Mercurial > repos > eschen42 > w4mcorcov
annotate w4mcorcov.xml @ 8:342570ad880c draft
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
author | eschen42 |
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date | Sat, 04 Aug 2018 17:43:16 -0400 |
parents | 066b1f409e9f |
children | 06c51af11531 |
rev | line source |
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8
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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1 <tool id="w4mcorcov" name="OPLS-DA_Contrasts" version="0.98.12"> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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2 <description>OPLS-DA Contrasts of Univariate Results</description> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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3 <macros> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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4 <xml name="paramPairSigFeatOnly"> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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5 <param name="pairSigFeatOnly" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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6 label="Retain only pairwise-significant features" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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7 help="When this option is set to 'Yes', analysis will be performed including only features that differ significantly for the pair of levels being contrasted; when set to 'No', any feature that varies significantly across all levels will be included (i.e., exclude any feature that is not significantly different across all levels). See examples below." /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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8 </xml> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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9 <xml name="cplots"> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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10 <param name="cplot_y" label="C-plot Y-axis" type="select" help="Choose the Y-axis for C-plots."> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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11 <option value="correlation">Plot VIP versus correlation</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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12 <option value="covariance">Plot VIP versus covariance</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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13 </param> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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14 <param name="cplot_p" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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15 label="Produce predictor C-plot" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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16 help="When this option is set to 'Yes', correlation will be plotted against vip4 for predictor loadings." /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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17 <param name="cplot_o" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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18 label="Produce orthogonal C-plot" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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19 help="When this option is set to 'Yes', correlation will be plotted against vip4 for orthogonal loadings." /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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20 </xml> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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21 </macros> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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22 <requirements> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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23 <requirement type="package">r-batch</requirement> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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24 <requirement type="package">bioconductor-ropls</requirement> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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25 </requirements> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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26 <command detect_errors="aggressive"><![CDATA[ |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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27 Rscript '$__tool_directory__/w4mcorcov_wrapper.R' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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28 dataMatrix_in '$dataMatrix_in' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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29 sampleMetadata_in '$sampleMetadata_in' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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30 variableMetadata_in '$variableMetadata_in' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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31 facC '$facC' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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32 #if str( $signif_test.tesC ) == "none": |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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33 tesC "none" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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34 pairSigFeatOnly "FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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35 #else: |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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36 tesC '$signif_test.tesC' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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37 pairSigFeatOnly '$signif_test.pairSigFeatOnly' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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38 #end if |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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39 levCSV '$levCSV' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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40 matchingC '$matchingC' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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41 labelFeatures '$labelFeatures' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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42 #if str( $xplots.expPlot ) == "none": |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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43 cplot_p "FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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44 cplot_o "FALSE" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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45 cplot_y "correlation" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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46 #else if str( $xplots.expPlot ) == "cplot": |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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47 cplot_p '$xplots.cplot_p' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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48 cplot_o '$xplots.cplot_o' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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49 cplot_y '$xplots.cplot_y' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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50 #end if |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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51 contrast_detail '$contrast_detail' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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52 contrast_corcov '$contrast_corcov' |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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53 contrast_salience '$contrast_salience' |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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54 ]]></command> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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55 |
8
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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56 <inputs> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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57 <param name="dataMatrix_in" type="data" format="tabular" label="Data matrix file" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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58 help="Features x samples (tabular data - decimal: '.'; missing: NA; mode: numerical; separator: tab character)" /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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59 <param name="sampleMetadata_in" type="data" format="tabular" label="Sample metadata file" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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60 help="Samples x metadata (tabular data - decimal: '.'; missing: NA; mode: character or numerical; separator: tab character)" /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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61 <param name="variableMetadata_in" type="data" format="tabular" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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62 label="Variable metadata file (ideally from Univariate)" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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63 help="Features x metadata (tabular data - decimal: '.'; missing: NA; mode: character or numerical; separator: tab character)" /> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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64 <param name="facC" type="text" |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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65 label="Factor of interest" |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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66 help="REQUIRED - The name of the column of sampleMetadata corresponding to the qualitative variable used to define the contrasts. Except when the 'Univariate Significance-test' is set to 'none', this also must be a portion of the column names in the variableMetadata file."/> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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67 <conditional name="signif_test"> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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68 <param name="tesC" label="Univariate significance-test" type="select" help="Either 'none' or the name of the statistical test that was run by the 'Univariate' tool to produce the variableMetadata file; that name must also be a portion of the column names in that file."> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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69 <option value="none">none - Display all features from variableMetadata (rather than choosing a subset based on significance in univariate testing)</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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70 <option value="ttest">ttest - Student's t-test (parametric test, qualitative factor with exactly 2 levels)</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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71 <option value="anova">anova - Analysis of variance (parametric test, qualitative factor with more than 2 levels)</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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72 <option value="wilcoxon">wilcoxon - Wilcoxon rank test (nonparametric test, qualitative factor with exactly 2 levels)</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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73 <option value="kruskal">kruskal - Kruskal-Wallis rank test (nonparametric test, qualitative factor with more than 2 levels)</option> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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74 </param> |
