# HG changeset patch # User edward-kirton # Date 1307482049 14400 # Node ID 38e2b656eb2808daa99aa5cc35a0b6327ff96cad Migrated tool version 1.0.1 from old tool shed archive to new tool shed repository diff -r 000000000000 -r 38e2b656eb28 lucy/lucy.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lucy/lucy.xml Tue Jun 07 17:27:29 2011 -0400 @@ -0,0 +1,62 @@ + +psub -l galaxy_normal.c --fasta $fasta_infile --cat $fasta_outfile --cat $qual_outfile +lucy -quiet -xtra 8 +-error $max_avg_error $max_error_at_ends +-bracket $bracket_window_size $bracket_max_avg_error +#if $vector.vector_select == '1': +-vector $vector.vector_fasta_infile $vector.splice_fasta_infile +#end if +-output $fasta_outfile $qual_outfile +$fasta_infile $qual_infile + + + + + + + + + + + + + + + + + + + + + + + + + + + + +Less Useful Chunks Yank (lucy) is a utility that prepares raw DNA sequence fragments for sequence assembly. +It performs quality end-trimming and optionally removal of primer/vector sequences or filtering contamination. + +--- + +Each sequence in the output sequence file begins with a header that includes its name, three pass along +clone length values to the fragment assembly program, and a left and right marker denoting the begin and +end of the good quality, vector free region. The following is an example of lucy output:: + + >GCCAA03TF 1500 3000 2000 43 490 + AGCCAAGTTTGCAGCCCTGCAGGTCGACTCTAGAGGATCCCCAGGATGATCAGCCACATT + GGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGG + GCGAAAGCCTGACGCAGCCATGCCGCGTGAATGATGAAGGTCTTAGGATTGTAAAATTCT + TTCACCGGGGACGATAATGACGGTACCCGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGC + ... + +--- + +**Manual** + +http://galaxy.jgi-psf.org/static/manuals/lucy.pdf + + + diff -r 000000000000 -r 38e2b656eb28 lucy/lucy_wrapper.sh --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lucy/lucy_wrapper.sh Tue Jun 07 17:27:29 2011 -0400 @@ -0,0 +1,2 @@ +#!/bin/bash +$* 2>&1