Mercurial > repos > fabio > iwtomics
diff plotwithscale.xml @ 18:565118df598a draft
Uploaded 20170531
author | fabio |
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date | Wed, 31 May 2017 11:07:22 -0400 |
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children | d49031ef33d5 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plotwithscale.xml Wed May 31 11:07:22 2017 -0400 @@ -0,0 +1,201 @@ +<tool id="plotwithscale" name="IWTomics Plot with Threshold" version="@VERSION@.0"> + <description>on Test Scale</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <stdio> + <exit_code range="-1" source="both" level="fatal" description="Missing IWTomics package. Please be sure to have it installed before using this tool." /> + <exit_code range="10" source="both" level="fatal" description="Wrong test ids." /> + <exit_code range="20" source="both" level="fatal" description="Wrong feature ids." /> + <exit_code range="30" source="both" level="fatal" description="Two scale thresholds selected for the same test and feature." /> + <exit_code range="40" source="both" level="fatal" description="Scale threshold too high." /> + <exit_code range="50" source="both" level="fatal" description="Group by 'test' but selected features with different resolution." /> + <exit_code range="60" source="both" level="fatal" description="Summary plot error. Please try again." /> + <regex match="Error" source="both" level="fatal" description="An error has occurred. Please try again." /> + </stdio> + + <command> +<![CDATA[ + Rscript $__tool_directory__/plotwithscale.R adjustedpvalue="${adjustedpvalue}" iwtomicsrespdf="${iwtomicsrespdf}" iwtomicssumpdf="${iwtomicssumpdf}" + iwtomicsrdata="${rdata}" + iwtomicstests="${testids}" + iwtomicsselectedfeatures="${featureids}" + + #set test_subset = '"\\|"'.join( [ str( $e.test ) for $e in $scalesection.thresholdontestscale ] ) + test_subset="${test_subset}" + #set feature_subset = '"\\|"'.join( [ str( $e.feature ) for $e in $scalesection.thresholdontestscale ] ) + feature_subset="${feature_subset}" + #set scale_subset = '","'.join( [ str( $e.scale ) for $e in $scalesection.thresholdontestscale ] ) + scale_subset="c(${scale_subset})" + + testalpha="${plotres.alpha}" + average="${plotres.average}" + size="${plotres.size}" + plottype="'${plotres.conditionalplottype.plottype}'" + #if $plotres.conditionalplottype.plottype == 'boxplot': + #set probs = '","'.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] ) + #if $probs != "": + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})" + #else: + probs="c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})" + #end if + #end if + + groupby="'${plotsum.conditionalgroupby.groupby}'" + #if $plotsum.conditionalgroupby.groupby == "test": + summaryalpha="${plotsum.conditionalgroupby.testalphaplot}" + only_significant="${plotsum.conditionalgroupby.testonlysig}" + #elif $plotsum.conditionalgroupby.groupby == "feature": + summaryalpha="${plotsum.conditionalgroupby.featurealphaplot}" + only_significant="${plotsum.conditionalgroupby.featureonlysig}" + #end if + + >& /dev/null +]]> + <!-- + to print the stack add the following line at the end of the command and enable the corresponding entry in output + 2> "${stackerr}" + --> + </command> + + <inputs> + <help> + ... + </help> + + <!-- RData --> + <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object with test results" help="File created by 'IWTomics Test and Plot'." /> + <!-- test IDs --> + <param format="tabular" name="testids" type="data" label="Select test IDs" help="File created by 'IWTomics Test and Plot'." /> + <!-- feature IDs --> + <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Test and Plot'." /> + + <!-- repeat threshold on test scale --> + <section name="scalesection" title="Select scale for Interval-Wise Testing and plot" expanded="True" help="Maximum interval length for the p-value adjustment. If 0 the maximum possible scale is used."> + <repeat name="thresholdontestscale" title="Threshold on test scale" min="1"> + <param name="test" type="data_column" data_ref="testids" numerical="False" label="Test ID" multiple="True" use_header_names="True" /> + <param name="feature" type="data_column" data_ref="featureids" numerical="False" label="Feature ID" multiple="True" use_header_names="True" /> + <param name="scale" type="integer" value="0" min="0" label="Scale" /> + </repeat> + </section> + + <!