342570ad880c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 0c81f4f1416129dd7061042b12cd28c0b8927aab
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75 <when value="none" /> |
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76 <when value="ttest"> |
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77 <expand macro="paramPairSigFeatOnly" /> |
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78 </when> |
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79 <when value="anova"> |
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80 <expand macro="paramPairSigFeatOnly" /> |
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81 </when> |
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82 <when value="wilcoxon"> |
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83 <expand macro="paramPairSigFeatOnly" /> |
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84 </when> |
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85 <when value="kruskal"> |
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86 <expand macro="paramPairSigFeatOnly" /> |
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87 </when> |
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88 </conditional> |
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89 <param name="levCSV" type="text" value="*" label="Levels of interest" |
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90 help="Comma-separated level-names (or comma-less regular expressions to match level-names) to consider in analysis; must match at least two levels; levels must be non-numeric; may include wild cards or regular expressions. Note that extra space characters will affect results - 'a,b' is correct, but 'a , b' is not and may fail or give different results."> |
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91 <sanitizer> |
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92 <valid initial="string.letters"> |
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93 <add preset="string.digits"/> |
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94 <add value="$" /> <!-- $ dollar, dollar-sign --> |
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95 <add value="(" /> <!-- ( left-paren --> |
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96 <add value=")" /> <!-- ) right-paren --> |
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97 <add value="*" /> <!-- * splat, asterisk --> |
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98 <add value="+" /> <!-- + plus --> |
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99 <add value="," /> <!-- , comma --> |
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100 <add value="-" /> <!-- - dash, minus-sign --> |
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101 <add value="." /> <!-- . dot, period --> |
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102 <add value=":" /> <!-- : colon --> |
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103 <add value=";" /> <!-- ; semi, semicolon --> |
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104 <add value="?" /> <!-- ? what, question mark --> |
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105 <add value="[" /> <!-- [ l-squib, left-squre-bracket --> |
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106 <add value="\" /> <!-- \ whack, backslash --> |
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107 <add value="]" /> <!-- ] r-squib, right-squre-bracket --> |
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108 <add value="^" /> <!-- ^ hat, caret --> |
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109 <add value="{" /> <!-- { l-cube, left-curly-bracket --> |
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110 <add value="|" /> <!-- | pipe --> |
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111 <add value="}" /> <!-- } r-cube, right-curly-bracket --> |
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112 <!-- IMPORTANT - Note that single and double quotes are not part of this list; they have the potential to make the 'command' section insecure or broken. --> |
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113 </valid> |
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114 </sanitizer> |
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115 </param> |
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116 <param name="matchingC" label="Level-name matching" type="select" help="How to specify level-names generically. (See help below for details on using wild cards or regular expressions.)"> |
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117 <option value="none">do no generic matching (default)</option> |
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118 <option value="wildcard" selected="true">use wild-cards for matching level-names</option> |
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119 <option value="regex">use regular expressions for matching level-names</option> |
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120 </param> |
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121 <param name="labelFeatures" type="text" value="3" |
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122 label="How many features having extreme loadings should be labelled on cov-vs.-cor plot" |
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123 help="Specify the number of features at each of the loading-extremes that should be labelled (with the name of the feature) on the covariance-vs.-correlation plot; specify 'ALL' to label all features or '0' to label no features; this choice has no effect on the OPLS-DA loadings plot."/> |
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124 <conditional name="xplots"> |
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125 <param name="expPlot" label="Extra plots to include" type="select" help="Choosing 'none' hides further choices."> |
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126 <option value="none">Do not include additonal extra plots.</option> |
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127 <option value="cplot">Include C-plots (predictor-loading vs. 'vip4p' and orthogonal-loading versus 'vip4o')</option> |
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128 </param> |
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129 <when value="none" /> |
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130 <when value="cplot"> |
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131 <expand macro="cplots" /> |
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132 </when> |
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133 </conditional> |
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134 </inputs> |
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135 <outputs> |
0
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136 <!-- |
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137 pdf1: summaries of each contrasts, clearly labelled by level=pair name |
0
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138 * first PCA score-plot |
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139 * then PLS score-plot |
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140 * then PLS S-PLOT; color in red features with VIP > 1; color in grey any non-pairwise-significant features, if these are included |
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141 --> |
8
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142 <data name="contrast_detail" format="pdf" label="${tool.name}_${variableMetadata_in.name}_detail" /> |
0
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143 <!-- |
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144 tsv1: cor and cov table with columns: |
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145 * feature-ID |
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146 * factor-level 1 |
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147 * factor-level 2, lexically greater than level 1 |
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148 * Wiklund_2008 correlation |
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149 * Wiklund_2008 covariance |
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150 * Galindo_Prieto_2014 VIP for predictive components, VIP[4,p] |
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151 * Galindo_Prieto_2014 VIP for orthogonal components, VIP[4,o] |
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152 * (When filtering on significance of univariate tests) Significance of test of null hypothesis that there is no difference between the two classes, i.e, the pair-wise test. |
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153 --> |
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154 <data name="contrast_corcov" format="tabular" label="${tool.name}_${variableMetadata_in.name}_corcov" /> |
0
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155 <!-- |
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156 tsv2: salience table with columns (experimental feature): |
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157 * feature-ID |
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158 * Salient level, i.e., for the feature, the class-level having the greatest median intensity |
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159 * Salient robust coefficient of variation, i.e., for the feature, the mean absolute deviation of the intensity for the salient level divided by the median intensity for the salient level |
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160 * Salience, i.e., for the feature, the median of the class-level having the greatest intensity divided by the mean of the medians for all class-levels. |
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161 --> |
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162 <data name="contrast_salience" format="tabular" label="${tool.name}_${variableMetadata_in.name}_salience" /> |
