr <- cbind(x = rownames(tb_corr), tb_corr) tb_vif <- corvif2(dataz = data_active) write.table(tb_corr, "corr.tabular", row.names = FALSE, quote = FALSE, sep = "\t", dec = ".", fileEncoding = "UTF-8") if (all(is.na(tb_vif))) { tb_vif <- NULL cat("Vif couldn't be calculated, selected data isn't correlated") }else{ write.table(tb_vif, "vif.tabular", row.names = FALSE, quote = FALSE, sep = "\t", dec = ".", fileEncoding = "UTF-8") } }