-- plot IWTomics results --> + <section name="plotres" title="Plot IWTomics test results" expanded="True"> + <!-- alpha --> + <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <!-- average --> + <param name="average" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot the mean curves" /> + <!-- average --> + <param name="size" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="True" label="Plot sample size in each position" /> + <!-- conditional plot type --> + <conditional name="conditionalplottype"> + <!-- plot type --> + <param name="plottype" type="select" label="Plot type"> + <option value="boxplot">Pointwise quantile curves (boxplot)</option> + <option value="curves">Curves (aligned)</option> + </param> + <!-- conditional choice: plottype=boxplot --> + <when value="boxplot"> + <section name="probabilitiessection" title="Probabilities" expanded="True" help="Probabilities corresponding to the quantile curves to be drawn."> + <param name="prob0" size="3" type="float" value="0.25" min="0.0" max="1.0" label="Probability" /> + <param name="prob1" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + <param name="prob2" size="3" type="float" value="0.75" min="0.0" max="1.0" label="Probability" /> + <repeat name="probabilities" title="Probabilities"> + <param name="prob" size="3" type="float" value="0.5" min="0.0" max="1.0" label="Probability" /> + </repeat> + </section> + </when> + </conditional> + </section> + + <!-- summary plot --> + <section name="plotsum" title="Summary plot" expanded="True"> + <!-- conditional group by --> + <conditional name="conditionalgroupby"> + <!-- group by --> + <param name="groupby" type="select" label="Group by" help="How tests should be grouped."> + <option value="none">No plot</option> + <option value="test">Group by test</option> + <option value="feature">Group by feature</option> + </param> + <when value="test"> + <param name="testalphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="testonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + <when value="feature"> + <param name="featurealphaplot" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" /> + <param name="featureonlysig" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Plot only significant tests" /> + </when> + </conditional> + </section> + </inputs> + + <outputs> + <!--<data format="txt" name="stackerr" label="iwtomics.testandplot.stackerr.txt" from_work_dir="iwtomics.testandplot.stackerr.txt" />--> + <data format="txt" name="adjustedpvalue" label="${tool.name} on ${on_string}: Adjusted p-value" from_work_dir="iwtomics.testandplot.adjustedpvalue.txt" /> + <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.testandplot.iwtomicstestresults.pdf" /> + <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.testandplot.summaryplot.pdf" /> + </outputs> + + <help> +This tool allows to select the scale for the Interval-Wise Testing results. In particular, it returns the p-value curves +for the different tests performed at the selected scale, and it creates a graphical representation of the +Interval-Wise Testing results and a summary plot (optional) at the selected scale. + +----- + +**Input files** + +RData file with the IWTomicsData object with test results, tabular files with test IDs and feature IDs. +These files are created by the tool *IWTomics Test and Plot*. + +----- + +**Output** + +The tool returns: + +1. TXT file with an adjusted p-value curve for every test performed at the selected scale; +2. PDF file with the plotted test results; +3. PDF file with the summary plot. + +----- + +.. class:: infomark + +**Notes** + +This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA). + +It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects. +The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_). + +.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf + </help> + + <citations> + <citation type="bibtex"> + @ARTICLE{ + iwt-functional-data, + author = {A Pini and S. Vantini}, + title = {Interval-Wise Testing for functional data}, + journal = {Journal of Nonparametric Statistics}, + year = {2017}, + volume = {29}, + number = {2}, + pages = {407-424} + } + </citation> + <citation type="bibtex"> + @MANUAL{ + iwtomics, + author = {Cremona, Marzia A and Pini, Alessia and Chiaromonte, Francesca and Vantini, Simone}, + title = {IWTomics: Interval-Wise Testing for Omics Data}, + note = {R package version 0.99.12}, + year = {2017} + } + </citation> + </citations> + +</tool>