0
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163 </outputs> |
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164 <tests> |
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165 <!-- test #1 --> |
0
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166 <test> |
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167 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/> |
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168 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/> |
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169 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/> |
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170 <param name="tesC" value="kruskal"/> |
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171 <param name="facC" value="k10"/> |
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172 <param name="pairSigFeatOnly" value="FALSE"/> |
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173 <param name="labelFeatures" value="3"/> |
0
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174 <param name="levCSV" value="k[12],k[3-4]"/> |
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175 <param name="matchingC" value="regex"/> |
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176 <output name="contrast_corcov"> |
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177 <assert_contents> |
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178 <!-- column-labels line --> |
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179 <has_text text="featureID" /> |
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180 <has_text text="factorLevel1" /> |
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181 <has_text text="factorLevel2" /> |
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182 <has_text text="correlation" /> |
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183 <has_text text="covariance" /> |
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184 <has_text text="vip4p" /> |
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185 <has_text text="vip4o" /> |
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186 <has_text text="level1Level2Sig" /> |
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187 <!-- first matched line --> |
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188 <has_text text="M349.2383T700" /> |
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189 <has_text text="-0.3704185" /> |
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190 <has_text text="-36.6668927" /> |
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191 <has_text text="0.4914638" /> |
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192 <has_text text="0.01302117" /> |
0
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193 <!-- second matched line --> |
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194 <has_text text="M207.9308T206" /> |
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195 <has_text text="0.3235022" /> |
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196 <has_text text="5.97529097" /> |
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197 <has_text text="0.207196379" /> |
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198 <has_text text="0.04438632" /> |
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199 </assert_contents> |
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200 </output> |
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201 <output name="contrast_salience"> |
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202 <assert_contents> |
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203 <!-- column-labels line --> |
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204 <has_text text="featureID" /> |
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205 <has_text text="salientLevel" /> |
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206 <has_text text="salientRCV" /> |
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207 <has_text text="salience" /> |
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208 <!-- first matched line --> |
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209 <has_text text="M349.2383T700" /> |
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210 <has_text text="0.659554" /> |
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211 <has_text text="8.81866595" /> |
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212 <!-- second matched line --> |
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213 <has_text text="M207.9308T206" /> |
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214 <has_text text="0.0578578" /> |
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215 <has_text text="2.27527985" /> |
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216 <!-- third matched line --> |
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217 <has_text text="M211.0607T263" /> |
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218 <has_text text="9999" /> |
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219 <has_text text="12.87766096" /> |
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220 </assert_contents> |
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221 </output> |
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222 </test> |
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223 <!-- test #2 --> |
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224 <test> |
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225 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/> |
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226 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/> |
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227 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/> |
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228 <param name="tesC" value="kruskal"/> |
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229 <param name="facC" value="k10"/> |
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230 <param name="pairSigFeatOnly" value="TRUE"/> |
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231 <param name="labelFeatures" value="3"/> |
0
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232 <param name="levCSV" value="k[12],k[3-4]"/> |
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233 <param name="matchingC" value="regex"/> |
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234 <output name="contrast_corcov"> |
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235 <assert_contents> |
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236 <!-- column-labels line --> |
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237 <has_text text="featureID" /> |
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238 <has_text text="factorLevel1" /> |
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239 <has_text text="factorLevel2" /> |
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240 <has_text text="correlation" /> |
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241 <has_text text="covariance" /> |
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242 <has_text text="vip4p" /> |
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243 <has_text text="vip4o" /> |
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244 <has_text text="level1Level2Sig" /> |
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245 <!-- first matched line --> |
6
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246 <has_text text="M200.005T296" /> |
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247 <has_text text="-0.24533821" /> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit e73fabe1b3c871abbcb2e89914c181149c8e2066
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248 <has_text text="-3.3573953" /> |
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249 <has_text text="0.1157346" /> |
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250 <has_text text="0.0647860" /> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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251 </assert_contents> |
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252 </output> |
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253 <output name="contrast_salience"> |
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254 <assert_contents> |
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255 <!-- column-labels line --> |
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256 <has_text text="featureID" /> |
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257 <has_text text="salientLevel" /> |
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258 <has_text text="salientRCV" /> |
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259 <has_text text="salience" /> |
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260 <!-- first matched line --> |
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261 <has_text text="M349.2383T700" /> |
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262 <has_text text="0.659554" /> |
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263 <has_text text="8.81866595" /> |
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264 <!-- second matched line --> |
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265 <has_text text="M207.9308T206" /> |
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266 <has_text text="0.0578578" /> |
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267 <has_text text="2.27527985" /> |
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268 <!-- third matched line --> |
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269 <has_text text="M211.0607T263" /> |
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270 <has_text text="9999" /> |
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271 <has_text text="12.87766096" /> |
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272 </assert_contents> |
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273 </output> |
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274 </test> |
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275 <!-- test #3 --> |
0
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276 <test> |
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277 <param name="dataMatrix_in" value="input_dataMatrix.tsv"/> |
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278 <param name="sampleMetadata_in" value="input_sampleMetadata.tsv"/> |
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279 <param name="variableMetadata_in" value="input_variableMetadata.tsv"/> |
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280 <param name="tesC" value="none"/> |
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281 <param name="facC" value="k10"/> |
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282 <param name="labelFeatures" value="3"/> |
0
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283 <param name="levCSV" value="k[12],k[3-4]"/> |
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284 <param name="matchingC" value="regex"/> |
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285 <output name="contrast_corcov"> |
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286 <assert_contents> |
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287 <!-- column-labels line --> |
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288 <has_text text="featureID" /> |
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289 <has_text text="factorLevel1" /> |
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290 <has_text text="factorLevel2" /> |
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291 <has_text text="correlation" /> |
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292 <has_text text="covariance" /> |
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293 <has_text text="vip4p" /> |
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294 <has_text text="vip4o" /> |
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295 <!-- first matched line --> |
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296 <has_text text="M349.2383T700" /> |
7
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297 <has_text text="-0.37867079" /> |
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298 <has_text text="-37.71066" /> |
6
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299 <has_text text="0.5246766" /> |
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300 <has_text text="0.0103341" /> |
0
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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301 <!-- second matched line --> |
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302 <has_text text="M207.9308T206" /> |
7
066b1f409e9f
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit e73fabe1b3c871abbcb2e89914c181149c8e2066
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303 <has_text text="0.31570433" /> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit e73fabe1b3c871abbcb2e89914c181149c8e2066
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304 <has_text text="5.86655640" /> |
6
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305 <has_text text="0.2111623" /> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit cafda5095a79ce2376325b57337302f95137195d
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306 <has_text text="0.0488654" /> |
0
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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307 </assert_contents> |
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308 </output> |
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309 <output name="contrast_salience"> |
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310 <assert_contents> |
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311 <!-- column-labels line --> |
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312 <has_text text="featureID" /> |
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313 <has_text text="salientLevel" /> |
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314 <has_text text="salientRCV" /> |
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315 <has_text text="salience" /> |
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316 <!-- first matched line --> |
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317 <has_text text="M349.2383T700" /> |
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318 <has_text text="0.659554" /> |
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319 <has_text text="8.81866595" /> |
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320 <!-- second matched line --> |
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321 <has_text text="M207.9308T206" /> |
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322 <has_text text="0.0578578" /> |
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323 <has_text text="2.27527985" /> |
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324 <!-- third matched line --> |
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325 <has_text text="M211.0607T263" /> |
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326 <has_text text="9999" /> |
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327 <has_text text="12.87766096" /> |
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328 </assert_contents> |
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329 </output> |
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330 </test> |
6
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331 <!-- test #4 --> |
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332 <test> |
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333 <param name="dataMatrix_in" value="issue1_input_dataMatrix.tsv"/> |
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334 <param name="sampleMetadata_in" value="issue1_input_sampleMetadata.tsv"/> |
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335 <param name="variableMetadata_in" value="issue1_input_variableMetadata.tsv"/> |
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336 <param name="tesC" value="none"/> |
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337 <param name="facC" value="tissue_flowering"/> |
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338 <param name="labelFeatures" value="3"/> |
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339 <param name="levCSV" value="*"/> |
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340 <param name="matchingC" value="wildcard"/> |
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341 <output name="contrast_corcov"> |
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342 <assert_contents> |
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343 <!-- column-labels line --> |
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344 <has_text text="featureID" /> |
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345 <has_text text="factorLevel1" /> |
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346 <has_text text="factorLevel2" /> |
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347 <has_text text="correlation" /> |
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348 <has_text text="covariance" /> |
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349 <has_text text="vip4p" /> |
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350 <has_text text="vip4o" /> |
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351 <!-- first matched line --> |
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352 <has_text text="NM516T251" /> |
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353 <has_text text="flower_yes" /> |
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354 <has_text text="other" /> |
7
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355 <has_text text="0.03402807" /> |
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356 <has_text text="0.03526926" /> |
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357 <has_text text="0.43664386" /> |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit e73fabe1b3c871abbcb2e89914c181149c8e2066
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358 <has_text text="0.587701897" /> |
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359 <has_text text="0.026082688" /> |
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360 <has_text text="0.0437742145" /> |
6
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361 <has_text text="516.0845" /> |
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362 <has_text text="250.8762" /> |
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363 </assert_contents> |
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364 </output> |
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365 <output name="contrast_salience"> |
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366 <assert_contents> |
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367 <!-- column-labels line --> |
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368 <has_text text="featureID" /> |
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369 <has_text text="salientLevel" /> |
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370 <has_text text="salientRCV" /> |
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371 <has_text text="salience" /> |
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372 <has_text text="mz" /> |
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373 <has_text text="rt" /> |
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374 <!-- first matched line --> |
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375 <has_text text="PM518T369" /> |
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376 <has_text text="flower_yes" /> |
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377 <has_text text="0.58655260" /> |
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378 <has_text text="4.414469" /> |
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379 <has_text text="518.1656" /> |
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380 <has_text text="368.59817" /> |
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381 </assert_contents> |
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382 </output> |
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383 </test> |
0
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384 </tests> |
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385 <help><![CDATA[ |
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386 |
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387 **Run PLS-DA Contrasts of Univariate Results** |
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388 ---------------------------------------------- |
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389 |
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390 **Author** - Arthur Eschenlauer (University of Minnesota, esch0041@umn.edu) |
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391 |
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392 |
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393 Motivation |
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394 ---------- |
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395 |
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396 OPLS-DA and the SIMCA S-PLOT (Wiklund *et al.*, 2008) may be employed to draw attention to metabolomic features that are potential biomarkers, i.e. features that are potentially useful to discriminate to which class a sample should be assigned (e.g. Sun *et al.*, 2016). Workflow4Metabolomics (W4M, Giacomoni *et al.*, 2014, Guitton *et al.*, 2017) provides a suite of tools for preprocessing and statistical analysis of LC-MS, GC-MS, and NMR metabolomics data; however, it does not (as of release 3.0) include a tool for making the equivalent of an S-PLOT. |
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397 |
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398 The S-PLOT is computed from mean-centered, pareto-scaled data. This plot presents the correlation of the first score vector from an OPLS-DA model with the sample-variables used to produce that model versus the covariance of the scores with the sample-variables. For OPLS-DA, the first score vector represents the variation among the sample-variables that is related to the predictor (i.e., the contrasting factor). |
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399 |
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400 The primary aims of this tool are: |
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401 |
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402 - To compute and visualize multiple contrasts with OPLS-DA and the covariance vs. correlation plot. |
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403 - To write the results to data files for use in further multivariate analysis or visualization. |
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404 |
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405 Note: This tool only supports categorical factors with non-numeric level-names. |
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406 |
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407 Description |
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408 ----------- |
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409 |
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410 The purpose of the 'PLS-DA Contrasts' tool is to visualize GC-MS or LC-MS features that are possible biomarkers. |
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411 |
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412 The W4M 'Univariate' tool (Th]]>é<![CDATA[venot *et al.*, 2015) adds the results of family-wise corrected pairwise significance-tests as columns of the **variableMetadata** dataset. |
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413 For instance, suppose that you ran Kruskal-Wallis testing for a column named 'cluster' in sampleMetadata that has values 'k1' and 'k2' and at least one other value. |
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414 |
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415 - A column of variableMetadata would be labelled 'cluster_kruskal_sig' and would have values '1' and '0'; when the samples are grouped by 'cluster', '1' means that there is strong evidence against the hypothesis that there is no difference among the intensities for the feature across all sample-groups. |
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416 - A column of variableMetadata would be labelled 'cluster_kruskal_k1.k2_sig' and would have values '1' and '0', where '1' means that there is significant evidence against the hypothesis that samples from sampleMetadata whose 'cluster' column contains 'k1' or 'k2' have the same intensity for that feature. |
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417 |
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418 The 'PLS-DA Contrasts' tool produces graphics and data for OPLS-DA contrasts of feature-intensities between significantly different pairs of factor-levels. For each factor-level, the tool performs a contrast with all other factor-levels combined and then separately with each other factor-level. |
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419 |
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420 **Along the left-to-right axis, the plots show the supervised projection of the variation explained by the predictor** (i.e., the factor specified when invoking the tool); **the top-to-bottom axis displays the variation that is orthogonal to the predictor level** (i.e., independent of it). |
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421 |
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422 Although this tool can be used in a purely exploratory manner by supplying the variableMetadata file without the columns added by the W4M 'Univariate' tool, **the preferred workflow may be to use univariate testing to exclude features that are not significantly different and then to use OPLS-DA to visualize the differences identified in univariate testing** (Th]]>é<![CDATA[venot *et al.*, 2015); an appropriate exception would be to visualize contrasts of a specific list of metabolites. |
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423 |
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424 It must be stressed that there may be no *single* definitive computational approach to select features that are reliable biomarkers, especially from a small number of samples or experiments. A few possible choices are: |
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425 |
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426 - picking features with maximum loadings along the projection parallel to the predictor (loadp), |
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427 - examining extreme values on S-PLOTs (for which covariance is linearly related to loadp), |
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428 - examining "variable importance in projection VIP for OPLS-DA" (Galindo-Prieto *et al.* 2014), and |
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429 - examining a feature's "selectivity ratio" (Rajalahti *et al.*, 2009). |
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430 |
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431 In this spirit, this tool reports the S-PLOT covariance and correlation (Wiklund *op. cit.*) and VIP metrics, and it introduces an informal "salience" metric to flag features that may merit attention without dimensional reduction; future versions may add selectivity ratio. |
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432 |
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433 For a more systematic approach to biomarker identification, please consider the W4M 'biosigner' tool (Rinuardo *et al.* 2016), which applies three different identification metrics to the selection process. |
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434 |
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435 Regardless of how any potential biomarker is identified, further validation analysis (e.g., independent confirmatory experiments) is needed before it is recommended for general application. |
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436 |
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437 |
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438 W4M Workflow Position |
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439 --------------------- |
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440 |
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441 - Upstream tool: **Univariate** (category: Statistical Analysis) or (not generally recommended) any **Preprocessing** tool that produces or updates a 'variableMetadata' file. |
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442 - Downstream tool categories: **Statistical Analysis** |
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443 |
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444 Input files |
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445 ----------- |
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446 |
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447 +----------------------+-----------+ |
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448 | File | Format | |
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449 +======================+===========+ |
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450 | Data matrix | tabular | |
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451 +----------------------+-----------+ |
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452 | Sample metadata | tabular | |
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453 +----------------------+-----------+ |
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454 | Variable metadata | tabular | |
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455 +----------------------+-----------+ |
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456 |
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457 Output files |
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458 ------------ |
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459 |
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460 +-------------------------------------------+-----------+ |
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461 | File | Format | |
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462 +===========================================+===========+ |
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463 | Contrast detail | pdf | |
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464 +-------------------------------------------+-----------+ |
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465 | Contrast "corrlation and covariance" data | tabular | |
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466 +-------------------------------------------+-----------+ |
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467 | Feature "salience" data | tabular | |
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468 +-------------------------------------------+-----------+ |
0
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469 |
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470 Parameters |
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471 ---------- |
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472 |
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473 [IN] Data matrix file |
1
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474 | variable x sample **dataMatrix** (tabular separated values) file of the numeric data matrix, with '.' as decimal, and 'NA' for missing values; the table must not contain metadata apart from row and column names; the row and column names must be identical to the rownames of the sample and variable metadata, respectively (see below) |
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475 | |
0
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476 |
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477 [IN] Sample metadata file |
1
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478 | sample x metadata **sampleMetadata** (tabular separated values) file of the numeric and/or character sample metadata, with '.' as decimal and 'NA' for missing values |
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479 | |
0
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480 |
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481 [IN] Variable metadata file |
1
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482 | variable x metadata **variableMetadata** (tabular separated values) file of the numeric and/or character variable metadata, with '.' as decimal and 'NA' for missing values |
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483 | |
0
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484 |
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485 [IN] Test |
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486 | Name of the **statistical test** - a component of column names in variable metadata table |
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487 | May be one of 'none', 'ttest', 'gwilcoxon', 'anova', 'kruskal', 'pearson', 'spearman' |
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488 | |
0
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489 |
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490 [IN] Factor of interest |
1
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491 | Name of the **column of sampleMetadata** corresponding to the qualitative or quantitative variable |
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492 | |
0
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493 |
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494 [IN] Retain only pairwise-significant features |
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495 | *Note that when 'Test' is 'none', all features are included in the analysis and this parameter is not settable.* |
1
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496 | When **true**, for each contrast of two levels, include only those features which pass the significance threshold for that contrast. Choosing true results in an OPLS-DA model that better reflects and visualizes the difference detected by univariate analysis, with somewhat increased reliability of prediction (as assessed by cross-validation). |
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497 | When **false**, include all features that pass the significance threshold when testing for difference across all factor-levels. This choice produces a plot that displays more features but is not necessarily more informative. |
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498 | |
0
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499 |
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500 [IN] Levels of interest |
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501 | Comma-separated **level-names** (or comma-less regular expressions to match level-names) to consider in analysis; must match at least two levels; may include wild cards or regular expressions. |
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502 | |
0
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503 |
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504 [IN] Level-name matching |
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505 | Indicator of **how levels are to be specified generically** (if at all) - wild cards, regular expressions, or none (no generic matching). |
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506 | |
0
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507 |
2
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508 [IN] Label how many extreme features |
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509 | Specify the number of features at each of the loading-extremes that should be labelled (with the name of the feature) on the covariance-vs.-correlation plot; specify 'ALL' to label all features; this choice has no effect on the OPLS-DA loadings plot. |
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510 | |
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511 |
0
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512 [OUT] Contrast-detail output PDF |
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513 | Several plots for each two-projection OPLS-DA analysis: |
0
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514 |
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515 - (first row, left) **correlation-versus-covariance plot** of OPLS-DA results (a work-alike for the S-PLOT, computed using formula in Supplement to Wiklund, *op. cit.*); point-color becomes saturated as the "variable importance in projection to the predictive components" (VIP\ :subscript:`4,p` from Galindo-Prieto *et al.* 2014) ranges from 0.83 and 1.21 (Mehmood *et al.* 2012), for use to identify features for consideration as biomarkers. |
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516 - (second row, left) **model-overview plot** for the two projections; grey bars are the correlation coefficient for the fitted data; black bars indicate performance in cross-validation tests (Th]]>é<![CDATA[venot, 2017) |
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517 - (first row, right) OPLS-DA **scores-plot** for the two projections (Th]]>é<![CDATA[venot *et al.*, 2015) |
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518 - (second row, right) **correlation-versus-covariance plot** of OPLS-DA results **orthogonal to the predictor** (see section "S-Plot of Orthogonal Component" in Wiklund, *op. cit.*, pp. 120-121; this characterizes features with the greatest variation independent of the predictor). |
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519 - (third row, left, when "**predictor C-plot**" is chosen under "Extra plots to include") plot of the covariance or correlation vs. the VIP for the projection *parallel to the predictor*, for use to identify features for consideration as biomarkers. |
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520 - (third row, right, when "**orthogonal C-plot**" is chosen under "Extra plots to include") plotof the covariance or correlation vs. the VIP for the projection *orthogonal to the predictor*, for use to identify features varying considerably without regard to the predictor. |
0
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521 |
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522 [OUT] Contrast Correlation-Covarinace data TABULAR |
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523 | A tab-separated values file of metadata for each feature for each contrast in which it was included. |
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524 | Thus, a given feature may appear many times, but *the combination of featureID, factorLevel1, and factorLevel2 will be unique.* |
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525 | This file has the following columns: |
0
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526 |
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527 - **featureID** - feature-identifier |
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528 - **factorLevel1** - factor-level 1 |
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529 - **factorLevel2** - factor-level 2 (or "other" when contrasting factor-level 1 with all other levels) |
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530 - **correlation** - correlation of the features projection explaining the difference between the features, < 0 when intensity for level 1 is greater (from formula in Supplement to Wiklund, *op. cit.*). Note that, for a given contrast, there is a linear relationship between 'loadp' and 'correlation'. |
0
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531 - **covariance** - covariance of the features projection explaining the difference between the features, < 0 when intensity for level 1 is greater (from formula in *ibid.*) |
1
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532 - **vip4p** - "variable importance in projection" to the predictive projection, VIP\ :subscript:`4,p` (Galindo-Prieto *op. cit.*) |
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533 - **vip4o** - "variable importance in projection" to the orthogonal projection, VIP\ :subscript:`4,o` (*ibid.*) |
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534 - **loadp** - variable loading for the predictive projection (Wiklund *op. cit.*) |
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535 - **loado** - variable loading for the orthogonal projection (*ibid.*) |
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536 - **level1Level2Sig** - (Only present when a test other than "none" is chosen) '1' when feature varies significantly across all classes (i.e., not pair-wise); '0' otherwise |
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537 |
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538 [OUT] Feature "Salience" data TABULAR |
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539 | Metrics for the "salient level" for each feature, i.e., the level at which the feature is more prominent than any other level. This is *not* at all related to the SIMCA OPLS-DA S-PLOT; rather, it is intended as a potential way to discover features for consideration as potential biomarkers without dimensionally reducting the data. This is a tab-separated values file having the following columns: |
0
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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540 |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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541 - **featureID** - feature identifier |
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542 - **salientLevel** - salient level, i.e., for the feature, the class-level having the greatest median intensity |
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543 - **salientRCV** - salient robust coefficient of variation, i.e., for the feature, the mean absolute deviation of the intensity for the salient level divided by the median intensity for the salient level |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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544 - **salience** - salience, i.e., for the feature, the median of the class-level having the greatest intensity divided by the mean of the medians for all class-levels |
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545 |
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546 Wild card patterns to match level-names |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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547 --------------------------------------- |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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548 |
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549 "wild card" patterns may be used to select level-names. |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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550 |
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551 - use '``?``' to match a single character |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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552 - use '``*``' to match zero or more characters |
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553 - the entire pattern must match the level name |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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554 |
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555 For example |
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556 |
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557 - '``??.le*``' matches '``my.level``' but not '``my.own.level``' |
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558 - '``*.level``' matches '``my.level``' and '``my.own.level``' |
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559 - '``*.level``' matches neither '``my.level``' nor '``my.own.level``' |
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560 |
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561 Regular expression patterns to match level-names |
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562 ------------------------------------------------ |
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563 |
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564 "regular expression" patterns may be used to select level-names. |
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565 |
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566 POSIX 1003.2 standard regular expressions allow precise pattern-matching and are exhaustively defined at: |
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567 http://pubs.opengroup.org/onlinepubs/9699919799/basedefs/V1_chap09.html |
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568 |
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569 However, only a few basic building blocks of regular expressions need to be mastered for most cases: |
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570 |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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571 - '``^``' matches the beginning of a level-name |
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572 - '``$``' matches the end of a level-name |
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573 - '``.``' outside of square brackets matches a single character |
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574 - '``*``' matches character specified immediately before zero or more times |
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575 - Square brackets specify a set of characters to be matched. Within square brackets: |
0
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576 |
1
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577 - '``^``' as the first character specifies that the list of characters are those that should **not** be matched. |
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578 - '``-``' is used to specify ranges of characters |
0
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579 |
1
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580 Caveat: The tool wrapper uses the comma ('``,``') to split a list of sample-level names, so **commas may not be used within regular expressions for this tool.** |
0
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581 |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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582 First Example: Consider a field of level-names consisting of '``marq3,marq6,marq9,marq12,front3,front6,front9,front12``' |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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583 |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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584 - The regular expression '``^front[0-9][0-9]*$``' will match the same sample-levels as '``front3,front6,front9,front12``' |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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585 - The regular expression '``^[a-z][a-z]3$``' will match the same sample-levels as '``front3,marq3``' |
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586 - The regular expression '``^[a-z][a-z]12$``' will match the same sample-levels as '``front12,marq12``' |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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587 - The regular expression '``^[a-z][a-z][0-9]$``' will match the same sample-levels as '``front3,front6,front9,marq3,marq6,marq9``' |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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588 |
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589 Second Example: Consider these regular expression patterns as possible matches to a sample-level name '``AB0123``': |
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590 |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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591 - '``^[A-Z][A-Z][0-9][0-9]*$``' - MATCHES '``**^AB0123$**``' |
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592 - '``^[A-Z][A-Z]*[0-9][0-9]*$``' - MATCHES '``**^AB0123$**``' |
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593 - '``^[A-Z][0-9]*``' - MATCHES '``**^A** B0123$``' - first character is a letter, '``*``' can specify zero characters, and end of line did not need to be matched. |
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594 - '``^[A-Z][A-Z][0-9]``' - MATCHES '``**^AB0** 123$``' - first two characters are letters aind the third is a digit. |
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595 - '``^[A-Z][A-Z]*[0-9][0-9]$``' - NO MATCH - the name does not end with the pattern '``[A-Z][0-9][0-9]$``', i.e., it ends with four digits, not two. |
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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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596 - '``^[A-Z][0-9]*$``' - NO MATCH - the pattern specifies that second character and all those that follow, if present, must be digits. |
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597 |
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598 Working examples |
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599 ---------------- |
0
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600 |
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601 **Input files** |
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602 |
4
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603 +-------------------------------------------------------------------------------------------------------------------+ |
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604 | Download from URL | |
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605 +===================================================================================================================+ |
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606 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_dataMatrix.tsv | |
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607 +-------------------------------------------------------------------------------------------------------------------+ |
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608 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_sampleMetadata.tsv | |
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609 +-------------------------------------------------------------------------------------------------------------------+ |
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610 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_variableMetadata.tsv | |
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diff
changeset
|
611 +-------------------------------------------------------------------------------------------------------------------+ |
1
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
612 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
613 **Example 1:** Include in the analysis only features identified as pair-wise significant in the Univariate test. |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
614 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
615 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
616 | Input Parameter or Result | Value | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
617 +============================================+========================================================================================================================================+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
618 | Factor of interest | k10 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
619 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
620 | Univariate Significance-Test | kruskal | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
621 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
622 | Retain only pairwise-significant features | Yes | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
623 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
624 | Levels of interest | k[12],k[3-4] | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
625 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
626 | Level-name matching | use regular expressions for matching level-names | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
627 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
628 | Number of features having extreme loadings | ALL | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
629 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
630 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
631 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
632 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
633 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
634 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail.pdf | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
635 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
636 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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changeset
|
637 **Example 2:** Include in the analysis only features identified as overall-significant in the Univariate test. Note that this even includes these features in contrasts where they were not determined to be pair-wise significant in the Univariate test. Thus, more features are included than in Example 1. |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
638 |
1
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
639 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
640 | Input Parameter or Result | Value | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
641 +============================================+========================================================================================================================================+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
642 | Factor of interest | k10 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
643 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
644 | Univariate Significance-Test | kruskal | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
645 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
646 | Retain only pairwise-significant features | No | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
647 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
648 | Levels of interest | ``*`` | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
649 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
650 | Level-name matching | use wild cards for matching level-names | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
651 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
652 | Number of features having extreme loadings | 5 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
653 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
654 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_all.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
655 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
656 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_all.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
657 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
658 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_all.pdf | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
659 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
660 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
661 **Example 3:** Include all features in the analysis without regard to Univariate testing. Univariate testing is not even a pre-requisite to using the tool when 'none' is selected for the test. Thus, more features are included than in Example 2. |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
662 |
1
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
663 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
664 | Input Parameter or Result | Value | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
665 +============================================+========================================================================================================================================+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
666 | Factor of interest | k10 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
667 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
668 | Univariate Significance-Test | none | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
669 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
670 | Levels of interest | k[12],k[3-4] | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
671 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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diff
changeset
|
672 | Level-name matching | use regular expressions for matching level-names | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
673 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
674 | Number of features having extreme loadings | 0 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
675 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
676 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_global.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
677 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
678 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_global.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
679 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
680 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_global.pdf | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
681 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
682 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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diff
changeset
|
683 **Example 4:** Analysis of a two-level factor (including all features). This suppresses the contrasts of "each factor vs. the aggregate of all the others". |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
684 |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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|
685 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
686 | Input Parameter or Result | Value | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
687 +============================================+========================================================================================================================================+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
688 | Factor of interest | lohi | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
689 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
690 | Univariate Significance-Test | none | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
691 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
692 | Levels of interest | low,high | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
693 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
694 | Level-name matching | use regular expressions for matching level-names | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
695 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
696 | Number of features having extreme loadings | 3 | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
697 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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|
698 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_lohi.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
699 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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|
700 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_lohi.tsv | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
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changeset
|
701 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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|
702 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_lohi.pdf | |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
0
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|
703 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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changeset
|
704 |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
705 |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
706 Trademarks |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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|
707 ---------- |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
708 |
4
8bba31f628da
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 8f2dc8b66666340275cd8967e09c504720528462
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diff
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|
709 OPLS-DA, SIMCA, and S-PLOT are registered trademarks of the Umetrics company. http://umetrics.com/about-us/trademarks |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
710 |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
711 |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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changeset
|
712 ]]></help> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
713 <citations> |
5
50f60f94c034
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit aff1790e25523d038a1e9528de748191c096132f
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parents:
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diff
changeset
|
714 <!-- this tool --> |
2
e03582f26617
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 7682e8e7ae2bfb926d94b414b9a1649389f33582
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parents:
1
diff
changeset
|
715 <citation type="doi">10.5281/zenodo.1034784</citation> |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
716 <!-- Galindo_Prieto_2014 Variable influence on projection (VIP) for OPLS --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
717 <citation type="doi">10.1002/cem.2627</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
718 <!-- Giacomoni_2014 W4M 2.5 --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
719 <citation type="doi">10.1093/bioinformatics/btu813</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
720 <!-- Guitton_2017 W4M 3.0 --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
721 <citation type="doi">10.1016/j.biocel.2017.07.002</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
diff
changeset
|
722 <!-- Mehmood_2012 PLS-based variable-selection --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
723 <citation type="doi">10.1186/1748-7188-6-27</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
724 <!-- Rajalahti_2009 Biomarker discovery using selectivity ratio --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
725 <citation type="doi">10.1016/j.chemolab.2008.08.004</citation> |
1
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
726 <!-- Rinuardo 2016 --> |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
0
diff
changeset
|
727 <citation type="doi">10.3389/fmolb.2016.00026</citation> |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
728 <!-- Sun_2016 Urinary Biomarkers for adolescent idiopathic scoliosis --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
729 <citation type="doi">10.1038/srep22274</citation> |
4
8bba31f628da
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 8f2dc8b66666340275cd8967e09c504720528462
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parents:
3
diff
changeset
|
730 <!-- Thevenot_2015 Urinary metabolome statistics --> |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
731 <citation type="doi">10.1021/acs.jproteome.5b00354</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
diff
changeset
|
732 <!-- ropls package --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
733 <citation type="bibtex"><![CDATA[ |
1
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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|
734 @incollection{Thevenot_ropls_2017, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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diff
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|
735 author = {Th{\'{e}}venot, Etienne A.}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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diff
changeset
|
736 title = {ropls: PCA, PLS(-DA) and OPLS(-DA) for multivariate analysis and feature selection of omics data}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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diff
changeset
|
737 publisher = {bioconductor.org}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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diff
changeset
|
738 year = {2017}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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|
739 doi = {10.18129/B9.bioc.ropls}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
eschen42
parents:
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changeset
|
740 booktitle = {Bioconductor: Open source software for bioinformatics}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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|
741 address = {Roswell Park Cancer Institute}, |
0c2ad44b6c9c
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit 01d4a951cf09e7b88fcec96b8043bc7568cc5c92
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parents:
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changeset
|
742 } |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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|
743 ]]></citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
eschen42
parents:
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changeset
|
744 <!-- Wiklund_2008 OPLS PLS-DA and S-PLOT --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
745 <citation type="doi">10.1021/ac0713510</citation> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
746 </citations> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
747 <!-- |
5
50f60f94c034
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit aff1790e25523d038a1e9528de748191c096132f
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parents:
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diff
changeset
|
748 vim:noet:sw=2:ts=2 |
0
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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parents:
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changeset
|
749 --> |
23f9fad4edfc
planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit bd26542b811de06c1a877337a2840a9f899c2b94
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|
750 </tool> |